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Interaction study of CD20 and Rituximab’s scFv to propose an anti-CD20 CAR
No full textOs autores externos submeteram sua publicação para apresentação de trabalho no evento “International Symposium on Immunobiologicals”, que foi coordenado e organizado pelo Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), da Fundação Oswaldo Cruz
Feed Nasa: edição 4
No full textRevisão de conteúdo/Jornalismo: Glauber Tiburtino. Diagramação e Design Gráfico: Marcelo Santos. Realização em parceria com Ascom Cogepe
Exploring lectin-glycan interactions to combat COVID-19: Lessons acquired from other enveloped viruses
No full textThe emergence of a new human coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has imposed great pressure on the health system worldwide. The presence of glycoproteins on the viral envelope opens a wide range of possibilities for the application of lectins to address some urgent problems involved in this pandemic. In this work, we discuss the potential contributions of lectins from nonmammalian sources in the development of several fields associated with viral infections, most notably COVID-19. We review the literature on the use of nonmammalian lectins as a therapeutic approach against members of the Coronaviridae family, including recent advances in strategies of protein engineering to improve their efficacy. The applications of lectins as adjuvants for antiviral vaccines are also discussed. Finally, we present some emerging strategies employing lectins for the development of biosensors, microarrays, immunoassays and tools for purification of viruses from whole blood. Altogether, the data compiled in this review highlight the importance of structural studies aiming to improve our knowledge about the basis of glycan recognition by lectins and its repercussions in several fields, providing potential solutions for complex aspects that are emerging from different health challenges
Untargeted metabolomics insights into newborns with congenital Zika infection
No full textFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) under the grant number E-26/2002.930/2016Canadian International Development Research Centre (IDRC), grant number 108411-001Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) grant number 443875/2018-9Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) grant number 5125/2017)Zika virus (ZIKV), an emerging virus belonging to the Flaviviridae family, causes severe neurological clinical complications and has been associated with Guillain-Barré syndrome, fetal abnormalities known collectively as congenital Zika syndrome, and microcephaly. Studies have shown that ZIKV infection can alter cellular metabolism, directly affecting neural development. Brain growth requires controlled cellular metabolism, which is essential for cell proliferation and maturation. However, little is known regarding the metabolic profile of ZIKV-infected newborns and possible associations related to microcephaly. Furthering the understanding surrounding underlying mechanisms is essential to developing personalized treatments for affected individuals. Thus, metabolomics, the study of the metabolites produced by or modified in an organism, constitutes a valuable approach in the study of complex diseases. Here, 26 serum samples from ZIKV-positive newborns with or without microcephaly, as well as controls, were analyzed using an untargeted metabolomics approach involving gas chromatography–mass spectrometry (GC-MS). Significant alterations in essential and non-essential amino acids, as well as carbohydrates (including aldohexoses, such as glucose or mannose) and their derivatives (urea and pyruvic acid), were observed in the metabolic profiles analyzed. Our results provide insight into relevant metabolic processes in patients with ZIKV and microcephal
Meio de Cultura: preparo de meios de cultura
No full textdubladodubladodubladoVídeo integrante do Curso Princípios básicos do cultivo de células de mamíferos, que aborda o subtema, Meio de Cultura: Preparo de meios de culturaFiocruzMP4Tópicos em cultura de células de mamíferosPrograma de Pós-Graduação em Biociências e Biotecnologi
Evaluación del nivel de percepción de los riesgos de infección por SARSCoV-2 y de la accesibilidad a informaciones sobre la Covid-19 en Brasil
