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Temporal Dynamics and Treatment Outcomes of Hepatitis C Virus/Human Immunodeficiency Virus Coinfection: A Multicenter Retrospective Study from South Korea
No full textBACKGROUND/AIMS: Due to the very low incidence of human immunodeficiency virus (HIV) infection in South Korea, epidemiological data on hepatitis C virus (HCV)/HIV coinfection are limited. The aim of this study was to investigate the clinical characteristics and treatment outcomes of patients with HCV/HIV coinfection in South Korea. METHODS: We retrospectively collected data from patients diagnosed with HCV/HIV coinfection at 12 academic hospitals in South Korea from 2009 to 2020. RESULTS: A total of 124 patients were included in this study; most patients were males (n=112, 90.3%), and the mean age was 46.5+/-13.5 years. Among the study patients, 11 (8.9%) had cirrhosis, and seven (5.6%) tested positive for the hepatitis B surface antigen. During the follow-up period (mean period: 67.4 months), two patients (1.6%) developed hepatocellular carcinoma, and nine (7.3%) died. Of the 112 patients (90.3%) who underwent HCV genotype testing, most were infected with HCV genotype 2 (n=53, 47.3%) and genotype 1b (n=41, 36.6%). In particular, HCV genotype 1a was identified in 12.5% (n=14) of patients. Ninety-one patients (73.4%) received antiviral therapy, with 104 antiviral treatments administered overall. The sustained virologic response rate was significantly higher in patients treated with direct-acting antiviral agents (DAA) than in those receiving pegylated interferon-based treatment (89.0% vs 58.1%, p<0.001). CONCLUSIONS: In South Korea, patients with HCV/HIV coinfection were predominantly male and younger and exhibited a higher prevalence of genotype 1a than those with HCV monoinfection. These patients demonstrated a significantly better treatment response to DAA treatment than to interferon-based therapy
Real-world clinical response and efficacy of tacrolimus-based maintenance therapy for Korean patients with lupus nephritis
No full textBACKGROUND/AIMS: We evaluated the efficacy and safety of tacrolimus as maintenance therapy in Korean patients with lupus nephritis (LN). METHODS: A total of 179 patients with biopsy-proven LN were included, of whom 92 received tacrolimus and 87 did not. Clinical parameters were assessed at six months and at one, two, three, and five years. Complete renal response (CR) and partial response (PR) were defined based on established criteria. Adverse events, renal flares, and poor outcomes have been reported. RESULTS: Baseline characteristics were similar, except for a higher prevalence of class V LN in the tacrolimus group. At six months, the CR rate was 49.5% in the tacrolimus group and 56.6% in the non-tacrolimus group (p = 0.308), with PR rates of 33.0% and 24.1% (p = 0.213). At one year, the non-tacrolimus group had a significantly higher CR rate (73.1% vs. 52.3%, p = 0.006), whereas the overall response rates were similar (p = 0.15). By two years, the CR rates were 71.8% in the non-tacrolimus group and 58.2% in the tacrolimus group (p = 0.031). At three years, the overall response was found 75.4% with tacrolimus and 83.1% without (p = 0.252); and at five years, these rates were 72.9% and 87.3% (p = 0.1). No significant differences in renal flares, poor outcomes, or adverse events were observed. CONCLUSION: This study has demonstrated that tacrolimus is an effective and safe maintenance therapy for achieving renal response and slowing disease progression in patients with LN who have not achieved remission
