13610 research outputs found
Sort by
Developing a Protocol for Bacteriophage Guided Evolution
No full textBacteriophage therapy is being explored as an alternative to antibiotic use due to an increase in antibiotic resistance. Expansion of phage host ranges is important for future phage therapy. A protocol was developed based upon the Appelmans Protocol to expand the host range of selected Gordonia bacteriophage. A spectrophotometer was used to determine lysis in a 96-well plate and confirm the success of the methods. A 100 phage to 1 bacteria concentration ratio was found to be effective. Two methods of bacteria concentration calculations were compared, one using a countable plate and the other using a spectrophotometer. The findings of the experiments were applied to a final protocol, which is followed by instructions on publishing research
Quantification of Gordonia terrae Bacteriophages using Lysate qPCR
No full textBacteriophages are viruses that infect and replicate only in bacterial cells. Quantitative PCR was used in this experiment to count the number of DNA copies in the phage lysate sample. In this project, the more rapid lysate qPCR method was compared to standard plate count titer assays using the double layer agar method, to match the number of genomic copies with the plaque forming units in the lysate. Initially, multiple primer sets were designed to amplify the best primers and reaction conditions to use for phage detection. Because qPCR is sensitive to contamination, there were multiple controls to ensure no contamination. Subsequently, a standard curve was generated by comparing the phage concentration determined from titer assays with that measured using SYBR Green qPCR. The optimized lysate qPCR method will be a faster method to determine the number of bacteriophage DNA copies in phage lysate, allowing the rapid determination of phage concentration for optical density-based infection growth curve experiments
Using OncoDB\u27s Oncovirus Analysis for HBV-related HCC
Full text linkSince one of the known causes of cancer is virus infection causing changes in gene regulation, being able to compare the expression and methylation of regular tumors and virus-related tumors could provide much needed information into virus induced cancer such as HBV-related HCC. OncoDB\u27s oncovirus analysis capabilities can provide information and research opportunities that past research has not had
Synthesis of ZnTPP-IL as a Potential Photodynamic Therapy Agent
Full text linkPhotodynamic Therapy is a new technique used in cancer treatment involving the use of a photosensitizer—a porphyrin—and light exposure to kill malignant cells. In this research, ZnTPP-IL was used as a photosensitizer with white light as a possible source of treatment of NIH 3T3 fibroblast and A549 lung cancer cell lines, with preliminary DNA testing on MYC-22 and G-quadruplex samples. ZnTPP-IL was synthesized by the reaction of the porphyrin ZnTPPC with isoleucinol. The new porphyrin was then purified by column chromatography using Sephadex LH-20 and G-50. ZnTPP-IL was characterized through NMR, IR, UV-vis, and fluorescence spectroscopies. The purity of the compound was then determined using HPLC. Additionally, ROS production using ZnTPP-IL was determined. The cytotoxicity of ZnTPP-IL was then examined by analyzing MTT assay with A549 lung cancer cell lines when paired with white and red light
The History of Dog Training and Its Importance to Modern Day Training Techniques
Full text linkThe world of dog training is a vast community that consists of many different methodologies, trainers, and philosophies, each with its own strengths and weaknesses. It is vital for any dog trainer, whether simply training their pet dog to come and sit or training a high-performance military working dog, to have a thorough understanding of today’s training philosophies and the philosophies that came before. Dog training is a living, breathing science that must be viewed as the sum of experiences and movements
Julia Smith Taylor papers
Full text linkThese papers are a collection of material pertaining to the life, career, hobbies, volunteer work, and family history of Julia Smith Taylor, who is a native of Arkadelphia, Arkansas and was voice teacher at Arkadelphia High School. The papers contain correspondence, photographs, newspaper clippings, audiovisual material, church records, and physical objects that are related to her and her family’s involvement with local clubs, churches, schools, and organizations
Ouachita announces 2024 Stepping Up honorees
Full text linkOuachita Baptist University will host its annual Stepping Up for Ouachita luncheon Friday, Nov. 8, at Chenal Country Club in Little Rock. The event will honor Susan Doshier of Frisco, Texas; Shari Edwards of North Little Rock, Ark.; Carol Miller Gresham of Springdale, Ark.; and Kathy Rainwater of Little Rock, Ark. Ticket and sponsorship information is available at obu.edu/steppingup.
Each year during Stepping Up for Ouachita, the university honors women alumni or friends of Ouachita who have demonstrated grace, leadership, integrity and faith
Kluck takes on his latest leadership challenge
Full text linkDr. Wesley Kluck did not have a restful 2020. As university physician and vice president for student development at Ouachita, he led the university’s health efforts in response to COVID-19, chairing Ouachita’s Health Monitoring and Action Team (HMAT).
“The worst thing was waking up in the middle of the night, thinking of things,” he said. “There was a lot of that. Sometimes I would get up and make a PowerPoint real quick, just type it all down and go back to sleep.”
HMAT, a group of 13 faculty and staff members as well as the Student Senate president, worked through a planning guide of more than 625 items to prepare Ouachita for on-campus learning in the fall, collaborating with colleagues across campus to check off each one. It’s no wonder he couldn’t sleep
