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Chemo-mechanical properties of the new bioceramic cement PBS CIMMO® compared to MTA REPAIR HP Angelus® cement
No full textKellen C. S. Casque - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento.A grafia do último sobrenome de uma das autoras está incorreto. O correto é Gasque e não Casque.Objective: To evaluate the bond strength (BS), pH and solubility (Sol) of a new PBS HP®
bioceramic cement compared to MTA REPAIR HP® cement.
Material and methods: Twenty human premolars were selected for BS analysis. Roots
were cut into thirds (cervical, medium and apical) and filled with one of the cements (n=10):
PC (PBS HP) or MC (MTA REPAIR HP). Half of these samples were subjected to 2,000
thermal cycles. After 48 hours, the BS test was performed in a universal testing machine
(0.5mm/min). Cylindrical specimens (1.0mm in diameter and 10mm in height) were made
with PC or MC and immersed in distilled water for pH analysis. After 0.3 and 24 hours, the
pH was measured. Solubility was evaluated through the mass loss of the samples after 24
hours. Teflon rings of 20mm in diameter and 1.5mm in height were filled with PC or MC
and kept at 37ºC in 100% humidity. Descriptive and exploratory analyzes of BS, pH and
Sol data were performed (p<0.05).
Results: There was no significant difference in BS between the two types of cement.
Thermo cycling did not affect the BS (p>0.05). MC cement showed significantly higher pH
values than the PC at the initial and 3 hours (p<0.05). There was no difference between them
(p>0.05) after 24 hours. There was no significant difference regarding solubility (p>0.05).
Conclusion: Bioceramic cements have similar properties, as they did not differ in relation
to dentin bond strength, pH after 24 hours and solubility
Detrimental Effect of Ozone on Pathogenic Bacteria
No full textBackground: Disinfection of medical devices designed for clinical use associated or not
with the growing area of tissue engineering is an urgent need. However, traditional disinfection
methods are not always suitable for some biomaterials, especially those sensitive to chemical, thermal,
or radiation. Therefore, the objective of this study was to evaluate the minimal concentration of
ozone gas (O3) necessary to control and kill a set of sensitive or multi-resistant Gram-positive
and Gram-negative bacteria. The cell viability, membrane permeability, and the levels of reactive
intracellular oxygen (ROS) species were also investigated; (2) Material and Methods: Four standard
strains and a clinical MDR strain were exposed to low doses of ozone at different concentrations and
times. Bacterial inactivation (cultivability, membrane damage) was investigated using colony counts,
resazurin as a metabolic indicator, and propidium iodide (PI). A fluorescent probe (H2DCFDA) was
used for the ROS analyses; (3) Results: No reduction in the count colony was detected after O3
exposure compared to the control group. However, the cell viability of E. coli (30%), P. aeruginosa
(25%), and A. baumannii (15%) was reduced considerably. The bacterial membrane of all strains was
not affected by O3 but presented a significant increase of ROS in E. coli (90 14%), P. aeruginosa
(62.5 19%), and A. baumanni (52.6 5%); (4) Conclusion: Low doses of ozone were able to interfere
in the cell viability of most strains studied, and although it does not cause damage to the bacterial
membrane, increased levels of reactive ROS are responsible for causing a detrimental effect in the
lipids, proteins, and DNA metabolism
Evaluación del desempeño masticatorio y nivel de satisfacción de usuarios de prótesis parciales removibles: énfasis en la importancia de las PPRs en la era de la implantología
