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Anatomy, structure and function: Understanding extremes in fear and anxiety by in vivo imaging techniques
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Reliability and comparison of 3-dimensional surface imaging of the face using a hand-held and whole body surface scanner
Get PDFObjective: 3-Dimensional surface (3DSI) imaging has been shown to be a useful tool for plastic surgeons in the preoperative, intraoperative and postoperative setting. And to the knowledge of the authors no data about the reproducibility and accuracy of 3-Dimensional surface imaging of the face using a whole-body scanner is available. Thus, the objective of this investigation was to assess the reproducibility of facial scans acquired using a whole-body imaging device and to compare the precision of distance measurements in the face using a hand-held surface imaging device and a whole-body surface imaging device. Furthermore, the reproducibility of the whole body scanner was investigated.
Material and Methods: This investigation enrolled a total of 22 healthy volunteers with a mean age of 29.36 years. Two consecutive 3-D images of the volunteers were obtained utilizing a whole-body imaging device(WB360) and a hand-hold imaging device(Vectra H2). For the whole-body imaging predefined distances in the face were performed in each scan and compared. Furthermore, surface deviation between two consecutively captured scans was assessed.
Results: For the reliability of whole-body scan, the distance with the smallest statistical significance was found to be at the nose with p = 0.998, while the biggest statistical significance was found in the midface with p = 0.658. The area with the biggest surface deviation between the superimposed scans was the neck with a RMS of 1.62 ± 1.71 mm and the area with the smallest surface deviation was the forehead with a RMS of 0.17 ± 0.05 mm. For the comparison of the both scanners our results revealed that the measured difference between the length and the standard reference did not differ statistically significant between the two investigated devices in all investigated areas of the face (p > 0.266), however the measured difference of the width and the width of the standard reference differed statistically significant in all areas of the face across the investigated devices (p < 0.032).
Conclusion: The whole – body-imaging device investigated in this study can be utilized to capture the face and provides enough accuracy to compare scans. Even though not directly investigated, it can be hypothesized that the error caused by repositioning the patient between a baseline and a follow – up scan will not be too big to consider measurements performed with the whole – body-imaging device as impractical. Both, measurements obtained from scans acquired using the hand held imaging device and the whole – body-imaging device differed significantly from the standard reference. Users should be aware of deviations when obtaining 3DSI using the presented imaging devices but should not refrain from using them, as the absolute differences might be too small to play a role in both, clinical and research, setting
Retrospektive Auswertung eines neuen Therapie-Schemas bei allergisch-bronchopulmonaler Aspergillose für Patienten mit Zystischer Fibrose
Get PDFThe expression of Sirtuin1 and its role in ovarian cancer
Get PDFSIRT1 has been a popular subject in the scientific research field since its detection in 1990. The function of this protein varies greatly depending on the tissue. Recent studies focused on SIRT1, especially in tumors. SIRT1 has been revealed to modulate cancer cell growth, metastasis, and invasion in numerous tumors. However, SIRT1 has been barely studied in gynecological tumors.
The purpose of our study was to evaluate the effect of SIRT1 on ovarian cancer. First, we analyzed the relationship between the intensity of SIRT1 and RXR-alpha staining and clinical prognosis in ovarian cancer by immunohistochemistry. To further study the therapeutic effects of SIRT1 activator Resveratrol, we used MTT, M30, BrdU, and other methods to test the function of resveratrol on ovarian cancer cell lines A2780, UWB1, 289, and A2780cis, including proliferation and apoptosis. Finally, the relationship between RXR-alpha and SIRT1 proteins in ovarian cancer treated with Resveratrol was studied by western blot.
Studies have shown that nuclear RXR-alpha and SIRT1-expression are significantly connected with a better outcome and overall survival rates in advanced ovarian cancer. Resveratrol had a direct effect on ovarian cancer: it suppressed the growth and proliferation of ovarian cancer cells and even increased their apoptosis. At the protein level, resveratrol (100 μM, 24h) up-regulated the expression of RXR-alpha in the anti-carboplatin cell line A2780cis and down-regulated the expression of SIRT1 in A2780. In conclusion, SIRT1 may have a suppressive role in ovarian cancer, especially in advanced ovarian cancer
Quantitativ-stereologische Analyse der Kiemenmorphologie von in der ökotoxikologischen Forschung verwendeten Regenbogenforellen (Oncorhynchus mykiss)
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Get PDFDie Dissertation untersucht die herzoglich-bayerische Finanzverwaltung im Zeitraum von 1550 bis 1618 auf drei Verwaltungsebenen: der Oberbehörde (Hofkammer), der Mittelbehörde (am Beispiel des Rentamts Landshut) und der Unterbehörde (am Beispiel des Pflegamts Moosburg). Im Mittelpunkt stehen die organisatorischen Strukturen, die schriftliche Kommunikation und die räumliche Mobilität.
Die Finanzverwaltung des Herzogtums Bayern erfährt mit der Gründung der Hofkammer 1550 den entscheidenden Impuls zur Ausbidlung einer modernen hierarchischen Vewaltungsstruktur Die operative Schriftlichkeit, auf der die zentralisierte Finanzverwaltung basiert, ging mit einer systematisch gestalteten, routinierten Mobilität der Amtsträger im Herzogtum einher.