진행성 폐암 환자의 건강관련 삶의 질 구조모형
No full textDoctorI. 서론 1
A. 연구의 필요성 1
B. 연구의 목적 4
C. 용어의 정의 5
II. 문헌고찰 9
A. Wilson 과 Cleary 의 건강관련 삶의 질 모델 9
B. 진행성 폐암 환자의 건강관련 삶의 질 19
C. 진행성 폐암 환자의 건강관련 삶의 질 관련요인 22
III. 개념적 기틀 33
A. 본 연구의 개념적 기틀 33
B. 가설적 모형 37
C. 연구가설 39
IV. 연구방법 40
A. 연구설계 40
B. 연구대상 40
C. 연구도구 42
D. 자료수집방법 46
E. 윤리적 고려 47
F. 자료분석방법 48
V. 연구결과 50
A. 대상자의 일반적 특성 50
B. 대상자의 질병 관련 특성 52
C. 측정도구의 신뢰도 54
D. 측정변수의 정규성 검정 56
E. 잠재변수 간의 다중공선성과 상관관계 58
F. 측정모형의 타당성 검증 61
G. 구조모형의 검증 65
VI. 논의 76
A. 진행성 폐암 환자의 건강관련 삶의 질 정도 76
B. 진행성 폐암 환자의 주요 내생변수별 영향요인 78
C. 연구의 제한점 88
D. 연구의 의의 89
VII. 결론 및 제언 91
A. 결론 91
B. 제언 92
참고문헌 93
부록 109
ABSTRACT 12
중등학교 교사의 정신건강에 영향을 미치는 요인
No full textMasterⅠ. 서론 1
A. 연구의 필요성 1
B. 연구의 목적 4
C. 용어의 정의 5
Ⅱ. 문헌고찰 7
A. 교사의 정신건강 7
B. 교사의 감정부조화와 정신건강 10
C. 교사의 사회적지지와 정신건강 13
D. 교사의 직무만족과 정신건강 15
Ⅲ. 연구방법 18
A. 연구 설계 18
B. 연구 대상 18
C. 연구 도구 20
1. 정신건강 20
2. 감정부조화 20
3. 사회적지지 21
4. 직무만족 21
D. 자료 수집 절차 22
E. 윤리적 고려 22
F. 자료 분석 방법 23
Ⅳ. 연구결과 24
A. 연구 대상자의 일반적 특성 24
B. 연구 대상자의 감정부조화, 사회적지지, 직무만족, 정신건강 정도 26
C. 연구 대상자의 일반적 특성에 따른 정신건강의 차이 28
D. 연구 대상자의 정신건강과 제 변인과의 관계 29
E. 연구 대상자의 정신건강에 영향을 미치는 요인 30
Ⅴ. 논의 32
Ⅵ. 결론 및 제언 39
A. 결론 39
B. 제언 40
참고문헌 41
부 록 52
ABSTRACT 6
유전체 안정성 조절기전에서 LC3B 의 기능 규명
No full textDoctorⅠ. INTRODUCTION 1
1. Oligodendrocytes and the oligodendrocyte lineage 1
2. Experimental models of oligodendrocytes and related diseases 3
3. Development of experimental models for research on oligodendrocytes and ischemic white matter injury 4
Ⅱ. MATERIALS & METHODS 6
1. Animals 6
2. The E3 method for primary OL culture 6
3. Primary OL culture (shaking method) 10
4. Cryopreservation of primary OPCs (E3 method) 10
5. EdU proliferation assay 11
6. WST-8 cell viability and proliferation assay 11
7. Genetic manipulation 12
8. One-step myelinating OL/neuron co-culture 13
9. Oxygen-glucose deprivation (OGD) 13
10. Cell death assays 14
11. In vivo hypoxia 14
12. Spectral Confocal Reflectance (SCoRe) microscopy 15
13. Immunofluorescence 15
14. Immunoblotting 16
15. Image analyses 16
16. Statistical analysis 18
Ⅲ. RESULTS 19
Part I: Development of the E3(Easy, Efficient, Effective) method for primary oligodendrocyte culture from neonatal rodent brains. 19
1. The necessity for the de novo development of a primary OL culture method 19
2. Trials with density gradient centrifugation and the discovery of a two-step differential centrifugation to isolate OPCs 27
3. Optimization of OPC proliferation media formulae 31
4. Cell death during OL differentiation and the need for the passaging of crowded OPC colonies 36
5. Optimization of OL differentiation media formulae 46
6. Applicability of the E3 method to C57BL/6 mice 51
7. In vitro myelination of OLs cultured through the E3 method 53