No full textObjetivo: Avaliar a performance mastigatória e o nível e satisfação dos indivíduos parcialmente desdentados, após serem reabilitados com próteses parciais removíveis (PPRs), com ênfase na importância desse tratamento para com a saúde bucal no contexto da odontologia atual onde os implantes dentários osseointegrados tem sido bastanteutilizado.Metodologia: 23 pacientes parcialmente desdentados foram reabilitados com PPRs na clínica de prótese dentária da Unifal-MG. Foram executados, antes e depois do tratamento reabilitador, questionários avaliando o nível de satisfação desses pacientes no que diz respeito a estética e função mastigatória, e testes de performance mastigatória (PM) utilizando um material simulador de alimento a partir do silicone de condensaçãoZetaplus –Zermack. Resultados: Após o tratamento reabilitador, os pacientes relataram sentirem-se mais satisfeitos em relação a estética e função dos dentes. Na análise estatística, O valor-pdo teste tencontrado foi de 0,002, levando à rejeição da hipótese nula e conclusão de que o diâmetro geométrico médio (DGM) das partículas mastigadas é menor após o tratamento reabilitador com prótese parcial removível. Conclusão: O tratamento com PPR proporciona melhora na PM e no nível de satisfação dos pacientes no que diz respeito a estética e função mastigatória sendo, portanto, de extrema importância para a saúde pública, uma vez que impacta positivamente na melhora da qualidade de vida dos indivíduos.Objective: To evaluate the masticatory performance and the level and satisfaction of partially edentulous individuals, after being rehabilitated with removable partial dentures (RPDs), emphasizing the importanceof this treatment for oral health in the context of current dentistry where osseointegrated dental implants have been widely used.Methods: 23 partially edentulous patients were rehabilitated with RPDs at the dental prosthesis clinic at Federal university of Alfenas. Questionnaires were carried out before and after the rehabilitation treatment, evaluating the level of satisfaction of these patients regarding to the aesthetics and function of chewing food. Masticatory performance (MP) tests were made using a food simulator from condensation silicone Zetaplus –Zermack.Results: After the rehabilitation treatment, the patients reported feelingmore satisfied in relation to the aesthetics and function of their teeth. In the statistical analysis, the p-value of the t test found was 0.002, leading to the rejection of the null hypothesis and the conclusion that the geometric mean diameter (GMD) of chewed particles is smaller after the rehabilitation treatment with removable partial dentures.Conclusion: Treatment with RPD provides an improvement in MP and in the level of patient satisfaction with regard to aesthetics and masticatory function, and, it is therefore extremely important for public health as it has a positive impact on improving the quality of life of individuals.Objetivo: Evaluar el desempeño masticatorio y el nivel y satisfacción de individuos parcialmente desdentados, luego de ser rehabilitados con prótesis parciales removibles (PPRs), enfatizando la importancia de este tratamiento para la salud oral en el contexto de la odontología actual donde los implantes dentales osteointegrados han sido ampliamente utilizados.Metodología: 23 pacientes parcialmente edéntulos fueron rehabilitados con PPRs en la clínica de prótesis dental de Unifal-MG. Se realizaron cuestionarios antes y después del tratamiento de rehabilitación, evaluando el nivel de satisfacción de estos pacientes con respecto a la estética y la función masticatoria, y pruebas de rendimiento masticatorio (RM) utilizando un material simulador de alimentos de Zetaplus -Zermack silicona de condensación. Resultados: Después del tratamiento de rehabilitación, los pacientes reportaron sentirse más satisfechos con la estética y función de los dientes. En el análisis estadístico, el valor p de la prueba t encontradofue de 0,002, lo que lleva al rechazo de la hipótesis nula y a la conclusión de que el diámetro geométrico medio (DGM) de las partículas masticadas es menor después del tratamiento de rehabilitación con prótesis parcial removible.Conclusión: El tratamiento con PPRs proporciona una mejora en el RM y en el nivel de satisfacción del paciente en cuanto a la estética y la función masticatoria, por lo que es de suma importancia para la salud pública, ya que impacta positivamente en la mejora de la calidad de vida de los pacientes
Development of a new theragnostic based on DNA aptamers against heparinase1 for the treatment of breast cancer
No full textOs autores externos submeteram sua publicação para apresentação de trabalho no evento “International Symposium on Immunobiologicals”, que foi coordenado e organizado pelo Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), da Fundação Oswaldo Cruz
Subacute infective endocarditis caused by Bacillus cereus in a patient with Systemic Lupus Erythematosus
