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The effect of work ethic and employee well-being on turnover intention and job performance in a power utility organisation in the Eastern Cape
No full textLoss of highly skilled and experienced employees and associated turnover is of significant concern at the power utility organisation. The aim of the study was to investigate the effect of work ethic and employee well-being on turnover intention and job performance in this organisation. A positivist, quantitative study with a survey research design was used to conduct the study. A convenience sampling method and an online questionnaire were utilised to collect data. Responses were received from 106 participants. SPSS version 28 and PLS-SEM version 4 were used for data analysis. A path coefficient of -.467 revealed a significant negative effect of employee well-being on turnover intention, indicating that improving employee well-being predicts a decline in turnover intention. A path coefficient of -.016 indicates a negative nonsignificant impact of work ethic on turnover intention, indicating that while an increase in work ethic reduces turnover intention, the effect is not statistically significant. A path coefficient of .256 indicates a significant positive effect of employee well-being, indicating that an increase in employee well-being significantly increases job performance. A path coefficient of .168 indicated that work ethic significantly and positively affects job performance, indicating that a rise in work ethic is associated with a rise in job performance. A path coefficient of -.213, showed that turnover intention has a significant negative effect on Job Performance, indicating that a rise in turnover intention is associated with a decline in job performance. Recommendations to help the organisation with turnover intention reduction strategies are presented.Thesis (MComm) -- Faculty of Management and Commerce, 202
Phytoplankton and aquatic macroinvertebrate assemblages from coastal and inland lakes of South Africa
Get PDFFreshwater lakes are generally defined as permanent natural standing water bodies, with some of them having a direct and indirect connection with groundwater, rivers, and the ocean. Freshwater lakes provide essential socio-economic and ecological goods and services including recreation, aesthetic, support aquatic biodiversity, food in a form of fisheries and water for domestic use. Given their critical role in sustainability in providing socio-economic services, freshwater lakes are among the most threatened ecosystems globally due to intense human impacts over the last decades. South Africa has limited freshwater lakes, which according to the National Biodiversity Assessment (NBA 2018), we know little about their current biology except historic aquatic biodiversity studies conducted in the early 1940s. There are no management strategies in place to protect and conserve freshwater lake biodiversity and important ecosystem services. This thesis aims to: (1) produce a biodiversity inventory of phytoplankton and aquatic macroinvertebrate species and, (2) investigate important environmental drivers responsible for phytoplankton and aquatic macroinvertebrate species composition from six South African freshwater lakes. It was hypothesized that the three lake types will show different phytoplankton and aquatic macroinvertebrates species composition attributed by the geographical region (coastal and inland lake) and related physico-chemical parameters. Study sites consisted of two Northern KwaZulu-Natal coastal lakes (hereafter Coastal Lakes, CL) i.e., Lake Sibaya, Lake Mzingazi; two fresh inland lakes (hereafter Fresh Inland Lakes, FIL) i.e., Lake Banagher fresh and Lake Tevrede Se Pan; and two inland salt lakes (hereafter Salt Inland Lakes, SIL) i.e., Lake Banagher salt and Lake Chrissiesmeer, all inland lakes are situated in Mpumalanga province, together with other Pans making up the Mpumalanga Lake District of South Africa. The study sites were categorized based on their geographical position i.e., coastal vs inland and physico-chemical characteristics i.e., the presence and absence of aquatic vegetation, dominate substrate, salinity and different physico-chemical concentration i.e., Temperature, Dissolved Oxygen. Aquatic macroinvertebrates were collected from four littoral zone sites ( 5-meters depth) and (0.5-meter depth) from the water surface at each lake during summer and winter season. The results were consistent with our hypothesis that both phytoplankton and aquatic macroinvertebrate species composition were influenced by physico-chemical parameters and that the differences in salinity concentration and aquatic vegetation between CL, FIL, and SIL were the driving factors for phytoplankton and aquatic macroinvertebrate species composition. In summary, one hundred and twenty-two phytoplankton taxa were collected and identified during this study, belonging to seven Phyla which included Chlorophyta, Bacillariophyta, Cyanophyta, Chrysophyta, Dinophyta, Euglenophyta, and Cryptophyta. The most abundant phytoplankton groups were Bacillariophyta and Chlorophyta. Phytoplankton relative taxa abundance, Pielou’s evenness, taxa richness, and Shannon diversity were significantly different between lake types. Aquatic macroinvertebrates, on the other hand, summed up to 10 orders, 67 families, and 80 taxa. The most abundant group were the order Coleoptera, Hemiptera, Odonata, and Gastropoda. Aquatic macroinvertebrate relative taxa abundance, taxa richness, and Shannon diversity were also significantly different between lake types. Aquatic macroinvertebrate relative taxa abundance, Pielous evenness, and Shannon diversity index were not significant between seasons, and