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Rhodnius micki, a new species of Triatominae (Hemiptera, Reduviidae) from Bolivia
No full textThis is an open access article distributed under the terms of the Creative Commons Attribution License (CC
BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Rhodnius Stål, 1859 is the second largest genus of Triatominae after Triatoma Laporte, 1832, and includes
several important Chagas vectors. Genitalia in Reduviidae are frequently used for species identification,
but the current use of terminology for it is inconsistent in Triatominae. Here, Rhodnius micki sp. nov., is
described from Bolivia and considered as belonging to the pictipes group based on its morphological char acters and distribution. Detailed documentation of the genitalia of Rhodnius micki sp. nov. is provided
with emphasis on its everted phallus, especially the endosomal sclerites, which are potentially useful as
species-level diagnostic features in Rhodnius. To further verify the validity of this species, the head shapes
and wing venation patterns of five species in Rhodnius are compared with morphometric analysis. After
reviewing taxonomic and comparative morphology papers of assassin bugs, a vocabulary with a terminol ogy of morphological characters, especially of external male genitalic characters, is assembled with the
preferred terms and the synonyms listed. Establishing a consistent terminological framework will greatly
facilitate future research on the homology of these structures across Triatominae and will ultimately con tribute to our understanding of the evolution of these groups
Immunoprophylactic Potential of a New Recombinant Leishmania infantum Antigen for Canine Visceral Leishmaniasis: An In Vitro Finding
No full textThe development and application of safe and effective immunoprophylactic/immunotherapeutic agents against canine visceral leishmaniasis (CanL) have been pointed out as the only means for the real control of the disease. Thus, this study aimed to evaluate the in vitro cellular immune response of dogs, elicited by the new recombinant proteins of Leishmania infantum, Lci10 and Lci13, in order to investigate their potential for vaccinology. Twenty-four dogs were submitted to clinical, parasitological, serological and molecular tests, and then separated into two study groups: 12 infected (InD) and 12 non-infected dogs (NInD), and six of each group were directed for Lci10 and Lci13 evaluation. Peripheral blood mononuclear cells (PBMC) were cultured and stimulated with Lci10 (10 μg/ml) or Lci13 (5 μg/ml), and with L. infantum soluble antigen (LSA) (25 μg/ml) or no stimulus (NS) as controls. Afterwards, the mRNA levels of different cytokines were quantified through qPCR, and Nitric Oxide (NO) production was assessed in the culture supernatants. Significant differences were considered when p ≤ 0.05. The comparative analysis revealed that, in the NInD group, Lci13 promoted a significant increase in the expression of IFN-γ in relation to LSA (p = 0.0362), and the expression of this cytokine in NInD was significantly higher than that presented in the InD (p = 0.0028). A negative expression for TGF-β was obtained in both groups. Lci13 also induced a greater production of NO in relation to the NS sample in the NInD group. No significant differences were observed after stimulation with Lci10. In conclusion, the results suggest a protective role of Lci13 for uninfected animals, thus with a potential for immunoprophylaxis. The results will help to direct the antigen Lci13 for further studies (pre-clinical trials), in order to determine its immunogenicity and reactogenicity effects, as a way to consolidate its real applicability for vaccinology against CanL
Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration
No full textCongenital toxoplasmosis constitutes a major cause of pre- and post-natal
complications. Fetal infection with Toxoplasma gondii influences development and can
lead to microcephaly, encephalitis, and neurological abnormalities. Few studies have attempted to explain the impact of T. gondii infection on the physiology of mature nerve
cells, and no systematic study concerning the effect of infection of neural progenitor cells
by T. gondii in the biology of these progenitors is available. We infected cortical intermediate progenitor cell cultivated as neurospheres obtained from E16.5 Swiss
Webster mice with T. gondii (Me49 strain) tachyzoites to mimic the developing mouse
cerebral cortex in vitro. Infection decreased cell proliferation as detected by Ki67 staining
at 48 and 72 hours post infection (hpi) in floating neurospheres, resulting in reduced
cellularity at 96 hpi. Neurogenic and gliogenic potential, assessed in plated
neurospheres, was shown to be impaired in infected cultures, as indicated by
neurofilament heavy chain (NF-200) and GFAP staining, respectively. To further
investigate the impact of infection on neuronal differentiation, Neuro2a neuroblasts were
infected and after 24 hpi, neurogenic differentiation was induced with serum withdrawal.