No full textA rare and difficult to diagnose case of subacute infective endocarditis caused by Bacillus cereus in a patient with systemic lupus erythematosus and Libman-Sacks endocarditis has been reported. Our aim is to highlight the importance of molecular methods such as MALDI-TOF and PCR to explain clinical and epidemiological issues about infections caused by unusual pathoge
Nasal COVID-19 vaccine
No full textOs autores externos submeteram sua publicação para apresentação de trabalho no evento “International Symposium on Immunobiologicals”, que foi coordenado e organizado pelo Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), da Fundação Oswaldo Cruz
Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil
No full textProdução científica do Laboratório de Inovações em Terapias, Ensino e Bioprodutos.Produção científica do Laboratório de Carrapatos e Outros Artrópodes Ápteros.This study was conducted with the support of Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES—funding code 001) and Plano de Objetivos e Metas (POM) of the Laboratório de Inovações em Terapias, Ensino e Bioprodutos – LITEB and Laboratório de Referência Nacional em Vetores das Riquetsioses (LIRN), Instituto Oswaldo Cruz, Fiocruz.Dirofilaria immitis is the causative agent of canine heartworm disease, a severe health problem in dogs, especially in coastal areas of tropical and subtropical regions of the world. We employed molecular methods to investigate the occurrence of canine infection by filarioids in five municipalities of Baixada Fluminense (Magé, Duque de Caxias, Guapimirim, Nova Iguaçu, and São João de Meriti), a non-endemic area of Rio de Janeiro State, Southeast Brazil. A total of 110 canine blood samples collected from 2017 to 2018 and positive for microfilariae at the modified Knott’s test were screened by cPCR targeting DNA fragments of the 12S rDNA gene for filarial nematodes. Seventy-seven samples (70%) tested positive at the molecular analysis. Of these, 72 were identified as D. immitis and 5 (4.5%) as Acanthocheilonema reconditum. Dirofilaria repens was not detected in the studied municipalities of Baixada Fluminense. This is the first record of D. immitis and A. reconditum in the Baixada Fluminense region, Rio de Janeiro State, Brazil. The prevalence of D. immitis cases in the five municipalities suggests the establishment and maintenance of its enzootic cycle in the studied region, which indicate vulnerability to the occurrence of epidemic cycles and, possibly, human cases.2030-12-3
Effects of Bauhinia forficata Link tea in the glycemic profile of diabetics' patients: a literature review
No full textA Revista Fitos é um periódico científico interdisciplinar, em acesso aberto, sem custos para autores e de difusão gratuita, com publicação em fluxo contínuo, organizado, gerenciado e editado pelo Centro de Inovação em Biodiversidade e Saúde (CIBS), do Instituto de Tecnologia em Fármacos (Farmanguinhos), da Fundação Oswaldo Cruz. Tendo a inovação em Biodiversidade e Saúde como escopo, o periódico publica artigos científicos inéditos e originais abordando a pesquisa, o desenvolvimento tecnológico e a inovação em Biodiversidade e Saúde e as inter-relações entre suas diversas dimensões. Os manuscritos deverão ser redigidos em português, inglês ou espanhol, do tipo: artigo de pesquisa, artigo de revisão, relato de experiência, comunicação breve, ensaio, perspectiva, resenha, carta e monografia de espécies da biodiversidade brasileira. Os autores deste artigo não possuem vínculo com a Fiocruz. Link da revista: https://periodicos.fiocruz.br/pt-br/revista-fitosNa cultura brasileira, o chá de Bauhinia forficata Link, conhecido popularmente por pata-de-vaca, é um forte exemplo de uso de plantas na medicina popular, sendo utilizado como tratamento alternativo de Diabetes mellitus devido aos seus efeitos que contribuem no controle glicêmico. Com objetivo de compreender os efeitos hipoglicemiantes do chá (infusão) de Bauhinia forficata Link em indivíduos com DM, realizou-se uma revisão bibliográfica, na qual foram selecionados 7 artigos de 31 documentos avaliados nos idiomas português e inglês, entre os anos 2010 e 2020. Estudos verificaram que a planta pata-de-vaca possui propriedades benéficas à saúde, como o efeito hipoglicemiante oriundo de compostos bioativos, como a kampferitrina e outros polifenóis, capazes de promover a inibição das enzimas salivares e pancreáticas αamilase e