only taxa richness was significant. Canonical analysis of principal coordinates (CAP) results further showed unique and distinct phytoplankton and aquatic macroinvertebrates community composition between lake types. The present study provides baseline biodiversity inventory (or species list) for important lake ecosystems biological indicators i.e., phytoplankton and aquatic macroinvertebrates and species composition in relation to lake type for six freshwater lakes in South Africa. Furthermore, the study provides empirical evidence that will inform policy and the development of management strategies for freshwater lakes in South Africa which is currently missing. The current study will also contribute to the next National Biodiversity Assessment Report (2024), concerning the freshwater lakes biological data deficiency noted in the previous NBA (2018) report. The study will also fill up the gaps to better understand species composition in lake systems and how they function which is currently limited.Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 202
Mega-churches and the neo-Pentecostalisation of South Africa’s black middle class
Get PDFThis thesis argues that the presence and expansion of South African neo-Pentecostal Charismatic Churches (neo-PCCs) and mega-churches holds significant political economy consequences. Methodologically, the thesis is anchored on digital and in-person ethnography, life history and in-depth interviews. The central argument of this thesis is that neo-PCCs and mega-churches are holding spaces for societal change. The symbolic capital of neo-PCCs and mega-churches and the neo-Pentecostalised habitus, which is co-produced by middle-classes acts as a conduit that facilitates social transitions of political and economic orders. Indeed, religion emerges as a transition mechanism as Durkheim argued for France. It has helped South African social groups to extricate themselves from racist discourses, foster non-racialism and build empowered, somewhat deracialised modern middle-class discourses and tastes. White and Black middle classes have co-produced neo-Pentecostal habiti in the post-apartheid era, that have first, built dispositions for neoliberal capital democracy from apartheid capitalism, second as a middle classing and elite making mechanism and field, third as a stabilising, consolidating and upward strategy of social reproduction. In this way, neo-PCCs and the Black middle class have significantly affected the post-apartheid social formation by producing dispositions that uphold financialised neoliberal capitalism. Significantly, accumulated cultural capital is an indispensable resource in initiating and building post-apartheid institutions. As in the neo-PCC field, it has been pastors, prophets and bishops that have demonstrated the capability to accumulate, transubstantiate and maintain cultural capital that has made their churches comparatively durable social institutions. In a political economy context of state-capture and corruption, a post-GFC-and-COVID-19 milieu characterised by the absence of economic growth, rising unemployment, business closure and ever-increasing interest rates that affect indebted middle-class households and the poor alike, the mega-churches studied herein and their neo-Pentecostalised Black middle class adherents expressed an intense dislike for South African politicians across party lines, and especially the poor performance of the African National Congress-dominated state. So that it is possible that mega-churches and their leaders will outlive many new political parties and independents in the same way that they have outlived post-1994 political parties such as the New National Party, Agang South Africa and the Independent Democrats. Much like the buffer Black middle class that was promoted by the apartheid state as a project of reforming apartheid in the 1970s, whose political activism was pragramatically disengaged, this will likely continue, unless if, generally the post-apartheid Black middle classes shift their sociality from in-ward looking-enclaved social anxiety.Thesis (PhD) -- Faculty of Humanities, Sociology, 202
Enhancement of the electrocatalytic activity of phthalocyanines through the reduction in symmetry and conjugation to detonation nanodiamonds
Get PDFThis thesis reports on the synthesis of novel phthalocynines tetrakis[(benzo[d]thiazol-2ylthio)phthalocyaninato]cobalt(II)chloride (complex 1) and tris(2-(ethylthio)benzo[d]thiazole)2-(phthalocyanine-9-ylthio)propionate cobalt(II) chloride (complex 2). The complexes are combined with DNDs via different techniques such as π-π stacking, covalent linkage and sequential modification on glassy carbon electrode. The synthesized MPcs and conjugates were characterized using UV-visible, mass, Fourier transform infrared, and Raman spectroscopies as well as transmission electron microscopy and dynamic light scattering. Combining MPcs with DNDs sought to improve electrooxidation of hydrazine. The electrochemical studies were conducted using cyclic voltammetry, chronocoloumetry, electrochemical impedance spectroscopy and chronoamperometry. Hydrazine was utilized as an analyte of interest, due to its mutagenic and carcinogenic effects. Glassy carbon electrodes (GCE) were modified using drop and dry method. The conjugation via covalent linkage proved to be the best way of enhancing electrocatalytic properties. Since it performed better in terms of limit of detection (0.33 μM), even though catalytic rate and sensitivity are not the highest.Thesis (MSc) -- Faculty of Science, Chemistry, 202
Comparison of the metabolic physiology of exploited and unexploited populations of red roman (Chrysoblephus laticeps) along the south coast of South Africa
