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호산구 천식 마우스 모델에서 Bruton’s Tyrosine Kinase (BTK) 억제제의 효과
No full textMasterⅠ. INSTRUCTION 1
Ⅱ. MATERIALS AND METHODS 4
A. Animals 4
B. Establishment of eosinophil mouse model and ibrutinib treatment 4
C. Evaluation of airway resistance, cytokines in bronchoalveolar lavage fluid, and lung histology 5
D. Measurements of immunoglobulin levels 7
E. The assessment of cell viability by CCK-8 assay 7
F. Cell culture 8
G. Cell treatment and measurement 8
H. The isolation of mouse mononuclear cells 9
I. Detection BTK signaling pathway of western-blotting 10
J. Antibodies and reagents 11
K. Statistical analysis 12
Ⅲ. RESULTS 13
A. The effect of ibrutinib on airway hyperresponsiveness and inflammation in a mouse model of asthma 13
B. The effect of ibrutinib on the OVA specific antibody production 16
C. The ex vivo effect of ibrutinib in splenic mononuclear cells from asthmatic mice 17
D. The effect of ibrutinib in T cells 19
E. The effect of ibrutinib on IgE-sensitized mast cells 20
F. The effect of ibrutinib in eosinophils 22
G. The inhibitory effect of ibrutinib in the protein expressions of BTK signaling pathway in the mouse lung tissues 23
Ⅳ. DISCUSSION 25
Ⅴ. CONCLUSION 32
REFERENCES 33
국문요약 3
Monocyte Count and Systemic Immune-Inflammation Index Score as Predictors of Delayed Cerebral Ischemia after Aneurysmal Subarachnoid Hemorrhage
No full textMasterI. INTRODUCTION 1
II. METHODS 3
A. Study Design and Patient population 3
B. Demographics and Clinical Parameters 3
C. Laboratory Parameters 4
D. Definition and Assessment of Delayed Cerebral Ischemia 4
E. Statistical Analyses 4
III. RESULTS 6
IV. DISCUSSION 8
V. CONCLUSIONS 11
VI. REFERENCES 20
VII. STATEMENTS AND DECLARATION 2
플라즈마 활성수를 첨가한 폴리우레탄 드레싱 제제의 건조 후 살균력 지속 효과 특성 분석
No full textMasterI. 서론 1
제 1 절 연구 배경 1
제 2 절 연구 목적 6
II. 연구 방법 7
제 1 절 연구재료와 방법 7
1. 실험재료 7
2. 실험방법 12
Ⅲ. 연구 결과 16
제 1 절 보관 온도변화에 따른 PAW 의 살균력 지속성 실험 16
제 2 절 시간 경과에 따른 PAW 살균력 지속성 실험 17
제 3 절 PAW 농도의 변화와 시간 경과에 따른 살균력 지속성 실험 19
제 4 절 PAW 농도 변화에 따른 24 시간 경과 후 살균력 실험 20
제 5 절 PAW 농도 변화에 따른 60℃ 24 시간 경과 후 살균력과 폴리우레탄 제제의 표면변화 22
제 6 절 온도변화와 시간 경과에 따른 폴리우레탄 제제의 표면변화 25
Ⅳ. 고찰 27
ABSTRACT 35
참고문헌 3
혈액투석환자의 e헬스리터러시와 자가관리의 관계 : 자기효능감, 사회적 지지, 사회적 고립의 매개작용
No full textMasterⅠ. 서론 1
1. 연구의 필요성 1
2. 연구목적 7
3. 용어정의 7
Ⅱ. 문헌고찰 9
Ⅲ. 연구방법 16
1. 연구설계 16
2. 연구대상자 및 표본크기 16
3. 자료수집과정 17
4. 측정도구 18
5. 분석방법 21
Ⅳ. 연구결과 22
Ⅴ. 논의 36
Ⅵ. 결론 42
참고문헌 44
부록 63
영문초록 8
응급실 간호사의 재난간호역량에 미치는 영향 요인
No full textMasterⅠ. 서론 1
A. 연구의 필요성 1
B. 연구목적 4
C. 용어정의 5
Ⅱ. 문헌고찰 8
A. 재난간호역량 8
B. 재난간호역량 관련요인 11
Ⅲ. 연구방법 20
A. 연구설계 20
B. 연구대상 20
C. 연구도구 21
D. 자료수집방법 24
E. 자료분석방법 24
F. 윤리적고려 25
Ⅳ. 연구결과 26
A. 대상자의 일반적 특성 26
B. 재난경험, 재난준비도, 자기효능감, 의사소통능력 및 재난간호역량 28
C. 일반적 특성에 따른 재난간호역량의 차이 30
D. 재난간호역량, 재난경험, 재난준비도, 자기효능감과 의사소통능력 간의 관계 32
E. 재난간호역량에 미치는 영향 요인 34
Ⅴ. 논의 36
Ⅵ. 결론 및 제언 42
A. 결론 42
B. 제언 43
참고문헌 44
부 록 54
ABSTRACT 5
Development of a Transient Shuttle-Based Probe for Chronic Monitoring of Wide Brain Areas
No full textMasterⅠ. INTRODUCTION 1
A. Monitoring methods of electrical brain activity 1
B. Evolution and challenges of MEAs 3
C. Enhancing chronic brain implants: Advances in flexible mesh probes 5
D. Soluble materials for deep brain implantation of flexible electronics 7
E. Aims 8
Ⅱ. MATERIALS AND METHODS 10
A. Procedure for fabricating the transient shuttle-based probe 10
B. Decreased rigidity of the transient shuttle-based probe 13
