Institute of Hydrobiology, Chinese Academy Of Sciences
Not a member yet
20118 research outputs found
Sort by
Effects of Bacillus licheniformis on the growth, antioxidant capacity, intestinal barrier and disease resistance of grass carp (Ctenopharyngodon idella)
No full textTo study the effect of dietary supplementation of Bacillus licheniformis FA6 on the growth, survival and intestinal health of grass carp, we assessed the antioxidant capacity, intestinal barrier, expression levels of immune genes, and the resistance to Aeromonas hydrophila AH-1 infection. Experimental setup comprised three groups (90 specimens each; average initial weight = 16.5 g): the control group was fed the basal diet without B. licheniformis, the low-dose (LD) group was supplemented with B. licheniformis at the concentration of 1 x 10(5) cfu/g, and the high-dose (HD) group with 1 x 10(6) cfu/g. After 56 days of growth trial, the challenge test with A. hydrophila AH-1 was conducted for 14 days. The results revealed that the grass carp in LD group and HD group had significantly (p < 0.05) improved percent weight gain (PWG) and specific growth rate (SGR) parameters. Additionally, the antioxidant status was improved, which included increased superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) levels in the serum, and upregulated mRNA levels of antioxidant enzymes MnSOD and catalase (CAT) in the intestine. Meanwhile, B. licheniforrnis FA6 supplementation groups exhibited a decreased mRNA expression of proinflammatory cytokines (such as IL-1 beta and TNF-alpha) and increased the expression of anti-inflammatory cytokine IL-10. Histological (villi length was increased) and gene expression (qPCR: upregulated ZO-1, occludin, and claudin-c) analyses suggested improved functioning of the intestinal barrier. Post-challenge mortality rates in LD and HD groups were significantly lower (56.6% and 70% respectively) than in the control group (100%). Overall, these results indicated that dietary supplementation of B. licheniformis FA6 can improve growth, antioxidant capacity, intestinal barrier functions and disease resistance of grass carp
Function characterization and expression regulation of two different-sized 3 ' untranslated region-containing interferon genes from clone F of gibel carp Carassius auratus gibelio
No full textFish interferon (IFN)-mediated antiviral innate immunity is the first line of defense against virus invasion. In the present study, we identify two fish IFN genes (here tentatively named IFNa and IFNc) with different-sized 3' UTRs from clone F strain of gibel carp Carassius auratus gibelio. Carp IFNa has a relatively short 3'UTR without AU-rich elements (AREs) but IFNc has a long one with 9 AREs. Functionally, carp IFNa and IFNc display significantly antiviral potential to viral infection, likely through induction of downstream IFN-stimulated genes (ISGs). Both carp IFN genes are induced by viral infection, poly(I:C) treatment and IRF3/7, which are ascribed to the IFN-sensitive response elements (ISRE) within their promoters. Carp IFN genes are also induced by each other and by themselves, indicating existence of a positive feedback loop in fish IFN-mediated antiviral immune response. Comparative analyses of 3'UTR-mediated expression regulation at mRNA and protein levels show that the ARE-containing 3'UTR of carp IFNc rather than the short 3'UTR of carp IFNa promotes mRNA decay but instead results in high-level protein expression, indicating that 3'UTR of fish IFN mRNAs might be a potential factor for regulation of IFN-mediated antiviral immune response. Considering a fact that a given protein function is largely related to its protein level, these results suggest that both promoter and 3'UTR contribute to the transcription and translation of fish IFN genes, thus shaping their eventually antiviral potential
A rapid and reliable method for identifying genetic sex in obscure pufferfish (Takifugu obscures)
No full textObscure pufferfish (Takifugu obscurus) is an important aquaculture species in China, and sex control breeding is extremely attractive in this species because the testes of male pufferfish are more popular and expensive. However, the sex-specific marker remains unknown and reliable genetic sex identification method has not been established, which has hampered the development of monosexual aquaculture. In this study, the sex-linked amhr2 SNP site of obscure pufferfish was firstly identified by sequencing and high-resolution melting (HRM) analysis. The males exhibit heterozygous genotype (C/G), while females have homozygous genotype (C/C), which is identical to its closely-related species (T. rubripes, T. poecilonotus and T. pardalis). Then, two pairs of male-specific primers were designed based on the sex-specific SNP site and a high-throughput sexing method was subsequently developed with benefits including high specificity and superb sensitivity. Particularly, this method has greatly reduced analysis time and costs compared to the previously established HRM analysis. These results will be beneficial to develop the sex control breeding in obscure pufferfish.</p
