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Automatic Weather Stations
No full textInformation about Automatic Weather Stations supplied by Munro.Previously curated at: http://cedadocs.ceda.ac.uk/1128/
The publish date on this item was its original published date.
Main files in this record:
Automatic_Weather_Stations.pdf
Item originally deposited with Centre for Environmental Data Analysis (CEDA) document repository by Miss Poppy Townsend. Transferred to CEDA document repository community on Zenodo on 2022-11-2
Single-Column atmospheric chemistry and meteorological Model simulations for MOSAiC
No full text<p>Here you will find a .zip file that contains the model code for the Single-Column atmospheric chemistry and meteorological Model (SCM) set up for 1-year simulations for the Multidisciplinary drifting Observatory for the Study of Arctic Climate (MOSAiC) campaign. Feel free to use the model code and output. If you have any questions, please contact Sjoerd Barten (<a href="mailto:[email protected]">[email protected]</a>) or Laurens Ganzeveld (<a href="mailto:[email protected]">[email protected]</a>).</p>
<p>The structure of the main directories is as follows:</p>
<ul>
<li>'case/': This is where the study cases are set up and run.</li>
<li>'case/input/': This is where the main input namelists are defined.</li>
<li>'case/output/': This is where you can find the model output files. (This is the place to go if you want to use the model output)</li>
<li>'work/': This is where all the routines and parameterizations of the model are located.</li>
</ul>
<p>Note that not all simulated variables are by default in the model output. This would cause extremely large data streams and slowing down of model code. If additional parameters are requested we can run these model simulation again and include these parameters. Furthermore, the SCM will not run unless input files from ECMWF ERA5 and CAMS reanalysis are provided. Due to the very large size of these files and limited storage space these are not included in the directories. These files can be downloaded through the Climate Data Store (ERA5) and Atmosphere Data Store (CAMS) or requested via email.</p>
<p>Sjoerd Barten</p>
<p><a href="mailto:[email protected]">[email protected]</a>; Wageningen University; Meteorology & Air Quality Section</p>
SlyD Accelerates trans-to-cis Prolyl Isomerization in a Mechanosignaling Protein under Load
No full textProlyl isomerization is recognized as one of the key regulatory mechanisms, which plays a crucial role in cell signaling, ion channel gating, phage virus infection, and molecular timing. This isomerization is usually slow but often accelerated by an enzyme, called peptidyl–prolyl isomerase (PPIase). In the current project, we investigate using single-molecule force spectroscopy (SMFS) the impact of a bacterial PPIase, SlyD, on the cis–trans isomerization of the proline 2225 (P2225) in an isolated 20th domain of a cytoskeletal mechanosensing protein filamin-A (FlnA20). To explore the FlnA20–PPIase interaction, we have used multiple SMFS modes, like constant velocity, constant distance, and jumping trap experiments. In our previous study, we reported the unique nature of the P2225, which is conserved in all naturally occurring filamins and can slowly (minutes) interconvert between cis–trans isomers, in absence of any PPIase. Our current results show a staggering 25-fold acceleration of the trans-to-cis isomerization rate in the presence of saturating SlyD concentration (7.25 μM) compared to the unenzymatic condition. A SlyD concentration-dependent depletion of the trans isomeric lifetime was also observed. Additionally, we observed that SlyD stabilizes the cis-isomer in the native state of FlnA20 by ∼2 kBT. This is the first single-molecule observation of the cis–trans isomerization catalysis by a PPIase in a mechanosensing protein.This work was supported by a Sonderforschungsbereich 863 A2 Grant and ZO 291/1-1 Grant from the Deutsche Forschungsgemeinschaft. M.R. acknowledges financial support through an instrument grant from the Institute for Advanced Study of Technische Universitat Munchen
Monocentric retrospective cohort of 30,423 COVID-19 patients
No full text<p><span>Population and design: </span><span>Monocentric cohort comparing COVID-19 patients exposed or not exposed to the treatment hydroxychloroquine (HCQ)+azithromycin (AZ) used as a standard of care in our center (Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Marseille, France). The inclusion period was from 2 March 2020 to 31 December 2021, with a follow-up period of 6 weeks.</span></p>
<p><span>Inclusion and exclusion criteria: </span><span>Data included were those of patients ≥ 18 years of age with PCR-proven COVID-19 disease regardless of symptoms (asymptomatic or symptomatic) and treated in our centre, i.e. having had a medical examination by one of the doctors in our centre (Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Marseille, France) either as outpatients or as inpatients, i.e. hospitalized on the day of the visit in our outpatient unit following evaluation or directly transferred from another medical ward except intensive care unit. The reasons for exclusion were an erroneous patient identification (identity surveillance and duplicates), lack of available medical data, lack of COVID-19 after checking the medical record (including patients without COVID-19 consulting for a post-COVID-19 syndrome), expression of opposition to the use of their medical data for research purposes (in accordance with European General Data Protection Regulation), and data from patients hospitalized in our center after intensive care. Data from COVID-19 outpatients left without medical advice were excluded.</span></p><p>Funding provided by: Agence Nationale de la Recherche<br>Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001665<br>Award Number: Méditerranée Infection 10-IAHU-03</p>
Sex-specific speed-accuracy tradeoffs shape neural processing of acoustic signals
No full text<p>Speed-accuracy tradeoffs – being fast at the risk of being wrong – are fundamental to many decisions and natural selection is expected to resolve these tradeoffs according to the costs and benefits of behavior. We here test the prediction that females and males should integrate information from courtship signals differently because they experience different payoffs along the speed-accuracy continuum. We fitted a neural model of decision making (a drift-diffusion model of integration to threshold) to behavioral data from the grasshopper <i>Chorthippus biguttulus</i> to determine the parameters of temporal integration of acoustic directional information used by male grasshoppers to locate receptive females. The model revealed that males had a low threshold for initiating a turning response, yet a large integration time constant enabled them to continue to gather information when cues were weak. This contrasts with parameters estimated for females of the same species when evaluating potential mates, in which response thresholds were much higher and behavior was strongly influenced by unattractive stimuli. Our results reveal differences in neural integration consistent with the sex-specific costs of mate search: Males often face competition and need to be fast. Females often pay high error costs and need to be deliberate.</p>
Purification of MBP fusion proteins using engineered DARPin affinity matrix.
