Institute of Hydrobiology, Chinese Academy Of Sciences
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NLRC3-like 1 inhibits NOD1-RIPK2 pathway via targeting RIPK2
No full textBoth NLRC3 and NOD1 belong to regulatory NLR subfamily based on their best-characterized function. In mammals, NLRC3 was reported to function by attenuating signaling cascades initiated by other families of PRRs. In teleosts, multiple NLRC3-like genes were identified through transcriptome sequencing. However, the functions of many NLRC3-like genes, especially the fish-specific NLRC3-like genes, remain unclear. In the present study, we report the functional characterization of a novel category of NLRC3-like proteins (named as NLRC3-like 1) from the zebrafish, which consists of a fish-specific FISNA, a conserved NACHT and five C-terminal LRRs domains. The expression of zebrafish NLRC3-like 1 was inducible in response to Edwardsiella piscicida infection. During bacterial infection, the in vitro and in vivo studies revealed that zebrafish NLRC3-like 1 overexpression facilitated bacterial growth and dissemination, together with the decreased survival rate of zebrafish larvae infected with E. piscicida. The attenuated response by zebrafish NLRC3-like 1 in response to bacterial infection were characterized by the impaired expression of antibacterial genes, proinflammatory cytokines and Nox genes. Furthermore, zebrafish NLRC3-like 1 interacted with the adaptor protein RIPK2 of NODs signaling via the FISNA (Fish-specific NACHT associated domain) and NACHT domains. However, the interaction between zebrafish NLRC3-like 1 and RIPK2 inhibited the assembly of the NOD1-RIPK2 complex. Importantly, zebrafish NLRC3-like 1 inhibited NOD1-mediated antibacterial activity, NF-kappa B and MAPK pathways and proinflammatory cytokine production. All together, these results firstly demonstrate that zebrafish NLRC3-like 1 inhibits NOD1-RIPK2 antibacterial pathway via targeting the adaptor protein RIPK2
Identifying difference in primordial germ cells between XX female and XY male yellow catfish embryos
No full textPrimordial germ cells (PGCs) are singled out from somatic cells very early during embryogenesis, then they migrate towards the genital ridge and differentiate into gametes through oogenesis or spermatogenesis. Labeling PGCs with Localized RNA expression (LRE) technique by fluorescent proteins has been widely applied among teleost species to study the germ cell development and gonad differentiation. In this study, we first cloned and characterized the 3' untranslated regions (3'UTRs) of nanos homolog 1-like (nos1l), dead end (dnd), and vasa in yellow catfish (Pelteobagrus fulvidraco), and then synthesized the GFP-nosll/dnd/vasa 3'UTR mRNAs. Each of these three 3'UTRs could label PGCs in yellow catfish embryos, of which, vasa 3'UTR exhibited the highest labeling efficiency. To identify the differences in PGCs at embryonic stage, XX all-female and XY all-male yellow catfish embryos were produced and injected with GFP-vasa 3'UTR mRNA. We observed the PGC migration route in these two monosex embryos from 24 hpf to 7 dpf, and found there was no difference between them. Besides, the PGC number was counted at 48 hpf, and the result showed that the average PGC number in XX females (11.3) was significantly larger than that in XY males (8.1). These findings provide an insight into the development of PGCs in yellow catfish embryos and the relationship between embryonic PGC number and the later gonad differentiation