Eutrophication and heavy metal pollution patterns in the water suppling lakes of China's south-to-north water diversion project
No full textThis study used non-supervised machine learning self-organizing maps (SOM) in conjunction with traditional multivariate statistical techniques (e.g., hierarchical cluster analysis, principle component analysis, Pearson's correlation analysis) to investigate spatio-temporal patterns of eutrophication and heavy metal pollution in the water supplying lakes (i.e., the Gao-Bao-Shaobo Lake, GBSL) of the eastern route of China's South-to-North Water Diversion Project (SNWDP-ER). A total of 28 water quality parameters were seasonally monitored at 33 sampling sites in the GBSL during 2016 to 2017 (i.e., 132 water samples were collected in four seasons). The results indicated that: 1) spatially, the western and south-western GBSL was relatively more eutrophic and polluted with heavy metals; and 2) temporally, the lakes suffered from high risks of heavy metal contamination in spring, but eutrophication in summer while water quality in winter was the best among the four seasons. Two main potential sources of pollution and transport routes were identified and discussed based on the pollution patterns. These findings contributed considerably to providing in-depth understanding of water pollution patterns, as well as potential pollution sources in the water-supplying region. Such understanding is crucial for developing pollution control and management strategies for this mega inter-basin water transfer project. (C) 2019 Elsevier B.V. All rights reserved
Gnrh3 Regulates PGC Proliferation and Sex Differentiation in Developing Zebrafish
No full textGonadotropin-releasing hormone (Gnrh) plays important roles in reproduction by stimulating luteinizing hormone release, and subsequently ovulation and sperm release, ultimately controlling reproduction in many species. Here we report on a new role for this decapeptide. Surprisingly, Gnrh3-null zebrafish generated by CRISPR/Cas9 exhibited a male-biased sex ratio. After the dome stage, the number of primordial germ cells (PGCs) in gnrh3(-/-) fish was lower than that in wildtype, an effect that was partially rescued by gnrh3 overexpression. A terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) analysis revealed no detectable apoptosis of PGCs in gnrh3(-/-) embryos. Proliferating PGCs could be detected in wild-type embryos, while there was no detectable signal in gnrh3(-/-) embryos. Compared with wild type, the phosphorylation of AKT was not significantly different in gnrh3(-/-) embryos, but the phosphorylation of ERK1/2 decreased significantly. Treatment with a Gnrh analog (Alarelin) induced ERK1/2 phosphorylation and increased PGC numbers in both wild-type and gnrh3(-/-) embryos, and this was blocked by the MEK inhibitor PD0325901. The relative expression of sox9a, amh, and cyp11b were significantly upregulated, while cyp19a1a was significantly downregulated at 18 days post-fertilization in gnrh3(-/-) zebrafish. Taken together, these results indicate that Gnrh3 plays an important role in early sex differentiation by regulating the proliferation of PGCs through a MAPK-dependent path.</p
Molecular phylogenetics and mitogenomics of three avian dicrocoeliids (Digenea: Dicrocoeliidae) and comparison with mammalian dicrocoeliids