C. Brain tissue phantom using agarose gel 14
D. In vivo test using mice 14
E. Micro-CT imaging 15
F. Analysis method for Micro-CT Image 16
G. Tissue preparation and immunostaining 18
H. Analysis of immunostaining image 19
Ⅲ. RESULTS 21
A. Concept for the research and fabrication methods. 21
B. Mechanical characteristics of transient shuttle-based probes during insertion 32
C. Mechanical characteristics of transient shuttle-based probes during withdrawal. 39
D. In vivo test using mouse brains for insertion and withdrawal. 51
E. Histology studies of chronic implantation of the transient shuttle-based probe in mouse brains. 60
Ⅳ. CONCLUSIONS 69
REFERENCES 71
국문 요약 7
Risk Factors for Rapid Cognitive Decline in Amyloid-Negative Individuals Without Cognitive Impairment or With Early-Stage Cognitive Loss in Screening Tests
No full textPurpose: Although rapid cognitive decline (RCD) is an important unfavorable prognostic factor, not much is known about it, especially in amyloid-negative individuals. The purpose of this study was to investigate risk factors for RCD in amyloid-negative individuals. Patients and Methods: We retrospectively enrolled 741 individuals who were either cognitively unimpaired or had early-stage cognitive ability loss and who underwent 18F-florbetaben (FBB) (n = 402) or 18F-flutemetamol (FMM) (n = 339) PET/CT. Based on visual and semiquantitative (SUV ratio [SUVR]-based) analysis, the following amyloid-negative groups were established: visual-negative FBB (n = 232), visual-negative FMM (n = 161), SUVR-negative FBB (n = 104), and SUVR-negative FMM (n = 101). Univariable and multivariable logistic regression analyses were performed for RCD using 5 SUVRs, 5 cortical thicknesses, and 5 neuropsychological domains and clinico-demographic factors. Results: In the amyloid-negative groups, a decline in language function was commonly identified as a significant risk factor for RCD (P = 0.0044 in the visual-negative FBB group, P = 0.0487 in the visual-negative FMM group, P = 0.0031 in the SUVR-negative FBB group, and P = 0.0030 in the SUVR-negative FMM group). In addition, declines in frontal/executive function, frontal SUVR, and parietal SUVR; a longer duration of education; and mild cognitive decline in the amyloid-negative groups were also significant risk factors for RCD. Conclusions: Even in amyloid-negative individuals without cognitive impairment or with early-stage cognitive ability loss, those with decreased language and frontal/executive functions on neuropsychological testing are at risk of progression to RCD
Multiple burr hole and erythropoietin combination therapy: optimal early surgical intervention for patients with acute stroke episode of moyamoya disease or moyamoya syndrome
No full textObjective: The optimal timing of bypass surgery for patients with moyamoya disease (MMD) or moyamoya syndrome (MMS) following an acute stroke episode remains unclear, mainly owing to the risk of postoperative complications. In this study, we aim to validate the safety and efficacy of early intervention using multiple burr hole (MBH) and erythropoietin (EPO) therapy, thereby refining the management strategy for patients with acute stroke episode of MMD or MMS. Methods: We retrospectively analyzed data from 70 patients with MMD or MMS who underwent MBH and EPO therapy. The cohort was divided based on the time interval between