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Knowledge, awareness, and molecular epidemiology of fasciolosis in dairy cattle slaughtered in three commercial abattoirs in Eastern Cape Province, South Africa
Flukes and snail-borne diseases present significant challenges to livestock production, particularly impacting the dairy industry in the Eastern Cape Province, South Africa. Despite the challenges faced by the dairy sector, the level of knowledge and awareness among dairy farm personnel regarding the prevalence of bovine fasciolosis is unknown in Eastern Cape Province. Additionally, the genomic sequence of Fasciola spp. in dairy cattle slaughtered under abattoir conditions remains undisclosed. The primary objective of the titled study is to evaluate the knowledge, awareness, and molecular epidemiology of fasciolosis in dairy cattle slaughtered in three commercial abattoirs in the Eastern Cape province of South Africa. A structured questionnaire was prepared and randomly administered to 152 dairy farm personnel to investigate the knowledge and awareness of the aetiology, risk factors, and clinical signs of bovine fasciolosis among dairy farm personnel in the Eastern Cape Province, South Africa. A higher proportion of respondents from coastal regions were knowledgeable about the aetiology P equals 0.001 and the intermediate host P equals 0.000 of fasciolosis than those from the inland farms. A higher proportion of dairy farm personnel were not knowledgeable about the clinical signs of fasciolosis P equals 0.000 and the zoonotic potential P equals 0.001 of the disease. All farm personnel knew that season P 0.001 is a risk factor associated with snail-borne disease and occur throughout the year. However, the majority 63.2 percent of farm personnel agreed that fasciolosis was more prevalent in the summer followed by spring 27.6 percent, autumn 4.7 percent and winter 4.7 percent. Experiment 2 examined the body condition scores, fluke intensity, liver pathology, and carcass quality of various dairy cattle genotypes affected by Fasciola spp. infection, within three high-capacity abattoirs located in the Eastern Cape Province, South Africa. Milder infections were significantly noted to occur at a higher rate P 0.05 in Cross-bred cattle 46.9 percent followed by Holstein Friesian 39.5 percent and Jersey 30.9 percent cattle. Conversely, moderate infections were more prevalent P 0.05 among Jersey cattle 28.0 percent followed by Holstein Friesian 15.4 percent and Cross bred 1.9 percent cattle. Severe infections were more frequent P 0.05 in Holstein-Friesian 45.1 percent, followed by Jersey 41.9 percent and then Cross-bred 31.7 percent cattle. Heavy infections were observed in cattle with poor body condition scores in CA3. Mild infections were observed more in cattle with moderate body condition scores in CA3 and CA1 and less in CA2. Chapter 5 compared the detection rate of Fasciola infections using four different methods real-time quantitative polymerase chain reaction qPCR faecal sedimentation, antibody enzyme linked immunosorbent assay Ab ELISA and post-mortem liver examination among naturally infected cattle slaughtered at the abattoirs in the Eastern Cape Province, South Africa. A total of 145 slaughter cattle were screened for Fasciola infestation by liver examination, and corresponding samples from individual animals were analysed in the laboratory using Ab ELISA faecal sedimentation, and qPCR. The detection rate by liver inspection gold standard was significantly P 0.0001 higher than that by sedimentation and Ab ELISA but not significantly P 0.05 different from that by qPCR P equals 0.198. Post-mortem liver examination 94.5 percent and qPCR 90.4 percent had higher detection rate compared to Ab ELISA 22.6 percent and sedimentation 3.53 percent, respectively. The agreement between Ab ELISA and sedimentation was poor to slight kappa equals to minus 0.09 and with qPCR it was 0.008. The fourth experiment investigated genomic sequence of Fasciola spp. from faecal sample of slaughtered dairy cattle in Eastern Cape Province South Africa. Sequential and phylogenetic analyses were conducted to detect the presence of Fasciola spp. from the faecal samples obtained at the high throughput abattoirs. Fasciola hepatica and F. gigantica ITS 2 sequences were obtained by amplifying the 364 bp and 300 bp genes, respectively using species specific conventional PCR assays followed by cloning and sequencing. The phylogenetic tree revealed the presence of Fasciola hepatica as the only aetiological agent infecting dairy cattle in the Eastern Cape Province South Africa. The study concluded that dairy farm personnel lacks knowledge about zoonotic potentials, aetiological agents of fasciolosis high fluke intensity and its impact on carcasses sensitivity and suitability of detection methods. The study observed Fasciola hepatica as the only trematode spp. present in Eastern Cape Province South Africa.Thesis (PhD) -- Faculty of Science and Agriculture, 202
“Rwanda cannot be exorcised”: representations of the trauma of the Rwandan Genocide in selected films and novels
Although the Rwandan genocide (itsembabwoko in Kinyarwanda) has often been compared with the Holocaust, in terms of literary and cinematic narratives it has been largely underrepresented, with the notable exception of the release of the films Hotel Rwanda (2004) and Sometimes in April (2005), as well as novels such as Gil Courtemanche’s A Sunday at the Pool in Kigali (2003). However, although there is now a larger oeuvre of works on the subject, they are often not widely known or disseminated beyond their countries of origin. Of even greater concern is the fact that most cinematic narratives on itsembabwoko rely on Western narrative structures in their approach to storytelling. As a result, trauma in these narratives largely tends to focus on the experiences of Western protagonists or on Rwandan protagonists from a Western point of view. This tendency can be tied to the use of Western trauma theory in exploring the effects of the genocide on its