399 research outputs found
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The Modular μSiM: insituPerm_Coated_ImageJ Analysis_2021-07-08.xlsx
No full textin situ permeability raw data (extracted fluorescence intensity values) for The Modular μSiM: A Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro</p
The Modular μSiM: insituPerm_Coated_ImageJ Analysis_2021-06-28.xlsx
No full textin situ permeability raw data (extracted fluorescence intensity values) for The Modular μSiM: A Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro</p
The Modular μSiM: insituPerm_Uncoated_ImageJ Analysis_2021-02-17.xlsx
No full textin situ permeability raw data (extracted fluorescence intensity values) for The Modular μSiM: A Mass Produced, Rapidly Assembled, and Reconfigurable Platform for the Study of Barrier Tissue Models In Vitro</p
Promoting Innovations in Education: Stories and Practices of Entrepreneurial Educators
No full textWhat do educational innovations look like, and what can they accomplish? This e-book addresses educational innovations using entrepreneurship as a lens. It examines how a few educators have promoted and carried out innovations that added value to their organizations and the clients they served, and by doing so contributed solutions to specific challenges in education.</p
Mastering Your NIH Data Management and Sharing Plan, September 11, 2025
No full textThis webinar focuses on educating potential NIH grantees on how to successfully and efficiently produce a data management and sharing plan.Description of event: Are you ready to meet the NIH's standards for data management and sharing? Do you understand the new public access policy? Our workshop will give you an overview of the NIH Policy for Data Management and Sharing, guide you through the steps to effectively create a Data Management and Sharing Plan (DMSP), and give an overview of the 2025 NIH Public Access Policy. From data management strategies to selecting the right repository, our experts will provide the tools and guidance you need to succeed. Don't miss this opportunity to ensure your research meets the NIH's high standards for data sharing.This webinar was originally presented via Zoom on September 11, 2025. Captions have not yet been added to this recording, but we are in process of adding captions to this recording and will update this record once the captions have been added. This webinar is part of the UR-Specific NIH Data Management and Sharing Resources collection. </p
Mastering Your NIH Data Management and Sharing Plan, May 13, 2025
No full textThis webinar focuses on educating potential NIH grantees on how to successfully and efficiently produce a data management and sharing plan.Description from author: Are you ready to meet the NIH's standards for data management and sharing? Do you understand the new public access policy? Our workshop will give you an overview of the NIH Policy for Data Management and Sharing, guide you through the steps to effectively create a Data Management and Sharing Plan (DMSP), and give an overview of the 2025 NIH Public Access Policy. From data management strategies to selecting the right repository, our experts will provide the tools and guidance you need to succeed. Don't miss this opportunity to ensure your research meets the NIH's high standards for data sharing.This webinar was originally presented via Zoom on May 13, 2025.</p
Cell Reports Methods Paper Codes
No full textThis repository contains the R code used to systematically benchmark ten Seurat-implemented differential gene-expression (DGE) methods for scRNA-seq data and to provide guidance on selecting appropriate DGE methods.</p
Simulating Axion-Like Particle Detection from Core-Collapse Supernovae in the IceCube Neutrino Observatory
No full textThis research project simulates the detection of axion-like particles (ALPs) from core-collapse supernovae (CCSN) in the IceCube Neutrino Observatory. We model gamma-ray production from ALP interactions with proton and oxygen nuclei in the ice, and then use GEANT4 software to simulate pair production and Compton scattering processes that generate energetic leptons. The resulting Cherenkov radiation is then analyzed using the IceCube Asteria Python package to model how these signals would be detected by IceCube.</p
Protective Role of <i>Bifidobacterium longum ssp. infantis</i> in Allergic Response
No full textBackground: Food Allergy (FA) is a critical and potentially life-threatening1 condition that affects ∼8% of US children.2 Despite growing financial3 and social burdens4 attributed to pediatric FA, mechanisms underlying the increased prevalence of FA remain poorly understood. Here, we expand on current knowledge regarding FA by examining the role of Bifidobacterium longum ssp. infantis (B. infantis) in immune response.Objectives: Caco-2 cell monolayers were treated with B. infantis supernatant and Lipopolysaccharide (LPS) purified from E. coli 0111:B4 to 1) quantify the resulting intestinal permeability, 2) visualize the subsequent formation of tight junctions, and 3) analyze the innate immunologic activity of B. infantis.Methods: Standard cell-line maintenance and subculturing protocols were used to grow Caco-2 cell monolayers. Transwell protocols were applied to treat cells with B. infantis supernatant and LPS. Trans Epithelial Electric Resistance (TEER) was used to measure the confluency of epithelial cells and determine intestinal permeability. Tight junctions were visualized using a Keyence microscope after immunofluorescence (IF) staining. RT-qPCR was run to analyze the expression of IL-8 cytokine.Results: There was no significant difference between TEER values before and after Caco-2 cell monolayers were incubated for 4 hours with treatment conditions. Fluorescence microscopy showed that cells treated without LPS were generally larger than those treated with LPS. Monolayers treated with LPS demonstrated regions of disrupted cell proliferation. All treatment conditions significantly lowered LPS-induced IL-8 expression. B. infantis strains 3 and 5 had a comparable effect on decreasing IL-8 expression.</p