We confirmed that infection induces a decrease in neuroblast-neuron differentiation rates in cells stained for NF-200, with reduced neuritogenesis. Migration rates were analyzed
in plated neurospheres. At 120 h after plating, infected cultures exhibited decreased
overall migration rates and altered the radial migration of nestin-, GFAP- and NF-200-
positive cells. These findings indicate that T. gondii infection of neural progenitor cells
may lead to reduced neuro/gliogenesis due to an imbalance in cell proliferation alongside
an altered migratory profile. If translated to the in vivo situation, these data could explain,
in part, the cortical malformations observed in congenitally infected individuals
Práctica de la biblioterapia en Brasil y en el extranjero: algunas experiencias con la terapia de lectura
No full textAndréa Pereira Santos da Universidade Federal de Goiás, da Faculdade de Informação e Comunicação teve seu artigo submetido e aprovado na edição da RECIIS - Dossiê Feminismos: perspectivas em comunicação e informação em saúde, parte 2 v. 15 n.3 (2021)Natália Rocha da Universidade Federal de Goiás, da Faculdade de Informação e Comunicação teve seu artigo submetido e aprovado na edição da RECIIS - Dossiê Feminismos: perspectivas em comunicação e informação em saúde, parte 2 v. 15 n.3 (2021)Larissa Andrade Batista Cavalcanti da Universidade Federal de Goiás, da Faculdade de Informação e Comunicação teve seu artigo submetido e aprovado na edição da RECIIS - Dossiê Feminismos: perspectivas em comunicação e informação em saúde, parte 2 v. 15 n.3 (2021)O presente estudo tem como objetivo conhecer as principais experiências com a biblioterapia voltadas para pessoas acometidas com doenças variadas sejam físicas e/ou mentais, ou sociais no Brasil e no exterior. Justifica-se por apresentar a função terapêutica da leitura. Tem como base os conceitos teóricos e históricos da biblioterapia, o conhecimento das principais experiências com a biblioterapia no Brasil e comparativo com entre a realidade nacional e internacional quanto a utilização dessa terapia. Para tanto, trata-se de uma pesquisa exploratória, qualitativa e bibliográfica, que buscou em bases de dados nacionais e internacional (LISA) com material publicado sobre experiências que utilizassem a biblioterapia para o bem-estar do indivíduo. Desse modo, observa-se que o Brasil caminha para ampliação da utilização da terapia, enquanto que outros países a utilizam em variados nichos sejam hospitalar, escolar, prisional, entre outros. O que permite concluir que a biblioterapia proporciona saúde e bem-estar aos que dela fazem uso.This study aims to find out the main experiences with bibliotherapy aimed at people affected by various diseases, whether physical and/or mental, or social in Brazil and abroad. It is justified by presenting the therapeutic function of reading. This study is based on the theoretical and historical concepts of bibliotherapy, the knowledge of the main experiences with bibliotherapy in Brazil and comparing it with the national and international reality regarding its use. To this end, it is an exploratory, qualitative and bibliographic research, which searched for published material on national and international databases (LISA) on experiences that used bibliotherapy for the well-being of the individual. Thus, it is observed that Brazil is moving towards expanding the use of therapy, while other countries use it in hospitals, schools, prisons, among others. This allows us to conclude that bibliotherapy provides health and well-being to those who use it.Este estudio tiene como objetivo conocer las principales experiencias con biblioterapia dirigidas a personas afectadas por diversas enfermedades, físicas y/o mentales, o sociales en Brasil y en el exterior. Se justifica presentando la función terapéutica de la lectura. Este estudio se basa en los conceptos teóricos e históricos de la biblioterapia, el conocimiento de las principales experiencias con biblioterapia en Brasil y su comparación con la realidad nacional e internacional en cuanto al uso de esta terapia. Para ello, se trata de una investigación exploratoria, cualitativa y bibliográfica, que buscó material publicado en bases de datos nacionales e internacionales (LISA) sobre experiencias que utilizaron la biblioterapia para el bienestar del individuo. Así, se observa que Brasil avanza hacia la expansión del uso de la terapia, mientras que otros países la utilizan en diversos nichos, como hospital, escuela, prisión, entre otros. Esto nos permite concluir que la biblioterapia brinda salud y bienestar a quienes la utilizan
Air pollution data in COVID-19 time: A call for improving availability and accessibility
No full textSaúde Amanhã: Textos para Discussão 69: Internação de recém-nascidos de risco em Unidades de Terapia Intensiva neonatal no Brasil: uma análise espacial