da borda em escova intestinal α-glicosidase, diminuição dos transportadores de glicose dependente de sódio (SGLT1), supressão da liberação de glicose pelo fígado e estímulo à captação de glicose pelos tecidos periféricos do organismo. Todavia, mais estudos em humanos ainda são necessários para consolidar o efeito da planta, visto que eles dependem da ingestão de chá de BFL com tratamento medicamentoso e, em alguns estudos, alguns indivíduos apresentaram resistência em aderir regularmente ao tratamento.In Brazilian culture, the consumption of Bauhinia forficata Link tea, popularly known as cow’s paw is an example of the plant use in the folk medicine, being used as an alternative treatment for diabetes due its effects of controlling glucose blood levels. Aiming at comprehending the hypoglycemic effects of Bauhinia forficata Link’s tea (infusion) in patients with DM, a literature review was performed in which 7. Studies verify that the cow's paw plant has beneficial properties to health, such as the hypoglycemic effect due to bioactive compounds, such as kaempferitrin and other polyphenols, capable of promoting inhibition of salivary and pancreatic enzymes α-amylase and gut border brush enzyme α-glycosidase, decrease of small intestine sodium-glucose linked transporter (SGLT1), suppression of glucose release by the liver and stimulation of glucose uptake by peripheral tissues of the body. However, studies in humans are insufficient to consolidate the benefits of the plant, as it depends on the adherence of the tea infusion consummation with medication treatment
The effects of dehydration and local soil on parasite recovery: A preliminary paleoparasitological evaluation on experimental coprolites
No full textExperimental paleoparasitological approaches have been used in order to optimize the methodology previously to the application in archeological
samples. In this study we evaluated the action of dehydration and local soil (Central Argentina) on the loss of parasite eggs in experimental coprolites,
using two parasitological techniques: spontaneous sedimentation and sucrose-flotation. Experimental coprolites comprised fresh human feces, positive
for Hymenolepis nana, Ascaris sp., and Enterobius vermicularis, submitted to controlled artificial dehydration. Experimental coprolites with soil addition
were prepared by mixing archeological sediment with equal mass of fresh feces. Helminth eggs were counted and eggs per gram were estimated in each
subsample. Statistical analyses were applied to compare subsamples before and after desiccation and with and without addition of soil sediment. The
performance of parasitological methods statistically differed, the sucrose flotation technique being the less effective when fresh feces and experimental
coprolites were analyzed. Partial deformation of eggs was observed via both techniques only in subsamples containing H. nana eggs. However, this was
not seen in Ascaris sp. subsamples, possibly due to eggshell composition. We found that sample desiccation significantly decreased the number of eggs
in the experimental coprolites. Mixing archeological sediment with the fecal material also resulted in significantly fewer eggs surviving, independent of
desiccation. This shows that climate and soil in which archeological fecal samples are found can strongly influence the survival of parasite eggs from past
populations. The small amount of parasite evidence often found in paleoparasitological analyses, including Central Argentina, could be attributed to the
action of taphonomic processes rather than to the real absence of infection in these ancient populations. Importantly, the study highlights the role of
local soil, confirmed for the first time by empirical data. The research provides valuable insights into the understanding of the paleoparasitological results
of the region and of general paleoparasitology
Public health indicators among female sex workers: biological an d behavioral surveillance survey in 12 Brazilian cities, 2016