No full textMurilo Noli da Fonseca da Pontifícia Universidade Católica do Paraná, da Escola de Belas Artes, do Programa de Pós-Graduação em Gestão Urbana teve seu artigo submetido e aprovado na edição da RECIIS - Dossiê Feminismos: perspectivas em comunicação e informação em saúde, parte 1 v. 15 n.2 (2021)Larissa Maria da Silva Ferentz da Pontifícia Universidade Católica do Paraná, da Escola de Belas Artes, do Programa de Pós-Graduação em Gestão Urbana teve seu artigo submetido e aprovado na edição da RECIIS - Dossiê Feminismos: perspectivas em comunicação e informação em saúde, parte 1 v. 15 n.2 (2021)Alexandre de Fátima Cobre da Universidade Federal do Paraná, da Pró-Reitoria de Pesquisa e Pós-Graduação, do Programa de Pós-Graduação em Ciências Farmacêuticas teve seu artigo submetido e aprovado na edição da RECIIS - Dossiê Feminismos: perspectivas em comunicação e informação em saúde, parte 1 v. 15 n.2 (2021)Danilo Raul Ossufo Momade da Universidade Federal do Paraná, da Pró-Reitoria de Pesquisa e Pós-Graduação, do Programa de Pós-Graduação em Ciências Farmacêuticas teve seu artigo submetido e aprovado na edição da RECIIS - Dossiê Feminismos: perspectivas em comunicação e informação em saúde, parte 1 v. 15 n.2 (2021)Carlos Mello Garcias da Pontifícia Universidade Católica do Paraná, da Escola de Belas Artes teve seu artigo submetido e aprovado na edição da RECIIS - Dossiê Feminismos: perspectivas em comunicação e informação em saúde, parte 1 v. 15 n.2 (2021)A Covid-19 tem se revelado um problema emergencial de importância mundial. O estudo apresentado neste artigo tem como objetivo discutir a percepção e comunicação da população brasileira sobre os riscos de contrair a doença. Foi conduzido um estudo transversal envolvendo indivíduos adultos maiores de 18 anos residentes em todo o território brasileiro que responderam a um questionário eletrônico (adaptado de Betsch), entre os dias 4 e 15 de maio de 2020, sobre percepção e comunicação dos riscos de infecção pelo coronavírus. No estudo, foram incluídos 1.291 participantes, sendo em sua maioria servidores públicos (56,2%, n=725). Também constituíram maioria os pertencentes ao sexo feminino (62,9%, n=812) e os que têm nível superior (91,5%, n=1181). Já os que residem no estado do Paraná, apesar de representarem menos da metade dos entrevistados (35,9%, n=464), predominaram se comparados aos que habitam nas demais unidades federativas. Grande parte dos participantes tomou conhecimento da pandemia e obteve informações sobre ela a partir de diferentes fontes: entre elas, especialmente televisão (18,9%), sites ou portais de notícias (16,6%), mídias sociais (16,5%), jornais impressos (11,2%), profissionais de saúde (8,9%), familiares e amigos (8%) e mecanismos de pesquisa na internet (6,3%). Em relação ao grau de onfiabilidade das fontes de informação, a maioria dos participantes afirmou que tem alta confiança em informações provenientes dos profissionais de saúde (76,1%, n=982) e muitos expressaram baixa confiança em informações provenientes de folhetos (35,3%, n=456), familiares e amigos (46,7%, n=551), mídias sociais (39,7%, n=513) e telefonemas (45,1%, n=582). Com este estudo conclui-se que o nível de percepção e comunicação sobre a Covid-19 entre os que dele participaram é bom. No entanto, futuros estudos com abordagem quali-quantitativa são necessários para melhor aprofundamento do tema.The Covid-19 has been revealing a public health emergency of global importance. The study presented in this article aims to discuss the perception and communication by the Brazilian population of the risks of contracting that disease. A cross-sectional study involving adults who was at least 18 years old living in Brazilian territory was developed asking them to answer from 4 to 15 May 2020 an electronic questionnaire (adapted from Betsch) about perception and communication of the risks of coronavirus infections. A total of 1,291 participants were included in the study and the most of them were civil servants (56.2%, n=725). Female (62.9%, n=812) and people with higher education (91.5%, n=1181) also comprised more than 50% of the group. Although less than 50% of the total of participants (35.9%, n=464) were residents of the state of Paraná, they predominated when compared to the people who live in the other states. Most participants were aware of the pandemic and get news about it from different sources of information: especially television (18.9%), websites or portals of news (16.6%), social media (16.5%), newspapers (11.2%), health workforce (8.9%), family and friends (8%) and search engines (6.3%). Regarding the degree of confidence in the sources of information, the most of participants stated that they have high confidence in information given by health workforce (76.1%, n=982) and a great number of them expressed low confidence in information given by leaflets (35.3%, n=456), family and friends (46.7%, n=551), social media (39.7%, n=513) and phone (45.1%, n=582). From this study we could conclude that the level of perception and communication about Covid-19 among their participants is good. However, future studies with a qualitative and quantitative approach are necessary to achieve a profounder understanding of the matter.La Covid-19 se revela un problema de emergencia de salud pública de importancia mundial. Este artículo presenta un estudio que tiene como objetivo discutir la percepción y comunicación de la población brasileña acerca de los riesgos de contraer esa enfermedad. Ha sido realizado un estudio transversal con personas adultas mayores de 18 años residentes en todo el territorio brasileño que respondieron a un cuestionario electrónico (adaptado de Betsch), entre los días 4 y 15 de mayo, sobre percepción y comunicación de los riesgos de infección por el coronavirus. Se incluyeron en el estudio un total de 1.291 participantes, la mayoría de los cuales era de funcionarios públicos (56,2%, n=725). Mujeres también componían mayoría (62,9%, n=812) así como los que tenían estudios superiores (91,5%, n=1181). Por lo que toca a los residentes del estado de Paraná (35,9%, n=464), aunque no constituyan la mayor parte de los entrevistados, tienen un predominio si comparados con los que residen en el resto del país. Un gran número de participantes tuvieron noticia de la pandemia a través de diferentes fuentes de información: especialmente televisión (18,9%), sitios web o portales de noticias (16,6%), redes sociales (16,5%), periódicos impresos (11,2%), profesionales de la salud (8,9%), familiares y amigos (8%) y motores de búsqueda en internet (6,3%). En cuanto al grado de confianza de las fuentes de información, la mayoría de los participantes manifestó tener alta confianza en la información de los profesionales de la salud (76,1%, n=982) y muchos de ellos expresaron baja confianza en la información por medio de folletos (35,3%, n=456), familiares y amigos (46,7%, n=551), redes sociales (39,7%, n=513) y teléfono (45,1%, n=582). Ese estudio permite concluir que el nivel de percepción y comunicación sobre la Covid-19 entre los participantes es bueno. Sin embargo, son necesarios estudios futuros con un enfoque cualitativo y cuantitativo para comprender mejor el tema
Quality of raw water in the Guandu Basin of Rio de Janeiro state during water crisis of 2020
No full textNo início do ano de 2020, cerca de 9 milhões de habitantes da Região Metropolitana do
Rio de Janeiro dependente do Sistema Guandu, vivenciaram uma crise no abastecimento de
água, recebendo água com gosto e odor. Este trabalho apresenta os resultados das análises sobre
a qualidade sanitária da água bruta, captada por esse sistema, em dois momentos distintos
durante a crise hídrica, quanto à presença de cianobactérias através de estudos de
metagenômica, e de cianotoxinas na água bruta, através da quantificação de cianotoxinas,
microcistina (MC) e saxitoxina (SXT) por ELISA. Foram realizadas análises de parâmetros de
qualidade da água bruta, através da determinação dos níveis de coliformes totais e de
Escherichia coli e de alguns parâmetros físico-químicos. A água bruta captada nos dois
períodos apresentou níveis de Escherichia coli acima da faixa de valores máximo permitidos
(VMP) descritos na Resolução 357/2000 do CONAMA, assim como o valor de MC em uma
amostra de filtro. Os parâmetros físico-quimicos analisados e os níveis de SXT estavam em
conformidade com essa resolução. O gênero de cianobactéria mais abundante no período crítico
foi o Planktothricoides sp. Desta forma, é necessária a implementação do saneamento básico
nas cidades a montante do ponto de captação de água bruta desse manancial, a fim de que
eventos semelhantes aos ocorridos na crise hídrica 2020 não repitam.At the beginning of 2020, about 9 million inhabitants of the Metropolitan Region of Rio
de Janeiro, dependent on the Guandu Basin, experienced a water-supply crisis when their water