No full textPublicação cujo objetivo é divulgar resultados de estudos desenvolvidos no âmbito do Projeto Saúde Amanhã, disseminando informações sobre a prospecção estratégica em saúde, em um horizonte móvel de 20 anos. Busca, ainda, estabelecer um espaço para discussões e debates entre os profissionais especializados e instituições do setor. As opiniões emitidas nesta publicação são de exclusiva e de inteira responsabilidade do autor, não exprimindo, necessariamente, o ponto de vista da Fiocruz/MS. O projeto Saúde Amanhã é conduzido pela Fundação Oswaldo Cruz (Fiocruz) no contexto da “Estratégia Fiocruz para a Agenda 2030”/Fiocruz. É permitida a reprodução deste texto e dos dados nele contidos, desde que citada a fonte. Reproduções para fins comerciais são proibidas.A redução da mortalidade neonatal segue se configurando como um grande desafio no quadro epidemiológico do país. Sua superação foi assumida pelo Brasil no contexto internacional quando o país se tornou signatário dos Objetivos de Desenvolvimento Sustentável, nos quais se ratifica o compromisso com o desenvolvimento de ações que reduzam a mortalidade neonatal, entre outras ações
In vitro and in vivo effects of P-MAPA immunomodulator on schistosomiasis
Get PDFSchistosomiasis is an infectious disease caused by helminth parasites of the genus Schistosoma; it is transmitted in over 78 countries. The main strategy for schistosomiasis control is treatment of infected people with praziquantel (PZQ). As PZQ-resistant strains have emerged, new anti-schistosomal agents have become necessary. We evaluated the in vitro and in vivo effect of P-MAPA, an aggregated polymer of protein magnesium ammonium phospholinoleate-palmitoleate anhydride with immunomodulatory properties; it is produced by Aspergillus oryzae fermentation. In vitro, P-MAPA (5, 50, and 100 µg/mL) damaged the Schistosoma mansoni tegument, causing thorn losses and tuber destruction in male worms and peeling and erosion in females after 24-h incubation. In vivo, P-MAPA (5 and 100 mg/kg, alone and combined with PZQ - 50 mg/kg) reduced the number of eggs by up to 69.20% in the liver and 88.08% in the intestine. Furthermore, granulomas were reduced up to 83.13%, and there was an increase in the number of dead eggs and a reduction of serum aspartate aminotransferase levels. These data suggest that P-MAPA activity can help improve schistosomiasis treatment and patients' quality of life
Referências e inventário de jardins históricos do campus Fiocruz Manguinhos. Separata Jardins do Pavilhão Arthur Neiva e LAFA
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Small Molecule Analysis of Extracellular Vesicles Produced by Cryptococcus gattii: Identification of a Tripeptide Controlling Cryptococcal Infection in an Invertebrate Host Model
Get PDFA composição de pequenas moléculas (massa molecular <900 Daltons) das vesículas extracelulares (EVs) produzidas pelo fungo patogênico Cryptococcus gattii é desconhecida, o que limita a compreensão das funções das EVs criptocócicas. Neste estudo, analisamos a composição de pequenas moléculas em amostras obtidas de culturas sólidas de C. gattii por uma combinação de abordagens cromatográficas e espectrométricas e metabolômica não direcionada. Esta análise revelou componentes previamente desconhecidos das EVs, incluindo pequenos peptídeos com funções biológicas conhecidas em outros modelos. Os peptídeos encontrados nas EVs de C. gattii tiveram sua estrutura química validada por abordagens químicas e comparação com padrões autênticos, e suas funções testadas em um modelo de infecção criptocócica da Galleria mellonella. Um dos peptídeos vesiculares (isoleucina-prolina-isoleucina, Ile-Pro-Ile) melhorou a sobrevivência de G. mellonella letalmente infectada com C. gattii ou C. neoformans. Esses resultados indicam que pequenas moléculas exportadas em EVs são biologicamente ativas em Cryptococcus. Nosso estudo é o primeiro a caracterizar uma molécula EV fúngica induzindo proteção, apontando para um potencial imunológico de peptídeos extracelulares produzidos por C. gattii.The small molecule (molecular mass <900 Daltons) composition of extracellular vesicles (EVs) produced by the pathogenic fungus Cryptococcus gattii is unknown, which limits the understanding of the functions of cryptococcal EVs. In this study, we analyzed the composition of small molecules in samples obtained from solid cultures of C. gattii by a combination of chromatographic and spectrometric approaches, and untargeted metabolomics. This analysis revealed previously unknown components of EVs, including small peptides with known biological functions in other models. The peptides found in C. gattii EVs had their chemical structure validated by chemical approaches and comparison with authentic standards, and their functions tested in a Galleria mellonella model of cryptococcal infection. One of the vesicular peptides (isoleucine-proline-isoleucine, Ile-Pro-Ile) improved the survival of G. mellonella lethally infected with C. gattii or C. neoformans. These results indicate that small molecules exported in EVs are biologically active in Cryptococcus. Our study is the first to characterize a fungal EV molecule inducing protection, pointing to an immunological potential of extracellular peptides produced by C. gattii