28492 research outputs found
Sort by
The expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells
No full textMesenchymal stromal cells (MSCs) can self-renew, differentiate into specialised cells and have different embryonic origins-ectodermal for dental pulp-derived MSCs (DPSCs) and mesodermal for adipose tissue-derived MSCs (ADSCs). Data on DPSCs adipogenic differentiation potential and timing vary, and the lack of molecular and genetic information prompted us to gain a better understanding of DPSCs adipogenic differentiation potential and gene expression profile. While DPSCs differentiated readily along osteogenic and chondrogenic pathways, after 21 days in two different types of adipogenic induction media, DPSCs cultures did not contain lipid vacuoles and had low expression levels of the adipogenic genes proliferator-activated receptor gamma (PPARG), lipoprotein lipase (LPL) and CCAAT/enhancer-binding protein alpha (CEBPA). To better understand this limitation in adipogenesis, transcriptome analysis in undifferentiated DPSCs was carried out, with the ADSC transcriptome used as a positive control. In total, 14,871 transcripts were common to DPSCs and ADSCs, some were unique (DPSCs: 471, ADSCs: 1032), and 510 were differentially expressed genes. Detailed analyses of overrepresented transcripts showed that DPSCs express genes that inhibit adipogenic differentiation, revealing the possible mechanism for their limited adipogenesis
Educação popular, equidade e saúde: dispositivos pedagógicos e práticas lúdicas de aprendizagem na saúde: a caixa de ferramentas nas relações de ensino e aprendizagem
No full textSérie Educação Popular & Saúd
The Rise of Fungi: Evidence on the Global Scale. Old Known Silences or Mysterious Threats to the Planet
No full textIntroduction: Fungi are organisms that present themselves in multicellular macroscopic and
unicellular microscopic forms. They are eukaryotic, heterotrophic, reproduce asexually/sexually,
cosmopolitan, achlorophyllates and are present in various climatic conditions and found in a variety
of species and forms in nature.
Aims: Present the emerging evolution of fungi, their underreporting, scale and parameters that
show their potential as a heterotrophic organism, decomposer and pathogen.
Methodology: In this review, we conducted a search emphasizing themes about fungi using the
available databases and based on the scientific literature, we discussed a series of recent issues
involving this wide realm and the constant controversies and expectations that guide the existence
of fungi on the planet.
Results: The results presented show an analysis of the action of these eukaryotic organisms and
their interaction with other living beings, the constant changes in taxonomy, their pathogenic
potential in plants and animals, issues related to the intrinsic resistance of some species to drugs
and also the potential biotechnological for which these organisms demonstrate high plasticity.
Conclusion: The expansion of fungal diseases to the fauna and flora of the planet; leads us to
believe that, unless measures are taken to reinforce biosafety issues, it will be increasingly
necessary to take care of the health conditions of the planet to avoid a global collapse caused by
microscopic beings
Ressignificar: vivência de uma nutricionista residente no núcleo de apoio a saúde da família
No full textEste trabalho tem como objetivo fazer aproximação com momentos da trajetória de uma nutricionista residente no Programa Integrado de Residências em Saúde da FESFSUS/ FIOCRUZ – Residência Multiprofissional em Saúde da Família, trazendo memórias, recordações, reflexões sobre a ressignificação e identidade enquanto nutricionista atuante no Núcleo de Apoio a Saúde da Família, no município de Camaçari, Bahia. O trabalho se configura enquanto memorial e apresenta as recordações e vivências mais importantes do primeiro ano de residência, dividido em sete capítulos: Descobrindo o que é ser NASF; Trabalho em família: vinculo, amizade, partilha; Paradoxo; Visita Domiciliar; Vivendo e conhecendo as práticas integrativas; Grupo de crianças da USF Phoc III; NASF é luta; E traz as vivências do segundo ano de residência em quatro capítulos: Eletivo/férias; Retorno pós eletivo/férias: peixe fora d’agua; O desafio do estágio em gestão; Estágio em redes. O processo de formação na residência multiprofissional em saúde promove qualificação da prática profissional na assistência ou docência, contrapondo-se ao modelo biológico/ biomédico, sendo um espaço de constante educação permanente, assim como contribui para o fortalecimento do SUS