Get PDFAnthropogenic-induced climate change and exploitation pose threat to many marine fishes on which a vast majority of people around the world depend. Rapid changes in sea surface temperature have a direct impact on the physiology of ectothermic organisms such as fish, potentially resulting in changes to population distribution, abundance, and demographics. In the face of climate change, the impacts of increasing temperature variability on fish populations may be exacerbated by exploitation. Understanding how the resilience of exploited populations is affected by climate change is critical to predict how fishes will respond in the future. This study aimed to augment our knowledge on the impact of exploitation and thermal variability on fishes by comparing the thermal physiology of an exploited and unexploited population of the resident, reef-dwelling, Chrysoblephus laticeps. Twenty live fish were collected from the exploited, Cape St Francis and 18 fish from the unexploited, Goukamma Marine Protected Area and transported to the laboratory. The metabolic performance, in terms of standard metabolic rate (SMR), maximum metabolic rate (MMR) and aerobic scope (AS) of individual C. laticeps were estimated repeatedly at 10 ℃, 16 °C and 21 °C. Linear mixed effects models were used to examine the relationship between temperature, population, and metabolic rate and a ‘cvequality’ test analysis was used to compare the variance structure of the metabolic rate regression model for each population. Overall, the findings of this study show that Chrysoblephus laticeps from the unexploited population maintains a significantly higher aerobic scope (AS) across all temperature treatments (10, 16 and 21 ℃) when compared with those from the exploited population. In addition, the maximum metabolic rate (MMR) of individuals from the unexploited population was significantly higher than that of individuals from the exploited population, but there was no evidence to suggest that variability was significantly different between the populations. On the other hand, the individuals from an exploited population had a significantly higher standard metabolic rate (SMR) at high temperatures of 21 ℃, while the unexploited population had a low SMR at these high temperatures, but a high SMR at 10 ℃. Despite these differences there was no significant variation in the SMR between the two populations. The findings of this study confirm previous work on different exploited and unexploited populations of C. laticeps and together these findings suggest that hook and line exploitation lead to reduced physiological phenotypic diversity and reduced physiological performance in exploited fish populations. These findings emphasise the importance of incorporating the iii physiological information to develop viable fisheries management tools in the context of climate change. This study also highlights the effectiveness of MPAs in conserving highperformance physiological phenotypes to maintain phenotypic diversity in fish populations. Future research should aim to evaluate the efficacy of existing MPAs in preserving the physiological diversity of important hook and line fisheries species, while fisheries managers should consider augmenting their approaches through the incorporation of well-designed MPA’s to promote physiological diversity. This will be critical to advance the development of sustainable management practices, not only in a South African context but globally, where oceanic and coastal environmental conditions are expected to rapidly change in the future.Thesis (MSc) -- Faculty of Science, Ichthyology and Fisheries Science, 202
Computational studies in human African trypanosomiasis
Get PDFHuman African trypanosomiasis (HAT) is a neglected tropical disease (NTD) caused by two subspecies of the parasite, namely Trypanosoma brucei (Tb) gambiense (g-HAT) and rhodesiense (r-HAT). HAT is endemic in sub-Saharan countries, where the parasite transmission vectors, tsetse flies, breed. An estimated 70 million people remain at risk of contracting the disease, where the infection is classified as acute or chronic for g-HAT and r-HAT, respectively, with both forms ending in fatal meningoencephalitis when left untreated. Both g-HAT and r-HAT are responsible for widespread fatal epidemics throughout sub-Saharan African history, resulting from the complex molecular interplay between trypanosomes and humans through unique, innate immunity evasion mechanisms. Of interest, the Tbr subspecies expresses a serum resistance-associated protein (SRA), which binds to human serum lytic factor, apolipoprotein L1 (ApoL1), nullifying any trypanocidal activity. In response, ApoL1 (G1 and G2) variants found in humans of sub-Saharan African lineage have been cited for conferring resistance to the r-HAT infection in an interaction that is not fully elucidated In the event of successful infection, current HAT chemotherapeutics are plagued with complexity of administration, poor efficacy, toxicity, and potential drug resistance, highlighting a need for improved approaches. The parasite folate pathway provides a strategic target for alternative anti-trypanosomal drug development as trypanosomatids are folate auxotrophs, requiring host folate for growth and survival. Validated drug targets pteridine reductase (TbPTR1) and dihydrofolate reductase (TbDHFR) are essential for salvaging cofactors folate and folate biopterin crucial to parasite survival, making them viable targets for anti-folate investigation. The overall aims of this thesis were to a) provide insights into the molecular and dynamic basis of the SRA and ApoL1 interplay in HAT infection and b) identify safer and more efficient anti-folate anti-trypanosomal drug alternatives through in silico approaches. To achieve our first