No full textBackgroundThe Dicrocoeliidae are digenetic trematodes mostly parasitic in the bile ducts and gall bladder of various avian and mammalian hosts. Until recently their systematics was based on morphological data only. Due to the high morphological uniformity across multiple dicrocoeliid taxa and insufficient knowledge of relative systematic value of traditionally used morphological characters, their taxonomy has always been unstable. Therefore, DNA sequence data provide a critical independent source of characters for phylogenetic inference and improvement of the system.MethodsWe examined the phylogenetic affinities of three avian dicrocoeliids representing the genera Brachylecithum, Brachydistomum and Lyperosomum, using partial sequences of the nuclear large ribosomal subunit (28S) RNA gene. We also sequenced the complete or nearly complete mitogenomes of these three isolates and conducted a comparative mitogenomic analysis with the previously available mitogenomes from three mammalian dicrocoeliids (from 2 different genera) and examined the phylogenetic position of the family Dicrocoeliidae within the order Plagiorchiida based on concatenated nucleotide sequences of all mitochondrial genes (except trnG and trnE).ResultsCombined nucleotide diversity, Kimura-2-parameter distance, non-synonymous/synonymous substitutions ratio and average sequence identity analyses consistently demonstrated that cox1, cytb, nad1 and two rRNAs were the most conserved and atp6, nad5, nad3 and nad2 were the most variable genes across dicrocoeliid mitogenomes. Phylogenetic analyses based on mtDNA sequences did not support the close relatedness of the Paragonimidae and Dicrocoeliidae and suggested non-monophyly of the Gorgoderoidea as currently recognized.ConclusionsOur results show that fast-evolving mitochondrial genes atp6, nad5 and nad3 would be better markers than slow-evolving genes cox1 and nad1 for species discrimination and population level studies in the Dicrocoeliidae. Furthermore, the Dicrocoeliidae being outside of the clade containing other xiphidiatan trematodes suggests a need for the re-evaluation of the taxonomic content of the Xiphidiata.</p
Morphological trophic variation of the cyprinid Megalobrama pellegrini (Tchang, 1930) with different keratinized beaks from upper reaches of the Yangtze River, China
No full textMegalobrama pellegrini, a cyprinid endemic to the upper Yangtze River China, has thick keratinized beaks on the jaws and feeds on algae and molluscs. The size of keratinized beaks varies among different individuals. In the present study, two populations, from Longxihe (LXH) and Chishui (CSH) Rivers, were examined in order to explore the morphological variation of keratinized beaks, together with an analysis of intestine length and of stable isotopes. The results showed that the ratio of upper to lower keratinized beaks varied from 0.84 to 2.06. We defined the ratio 1.26 as thicker upper beak (TUB) form. In the LXH population, there were more TUB forms, while EB forms dominated in CSH population. Intestine length showed a negative relationship with the upper to lower beak ratio (Pearson correlation coefficient = -0.697, P < 0.01) indicating the possible adaptation of beak size to food type. In addition, analysis of stable isotope showed that these two populations differed in their food, and there was a positive relationship between trophic niche and size of beaks (R = 0.5530 and 0.4927, respectively). This further supported the suggestion that the beak size was possibly adapted to food type, with the TUB form to a higher trophic niche and the EB form to a lower trophic niche
PELP1 Suppression Inhibits Gastric Cancer Through Downregulation of c-Src-PI3K-ERK Pathway
No full textBackground: Proline-, glutamic acid-, and leucine-rich protein 1 (PELP1), a co-activator of estrogen receptors alpha, was confirmed to be directly associated with the oncogenic process of multiple cancers, especially hormone-dependent cancers. The purpose of our research was to explore the biological function, clinical significance, and therapeutic targeted value of PELP1 in gastric cancer (GC). Methods: The expression status of PELP1 in GC cell lines or tissues was analyzed through bioinformatics data mining. Thirty-six GC tissue chip was applied to demonstrate the results of bioinformatics data mining assayed by immunohistochemical method. The expression status of PELP1 in GC cell lines was also analyzed using western blot. Correlation analysis between PELP1 expression and clinicopathological parameter was performed. Kaplan-Meier survival analysis was applied to analyze the relationship between PELP1 expression and total survival time. Three pairs of siRNA were designed to silence the expression of PELP1 in GC. After PELP1 was silenced by siRNA or activated by saRNA, the growth, plate colony formation, migration and invasion ability of the GC cell or normal gastric epithelium cell line was tested in vitro. Cell cycle was tested by flow cytometry. Nude mice xenograft experiment was performed after PELP1 was silenced. The downstream molecular pathway regulated by PELP1 was explored. Molecular docking tool was applied to combine chlorpromazine with PELP1. The inhibitory effect of chlorpromazine