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Identification of over expressed proteins in oral submucous fibrosis by proteomic analysis
No full textEarly detection and identification of oral pre-malignancy or malignancy help in management of the disease and improve survival rates. Oral submucous fibrosis (OSMF)
is amajor threat to public health worldwide and especially in SoutheastAsian countries.Identification of biomarkers is a necessary step toward early diagnosis and treatment. In
this study, differentially expressed proteins between oral submucous fibrotic tissue and normal control tissues were recorded by proteomic analysis using two dimensional
electrophoresis (2DE) andMALDI TOF mass spectrometry. By proteomic analysis, 15 proteins were found to be upregulated and 10 proteins downregulated in the OSMF
tissues than the control tissues; among these identified proteins, Hsp-70 1B,Calreticulin, and Lumican variant exhibited higher expression in OSMF tissues compared to the
control tissues. Immunohistochemical analysis also showed elevated expression of these in OSMF tissues. Further validation was done by real time quantitative RT-PCR
analysis; gene expression of Hsp-70 1B, Calreticulin, and Lumican variant were significantly increased (6.2-, 3.3-, 2.8- fold, respectively), whereas Enolase 1 was
decreased by 0.5 fold in the OSMF tissues, consistent with proteomic results. The expression of proteins indicates that various cellular signaling pathways must be
involved in the processes of fibrosis and suggests that expressed protein molecules play an important role in the pathogenesis of OSMF. These identified proteins may be
potentially used in future studies of OSMF enabling to determine diagnostic marker or therapeutic targets of this precancerous condition of oral cavit
SYNTHESIS, PHYSICOCHEMICAL AND BIOLOGICAL EVALUATION OF NOVEL SITE SPECIFIC SPECT-RADIOPHARMACEUTICALS BASED ON 99mTc(CO)3(H2O)3-SYNTHON CORE
No full textSTUDY OF THE INTERACTIONS OF NOVEL QUINOXALINE BASED SMALL MOLECULES WITH NUCLEIC ACIDS AND THEIR POTENTIAL APPLICATIONS
No full textCobalt-Catalyzed Directed sp2CH Acetoxylation of ArenesEmploying Mn(OAc)3·2H2O as Acetoxy Source
No full textMultiple target specific chemical combinations for development of potential anti-cancer therapy
No full text
DESIGN, SYNTHESIS AND BIOLOGICAL EVALUATION OF SMALL MOLECULE IMMUNOMODULATORS TARGETING ENDOSOMAL TLRS AND PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL
No full textDesign and Synthesis of Novel Quinoxaline Small Molecules and Evaluation of their Biophysical and Nucleic acid Binding Properties
Full text linkINNATE IMMUNE REGULATION OF METABOLIC SYNDROME
No full textEvolution of multicellular organisms from unicellular ones was focussed mainly around the availability of nutrients. Ability of mammals to cope with this nutrient limitation through control of its usage and storage internally gives us survival advantage over other organisms (1). But during this evolution the focus was put mainly on conserving nutrients within the body when there was scarcity in the surroundings, but now our metabolic system is challenged with a unique and unprecedented problem which is nutrient excess. Its seems our evolution was so focussed on the conservation of the nutrients; the present situation of the nutrient excess has become a baffling problem for our system. This scenario is especially evident in developing and developed world, where obesity and associated metabolic disorders have become an epidemic. Normally metabolism and immune system were thought to be different entities, but now their role in shaping up each other is being well appreciated (2). It has been proposed that the immune system can sense and if required can impact the metabolic state of the body and depending on which it can either assume inflammatory or regulatory role in the metabolically active tissues (3)
With ever increasing burden of metabolic syndrome on medical expenses around the world reaching astronomic levels and concomitant rise in the incidence of obesity, the aforementioned association of immune system and obesity associated metabolic syndrome is under spotlight with a hope to manage this epidemic through tweaking their interplay. The role of immune system in our body extends beyond protection from invading pathogens as it plays a major role in shaping out systemic metabolism.
Apart from its role in autoimmune disease like type 1 diabetes where immune cells destroy pancreatic beta cells and disrupt glucose homeostasis, obesity associated inflammation also plays a significant role in the development of insulin resistance, which is inability to respond to circulating insulin that in turn disrupts glucose homeostasis and is central to the etiology of type 2 diabetes (1,2)