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The Effect of Metabolic State on Perilipin 5/5b Expression
No full textThe drastic increase in lipid-associated metabolic disorders has led to an increasing necessity to study the PAT family of proteins. These proteins, which coat the surface of fat droplets inside of the cell, lead to the binding of lipids to various organs which can cause health problems. Previous research has found that one of these proteins, Perilipin 5 (PLIN5), plays a role in the hepatic fat accumulation that can lead to Non-Alcoholic Fatty Liver Disease (NAFLD). The increasing prevalence of NAFLD is concerning due to its ability to progress into hepatocellular carcinoma which is one of the most fatal types of cancer. A splice variant of PLIN5 has been detected and termed Perilipin 5b (PLIN5b). It is currently unknown if one’s metabolic condition can affect the presence or absence of the splice variant. Using mice that have been fed and fasted, the difference in the concentration or the presence of the splice variant was examined under varying metabolic states. The difference in concentrations between PLIN5 and its splice variant could correlate to the abundance of lipids that have not been broken down in the liver and the development of NAFLD. In this project, western blots were ran using the tissues from the mice that had been varied metabolically. Both PLIN5 and PLIN5b were found in the tissues; however, differences were found in the size of the splice variant which gives evidence for different post-translational modifications between different metabolic states
Development of Evidence-Based Clinical Practice Guidelines for Pediatric Patients at Risk of Emergence Delirium
Get PDFPostoperative emergence agitation or emergence delirium (ED) is a phenomenon that can occur in patients who have undergone surgery requiring the administration of inhaled anesthetics. ED is a postoperative side effect of general anesthesia commonly experienced by pediatric patients that negatively impacts a patient’s overall postoperative experience. There is a lack of consistent standardized clinical practice guidelines for general anesthesia in pediatric or adult populations. The primary goal of this scholarly project is to develop and implement clinical practice guidelines focusing on the occurrence of postoperative ED and select adverse clinical outcomes in pediatric patients undergoing general anesthesia. The project utilizes the Plan, Do, Study, Act (PDSA) framework to evaluate the outcomes of ED. The guidelines include pharmacological and non-pharmacological interventions addressing treatment options encompassing the preoperative, intraoperative, and postoperative care areas. The projects’ objective is to provide EBP guidelines of pharmacological and non-pharmacological perioperative treatment options for pediatric ED to reduce the overall incidence and select adverse clinical outcomes. The outcomes from this scholarly project can be fundamental to recommending EBP guidelines to other pediatric facilities that are struggling with the negative consequences associated with ED
Molecular Sex Determination in a Segregating Population of Ginkgo biloba Seedlings
No full textGinkgo biloba L. (GINKGOACEAE) is one of the oldest known tree species and is the sole representative of the monotypic genus Ginkgo. Ginkgo trees are dioecious (i.e., separate, individual male and female trees) and take \u3e20 years to reach sexual maturity. Female trees produce a seed enclosed in the fleshy and fruit like sarcotesta that emits a putrid smell when mature. Because of the smell, male trees are preferred in urban settings. The ginkgo industry propagates trees clonally via grafting or stem cuttings to insure male plants at the expense of genetic diversity. In contrast, female ginkgo trees have been reported to produce higher levels of pharmaceutically relevant flavonoids; hence, genetically diverse female populations of ginkgo are an interest to the pharmaceutical industry. This study aimed to develop a method to accurately determine the sexual identity of ginkgo seedlings with a PCR-based approach. The methods are based on the amplification of a 1,030-bp sex determining region of GbMADS18 located on chromosome 2. The reliability of the PCR-based sex marker was confirmed with eight mature female and four mature male individuals whose sexual identity was determined phenotypically. The PCR-based approach was then used to screen a population of 30 seedlings for sexual identity. Based on the presence/absence of the 1,030 bp band, the population was comprised of 12 males and 18 females and did not differ from an expected 1:1 ratio of males to females (X2 = 1.2, df=1). These results indicate that a molecular determination of sexual identity based on the amplification of GbMADS18 gene correlates with phenotypic sexing of mature ginkgo trees and can predict the sexual identity of immature seedlings
Otterbein Aegis Spring 2024