witnesses and survivors, at the expense of arguably more relevant postcolonial trauma theory. This presents a problem in theorising the trauma of itsembabwoko, which occurred in a highly specific historical context involving the processes of colonization and decolonization, and in which the difficulties in unifying a population which had been split along socio-economic lines since pre-colonial times remained unresolved. Despite its shortcomings in the postcolonial African context, it would be a mistake to dismiss Yale trauma theory entirely, however, since theorists such as Cathy Caruth still provide valuable insights into the effects of trauma on both the individual and the collective. As a result, I have sought to find commonalities between the two schools of thought, so as to create a more nuanced view of itsembabwoko, its repercussions and the violence preceding it. In writing this thesis, I have selected mostly Rwandan authors, often survivors of the genocide, whose works provide an alternative view of Rwanda’s violent history to that presented in the works mentioned above. Given that the majority of the texts I focus on have been released more recently – mostly the mid-2010s – and are less well-known than their Western counterparts, they provide the opportunity to compare first-hand accounts with those that can only partially recreate the terror of anti-Tutsi violence in Rwanda. My analysis hopefully provides a new perspective on the dominant narratives which have shaped the way in which non-Rwandan (predominantly Western) audiences understand the genocide. The overall aim of this thesis, then, is to demonstrate the importance of placing the genocide and its resultant trauma in a broader historical context, with a view to establishing that it is shortsighted to ignore the impact of pre- and post-genocide trauma on the Rwandan people when discussing itsembabwoko. Though this has been achieved in socio-historical studies, such as Mahmood Mamdani’s When Victims Become Killers: Colonialism, Nativism, and the Genocide in Rwanda, very little has been produced on literary and cinematic representations of the genocide.Thesis (MA) -- Faculty of Humanities, Literary Studies in English 202
The experiences of TVET College students in learning English as a second language
The aim of the study was to investigate how the students experience English language learning at a Technical Vocational Education and Training College (TVET) in Eastern Cape. Most of the students enrolled at TVET colleges come from lower socioeconomic backgrounds and speak English as a second language. Eight participants were purposively selected from an NCV class in one campus of the college representing the Engineering studies. This was done using the non-probability purposive sampling. The study employed a qualitative design with drawings drawn by the participants to reflect on how they learnt English in schools. The drawings were discussed in a focus group. Video diaries were also used to investigate how students experience English learning in a TVET College. Data were analysed thematically whereby information was categorised into themes that emerged from the responses of the participants. The findings revealed that students had difficulties in learning English language basic skills from lower grades. They did not master the basic language skills and that negatively affected their ability to use language for communication. They were admitted to college with limited vocabulary, and they had no confidence in using the language The study recommends that an organised and effective assessment of literacy skills be done through the pace test. The students who are found to have gaps in English be given an extra class to help them. Recommendations for further study are also provided.Thesis (MEd) -- Faculty of Education, School of Post Schooling, 202
Social ecosystem for skills research inclusivity, relationality and informality
Eighty per cent of Africans work in the informal economy. In this chapter, we consider the highly informal, unregulated and often marginalized contexts that form the majority experience of living, working and learning. Situating the praxis of horizontal learning within these very normal contexts of informality demands renewed analysis into the questions of how horizontal learning is facilitated, by whom, with what resources, and why. Following on from Chapter 4, we develop our approach to social ecosystems further through two empirical case studies offering distinct lenses on to the informal sector. In Gulu, we consider the current dynamics of learning and inclusion among informal traders at a local market and in a set of food and clothing initiatives; in Alice, we reflect on an intentional effort on behalf of established, formal institutions to explore new approaches to teaching and learning through support of expansive informal learning in the context of food growing. While our focus across the book is on the range of labour markets and livelihood opportunities, it is appropriate to start our empirical chapters by focusing on the labour market of the majority
Labour law and non-standard employment: case studies of selected workplaces in Makhanda