aim, in silico structure prediction was applied to generate 3D models of ApoL1 C-terminal variants G0, G1, G1G/M, G2 and G1G2, and four SRA variants retrieved from the NCBI database. The SRA and ApoL1 structures were inspected dynamically to identify the effect of the variants through molecular dynamics (MD) simulations. Dynamic residue network (DRN) analysis of MD trajectories was fundamental in identifying residues playing a vital role in the intramolecular communication of both proteins in the presence of mutations. Protein-protein docking was then applied to calculate plausible SRA-ApoL1 C-terminal wild-type complex structures to further elucidate the nature of SRA-mediated infection. Through MD simulations, twelve SRA-ApoL1 dimeric structures were narrowed down from five to two energetically sound complexes. The two feasible SRA-ApoL1 complexes (1 and 2) exhibited favourable communication observed through DRN analysis, including the retaining key communication residues identified in prior monomer DRN calculations. ApoL1 C-terminal variants were additionally incorporated into SRA-ApoL1 complexes 1 and 2 for further complex dynamics analysis This investigation into the nature of SRA-ApoL1 binding resulted in five primary outcomes: 1) highlighting the intramolecular effects ApoL1 variants have on the stability of the protein, 2) the identification of crucial SRA and ApoL1 communication residues in both monomeric or dimeric form, 3) the isolation of feasible SRA-ApoL1 complexes determined through global and local structural analyses 4) identification of residues crucial to the complex formation and maintenance of SRA-ApoL1, overlapping with those identified in (1), and 5) the minimal dissociative role of the G1 mutations in the complex, but compounding effect of the G2 deletion mutation. Computational modelling and drug repurposing were employed to achieve the thesis's second aim as they drastically cut down the costs involved in drug discovery and provide a more time-efficient screening method through numerous drug candidates. Using high throughput virtual screening, a subset of 2089 approved DrugBank compounds were screened against TbPTR1. The outputs were filtered to 24 viable compounds in 54 binding poses using binding energy and molecular interactions. Through subsequent MD simulations of 200ns, thirteen potential hit compounds were identified. The resultant hit compounds were subjected to further blind docking against TbDHFR and molecular dynamics to identify compounds with the potential for dual inhibition. The filtered subset was also tested in in vitro single concentration and dose-response bioassays to assess inhibitory properties against Trypanosoma brucei, complementing in silico findings. Post-molecular dynamics, four compounds exhibited high stabilities and molecular interactions with both TbPTR1 and TbDHFR, with two presenting favourable results in the in vitro assays. Three compounds additionally shared common structural moieties. In all, the in silico repurposing highlighted drugs characterised by favourable interactions and stabilities in TbPTR1, thus providing (1) a framework for further studies investigating anti-folate HAT compounds and (2) modulatory scaffolds based on identified moieties that can be used for the design of safe anti-folate trypanosomal drugs.Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 202
Developing and exploring a career education programme for disadvantaged FET-phase learners in the northern areas of Gqeberha: a developmental-contextual approach
Get PDFCareer development theory and assessments in South Africa have often been unscientifically applied to traditionally disadvantaged individuals, with little consideration of the diverse contexts of indigenous groups in the country. Assessment measures were not suited to the needs of learners from resource-constrained environments, nor could learners from these communities access these due to the associated financial costs. Recent research developments (using more holistic approaches) suggest that career counselling in South Africa should move towards better contextualized approaches to understanding and informing career development. Follow-up research projects show the value of such approaches with youth. Postmodern career development theories and models recognise that career counselling and development should focus on supporting individuals in constructing their careers from their own experiences within their environments, particularly for learners from disadvantaged communities whose career decisions and career development are inextricably influenced by their environments. The present study therefore employed the Developmental-Contextual Framework (DCF) to explore the perspectives of FET-phase Life Orientation (LO) educators on the LO curriculum (LO CAPS) for careers and career choices; as well as contextual factors influencing the career development of disadvantaged learners in public schools. The overall aim of the study was to co-construct and explore a customised career education programme, alongside LO educators, and determine whether FET-phase learners from disadvantaged public schools could benefit from such a programme. The DCF emphasises the dynamic relationship between an individual and the environment and the influences thereof on career development. The study draws from previous South African studies with disadvantaged learners and students that acknowledged the applicability of the DCF in the design of career programmes. This framework acknowledges the embeddedness of the individual in their proximal and distal environments and the role of these environments in the career decisionmaking of learners. The study was conducted in disadvantaged secondary schools in the Northern Areas