Isabella Hödl-Notter absolvierte ihr Bachelor- und Masterstudium der Bayerischen Landesgeschichte und Geschichte der Frühen Neuzeit an der LMU München und verbrachte ein ERASMUS-Semester (WS 2013/14) an der École nationale des chartes in Paris. 2022 erfolgte die Promotion im Fachbereich Neuere und Neueste Geschichte an der LMU München.The dissertation examines the ducal Bavarian financial administration in the period from 1550 to 1618 at three administrative levels: the higher authority (Hofkammer), the middle authority (using the example of the Rentamt Landshut), and the lower authority (using the example of the Pflegamt Moosburg). The focus is on organizational structures, written communication, and spatial mobility.
With the establishment of the Hofkammer in 1550, the financial administration of the Duchy of Bavaria received the decisive impetus for the development of a modern hierarchical administrative structure. The operational written communication on which the centralized financial administration was based went hand in hand with the systematically organized, routine mobility of officials in the duchy.
Isabella Hödl-Notter completed her bachelor's and master's degrees in Bavarian history and early modern history at LMU Munich and spent an ERASMUS semester (2013/14) at the École nationale des chartes in Paris. In 2022, she received her doctorate in modern and contemporary history from LMU Munich
Characterization of peripheral immune events in primary colorectal cancer patients
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The interaction between tumor and immune cells is known to affect the progression of colorectal cancer (CRC), but the effect of CRC cells on the systemic immunity remained unclear. We aimed to perform a comprehensive evaluation of circulating immune subsets and gene expression analysis of CRC patients.
Methods
The subset composition and phenotype of B and T lymphocytes, neutrophils, monocytes, dendritic cells (DCs), myeloid-derived suppressor cells (MDSCs), NK, and NKT cells were investigated in peripheral blood samples from 12 CRC patients and 11 healthy controls by multicolor flow cytometry. Circulating immune subsets were utilized to construct a diagnostic model. Furthermore, we performed the bioinformatics analyses to obtain the differential expression genes (DEGs) and immune cells between CRC patients and normal controls. Correlation analysis was conducted to discover regulatory genes and clinical test parameters associated with distribution of circulating immune subsets.
Results
In contrast to healthy controls, CRC patients had a reduced proportion of B and T lymphocytes, T helper (Th) cells, non-classical monocytes, DCs, and an increased proportion of polymorphonuclear MDSCs, as well as reduced expression of CD69 on both CD56dim and CD56bright NK cells. A diagnostic model integrating seven immune subsets was constructed to discriminate CRC patients and healthy controls with an AUC of 1.000 (95% CI 1.000-1.000). Moreover, NR3C2, CAMK4 and TRAT1 were identified as candidate genes regulating the number of circulating Th cells in CRC patients. Right-sided CRC patients had a similar systemic immune landscape with left-sided ones. In addition, we found a robust positive correlation between immune subsets and three clinical test parameters, including gamma-glutamyltransferase, aspartate aminotransferase, and alanine aminotransferase.
Conclusions
The immune suppression of systemic immune responses is evident in CRC patients. The altered composition of circulating immune subsets in CRC could complement the regional immune status of the tumor microenvironment and contribute to the discovery of immune-related biomarkers for the diagnosis of CRC.Das Zusammenspiel von Tumor- und Immunzellen kann das Fortschreiten von Darmkrebs (CRC) beeinflussen. Bislang ist die Auswirkung von kolorektalen Karzinomzellen auf die systemischen Immunprofile unzureichend untersucht. Das Ziel dieser Arbeit war die detailierte Charakterisierung von Immunzellen im peripheren Blut von Patienten mit kolorektalem Karzinom.
Die Zusammensetzung der Untergruppen und der Immunphänotyp von B- und T-Lymphozyten, Neutrophilen, Monozyten, dendritischen Zellen (DCs), myeloiden Suppressorzellen (MDSCs), NK- und NKT-Zellen wurden in peripheren Blutproben von 12 CRC-Patienten und 11 gesunden Kontrollpersonen mittels Multicolor-Durchflusszytometrie untersucht. Logistische Regressionsanalysen und maschinelle Lernalgorithmen (Support Vector Machine Learning) wurden durchgeführt, um ein diagnostisches Modell zur Unterscheidung von CRC-Patienten und gesunden Kontrollpersonen anhand der Immunzelluntergruppen zu erstellen. Darüber hinaus wurden die Expressionmuster der peripheren Blut- und Gewebeproben verwendet, um die Gene mit unterschiedlicher Expression (DEGs) bei CRC-Patienten im Vergleich zu normalen Kontrollen zu analysieren. Es wurde eine Korrelationsanalyse durchgeführt, um DEGs zu finden, die mit der Verteilung von Untergruppen von Immunzellen assoziiert sind und um die zugrunde liegende Beziehung zwischen Immunzellzusammensetzungen und klinischen Testparametern bei CRC-Patienten weiter zu untersuchen.
Ergebnisse: Im Gegensatz zu gesunden Kontrollpersonen wiesen CRC-Patienten einen geringeren Anteil an B- und T-Lymphozyten, T-Helferzellen (Th-Zellen), nicht-klassischen Monozyten, DCs und einen erhöhten Anteil an polymorphkernigen MDSCs sowie eine geringere Expression von CD69 sowohl auf CD56dim- als auch auf CD56bright-NK-Zellen auf. Ein diagnostisches Modell, das sieben Immununtergruppen integriert, wurde erstellt, um CRC-Patienten und gesunde Kontrollen mit einer AUC von 1.000 (95% CI 1.000-1.000) zu unterscheiden. Darüber hinaus wurden NR3C2, CAMK4 und TRAT1 als Kandidatengene identifiziert, die di