in GC was assayed, then it was tested whether PELP1 was a therapeutic target of chlorpromazine in GC. Results: PELP1 expression was elevated in GC cell lines and clinical GC tissue samples. PELP1 silence by siRNA compromised the malignant traits of GC. PELP1 expression positively correlated with tumor invasion depth, lymph node metastasis, tissue grade, TNM stage, but had no correlation with patient age, sex, tumor size, and tumor numbers. Kaplan-Meier survival analysis revealed high PELP1 expression had a shorter survival period in GC patients after follow-up. Q-PCR and western blot revealed PELP1 suppression in GC decreased expression of the c-Src-PI3K-ERK pathway. It was also implied that chlorpromazine (CPZ) can inhibit the malignant traits of GC and downregulate the expression of PELP1. Conclusions: In a word, PELP1 is an oncogene in gastric cancer and c-Src-PI3K-ERK pathway activation may be responsible for its tumorigenesis, PELP1 may be a potential therapeutic target of chlorpromazine in GC.</p
Mitochondrial genomes and 28S rDNA contradict the proposed obsoletion of the order Tetraonchidea (Platyhelminthes: Monogenea)
No full textDue to the incongruence of morphology-based hypotheses and scarcity of molecular data, validity of the order Tetraonchidea remains contentious. The only complete mitogenome currently available for the entire order is that of Paratetraonchoides inermis (Tetraonchoididae). To study the phylogeny of Tetraonchidea from mitogenomic perspective, we sequenced the first mitogenome for the family Tetraonchidae: Tetraonchus monenteron (Tetraonchidea). To get a nuclear-data perspective, we also sequenced nuclear 28S rDNA gene of both species. The mitogenome of T. monenteron does not have high A + T content, nor tRNA pseudo-genes, both of which were unique features reported in P. inermis. However, T. monenteron exhibits a unique gene order, with a large number of tRNA rearrangements in comparison to P. inermis and other monogeneans. Phylogenetic analyses conducted using Bayesian inference and maximum likelihood methods, complemented with partitioning, consistently support the sister-group relationship of T. monenteron (Tetraonchidae) and P. inermis (Tetraonchoididae). This is also partially supported by the 28S rDNA data and two morphologic apomorphies. This close relationship of Tetraonchidae and Tetraonchoididae challenges the latest major morphology-based classification, which proposed obsoletion of the Tetraonchidea order, and grouped Tetraonchoididae into the Gyrodactylidea Glade. The validity of this order shall have to be further confirmed with more data. (C) 2019 Elsevier B.V. All rights reserved.</p
Spatial pattern and determinants of global invasion risk of an invasive species, sharpbelly Hemiculter leucisculus (Basilesky, 1855)
No full textInvasive species have imposed huge negative impacts on worldwide aquatic ecosystems and are generally difficult or impossible to be eradicated once established. Consequently, it becomes particularly important to ascertain their invasion risk and its determinants since such information can help us formulate more effective preventive or management actions and direct these measures to those areas where they are truly needed so as to ease regulatory burdens. Here, we examined the global invasion risk and its determinants of sharpbelly (Hemiculter leucisculus), one freshwater fish which has a high invasive potential, by using species distribution models (SDMs) and a layer overlay method. Specifically, first an ensemble species distribution model and its basal models (developed from seven machine learning algorithms) were explored to forecast the global habitat-suitability and variables importance for this species, and then a global invasion risk map was created by combining habitat-suitability with a proxy for introduction likelihood (entailing propagule pressure and dispersal constraints) of exotic sharpbelly. The results revealed that (1) the ensemble model had the highest predictive power in forecasting sharpbelly's global habitat-suitability; (2) areas with high invasion risk by sharpbelly patchily spread over the world except Antarctica; and (3) the Human Influence Index (HII), rather than any of the bioclimatic variables, was the most important factor influencing sharpbelly' future invasion. Based on these results, the present study also attempted to propose a series of prevention and management strategies to eliminate or alleviate the adverse effects caused by this species' further expansion. (C) 2019 Elsevier B.V. All rights reserved.</p