Special parents and children: Narratives about the male role in health care for children with disabilities in Complexo da Maré, Rio de Janeiro
No full textLiderados por sete gestoras moradoras do Complexo da Maré - RJ, o grupo Especial da Maré debate ações e iniciativas com o enfoque nas famílias de crianças e adolescentes com necessidades especiais. Tendo como pano de fundo os altos índices de abandono paterno, principalmente no Brasil, há de se pensar como se apresenta a figura paterna dessas crianças ditas especiais, com suas diversas dificuldades, anseios, trajetórias e, principalmente, dentro das percepções masculinas sobre o cuidado em saúde e o autocuidado, ainda mais dentro de um contexto de dramas e conflitos vivenciados em um território demarcado pela violência, desigualdade e variados confrontos como o Complexo da Maré. O presente trabalho teve como objetivo, em uma abordagem de gênero, identificar as representações sobre atenção à saúde na perspectiva do papel dos homens responsáveis por crianças com deficiências, moradoras do Complexo da Maré, no Rio de Janeiro. O presente trabalho trata-se de uma pesquisa descritiva e de caráter qualitativo. Os dados obtidos através de entrevistas serão tratados através da técnica de análise de conteúdo com o uso de análise temática. A elaboração dessa proposta é que corporifique nossos desejos através de um trabalho justo entre parceiros que se coadunam com o pensamento de aproximação do público masculino aos serviços de saúde, identificando demandas, rompendo paradigmas, fornecendo subsídios para políticas públicas e evidenciando a relevância da construção de mais pesquisas sobre a temática.Led by seven managers living in the Complexo da Maré - RJ, the group Especiais da Maré debates actions and initiatives focusing on the families of children and adolescents with special needs. Against the background of the high rates of paternal abandonment, especially in Brazil, it is necessary to consider how the paternal figure of these so-called children appears, with their various difficulties, desires, trajectories and, mainly, within the male perceptions about care in health and self-care, especially within the context of dramas and conflicts experienced in a territory demarcated by violence, inequality and various confrontations such as the Complexo da Maré. The present work aimed, in a gender approach, to identify representations about health care from the perspective of the role of men responsible for children with disabilities, living in Complexo da Maré, in Rio de Janeiro. The present work is a descriptive and qualitative research. The data obtained through interviews will be treated through the technique of content analysis using thematic analysis. The elaboration of this proposal is to embody our desires through a fair work between partners that are consistent with the thought of bringing the male public closer to health services, identifying demands, breaking paradigms, and providing subsidies for public policies and highlighting the relevance of building more research on the topic
Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence
No full textConselho Nacional de Desenvolvimento Científico e Tecnológico (Brazil) within the call
“CNPq/MS/SCTIE/DECIT N° 32/2014 - Pesquisas sobre Leishmanioses” grant number reference 467389/2014-4. Institutional grants
from the Fundación Ramón Areces and Banco de Santander to the CBMSO are also acknowledged. TC received scholarship from
Fundação de Amparo a Pesquisa do Estado de Santa Catarina – FAPESC.Serodiagnosis of Leishmania infantum infection in dogs relies on the detection of antibodies
against leishmanial crude extracts or parasitic defined antigens. The expansion of canine leishmaniasis
from geographical areas of Brazil in which the infection is endemic to regions in
which the disease is emerging is occurring. This fact makes necessary the analysis of the
serodiagnostic capabilities of different leishmanial preparations in distinct geographical locations.
In this article sera from dogs infected with Leishmania and showing the clinical form of
the disease, were collected in three distinct Brazilian States and were tested against soluble
leishmanial antigens or seven parasite individual antigens produced as recombinant proteins.