the latest neurological deterioration and surgery: early (<30 days) and later (≥30 days) groups. We evaluated and compared perioperative clinical parameters and the extent of neovascularization on a 6-month postoperative angiography. Long-term clinical outcomes, including transient ischemic attack (TIA), infarction, hemorrhage, and seizure, were also analyzed during the follow-up period. Results: In the cohort, 36 patients (51.4%) were in the early group, whereas 34 (48.6%) were in the later group. The 6-month follow-up angiography demonstrated that 34/47 hemispheres (72.3%) in the early group exhibited successful neovascularization (≥2/3 of MCA territories) compared with the 19/44 (43.2%) hemispheres in the later group (odds ratio [OR] = 3.44; 95% confidence interval [CI]: 1.46–8.45; p < 0.01). In addition, a notable reduction (≥50%) in basal moyamoya vessels was observed in 30/47 hemispheres (63.8%) from the early group vs. 12/44 (27.3%) hemispheres from the later group (OR = 4.71; 95% CI: 1.97–11.82; p < 0.001). During the average follow-up of 56.5 months, only six patients experienced infarction or hemorrhage. Conclusion: Our dataset suggests that MBH and EPO combination therapy is an effective, minimally invasive, and acceptable treatment, even in the early period of patients with MMD or MMS following an acute stroke episode
두경부암 마우스 모델을 이용한 Metformin의 항암 효과 및 암 미세환경 연구
No full textMasterⅠ. 서 론 1
Ⅱ. 재료 및 방법 3
1. Antibodies & Reagents 3
2. Cell Culture 4
3. Cell Viability Assay 4
4. In vivo Study 5
5. Immunohistochemistry 5
6. Statistical Analysis 6
Ⅲ. 결 과 7
A. Metformin 의 처리는 시간과 농도에 의존하여 MOC1,2,22 세포에서 세포 생존도를 감소시킨다. 7
B. MOC2 종양 모델은 MOC1 종양 모델보다 더 공격적이고 전이가 활발한 모델이다. 9
C. Metformin 은 MOC2 종양 보다는 MOC1 종양 의 성장을 늦춘다. 11
D. Metformin 의 처리는 MOC1 종양의 암 미세환경을 정상의 상태로 되돌린다. 12
E. MOC1 종양은 MOC2 종양에 비해 면역 활동이 활발한 종양이다. 13
Ⅵ. 고 찰 33
Ⅴ. 참고 문헌 37
Abstract 4
Effect of a Logotherapy-Based Empowerment Program for Achieving Self-Reliance Among Persons Living Houseless
No full textThe lives of houseless individuals are characterized by powerlessness, meaninglessness, hopelessness, and despair, yet they have a desire to escape homelessness. While the economic aspect is essential for escaping homelessness, psychological resources are crucial, as they form the basis of the strength needed to achieve independence. The study aimed to examine the effectiveness of a logotherapy-based empowerment program developed to strengthen the capabilities of persons living houseless and ultimately develop competencies that serve as a foundation for self-reliance. The study was performed in a homeless support center for men living houseless in South Korea using a repeated-measures design with a control group. The experimental group received an eight-session empowerment program over 8 weeks. The participants were assessed at three intervals: pretest, posttest immediately after the program, and follow-up test 4 weeks after the posttest. The follow-up tests were completed by 22 and 16 participants in the experimental and control groups, respectively. We found that the empowerment program significantly enhanced the meaning of life, hope, and empowerment of houseless individuals. Community mental health nurses, who are in a position to interact with persons living houseless, must empower them to escape homelessness and achieve self-reliance, an important goal for this population