Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
Not a member yet
3266 research outputs found
Sort by
高效分离去除酚类化合物的新型吸附树脂的制备、表征、性能与机制.
No full text本研究优化了氯甲基化的苯乙烯-二乙烯苯树脂的后交联反应条件，并对不同条件下得
到的树脂的反应程度、比表面结构及孔道、官能团进行了表征。应用得到的HF-06 树脂，吸附
只含有酚羟基的高水溶性酚类物质苯酚、对苯二酚和间苯三酚。通过考察HF-06 在不同pH 值、
温度条件下的吸附性能，发现该树脂吸附容量存在苯酚>对苯二酚>间苯三酚的规律，吸附容量
是XAD-4 树脂的2.20 倍，比NDA150 等商业树脂高15%以上。吸附过程以物理作用为主并兼
有部分化学作用，树脂与吸附质分子之间同时存在着范德华力和氢键作用
A novel autolysis system controlled by magnesium and its application to poly (3-hydroxypropionate) production in engineered Escherichia coli.
No full textThe release of intracellular products, especially polyhydroxyalkanoates, is still a great challenge in
industry. To solve this bottleneck, a novel autolysis system strictly controlled with magnesium was
constructed and applied to poly(3-hydroxypropionate) production in engineered Escherichia coli.
The autolysis system was constructed by inserting the 50untranslated region (50UTR) behind
promoter PmgtA with lysis genes (S, R, and Rz, from E. coli) overexpressed. The autolysis system
functioned well (lysis efficiency of more than 90%) in the P3HP producer with double plasmids
containing lysis genes and P3HP biosynthesis genes, whereas the P3HP production was reduced
due to plasmid losses. After the autolysis genes and P3HP biosynthesis genes were integrated into
one plasmid, the P3HP content of 72.7% (2.4 times of the control) and the plasmid stability of 79.8
§ 3.1% were achieved in strain Q2646 with promoter PmgtA-UTR. However, the strain Q2647 with
promoter PmgtA could not accumulate P3HP because of rapid cell lysis. The novel autolysis system
activated in Mg2C-depleted conditions proves to be feasible for polyhydroxyalkanoates production,
which may have great application potential for other intracellular products
A versatile platform to achieve mechanically robust mussel-inspired antifouling coatings via grafting-to approach
No full textAlthough significant progress has been made in mussel-inspired antifouling coatings, most of them suffer from low mechanical stability. Herein, we present a facile and efficient method to fabricate mechanically robust mussel-inspired antifouling coatings. A polyvinyl alcohol (PVA)-based mussel-inspired coating material, which exhibits the highest adhesion capability (always at 5B level in a tape adhesion test based on the ASTM D3359 method) and excellent anti-abrasive properties (little mass loss after 1000 abrasion cycles), is used as a universal platform for further modification to introduce antifouling properties. Intriguingly, the hydroxyl groups in this PVA-based coating material are used as the anchor for the installation of either hydrophilic or hydrophobic segments (or both) via a grafting-to approach. Single modifiers, methoxypolyethylene glycol (MPEG), sodium 2-hydroxyethanesulfonate (SHS) and 1H,1H,2H,2H-perfluorooctan-1-ol (PFO), and complex modifiers, MPEG/PFO, are tethered onto the coating through an effective urethane bond formation reaction to endow the surfaces with antifouling efficacy. The functionalized surfaces are shown to be effective in preventing bovine serum albumin (BSA) adsorption and in reducing bacterial (Gram-positive bacteria S. aureus and Gram-negative bacteria (E. coli) adhesion. More importantly, such modification does not influence the strong adhesion and excellent antiabrasion properties of the coating. To the best of our knowledge, this is the first example of merging excellent mechanical properties and antifouling capability for mussel-inspired coatings, which might find niches in a broad range of applications in the industrial and biomedical fields
A Strategy to Make High Voltage LiCoO2 Compatible with Polyethylene Oxide Electrolyte in All-Solid-State Lithium Ion Batteries
No full text
A Rational Design of High-Performance Sandwich-Structured Quasisolid State Li-O-2 Battery with Redox Mediator
No full textThe increasing interest in Li-O-2 battery arises from its unparalleled theoretical energy density. Nevertheless, the poor reversibility of cathode reaction and unstable characteristic of Li anode hinder its further application. To address these issues, a high-performance sandwich-structured quasisolid polymer electrolyte (QSPE) is designed to meet the requirement of both cathode and anode. For the first time, lithiated Nafion ionomer (Li-Nafion) is introduced into Li-O-2 cell to separate "catholyte" and "anolyte." Redox mediator (RM) is introduced into gel-like catholyte, based on polymethymethacrylate, to achieve high capacity and reversibility. Polypropylene carbonate is chosen as solid-state anolyte for enhancing interface stability of lithium anode. It is demonstrated that the QSPE exhibits excellent permselectivity to block RM shuttling, as well as good ionic conductivity and high electrochemical window. A solution mechanism formation of discharge product is demonstrated in the Li-O-2 cell with QSPE and the RM works well for cycles at room temperature. This sandwich-structured design strategy will provide a new pathway to promote the properties of Li-O-2 battery
Pollution levels of DDTs and their spatiotemporal trend from sediment records in the Southern Yellow Sea, China
No full text
Effect of Glycerol Pretreatment on Levoglucosan Production from Corncobs by Fast Pyrolysis
No full textIn this manuscript, glycerol was used in corncobs' pretreatment to promote levoglucosan production by fast pyrolysis first and then was further utilized as raw material for chemicals production by microbial fermentation. The effects of glycerol pretreatment temperatures (220-240 degrees C), time (0.5-3 h) and solid-to-liquid ratios (5-20%) were investigated. Due to the accumulation of crystalline cellulose and the removal of minerals, the levoglucosan yield was as high as 35.8% from corncobs pretreated by glycerol at 240 for 3 h with a 5% solid-to-liquid ratio, which was obviously higher than that of the control (2.2%). After glycerol pretreatment, the fermentability of the recovered glycerol remaining in the liquid stream from glycerol pretreatment was evaluated by Klebsiella pneumoniae. The results showed that the recovered glycerol had no inhibitory effect on the growth and metabolism of the microbe, which was a promising substrate for fermentation. The value-added applications of glycerol could reduce the cost of biomass pretreatment. Correspondingly, this manuscript offers a green, sustainable, efficient and economic strategy for an integrated biorefinery process
