Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
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Raman-Activated Droplet Sorting (RADS) for Label-Free High-Throughput Screening of Microalgal Single-Cells
No full textRaman-activated cell sorting (RACS) has attracted increasing interest, yet throughput remains one major factor limiting its broader application. Here we present an integrated Raman-activated droplet sorting (RADS) microfluidic system for functional screening of live cells in a label-free and high-throughput manner, by employing AXT-synthetic industrial microalga Haematococcus pluvialis (H. pluvialis) as a model. Raman microspectroscopy analysis of individual cells is carried out prior to their microdroplet encapsulation, which is then directly coupled to DEP-based droplet sorting. To validate the system, H. pluvialis cells containing different levels of AXT were mixed and underwent RADS. Those AXT-hyperproducing cells were sorted with an accuracy of 98.3%, an enrichment ratio of eight folds, and a throughput of similar to 260 cells/min. Of the RADS-sorted cells, 92.7% remained alive and able to proliferate, which is equivalent to the unsorted cells. Thus, the RADS achieves a much higher throughput than existing RACS systems, preserves the vitality of cells, and facilitates seamless coupling with downstream manipulations such as single-cell sequencing and cultivation
Iridium Clusters Encapsulated in Carbon Nanospheres as Nanocatalysts for Methylation of (Bio)Alcohols
No full textC-H methylation is an attractive chemical transformation
for C-C bonds construction in organic chemistry, yet eff icient
methylation of readily available (bio-)alcohols in water using
methanol as sustainable C1 feedstock is limited. Herein, yolk-shellstructured
mesoporous carbon nanospheres encapsulated Ir
nanocatalysts (Ir@YSMCNs) have been synthesized for this
transformation. Monodispersed Ir clusters (ca. 1.0 nm) w ere in situ
encapsulated and spatially isolated within YSMCNs by a silicaassisted
sol-gel emulsion strategy. A selection of (bio-)alcohols (19
examples) was selectively methylated in aqueous phase with goodto-
high yields over the developed Ir@YSMCNs. The improved
catalytic eff iciencies in terms of activity, selectivity together w ith the
good stability and recyclability were contributable to the ultrasmall Ir
clusters with oxidation chemical state as a consequence of the
conf inement ef fect of YSMCNs w ith interconnected nanostructures
Hydrogenolysis of lignin model compounds into aromatics withbimetallic Ru-Ni supported onto nitrogen-doped activated carboncatalyst
No full textPullulan production from synthetic medium by a new mutant of Aureobasidium pullulans.Prep Biochem Biotechnol
No full textProperties of nanocelluloses and their application as rheology modifier in paper coating.
No full textIn this study, different nanocellulose (NC) products were manufactured from corncob residue (CCR) through
sulfuric acid hydrolysis, formic acid hydrolysis, and TEMPO-mediated oxidation methods (the products were referred as SCN,
FCN, and TCN, respectively). The properties of NC products and their impact on rheological behavior of paper coatings were
comparatively studied. Results showed that compared to SCN and TCN, FCN exhibited large dimensions, limited negative
surface charge, and poor stability in their aqueous suspensions, while the FCN aqueous suspension displayed the highest
viscoelastic modulus due to the formation of highly entangled network. In paper coatings, SCN exhibited superior thickening and
promoted rheological function due to their highly charged surface and strong interactions with pigments and immobilized water
molecules, in comparison with other NC products. This study verified that the NC derived from CCR could be utilized as green
and renewable additives to improve rheological properties for paper coatings
Recent Progress on the Utilization of Chitin/Chitosan for Chemicals and Materials. In Book: Fuels, Chemicals and Materials from the Oceans and Aquatic Sources. Eds.: Ning Yan, Francesca Kerton. Wiley, June 23rd, 2017.
No full textHigh titer mevalonate fermentation and its feeding as building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coli.
No full textIsoprenoids are important fine chemicals as material monomers, advanced fuels and pharmaceuticals. A variety of natural isoprenoids can be synthesized by engineered microbial strains. This work established a process by dividing the current isoprenoid pathway into the upstream fermentation process, from sugar to mevalonate (MVA), and the downstream process, from MVA to the target isoprenoids. The results showed that significant differences existed in the process conditions between the upstream and downstream fermentations. After individually optimizing the process conditions, the upstream MVA production (84.0 g/L, 34.0% and 1.8 g/ L/h) and downstream isoprene production (11.0 g/L and 0.23 g/L/h) were greatly improved in this two-step process. Flask fermentation experiments also confirmed that two-step route can significantly improve the sabinene titer to 150 mg/L (6.5-fold of the sabinene titer in an earlier flask study of our lab). Therefore, the two-step route proposed in this study may have potential benefits towards the current isoprenoids production directly from glucose. The high titer and yield of MVA indicate that MVA has great potential to be more broadly utilized as starting precursor in synthetic biology
Draft Genome Sequences of Nine Cyanobacterial Strains from Diverse Habitats
No full textHere, we report the annotated draft genome sequences of nine different
cyanobacteria, which were originally collected from different habitats, including hot
springs, terrestrial, freshwater, and marine environments, and cover four of the five
morphological subsections of cyanobacteria
Efficient Route toward N-‑Unsubstituted Thienoisoindigo Suitable for Side-Chain Engineering
No full textA highly efficient and easily scalable synthetic method toward N-unsubstituted thienoisoindigo (TII) is developed with more than 25% overall yield, which is higher than the reported cases, and allows facile side-chain manipulation of the thienoisoindigo core