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Transportation Mechanism of Perilipin 5 into the Nucleus
No full textDiseases caused by excess lipid content in the body continue to increase in prevalence in recent years. Type II diabetes, heart disease, obesity, and non-alcoholic fatty liver disease (NAFLD) are particularly harmful to the body. Lipids are stored in structures called lipid storage droplets that consist of a monolayer of phospholipids that surround pools of triglyceride molecules. Perilipin proteins are associated with the metabolism of lipid molecules and tend to localize on the outside of these lipid droplets. Perilipin 5, plays a large role in lipolysis of liver, heart, and skeletal muscle tissues. To do this, perilipin 5 migrates to the nucleus of the cell after being activated by protein kinase A (PKA). The mechanism of which perilipin 5 uses to enter the nucleus is largely unknown. Understanding this nuclear transportation mechanism could potentially lead to lipolysis regulation treatments for individuals with excess lipid content and minimize the likelihood of lipid related disease. Drugs such as mifepristone and ivermectin have been shown to inhibit importin, an important molecule in cellular protein migration (Lundberg et al., 2013). To probe the mechanism utilized by perilipin 5, Chinese hamster ovary (CHO) cells were treated with differing combinations of nuclear transportation inhibitors mifepristone and ivermectin, and activation compounds Forskolin and 3-Isobutyl-1-methylxanthine (IBMX). Immunofluorescence was then conducted to monitor the presence of perilipin 5 around the nucleus. Results of these treatments were found to be inconclusive. It is currently unknown whether mifepristone and ivermectin successfully inhibit nuclear transportation of perilipin 5
A Novel Role for a Two-Component Regulatory System in Bacterial Cell Division
No full textStreptomyces coelicolor is a Gram-positive bacterial species from the genus Streptomyces, part of the largest bacterial phylum called Actinobacteria. Streptomyces species are renowned for their ability to produce antibiotics, including vancomycin, streptomycin, and chloramphenicol, making them highly valued in microbiology research. Uniquely, Streptomyces can continue to grow even after the deletion of critical cell division genes, such as ftsZ and ftsQ, which would typically halt septum formation in other bacterial species, resulting in death. These unusual characteristics highlight Streptomyces\u27 versatility and resilience in surviving without cell division, presenting an intriguing subject for further study.
We have previously isolated several mutants that contain second-site suppressor mutations of an ftsQ-null S. coelicolor strain. The mutations can suppress the defects caused by the deletion of ftsQ, thereby restoring cell division and altering the bacterial colony color from blue to red. The primary objective of this research is to identify and characterize the ftsQ-null suppressor mutations and the corresponding genes along with their potential roles in cell division.
Previously our lab used whole genome sequencing to reveal that three of the different second-site suppressor mutations of the S. coelicolor ftsQ-null mutation were most likely located in the same gene. This gene is part of a two-component regulatory system that has not been associated with cell division previously. To aid in this field\u27s advancement, we have extended experiments to showcase the potential for finding additional suppressors that have almost equal capabilities or have more efficiency than the original suppressor. These new findings can help us contribute to understanding bacterial cell division mechanisms and examine a novel role for the two-component system in cell division
An Inquiry-Based Interdisciplinary Social Studies Unit on Libraries Around the World in a Second-Grade Classroom: A Curriculum Development
Full text linkThis project explores inquiry-based learning and project-based learning. My specific interest in interdisciplinary, inquiry-based instruction stemmed from professional development received, and from an overarching initiative to move towards this type of instruction within the school building that I work. The curriculum developed provides a two-week lesson sequence that can be implemented within a second-grade classroom. The lesson sequence is inquiry-based, and provides opportunities for students to communicate, collaborate, create, and think critically throughout the learning process. This curriculum is interdisciplinary in nature and is designed to meet second-grade Ohio social studies standards, as well as standards in other areas. The curriculum created allows students to broaden their understanding of “libraries around the world” and gives them a chance to create a library to serve a specific community