While precarious and poorly-regulated forms of employment are on the increase worldwide, the patterns vary significantly over time and space. This study sought to understand employees’ perceptions of non-standard employment (NSE), the reasons why employers resort to NSE and why they use particular types of NSE. In addition, the research explored how labour law protections for NSE are applied in particular workplaces. Drawing on the regulation approach, which provides a conceptual framework to explain changes in production and employment relationships under specific spatio-temporal conditions, this study focused on three companies in Makhanda. Furthermore, this study employed a qualitative research method to explore the employees’ perceptions on non-standard work. This was done by using an interpretive approach to explore how workers experience NSE and how Eastern Cape citizens in Makhanda navigate the scourge of unemployment and exploitation in the labour market. Moreover, the research investigated how employees experience the efficacy of their employment rights in the workplace. The employment rights observed in the study include (among others) leave, contract of employment and collective bargaining; all of which are provisions in the Labour Relations Act. The findings in the study do validate the use of NSE as a ‘tool’ to curb high unemployment. However, the study finds that the positive result of job creation does not signify job security for non-standard workers, instead beneath the rhetoric of job creation and investment, lies hidden the exploitation of vulnerable groups of citizens and extension of precarious forms of employment. This is because businesses engage in economic competition locally and globally. Therefore, in order to successfully compete against other, employer’s cut on labour expenses by adopting a “race to the bottom,” the effect of which is paying employees below the minimum wage. Moreover, this study opposes the argument that NSE is a stepping stone to permanent employment. The conditions which prevail in NSE are distinctly different from those in the Standard Employment Relationship (SER), making it difficult for employees to transition to a new labour market. The latter is more aligned with the heterodox approach than it is to the orthodox approach to labour market.Thesis (MA) -- Faculty of Humanities, Sociology, 202
Knowledge, attitudes and practices regarding social media as a teaching strategy at a public nursing college in Limpopo province
The emergence of social media (SM) in the academic arena has demonstrated the potential for innovation in teaching and learning. Moreover, its appeal is enhanced by its accessibility and affordability. Consequently, the use of SM has increased globally and has influenced interaction and socialisation amongst educators and learners. In addition, SM has affected learning and how educators teach and collaborate with learners when constructing knowledge in higher education settings. However, despite its advantages, nurse educators' use of SM is less than optimal in teaching. Therefore, the study aimed to explore nurse educators' knowledge, attitudes and practices regarding the use of SM as a teaching strategy at a public nursing college in the province of Limpopo, South Africa. The research design employed for this study was quantitative, explorative, descriptive and contextual, and Roger’s diffusion of innovation theory underpinned the study. Stratified, proportionate sampling was used to select the nurse educators who were respondents to the study’s survey via a questionnaire as the data collection instrument. An educator as a specialist in the use of SM, a statistician and the study supervisors reviewed the questionnaire. A pilot study was conducted to check the validity and reliability of the data collection instrument. Data gathered through the survey was statistically analysed to produce descriptive statistics. The researcher adhered to the ethical principles of respect, beneficence, and justice. The research results indicated that the nurse educators in the study were knowledgeable about SM platforms. Moreover, although they had a positive attitude towards incorporating SM as a teaching strategy. Therefore, based on the research results, recommendations were to facilitate the use of SM as a teaching strategy.Thesis (Ma) -- Faculty of Health Science, 202
Recent progress in the conversion of agricultural waste into functional materials
Agricultural waste is enriched with a variety of environmentally friendly materials that can potentially boost economic growth, reduce the spread of contagious diseases, and serve as a carbon-free renewable energy bioresource. Biopolymers produced from agricultural waste have a range of applications in medicine, agriculture, pharmaceutics, and industrial factories. The chemical extraction of biopolymers from biomass requires a series of alternating alkali, acid, and alkali treatments at controlled temperatures. Chemical extraction of plant-based biopolymers requires elevated temperatures (70–100°C), while for animal and sea organism-based biopolymers, moderate temperatures of 25–60°C are used. The obtained biopolymers are functionalized into various materials for application in a wide range of industries. The reported functional materials are loaded with inorganic nanomaterials, plant extracts, and organic compounds, which resulted in a synergistic effect and enhanced activity of the materials. Several researchers have synthesized biopolymers with synthetic polymers to improve their bioavailability, tensile strength, shelf life, and UV adsorption. This review article reports the extraction techniques of biopolymers from agricultural waste and their application in wound healing, water treatment, food storage, passive cooling, and cosmetics. The dearth of scientific articles on the applications of biopolymers generated from agricultural waste produced from food crops grown in Africa is a motivation for the present compilation
An architecture for feedback-driven learning analytics