tasted badly and gave off an odor. This work presents the results of analyses of the sanitary
quality of raw water in this system at two different times during the water crisis, with respect
to the presence of cyanobacteria found using metagenomic studies, and cyanotoxins,
microcystin (MC) and saxitoxin (SXT) quantified by ELISA. Analyses of raw water quality
parameters were carried out through the determination of total coliforms and Escherichia coli
levels and some physical-chemical parameters. The raw water collected in both periods
presented levels of Escherichia coli levels above the maximum allowed values (MAV)
described in Resolution 357/2000 of the National Environmental Agency, as well as the levels
of MC in a filter sample. The physical-chemical parameters analyzed and the SXT levels were
in accordance with this resolution. The most abundant cyanobacterial genus in the critical
period was Planktothricoides sp. Therefore, it is necessary to implement basic sanitation in
cities upstream from the point of capture of raw water from this source, so that events similar
to those that occurred in the 2020 water crisis do not recur
GC-MS Characterization of Antibacterial, Antioxidant, and Antitrypanosomal Activity of Syzygium aromaticum Essential Oil and Eugenol
No full textSyzygium aromaticum has a diversity of biological activities due to the chemical compounds found in its plant products such as total phenolic compounds and flavonoids. The present work describes the chemical analysis and antimicrobial, antioxidant, and antitrypanosomal activity of the essential oil of S. aromaticum. Eugenol (53.23%) as the major compound was verified by gas chromatography-mass spectrometry. S. aromaticum essential oil was more effective against S. aureus (MIC 50 μg/mL) than eugenol (MIC 250 μg/mL). Eugenol presented higher antioxidant activity than S. aromaticum essential oil, with an EC50 of 12.66 and 78.98 µg/mL, respectively. S. aromaticum essential oil and eugenol exhibited Trypanosoma cruzi inhibitory activity, with IC50 of 28.68 ± 1.073 and 31.97 ± 1.061 μg/mL against epimastigotes and IC50 of 64.51 ± 1.658 and 45.73 ± 1.252 μg/mL against intracellular amastigotes, respectively. Both compounds presented low cytotoxicity, with S. aromaticum essential oil displaying 15.5-fold greater selectivity for the parasite than the cells. Nitrite levels in T. cruzi-stimulated cells were reduced by essential oil (47.01%; = 0.002) and eugenol (48.05%; = 0.003) treatment. The trypanocidal activity of S. aromaticum essential oil showed that it is reasonable to use it in future research in the search for new therapeutic alternatives for trypanosomiasis
Relevance of angiotensin-(1-7) and its receptor Mas in pneumonia caused by influenza virus and post-influenza pneumococcal infection
No full textResolution failure of exacerbated inflammation triggered by Influenza A virus (IAV) prevents return of pulmonary homeostasis and survival, especially when associated with secondary pneumococcal infection. Therapeutic strategies based on pro-resolving molecules have great potential against acute inflammatory diseases. Angiotensin-(1-7) [Ang-(1-7)] is a pro-resolving mediator that acts on its Mas receptor (MasR) to promote resolution of inflammation. We investigated the effects of Ang-(1-7) and the role of MasR in the context of primary IAV infection and secondary pneumococcal infection and evaluated pulmonary inflammation, virus titers and bacteria counts, and pulmonary damage. Therapeutic treatment with Ang-(1-7) decreased neutrophil recruitment, lung injury, viral load and morbidity after a primary IAV infection. Ang-(1-7) induced apoptosis of neutrophils and efferocytosis of these cells by alveolar macrophages, but had no direct effect on IAV replication in vitro. MasR-deficient (MasR-/-) mice were highly susceptible to IAV infection, displaying uncontrolled inflammation, increased viral load and greater lethality rate, as compared to WT animals. Ang-(1-7) was not protective in MasR-/- mice. Interestingly, Ang-(1-7) given during a sublethal dose of IAV infection greatly reduced morbidity associated with a subsequent S. pneumoniae infection, as seen by decrease in the magnitude of neutrophil influx, number of bacteria in the blood leading to a lower lethality. Altogether, these results show that Ang-(1-7) is highly protective against severe primary IAV infection and protects against secondary bacterial infection of the lung. These effects are MasR-dependent. Mediators of resolution of inflammation, such as Ang-(1-7), should be considered for the treatment of pulmonary viral infections
Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes
No full textsettings
Open AccessArticle
Dengue Virus Serotype 2 Intrahost Diversity in Patients with Different Clinical Outcomes
by Maria Celeste Torres 1,*OrcID,Marcos Cesar Lima de Mendonça 1,Cintia Damasceno dos Santos Rodrigues 1,Vagner Fonseca 2,3,4OrcID,Mario Sergio Ribeiro 5,Ana Paula Brandão 6,Rivaldo Venâncio da Cunha 7OrcID,Ana Isabel Dias 8,Lucy Santos Vilas Boas 8,Alvina Clara Felix 8,Maira Alves Pereira 9,Luzia Maria de Oliveira Pinto 10OrcID,Anavaj Sakuntabhai 11,†,Ana Maria Bispo de Filippis 1,† andon behalf of ZikAction Consortium 1,†
1
Laboratório de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, 21040-360 Rio de Janeiro, Brazil
2
KwaZulu-Natal Research Innovation and Sequencing Platform (KRISP), School of Laboratory Medicine and Medical Sciences, Nelson R Mandela School of Medicine, College of Health Sciences, University of KwaZulu-Natal, 4041 Durban, South Africa
3
Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil
4
Coordenação Geral dos Laboratórios de Saúde Pública/Secretaria de Vigilância em Saúde, Ministério da Saúde, (CGLAB/SVS-MS) Brasília, 70719-040 Distrito Federal, Brazil
5
Superintendência Secretaria de Vigilância em Saúde do Estado do Rio de Janeiro, 20031-142 Rio de Janeiro, Brazil
6
Laboratório Central Noel Nutels/LACEN, 20231-092 Rio de Janeiro, Brazil
7
Coordenação de Vigilância em Saúde e Laboratórios de Referência da Fundação Oswaldo Cruz, FIOCRUZ, 21040-360 Rio de Janeiro, Brazil
8
Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, 05403-000 São Paulo, Brazil
9
Fundação Ezequiel Dias/LACEN, 31630-903 Belo Horizonte, Brazil
10
Laboratório de Imunologia Viral, Instituto Oswaldo Cruz, Fiocruz, 21040-360 Rio de Janeiro, Brazil
11
Functional Genetics of Infectious Diseases, Department of Global Health, Institut Pasteur, 75015 Paris, France
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editor: Didier Musso
Viruses 2021, 13(2), 349; https://doi.org/10.3390/v13020349
Received: 27 December 2020 / Revised: 7 February 2021 / Accepted: 13 February 2021 / Published: 23 February 2021
(This article belongs to the Special Issue Endemic Arboviruses)
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Abstract
Intrahost genetic diversity is thought to facilitate arbovirus adaptation to changing environments and hosts, and it might also be linked to viral pathogenesis. Dengue virus serotype 2 (DENV-2) has circulated in Brazil since 1990 and is associated with severe disease and explosive outbreaks. Intending to shed light on the viral determinants for severe dengue pathogenesis, we sought to analyze the DENV-2 intrahost genetic diversity in 68 patient cases clinically classified as dengue fever (n = 31), dengue with warning signs (n = 19), and severe dengue (n = 18). Unlike previous DENV intrahost diversity studies whose approaches employed PCR, here we performed viral whole-genome deep sequencing from clinical samples with an amplicon-free approach, representing the real intrahost diversity scenario. Striking differences were detected in the viral population structure between the three clinical categories, which appear to be driven mainly by different infection times and selection pressures, rather than being linked with the clinical outcome itself. Diversity in the NS2B gene, however, showed to be constrained, irrespective of clinical outcome and infection time. Finally, 385 non-synonymous intrahost single-nucleotide variants located along the viral polyprotein, plus variants located in the untranslated regions, were consistently identified among the samples. Of them, 124 were exclusively or highly detected among cases with warning signs and among severe cases. However, there was no variant that by itself appeared to characterize the cases of greater severity, either due to its low intrahost frequency or the conservative effect on amino acid substitution. Although further studies are necessary to determine their real effect on viral proteins, this heightens the possibility of epistatic interactions. The present analysis represents an initial effort to correlate DENV-2 genetic diversity to its pathogenic potential and thus contribute to understanding the virus’s dynamics within its human host