Spatial pattern of soil erodibility factor (K) as affected by ecological restoration in a typical degraded watershed of central China
No full textLong-term ecological restoration of degraded habitats can alter their near-land surface characteristics and thus affect soil erodibility factor (K). In this study, we used geostatistics coupled with structural equation modeling (SEM) to investigate the spatial patterns of K and quantify its relationship to potential impact factors (spatial location, topography, plant, and soil characteristics) in a restored watershed in the Danjiangkou Reservoir region of central China. Results showed that the K values in this watershed ranged from 0.026 Mg h Mj(-1) mm(-1) to 0.057 Mg h Mj(-1) mm(-1) land generally increased with soil depth. Across the watershed, the K values in the surface soil (0-10 cm) were the highest in the mid-stream region, followed by the downstream and upstream regions. No obvious pattern in the relationship between K and the slope was found in either the 0-10 cm Of 10-30 cm soil layer. Among the four land-use types, the K values followed the rank of shrubland > terrace and slope farmland > woodland. SEM results indicated that the variation in Kin this restored watershed was closely related to soil texture and soil organic matter (SOM) content. The external environmental variables (spatial location, topography, and plant) significantly influenced indirectly soil erodibility through their effects on the intrinsic soil characteristics (particularly the SOM) at both depths (R-2 of 0.67 and 0.33, respectively). Although soil texture was shown to be an important direct-effect factor (with path coefficients of 0.72 and 0.90, respectively), its external interpretation was the weakest among all the direct-effect factors (R-2 of 0.12 and 0.02, respectively). These results showed that afforestation, increasing soil organic fertility, and reducing tillage should be promoted in the future. (C) 2020 Elsevier B.V. All rights reserved
Study on the co-effect of maifanite-based photocatalyst and humic acid in the photodegradation of organic pollutant
No full textIn this study, the co-effect of clay mineral-based photocatalyst and humic acid on the photodegradation of dye was revealed for the first time. The clay mineral-based photocatalyst, maifanite/g-C3N4, was prepared using the co-calcining method. The physical and chemical properties of the maifanite/g-C3N4 photocatalysts with various ratios were characterized by multiple characterization methods, including SEM, XPS, BET, UV-Vis, FTIR, contact angle, and XRD. The respective degradation experiment of humic acid and RhB was performed using maifanite/g-C3N4 photocatalysts. The degradation process of mixture solution of humic acid and RhB was measured using EEM and UV-vis. The result indicates that in the presence of humic acid, low ratio of maifanite/g-C3N4 inhibits the production of by-products derived from the interaction of humic acid and the degradation of RhB. However, high ratio of maifanite/g-C3N4 is not conducive to the degradation of RhB. The ratio of 1:3 for maifanite/g-C3N4 is optimal for the photodegradation of RhB in the presence of humic acid. This article provides a new perspective to develop the co-effect of clay mineral and humic acid in the photodegradation of organic pollutant
