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Resumo do Boletim InfoGripe: semana epidemiológica (SE) 07 2022
No full textSemana epidemiológica 07: 13/02/2022 a 19/02/2022.O presente boletim é referente a análises com base nos dados inseridos no Sivep-gripe até o dia 21/02/2022. Semana epidemiológica 07: 13/02/2022 à 19/02/2022. Apresentamos análises nacional, por região geopolítica, por regional baseada em perfil de atividade, e por UF, relatando o nível relativo ao plano de contingência, zona no canal endêmico e nível de atividade semanal para os dados de SRAG, SRAG por Influenza, e óbitos de SRAG por Influenza
Nested PCR for the Diagnosis of Feline Sporotrichosis From Formalin-Fixed and Paraffin-Embedded Samples Using Different DNA Extraction Protocols
No full textSporotrichosis is a chronic, cosmopolitan granulomatous mycosis that affects humans and animals. The infection is caused by the dimorphic fungi Sporothrix sp. The aims of the present study were to evaluate, standardize and validate a nested PCR technique using two DNA purification kits for the extraction of DNA from formalin fixed and paraffin-embedded tissues (FFPE) for Sporothrix sp. detection. FFPE mycological culture pellet samples of different Sporothrix species (S. chilensis, S. mexicana, S. pallida, S. globosa, S. brasiliensis and S. schenckii) were used as positive controls and clinical FFPE tissue samples of animals positive for Cryptococcus sp., Leishmania infantum and Histoplasma sp. were used as negative controls. Ten clinical FFPE skin samples from cats with sporotrichosis were used to validate the nested PCR. These samples were cut into two distinct paraffin sectioning protocols (5 and 16 μm thick). The paraffin sections were subjected to two different DNA extraction kits (chemical and thermal extractions). A nested PCR was performed on the extracted DNA to identify the genus Sporothrix. The chemical extraction protocol with the 5 μm thick paraffin section was more effective in extracting DNA from Sporothrix sp. from FFPE samples and the nested PCR technique showed the highest sensitivities (100% in the positive controls and of 50% in the skin samples of cats) and specificity (100%). Therefore, the nested PCR using this protocol has great potential to be applied in Sporothrix sp. diagnosis in FFPE samples of cats
Cluster of SARS-CoV-2 Gamma Variant Infections, Parintins, Brazil, March 2021
No full textMembers of the CDC Brazil Investigation Team: Fernanda Lessa, Danielle Fernandez, Paola Rullan-Oliver, and Antonio Vieira.High case counts after the Gamma (P. 1) variant of severe acute respiratory syndrome coronavirus 2 emerged in Brazil raised concerns that previously infected persons might become reinfected. Investigation of a cluster of coronavirus disease cases in Parintins, in the Brazilian Amazon, suggested household transmission but did not identify high rates of reinfection
Inactivated SARS-CoV-2 for safe use in analytical and non-clinical trials
No full textOs autores externos submeteram sua publicação para apresentação de trabalho no evento “International Symposium on Immunobiologicals”, que foi coordenado e organizado pelo Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), da Fundação Oswaldo Cruz
Nethis Informa
No full textBoletim informativo desenvolvido pelo Nethis/Fiocruz Brasília com os principais eventos e notícias. Edição publicada no dia 26/04/2022
Larval trematodes hosted by Biomphalaria straminea in the Brazilian semiarid region: implications for schistosomiasis control
No full textThe present study aimed to identify larval trematodes shed by snails found in water bodies
used by urban communities in a former schistosomiasis endemic area in the state of Piauí,
in the Brazilian semiarid region. A malacological survey was performed followed by analysis
of the cercariae shed by the snails after light exposure. Biomphalaria straminea specimens
(n=1,224) were obtained from all seven collection sites. Cercariae shed by snails were i)
single tailed, in which one type of cercariae was identified (Echinostoma cercariae), and ii)
with bifurcated tail (brevifurcate apharyngeate distome, brevifurcate pharyngeate distome,
and longifurcate pharyngeate distome [strigeocercaria]). Brevifurcate apharyngeate distome
were further examined and the presence of spikes in swimming membranes enabled the
identification of Spirorchiidae cercariae in all individuals, demonstrating the absence of
cercariae compatible with Schistosoma mansoni. Nevertheless, the accurate diagnosis of S.
mansoni circulation in former endemic areas is still necessary.202
Resumo do Boletim InfoGripe: semana epidemiológica (SE) 12 2022
No full textSemana epidemiológica 12: 20/03/2022 a 26/03/2022.O presente boletim é referente a análises com base nos dados inseridos no Sivep-gripe até o dia 28/03/2022. O boletim alerta que, apesar do cenário de queda nos casos de Síndrome Respiratória Aguda Grave (SRAG) em todas as faixas etárias da população adulta, crianças de 5 a 11 anos apresentaram um aumento de aproximadamente 309% na média móvel entre a primeira semana de fevereiro e a semana mais recente. Já no grupo de 0 a 4 anos, os dados apontaram um aumento de cerca de 110%. Referente à Semana Epidemiológica (SE) 12, que compreende o período de 20 a 26 de março de 2022, a investigação tem como base os dados inseridos no Sistema de Informação de Vigilância Epidemiológica da Gripe (Sivep-Gripe) até 28 de março
Virucidal activity evaluation of hydrogen peroxide wipes against the AZD1222-chimpanzee adenovirus in active pharmaceutical ingredient and the recombinant COVID-19 vaccine
No full textOs autores externos submeteram sua publicação para apresentação de trabalho no evento “International Symposium on Immunobiologicals”, que foi coordenado e organizado pelo Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), da Fundação Oswaldo Cruz
Is the mitochondrion a promising drug target in trypanosomatids?
No full textThe trypanosomatids Trypanosoma brucei, Trypanosoma cruzi and Leishmania spp. are etiological agents of important neglected tropical diseases, affecting millions of people worldwide, and the drugs available for these diseases present several limitations. Novel efficient and nontoxic drugs are necessary as an alternative to the current chemotherapy. The unique mitochondrion of trypanosomatids and its peculiar features turn this organelle a potential drug target. Several phenotypic studies describe the damage in the parasite mitochondrial ultrastructure, but the molecular target is unknown. Few reports demonstrated the electron transport system (ETS) as a target due to the high similarities to mammalian orthologues, hence ETS is not a good candidate for drug intervention. On the other hand, antioxidant enzymes, such as trypanothione reductase, and an alternative oxidase (AOX) seem to be interesting targets; however no high active inhibitors were developed up to now. Finally, due to the remarkable differences to mammalian machinery, together with the high biological importance for the parasite survival, the mitochondrial import system stands out as a very promising target in trypanosomatids. Archaic translocase of the outer membrane (ATOM) and translocase of the inner membrane (TIM) complexes, which mediate both protein and tRNA import, composed by specific subunits of these parasites, could be excellent candidates, deserving studies focused on the development of specific drugs