No full textMaltose binding protein (MBP) has a long history as an expression tag for the production of recombinant fusion proteins. The ability of MBP to increase the solubility of fusion proteins makes it a preferred option for the expression of recombinant proteins that are unable to fold properly or that tend to form aggregates. A critical step for obtaining a sufficient amount of the MBP fusion protein is the purification. Commercially available affinity chromatography for the purification of MBP fusion proteins using amylose matrix has two main issues: (i) low (micromolar) affinity and (ii) limited number of uses due to the cleavage of polysaccharide matrix by the amylases present in the crude cell extract. To avoid this problem, we developed an affinity chromatography based on the protein-protein interaction. We use an evolved Designed Ankyrin Repeat Protein off7 (DARPin off7), which interacts with MBP with almost 1000 times higher affinity than amylose. The functionality of such affinity chromatography was tested with the purification of MBP-tagged green fluorescent protein (GFP) and flavodoxin (FLD). The optimized affinity chromatography based on the MBP-DARPin off7 interaction enables the purification of the fusion proteins in a single-step procedure. This affinity column - easy to construct, resistant to amylase and reproducible for numerous purification cycles - provides an alternative approach to commercially available affinity chromatographies for purification of proteins containing the MBP tag.This work was supported by Slovak Research and Development Agency through the project APVV-0069-15
Il chierico rosso e l'avanguardia
No full textIl percorso critico di questo libro individua il processo in evoluzione del lavoro poetico di Triperuno che, dalla rappresentazione del disordine, dello straniamento, della deformazione e del sovvertimento delle regole, giunge alla ricomposizione di un’ideologia attraverso il linguaggio.
Ne deriva una posizione assolutamente originale che, di contro all’idea ancora molto diffusa di una poesia riconducibile alla messa in scena della dissoluzione fine se stessa, sostiene la tesi dell’unitarietà. Entro questo sistema, il superamento dell’avanguardia passa attraverso la rottura e la negazione della norma linguistica, operazione di transito che non costituisce certo l’obiettivo della poetica sanguinetiana. E se la sua idea di realismo coincide con quella dell’indagine critica sulla realtà, e non con la pura mimesi del caos, la scrittura di Sanguineti intende raggiungere l’obiettivo di una filosofia della prassi che nel linguaggio e nella sua opera di sperimentazione mostri in atto la tensione sempre contraddittoria di una presa di distanza dalla sperimentazione stessa. È la ricerca di una nuova lingua che, attraversata la palus, torna ad agire sulla realtà
Data from: Awareness of residents about kala-azar and its related practices in two endemic areas of Bangladesh
No full textKala-azar, a worldwide disease, is caused by the parasitic protozoan species of genus Leishmania and transmitted by species of sandflies. Awareness of the population about the disease is essential to run a successful control-strategies program. This cross-sectional study was conducted to assess the awareness of residents about it and related practices in two highly-endemic areas of Bangladesh. In total, 511 household respondents were selected conveniently from two unions (Kushmail and Kanihari) under two sub-districts (Trishal and Fulbaria) of Mymensingh district. Interviewer-administered questionnaires were used for assessing the awareness of the respondents about kala-azar and their practices. Knowledge scores were categorized as poor (mean +1SD). Statistical tests were considered significant at p value of ≤5% (≤0.05). Chi-squared and Student's t-tests were performed for statistical analysis. The mean knowledge score of the respondents of Kanihari union regarding kala-azar was significantly higher than that of the respondents of Kushmail union (mean ±SD, 4.30 ±0.86 versus 4.12 ±0.75, p=0.002). Of the respondents, 11% and 20% had good (>5.04), 77% and 72% had average (3.43-5.04), 12% and 8% had poor (<3.43) (GAP) knowledge on kala-azar in Kushmail union and Kanihari union respectively. However, the mean knowledge score of the respondents of Kanihari union relating sandfly was significantly lower compared to that of the respondents of Kushmail union (mean ±SD, 2.49 ±0.79 versus 2.65 ±0.85, p=0.03). Of them, 22% and 19% had good (>3.39), 67% and 64% had average (3.39-1.75), and 11% and 17% had poor (<1.75) knowledge on sandfly in Kushmail union and Kanihari union respectively. More than 70% of the respondents from Kushmail and Kanihari mentioned only injection as the preferred treatment of kala-azar. Eighty-seven percent and 88% of the respondents in the two unions had chosen upazilla health complex for the treatment of their kala-azar. About 50% of the respondents in Kushmail and Kanihari liked to use coil or mat as a preventive measure to avoid sandfly bites. The findings of this study indicate the importance of awareness and related practices of the community people in two endemic areas that will help implement the kala-azar-elimination program successfully in Bangladesh.<div class="o-metadata__file-usage-entry">data<div class="o-metadata__file-name"></div></div>