of Gqeberha in the Eastern Cape, with LO educators in the FET-phase and the LO subject advisor at District level. At the initial interview stage the sample consisted of 9 participants and reduced to 6 participants in the focus group stage. The study underlined the impact of various contextual factors on career decision-making and the inclusion of these in career education programmes, particularly when working with learners from disadvantaged environments. Furthermore, the study illustrated that the career education of FET-phase learners in public high schools is lacking in many ways and that LO educators are not adequately trained to teach careers topics and therefore lack skills to support learners with their career development needs. The lack of, as well as poor and outdated career resources, impact negatively on the career education of learners in many public schools. Underpinned by Action Research, over a series of seven focus group meetings, an applicable and relevant programme of career-related activities based on the DCF was planned and evaluated by educators. The cumulative data were thematically analysed and the study revealed that the customised programme could potentially address the career development needs of learners from disadvantaged public schools, if educators are adequately trained to apply it. Recommendations are made for further piloting and implementation.Thesis (PhD) -- Faculty of Humanities, Psychology, 202
Visual representations of linear algebraic expressions: a case study in a Grade 9 after-school mathematics club
Get PDFVisualisation is commonly used as a tool in introducing algebra through visual or kinaesthetic sequences designed to prompt learners' development of a general rule for moving from a term's position to its output value. Fluency in both the concepts and the conventions of elementary algebra are essential to learners, as algebra forms the language in which many advanced mathematical ideas are encoded. Moreover, algebraic fluency is often associated with an ability to think abstractly about arithmetic processes. In many classrooms, however, research has shown that learners often focus on fluency in algebraic conventions rather than concepts, learning how to manipulate expressions without understanding the algorithms they are taught to follow. This trend can be linked to several causes, including teacher-centred mathematics classrooms in which learners are – whether implicitly or explicitly – encouraged to copy formulae and methods in order to ‘get it right in a test’ without necessarily grasping the underlying logical relationships. This case study, therefore, aimed to determine whether there was value in using visual, kinaesthetic models to broaden and deepen learners' use of algebra. To that end, in the context of an extra-curricular mathematics club that aimed to decentre the teachers and demand innovative ideas of the participants, six pairs of Grade 9 learners were tasked with creating visual representations of a linear algebraic expression using coloured building cubes. The responses to this task over the course of five assignments were many and varied and almost universally displayed a sustained internal logic that the learners were able to explain and develop. Most pairs began with a visual list of terms arranged in sets of towers, pyramids or, in one case, a spiral. At the end of the study, all but one of the pairs had settled on a Visual Expression, in which various colours were used to represent elements of the algebra such as the values of the coefficient, the variable and the constant term. The participants' representations grew in complexity over the course of the study and the conformity of the final responses showed that the club was a collaborative space in which learners shared ideas. However, the structure of the Visual Expressions and their own confessions of nerves about ‘getting it wrong’ in the interviews suggest that the participants were stuck in a mindset that led them to seek out and idealise the representation closest to the original algebra, even though that representation revealed little about the structural relationship underlying the expression.Thesis (MEd) -- Faculty of Education, Secondary and Post-School Education, 202
Governing pregnancy in the Global South: the case of post-apartheid South Africa
No full textWomen who sell sex (WSS) are vulnerable to violence. We present a scoping review of the last decade of research on the prevalence and incidence of, factors associated with, and services regarding violence against WSS in Eastern and Southern African (ESA). A systematic search of various databases resulted in 20 papers being reviewed. Inclusion criteria, applied by the first two authors, were as follows: empirical papers, key research problem is violence against WSS, and conducted in ESA countries. The lifetime prevalence of violence revealed in the studies ranged from 21% to 82%. A pattern of generalized violence against WSS from paying clients, male partners, strangers, family members, friends/acquaintances, and the authorities emerged. Factors associated with violence included the context within which the sex work occurs, alcohol use, type of sex exchange interactions, and personal factors (low education, low income, marriage, youth, high client volume, time in sex work, forced sexual debut, and internalized sex work stigma). WSS seldom access services after violence. Evaluations of two programs, a woman-focused HIV intervention, and the Diagonal Interventions to Fast-Forward Reproductive Health project, showed improvements in gender-based violence services. Findings suggest that targeted programmes should be paired with improving general health services and focus on promoting collective agency among WSS