No full textAs mulheres trabalhadoras do sexo (MTS) são consideradas uma população c have para o HIV. No B rasil, estima se que as MTS representem 1,2% da população feminina de 15 a 49 anos de idade, que corresponde a aproximadamente 690 mil de m ulheres. O presente trabalho tem o objetivo de divulgar indicadores de saúde pública entre MTS p esquisadas no segundo estudo de vigilância biológica e comportamental em 12 municípios brasileiros. O estudo transversal de vigilância biológica e comportamenta l foi realizado com 4328 MTS recrutadas por Respondent Driven Sampling (RDS) em 12 municípios br asileiros. O tamanho de amostra foi pré estabelecido pelo Departamento de Condições Crônicas e Infecções Sexualmente Transmissíveis (DCCI) do Ministério da Saúd e de 350 MTS em cada cidade. As participantes responderam a um questionário sociocomportamental e foram realizados te stes rápidos para HIV, sífilis, hepatites B e C. A análise estatística lev ou em consideração o desenho complexo de amostragem do recrutamen to por RDS Entre os resultados, destacam se: a prevalência da infecção pelo HIV vari ou de 0,2% em Campo Grande a 18, 2% em Salvador d iferenças significativas entre as cidades foram encontradas nas distribuições segundo escolaridade, renda, local de trabal ho, idade de início do trabalho sexual, e uso de drogas ilícitas ; a cobertura de exame de Papani colau variou de 53,4% 53,4%, em Recife, a 73%, em Porto Alegre e a de pré natal variou de 61,1%, em Salvador, a 99 ,0 %, em Curitiba ; um total de 67,0% de MTS relatou e star utilizando algum método contraceptivo, sendo esse percentual menor do que da população bras ileira em 13 pontos p ercentuais; ao contrário do perfil nacional em que o anticoncepcional oral é mais utilizado, o preservativo masculino foi o método mais utilizado pelas MTS (37,1%); aproximadamente um quinto das participantes já se sentiram discrimina das ou foram tratadas pior por ser TS e apenas 24,3% declararam ser MTS em um serviço de saúde. Em conclusão , os dados corroboram a situação de maior vulnerabil idade das MTS. Os resultados apresentados fornecem contribuições importantes no direcionamento d e intervenções locais . As limitações do estudo referem se ao método RDS utilizado no recrutamento das participantes, e inclui o efeito de homofilia, o pagamento de incentivos e o local de realização do estudo que pode afetar a composição das redes de acord o com as MTS que trab alham nas proximidades.Female Sex Workers (FSW) are recognized as an HIV key popu lation In Brazil, it is estimated that FSW represent 1.2% of the female population between 15 49 years old, which corresponds to approximately 690,000 women. Th is study aims at publicize public health indicators among FSW who participated in the 2 nd Biolog ical and Behavioral S urveillance Survey (BBSS) in 12 Brazilian cities. The cross sectional BBSS was performed with 4328 FSW recruited by Respondent Driven Sampl ing (RDS) in 12 Brazilian cities. The sample size of 350 FSW in each city was pre established by the Department o f Ch ronical Conditions and Sexually Transmitted Infections (DCCI Departamento de Condições Crônicas e Infecções Sexualmente Transmissíveis ) f rom the Ministry of Health. Participants answered a socio behavioral questionnaire and rapid tes ts for HIV, syphilis, hepatitis B and C were performed. The statistical analysis considered the complex sampling design of RDS recruitment. The following result s stand out: the prevalence of HIV infection ranged from 0.2% in Campo Grande to 18.2% in Salvad or; s ignificant diffe rences between cities were found in distributions according to educational level, income, workplace, age sex work started, and illicit drug use ; pap smear coverage ranged from 53,4%, in Recife to 73 ,0 in Porto Alegre and antenatal car e coverage ranged fro m 61.1% in Salvador to 99.0% in Curitiba; a total of 67.0% of FSW reported using some contraceptive method and this frequency is 13 percent age points lower than the Brazilian population; in opposition to the national profile in which o ral contraceptives ar e most used, with the male condom was the most used method by FSW (37,1%); a pproximately one fifth of the participants felt discriminated a gainst or were treated worse for being FSW and only 24.3% disclose their sex work status in heal th services. In concl usion, the data corroborate the greater vulnerability of FSW. The results provide important contributions to support local interventions. T he limitations of the study refer to the RDS method used in participants’ recruitment, and inclu des the homophily eff ect, the payment of incentives, and the geographic location of the study field which may affect the composition of networks according to th e FSW who work nearby