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Changing the Way Providers Order Blood Return to Intensive Care Patients
No full textPatients with anemia and other conditions resulting in loss of blood often come with orders to return wasted blood to them. While using typical blood draw practices, patients are placed at an increased risk of infection when their blood is exposed to the outside world. My question is, will using a closed system decrease the instances of infection in patients who receive their waste blood after draws? By utilizing a waste syringe attached to the IV line, nurses can avoid detaching and reattaching syringes to collect and give back the patient\u27s blood. Patients who are already at an increased risk of infection due to being in the hospital will have a better outcome when their blood is not exposed to open air
LU-167.044a, Student Building (French)
No full texthttps://digitalcommons.longwood.edu/postcard/1050/thumbnail.jp
LU-167.001b, Artistic Panoramic of the Rotunda, East and West Wings
No full texthttps://digitalcommons.longwood.edu/postcard/1001/thumbnail.jp
LU-167.048a, Architectural drawing of the swimming pool
No full texthttps://digitalcommons.longwood.edu/postcard/1058/thumbnail.jp
LU-167.047a, French Building
No full texthttps://digitalcommons.longwood.edu/postcard/1056/thumbnail.jp
Denaturation Station: The Effect of Guanidinium Chloride on Yeast Flocculation
No full textFlocculation is a phenomenon found in yeast such as Saccharomyces cerevisiae in which cells aggregate in response to stressful environmental conditions. This occurs as the result of the interaction of flocculin proteins on the plasma membrane with mannose residues on the surface of other yeast cells which is often exploited in the brewing process to avoid expensive filtration steps. Guanidine is a chaotropic agent which reduces the stability of proteins by weakening the hydrophobic effect. Since it is believed that flocculation is dependent on protein-mannose binding interactions, if the structure of the flocculin proteins is altered by guanidine, their functionality would be impacted, causing a decrease in flocculation. The goal of this research is to quantify the impact of guanidinium chloride treatment at concentrations of 1M, 3M, and 6M on percent flocculation in the WLP002 strain of brewing yeast. Higher concentrations were hypothesized to invoke a greater decrease in percent flocculation. A modified version of Helm’s assay was performed, and percent flocculation was assessed using OD660 values