Feedback from students provides an opportunity to gain insights into students’ learning behaviour and participation in higher education learning and teaching. Academic staff are expected to constantly review and improve the learning and teaching environment where feedback contributes vital information toward pedagogical decision-making. Reviewing student feedback is essential but time-consuming, making it crucial to explore more effective and efficient ways to analyse and present feedback that encourages intervention and support. The general design science research evaluation pattern guided the construction and evaluation of a Feedback-Driven Architecture (FDA) for Learning Analytics (LA) to address this problem. An FDA implementation using a suitable case demonstrated each component validating the feasibility and effectiveness of the proposed design. The components identified for inclusion in the FDA were integrated within layers of a three-tiered architecture pattern. The data layer incorporates the collection, preparation and storage of learning management system data which includes free-form narrative student feedback. The application layer contains logic to support the analysis of free-form narrative student feedback and extraction of learning analytics. Arrangement and visualisation of information in the presentation layer aim to promote appropriate intervention and support. Evaluation using a case study showed that the FDA provided necessary guidelines for developing an implementation that produced feedbackdriven learning analytics. Data preparation transformed unstructured data into a suitable representation for effective analysis. Cohorts within narrative feedback responses were identified using the k-means clustering algorithm and latent Dirichlet allocation. Logistic regression and support vector machines were applied as predictive models and trained using extracted quantitative markers to predict academic success.Thesis (MSc) -- Faculty of Science, School of Computer Science, Mathematics, Physics and Statistics, 202
Functional traits and potential physical drivers of offshore benthic epifauna on the west and south coast of South Africa
Offshore benthic ecosystems (> 200 m depth) are challenging to access and remain poorly studied globally. Epifaunal invertebrates comprise a critical trophic level in benthic ecosystems and can serve as indicators of the overall health and functioning. There is limited knowledge of South Africa’s offshore marine environment and benthic ecosystem functioning due to limitations in funding, resources, and the lack of suitable science-based monitoring tools which are vital for sustainable management into the future. The aim of this research was to improve the understanding of epifaunal functioning as well as their potential physical drivers in 13 South African offshore biogeographic ecotypes. The objectives of this study were threefold: 1) to investigate the functional composition of benthic epifauna from 13 biogeographic ecotypes, 2) to quantify the Functional Diversity (FD) associated with epifaunal traits between the different assemblages (i.e. biogeographic ecotypes), and 3) to evaluate the relationship between physical variables and epifaunal functional traits at the biogeographic ecotype level. Biological trait-based approaches were applied to a subset of 80 benthic epifaunal species collected from 909 offshore stations along the west and south coasts of South Africa. Nine biological traits (associated with life history, morphology and behavioural characteristics exhibited by each species) were classified into 39 modalities and weighted with biomass. Community Weighted Means (CWMs) were used to evaluate the benthic epifaunal functional trait composition of the 13 biogeographic ecotypes. Functional composition across the 13 ecotype assemblages on the west and south coast appeared to be dominated by species with similar trait compositions, such as large, long-lived, surface crawling/burrowing/filter-feeding epifauna with medium to no mobility, however, their percentage of contribution to trait expression (CWMs) was higher on the west coast. Functional diversity indices (alpha and beta) showed higher overall FD for west coast ecotypes, with most ecotypes on the continental shelf (except slopes and canyons) being significantly different (p0.05). The fourth-corner method was used to test the significance of individual traitenvironment relationships. The fourth-corner results were similar overall to the RLQ analysis, revealing that three physical variables (temperature, depth, and fluorescence) were significantly correlated to two trait modalities belonging to the feeding mode filter-feeders (FM1) and scavengers (FM5). Scavengers were positively correlated with depth and negatively with temperature, while filter-feeders were positively correlated with fluorescence levels. This indicated that the presence of scavenging epifauna increased with a decrease in temperature and an increase in depth. While a positive significant correlation between filter-feeders and fluorescence suggested their potential tolerance or preference for environmental conditions with high levels of fluorescence. This research was the first such study to explore the functional composition and diversity of benthic epifauna and their relationship with potential physical drivers in offshore west and south coast benthic biogeographic ecotypes of South Africa. The application of new tools to quantify the functional diversity of epifauna as indicators of ecosystem health, and their potential physical drivers. This provided a platform on which to advance our understanding of benthic communities and the roles they play in ecosystem functioning under changing environments. Functional trait-based approaches such as those applied in this study can provide us with vital information on the relationship between biodiversity, ecosystem functioning, and physical drivers. Environmental stressors and changing climate patterns threaten to impact marine ecosystems and their functioning. Benthic epifauna are especially sensitive to changes in their environment and these fluctuations could potentially lead to the loss of certain benthic functionality, altering the thresholds these ecosystems have to response to disturbances. This undermines the stability of these ecosystems which can have ripple effects on the health of these ecosystems and their ability to provide the ecosystem services humans dependent on. Having suitable tools to track current and predict future changes will therefore be vital to inform management and conservation strategies for sustainable ocean utilisation.Thesis (MSc) -- Faculty of Science, School of Environmental Sciences, 202
From euphoria to disenchantment: the intangible in Black post-apartheid South African fiction
Access restricted. Exptected release date 2025.Thesis (PhD) -- Faculty of Humanities, Literary Studies in English, 202