Get PDFContents: Editors’ Introduction , Editorial Board Members Essays; “Dress as a Weapon”,“ Kubrick’s Survivors: Breaking the Cycle of Violence in Stanley Kubrick’s Killer’s Kiss and The Shining”, “The French Muslim Debate”, “The Hole and The New Minimalist Object”, “The Loneliness of Womanhood”, “ The Road Towards Sustainable Resource Management in the Brazilian Amazon and Beyond”, “Understanding Desire in Stanley Kubrick’s Eyes Wide Shut” Book Reviews; Cultish: The Language of Fanaticism, Esmond and Ilia: An Unreliable Memoir, Fetch the Bolt Cutters, I’m Glad My Mom Died, My Body, North Woods, Only the Clothes on Her Back, The Art of Darkness: A Treasury of the Morbid, Melancholic, and Macabre, The Case for Rage, The Daughter of Doctor Moreau, The Dead Wrestler Elegies, The Power of the Brush, The Witch’s Heart, To Shake the Sleeping Selfhttps://digitalcommons.otterbein.edu/aegis_humanity/1020/thumbnail.jp
The Injury Risks of Sport Specialization on Division III College Athletes
No full textA single-sport athlete or early sport specialization (ESS) are defined as those who commit themselves to one sport for more than 8 months out of the year. The hope is the athlete will develop better sport specific skills faster than their counterparts. However, ESS has shown to have an association with increased mental health concerns, burn out rates, and injury. The goal of this research study was to determine if there is a correlation between early sport specialization and the total number of injuries experienced by a Division III athlete. In a descriptive research design, student-athletes at Otterbein University participated in a subjective survey about their experiences with sport specialization in both high school and college. Along with basic demographic information, athletes were asked to provide information on the type and frequency of any injuries sustained. Descriptive statistics were used to determine statistical significance of the survey data. There was no statistical significance found between the number of sports played or participants\u27 gender and the total number of injuries that an athlete experienced in high school t(44) = 0.08, p =.94. or in college t(44) = 1.26, p=0.21 . There were, however, differences seen in the frequency of different types of injuries experienced in high school t(44) = -1.22, p = 0.23 when compared to college t(44) = 0.90, p = 0.93. . While prior literature has found connections between early sport specialization and an increased injury risk, this research study did not come to the same conclusion. Based off the data found in this study, ESS does not increase the risk of injury for Division III athletes. However, more research needs to be done in this population
Guidelines for Non-Anesthesia Providers Performing Point of Care Ultrasound (POCUS) for Airway Assessment in the Emergency Room
Get PDFEffective airway management is critical for nurse anesthetists, encompassing tasks such as anesthesia administration, airway establishment, and patient safety, particularly in challenging cases where airway difficulties may arise. The subjectivity in airway assessments poses challenges for clinicians when accurately predicting difficult cases. Point-of-care ultrasound (POCUS) has emerged as a valuable tool for quantifying airway parameters, providing objective data to guide decision-making. This project aims to establish evidence-based guidelines for managing the airway in unconscious trauma patients requiring intubation using POCUS. By quantifying parameters such as PEP/E-VC ratios, hyomental distance, thyrohyoid membrane thickness, and anterior neck thickness, healthcare providers can make more informed decisions and potentially reduce malpractice settlements associated with anesthesia-related complications. The project follows the John Hopkins Evidence-Based Practice (JHEBP) Model, a systematic approach for incorporating research findings into patient care. Data collection will involve retrospective chart analysis to assess compliance in documenting airway parameters and using POCUS in difficult cases. The project will monitor CL grade III or IV classifications in patient records to identify trends and evaluate the application of POCUS in managing difficult airways. The timeline spans seven months, including planning, education, training, and data analysis. The ER staff will use a portable handheld ultrasound device, Vscan Air, to efficiently perform scans on all ER patients. This project seeks to enhance airway management in unconscious trauma patients, improve clinical outcomes, and reduce the risk of adverse events, ultimately benefiting anesthesia and emergency room providers and patients
A Midsummer Night\u27s Dream (2024)
No full texthttps://digitalcommons.otterbein.edu/theatre_dance_gallery/5755/thumbnail.jp
A Midsummer Night\u27s Dream (2024)
No full texthttps://digitalcommons.otterbein.edu/theatre_dance_gallery/5757/thumbnail.jp
Beehive: The \u2760s Musical (2024)
No full texthttps://digitalcommons.otterbein.edu/theatre_dance_gallery/5795/thumbnail.jp
It\u27s a Wonderful Life: A Live Radio Play
Get PDFhttps://digitalcommons.otterbein.edu/summer_production_2024/1002/thumbnail.jp