鱼类组蛋白核苷酸的多态性及其在杀鱼爱德华氏菌感染中的作用
No full text组蛋白是染色体核小体的重要组分,在调控染色质结构、基因转录、个体发育等不同生物学过程中起着重要作用。为了研究鱼类组蛋白基因是否存在核苷酸的多态性以及组蛋白核苷酸多态性是否会影响鱼类的抗病力,本实验以斑马鱼和草鱼为研究对象,通过PCR扩增克隆了组蛋白H2A的全长开放阅读框;利用过表达技术、菌落平板计数、感染存活分析以及荧光定量PCR技术,研究了斑马鱼和草鱼组蛋白H2A核苷酸多态性不同变异体在杀鱼爱德华氏菌感染中的作用。在本研究中,实验发现鱼类组蛋白H2A存在着丰富的核苷酸多态性。序列分析结果显示斑马鱼和草鱼组蛋白H2A核苷酸多态性的变异体核苷酸序列相似性为90%~100%;而两两H2A核苷酸多态性变异体氨基酸序列之间最多只有3个位点存在差异。通过体内和体外抗菌实验可知,斑马鱼和草鱼组蛋白H2A核苷酸的多态性显著影响H2A的抗菌活性。此外,筛选出的抗菌组蛋白H2A核苷酸多态性的变异体在斑马鱼体内的过表达,不仅具有免疫增强的作用,还能显著增强斑马鱼对杀鱼爱德华氏菌感染的抗病力。本研究为筛选具有抗病作用的组蛋白H2A免疫保护原奠定了重要基础
信号分子介导藻类细胞程序性死亡的研究进展
No full text藻类是水生态系统中的重要初级生产者,在物质转换和能量迁移过程中发挥重要作用。细胞程序性死亡(PCD)作为一种细胞自我调控的死亡模式,受到多种信号分子的控制。研究发现藻类细胞在遭受环境胁迫的情况下,在形态和生理上均表现出类PCD的特征,同时伴随着活性氧/一氧化氮/钙离子(ROS/NO/Ca~(2+))水平的变化。研究认为, ROS/NO/Ca~(2+)作为信号分子介导藻细胞内的caspase-like酶活性变化,从而触发藻细胞的类程序性死亡。然而,对信号分子是如何在环境胁迫下的藻类细胞中引发类PCD仍知之甚少。文章综述了信号分子ROS/NO/Ca~(2+)介导藻类类PCD的研究进展以及信号分子间的级联关系,并对今后类PCD在该领域待开展的研究进行了展望
江西柘林湖水华蓝藻--长孢藻(Dolichospermum)的形态多样性及其分子特征
No full text长孢藻是具有产多种蓝藻毒素及异味物质潜在能力的丝状异形胞蓝藻.为了探究长孢藻的生物学特性,我们从江西柘林湖分离24株长孢藻,经形态鉴定分成4个种类,分别为浮游长孢藻(Dolichospermum planctonicum)、近亲长孢藻(D.affine)、卷曲长孢藻(D.circinale)、螺旋长孢藻(D.spiroides).16S rRNA基因序列表明柘林湖长孢藻与日本等地区的长孢藻高度相似,但只有近亲长孢藻与日本近亲长孢藻在分子系统进化树上聚为一支.基于16S-23S rRNA之间高变异性的ITS序列分析,4种长孢藻的D1-D1螺旋结构相同,藻丝为直型的浮游长孢藻、近亲长孢藻与藻丝为弯型的卷曲长孢藻、螺旋长孢藻的Box-B、V3螺旋结构差异较大.通过对毒素和异味的分子生物学检测显示24株长孢藻均不产毒,但是4株浮游长孢藻及1株卷曲长孢藻含有土腥素合成基因.本研究不仅对柘林湖水华蓝藻的多样性以及潜在生态风险提供科学的基础资料,也在流域层面上为鄱阳湖的水生态系统研究和保护提供了一定的科学基础
江西柘林湖水华蓝藻--长孢藻(Dolichospermum)的形态多样性及其分子特征
No full text长孢藻是具有产多种蓝藻毒素及异味物质潜在能力的丝状异形胞蓝藻.为了探究长孢藻的生物学特性,我们从江西柘林湖分离24株长孢藻,经形态鉴定分成4个种类,分别为浮游长孢藻(Dolichospermum planctonicum)、近亲长孢藻(D.affine)、卷曲长孢藻(D.circinale)、螺旋长孢藻(D.spiroides).16S rRNA基因序列表明柘林湖长孢藻与日本等地区的长孢藻高度相似,但只有近亲长孢藻与日本近亲长孢藻在分子系统进化树上聚为一支.基于16S-23S rRNA之间高变异性的ITS序列分析,4种长孢藻的D1-D1螺旋结构相同,藻丝为直型的浮游长孢藻、近亲长孢藻与藻丝为弯型的卷曲长孢藻、螺旋长孢藻的Box-B、V3螺旋结构差异较大.通过对毒素和异味的分子生物学检测显示24株长孢藻均不产毒,但是4株浮游长孢藻及1株卷曲长孢藻含有土腥素合成基因.本研究不仅对柘林湖水华蓝藻的多样性以及潜在生态风险提供科学的基础资料,也在流域层面上为鄱阳湖的水生态系统研究和保护提供了一定的科学基础
鲥鯸淀鱼类群落特征与生物完整性评价
No full text鱼类生物完整性指数(F-IBI,fish-index of biotic integrity)被广泛应用于河湖健康评价,可为水域生态修复和保护提供重要科学依据。于2018年10月—2019年7月在白洋淀区的典型湖泊鲥鯸淀开展鱼类群落采样,结合鱼类生物完整性指数评价体系和ABC曲线(abundance-biomass comparison curves),综合评价鱼类群落组成特征和水域健康状况。共发现鱼类30种,隶属4目10科,其中鲤科鱼类物种数最多,共21种,占鱼类总物种数目的70%。F-IBI评价结果表明,在鲥鯸淀的采样站点中,鱼类生物完整性评为"好~优"等级共有3个点;评价为"好"等级共有3个点;评价为"一般~好"等级共有4个点;评价为"一般"等级共有5个点;评价为"差~一般"等级共有8个点;评价为"差"等级共有3个点;没有点位评价为"优"和"极差"等级。总体上,春季和秋季呈现"一般~好",夏季呈现"差~一般"。ABC曲线结果显示,季度性汇总、春季和夏季所得的ABC曲线展现出明显的交叉趋势,表明总体状态、春季和夏季均呈现中度干扰状态;秋季的数量优势度曲线位于生物量优势度曲线之上,表明该季节呈现严重干扰状态。与20世纪50—70年代相比,白洋淀区域鱼类生物完整性整体呈下降趋势。过度捕捞、水质污染和极端气候等是白洋淀鱼类群落变化的重要因素,建议在湖泊生态修复的同时,通过划定禁渔区和延长禁渔期等方式,加强对鱼类资源保护和可持续利用
基于环境DNA宏条形码的洱海鱼类多样性研究
No full text研究使用环境DNA宏条形码(eDNA metabarcoding)检测洱海鱼类多样性,探索适用于洱海鱼类多样性监测和保护的新方法。通过水样采集、过滤、eDNA提取、遗传标记扩增、测序与生物信息分析的环境DNA宏条形码标准化分析流程,从洱海16个采样点中获得可检测的9个采样点数据,共检测出17种鱼类,其中土著种5种、外来种12种;鲫(Carassius auratus)、鳙(Hypophthalmichthys nobilis)、麦穗鱼(Pseudorasbora parva)、泥鳅(Misgurnus anguillicaudatus)和食蚊鱼(Gambusia affinis)为优势种。研究结果表明虽然环境DNA宏条形码无法完全替代传统的鱼类监测方法,但作为一种新兴的生物多样性监测手段,其可用于快速检测洱海鱼类多样性及其空间分布