The Multilingual Book Club Model: Engaging Multilingual Learners in Bilingual (Spanish-English) Graphic Novel Book Clubs to Acquire Language and Vocabulary
No full textThis project explores the importance of using translanguaging to support multilingual learners in predominantly English speaking classrooms. The main intent is to provide an engaging curriculum that provides culturally responsive material for multilingual students whose home language is Spanish. The project explores seminal research in second language acquisition and the importance of vocabulary instruction. It also acknowledges the importance of the Book Club Mode
Footloose: The Musical
No full texthttps://digitalcommons.otterbein.edu/theatre_dance_gallery/5852/thumbnail.jp
The Glass Menagerie
No full texthttps://digitalcommons.otterbein.edu/theatre_dance_gallery/5874/thumbnail.jp
Otterbein Towers Spring 2025
No full textAround the \u27Bein; Brandon Koons \u2794, MAT\u2798, P\u2723: Coach, Role Model, Author; The Otterbein Advantage; Become Here: Graduating Seniors are READY; Forever Friends: The Cardinal Legacy; Coalition for the Good of Otterbein; Philanthropy; Alumni Matters; Class Notes.https://digitalcommons.otterbein.edu/towers/1283/thumbnail.jp
Final Scholarly Project: The Administration of Transversus Abdominis Plane Blocks in Patients Undergoing Anesthesia for Open Ventral Hernia Repairs
Full text linkVentral hernia repairs are the only definitive treatment for umbilical or abdominal hernias. These procedures are prevalent and often lifesaving but also carry high risks of adverse effects. Some of the problems that follow include postoperative ileus, increased opioid use, and prolonged length of stay. Due to the rate of complications, there was a need to improve these outcomes. One way to improve outcomes is to implement superior anesthetic techniques. This Final Scholarly Project (FSP) followed the Plan-Do-Study-Act (PDSA) model to conduct evidence-based practice (EBP) and quality improvement (QI) sections. The plan phase included a thorough literature search and analysis and the design of recommendations. Current literature shows that the administration of transversus abdominis plane (TAP) blocks in patients undergoing open ventral hernia repairs decreases total opioid use and reduces length of hospital stay. The recommendation includes an ultrasound-guided technique to administer a TAP block in the operating room before incision. The TAP block will consist of liposomal bupivacaine and normal saline. There will be 60 milliliters of the anesthetic administered bilaterally. The Do phase includes the proposed implementation of the recommendations, which include training and communication with stakeholders. The Study phase will include collection of data and analysis of outcomes. The measured outcomes will be total opioid use and length of hospital stay. The Act phase will include adjusting the recommendation as necessary to achieve the best possible patient outcomes. The goal of the project is to improve outcomes for patients undergoing ventral hernia surgery by implementing EBP strategies
Evidence-Based Practice Guidelines for Perioperative Use of Dexmedetomidine in Pediatric Congenital Cardiac Surgery
Full text linkTachyarrhythmias are common postoperatively in the congenital cardiac surgery population. About 1% of the population is born with congenital heart disease (CHD), with 50% of patients undergoing surgery for CHD experiencing arrhythmias. Complications can arise from postoperative arrhythmias, including hemodynamic instability and myocardial depression, having catastrophic health repercussions. Despite the high prevalence and complications, there is no standard practice for postoperative tachyarrhythmia prevention in the chosen population, and prevention is at the provider\u27s discretion. However, literature exploring the effects of dexmedetomidine notes promising results for using the drug for postoperative tachyarrhythmia prevention. The project aims to address the lack of evidence-based guidelines for tachyarrhythmia prevention in CHD cardiac surgery patients by implementing evidence-based practice guidelines for the intraoperative use of dexmedetomidine. The project utilizes quantitative data to evaluate and assess the effectiveness of the guideline in a level one pediatric trauma center. Following a review of literature, data collection, and data evaluation, the evidence-based guidelines for intraoperative use of dexmedetomidine are effective in reducing the incidence of postoperative tachyarrhythmias in congenital cardiac surgery patients, as well as a reduction in heart rate and invasive ventilation time. Utilizing the explored guidelines can lead to better patient outcomes, fewer complications, and a universal prevention protocol for anesthesia providers, providing consistently better results
Urinetown: The Musical
No full texthttps://digitalcommons.otterbein.edu/theatre_dance_gallery/5925/thumbnail.jp