We show that the recognition of soluble leishmanial antigens by sera from these animals
was influenced by the geographical location of the infected dogs. Efficacy of the diagnosis
based on this crude parasite preparation was higher in newly endemic regions when compared
with areas of high disease endemicity. We also show that the use of three of the recombinant
proteins, namely parasite surface kinetoplastid membrane protein of 11 kDa (KMP-11), and
two members of the P protein family (P2a and P0), can improve the degree of sensitivity without
adversely affecting the specificity of the diagnostic assays for canine leishmaniasis, independently
of the geographical area of residence. In addition, sera from dogs clinically healthy
but infected were also assayed with some of the antigen preparations. We demonstrate that
the use of these proteins can help to the serodiagnosis of Leishmania infected animals with sub-clinical infections. Finally, we propose a diagnostic protocol using a combination of KMP-11,
P2a y P0, together with total leishmanial extracts
Nethis Informa
No full textBoletim informativo desenvolvido pelo Nethis/Fiocruz Brasília com os principais eventos e notícias. Edição publicada no dia 09/11/2020
Severe airport sanitarian control could slow down the spreading of COVID-19 pandemics in Brazil
No full textWe investigated a likely scenario of COVID-19 spreading in Brazil through the complex airport network of the country, for the 90 days after the first national occurrence of the disease. After the confirmation of the first imported cases, the lack of a proper airport entrance control resulted in the infection spreading in a manner directly proportional to the amount of flights reaching each city, following first occurrence of the virus coming from abroad. Methodology. We developed a SIR (Susceptible-InfectedRecovered) model divided in a metapopulation structure, where cities with airports were demes connected by the number of flights. Subsequently, we further explored the role of Manaus airport for a rapid entrance of the pandemic into indigenous territories situated in remote places of the Amazon region. Results. The expansion of the SARS-CoV-2 virus between cities was fast, directly proportional to the airport closeness centrality within the Brazilian air transportation network. There was a clear pattern in the expansion of the pandemic, with a stiff exponential expansion of cases for all cities. The more an airport showed closeness centrality, the greater was its vulnerability to SARS-CoV-2. Conclusions. We discussed the weak pandemic control performance of Brazil in comparison with other tropical, developing countries, namely India and Nigeria. Finally, we proposed measures for containing virus spreading taking into consideration the scenario of high poverty
Vitamin B12 is neuroprotective in experimental pneumococcal meningitis through modulation of hippocampal DNA methylation
No full textBackground: Bacterial meningitis (BM) causes apoptotic damage to the hippocampus and homocysteine (Hcy) accumulation to neurotoxic levels in the cerebrospinal fluid of children. The Hcy pathway controls bioavailability of methyl, and its homeostasis can be modulated by vitamin B12, a cofactor of the methionine synthase enzyme. Herein, the neuroprotective potential and the underlying mode of action of vitamin B12 adjuvant therapy were assessed in an infant rat model of BM. Methods: Eleven-day old rats were intracysternally infected with Streptococcus pneumoniae serotype 3, or saline, treated with B12 or placebo, and, 24 h after infection, their hippocampi were analyzed for apoptosis in the dentate gyrus, sulfur amino acids content, global DNA methylation, transcription, and proximal promoter methylation of candidate genes. Differences between groups were compared using 2-way ANOVA followed by Bonferroni post hoc test. Correlations were tested with Spearman’s test. Results: B12 attenuated BM-induced hippocampal apoptosis in a Hcy-dependent manner (r = 0.80, P < 0.05). BM caused global DNA hypomethylation; however, B12 restored this parameter. Accordingly, B12 increased the methylation capacity of hippocampal cells from infected animals, as inferred from the ratio S-adenosylmethionine (SAM):S-adenosylhomocysteine (SAH) in infected animals. BM upregulated selected pro-inflammatory genes, and this effect was counteracted by B12, which also increased methylation of CpGs at the promoter of Ccl3 of infected animals. Conclusion: Hcy is likely to play a central role in hippocampal damage in the infant rat model of BM, and B12 shows an anti-inflammatory and neuroprotective action through methyl-dependent epigenetic mechanisms
Vivências com a intersetorialidade
No full textA abordagem do trabalho em saúde na Atenção Básica era novidade para mim, até então não estava acostumado a participar de peças de teatro, de grupo de práticas corporais, coordenar uma atividade física era para mim algo impensável. Tiv