Part II: Cryopreservation of primary OLs, and spatial/morphological analyses to evaluate in vitro OL function. 56
1. Cryopreservation of primary OLs cultured through the E3 method 56
2. In vitro functional validity of cryopreserved OLs 60
3. Poisson point process-based evaluation of the spatial distribution of OPCs 65
4. Sholl analysis and fractal dimensionality analysis to assess the morphological complexity of mature OLs 68
Part III: Adjunctive experimental methods for in vitro primary OLs. 71
1. Genetic manipulation of primary rat OPCs 71
2. Modification of the E3 method for adult rodent brains 75
3. One-step neuron-OL myelinating co-culture 77
4. The role of osmolarity in OPC proliferation and OL differentiation 81
Part IV: In vitro and in vivo experimental models of ischemic white matter injury. 83
1. In vitro oxygen-glucose deprivation (OGD) 83
2. Cell death and damage assays to evaluate OL injury after OGD 88
3. In vivo rodent model of neonatal hypoxic-ischemic encephalopathy (HIE) 91
4. Label-free detection of myelin and myelin damage 93
Ⅳ. DISCUSSION 95
Ⅴ. REFERENCES 10
SARS CoV-2 변종 및 INFLUENZA A 바이러스 하위 유형에 대한 유사 유형 바이러스 기반 중화 항체 테스트
No full textMasterI. INTRODUCTION 1
II. MATERIALS AND METHODS 6
1. Vector Constructions 6
2. Cell Lines, Antibodies and Reagents 8
3. Preparation Of Vaccinia Virus-T7 (vTF7-3) Stock for Vaccinia Virus Plaque- forming Assay 8
4. Generation of ΔG-VSV -based (ΔG-VSV /NanoLuc)-SARS-CoV-2pseudotype particles 9
5. Generation of single cycle (sc)VSVΔG-H1N1/H3N2 13
6. Nanoparticle tracking analysis 15
7. Electron Microscopy 15
8. Confocal Microscopy 15
9. SDS PAGE and Immunoblotting 17
10. Determination of TCID50 17
11. Immunization of mice and Sample Collection 18
12. Neutralization antibody Assays 18
13. Statistical Analysis 19
III. RESULTS 20
1. Vaccinia Virus Titration through Plaque Assay 20
2. Production of Pseudo-typed VSV viruses 20
3. Characterization of Spike-Pseudotyped ΔG-VSV /Nanoluc and Single Cycle- ΔG-VSV H1N1/H3N2 Pseudotyped Virus 21
4. Infectivity Kinetics and Tissue Culture Infectious Dose (TCID50) Analysis for G*ΔG-VSV and S*ΔG-VSV 25
5. Construction and Characterization of EVs-Based Vaccine Candidate Expressing Four Structural Proteins of SARS-Cov-2 30
6. Neutralization Antibody Response of EVs Vaccine Candidate Loaded With SARS-CoV-2-Structural Proteins in Mice Model 30
7. Infectivity Kinetics and Tissue Culture Infectious Dose (TCID50) Analysis of Single Cycle (sc) VSVΔG-H1N1/H3N2 Nanoluc Virus Particle 34
DISCUSSION 36
LIMITATIONS 39
CONCLUSION 39
REFERENCES 40
국문요약 4
Screening of Senolytics Targeting Senescent Melanocytes
No full textMasterⅠ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 4
1. Cell Culture 4
2. In Vitro model of UVB-induced Senescent Melanocyte (UVSM) 4
3. Materials 4
4. Senescence-Associated β-Galactosidase (SA-β-Gal) Staining 5
5. Real-Time PCR Analysis 5
6. MTT Assay 5
7. Cell Counting 6
8. Western Blot Analysis 6
9. Measurement of Caspase-3/7 Activity 6
10. PE Annexin V/7-AAD Analysis 7
11. Statistical Analysis 7
Ⅲ. RESULTS 8
1. Evaluation of senolytic drugs on senescent melanocytes 8
2. ABT-737 and ABT-263 eliminated senescent melanocytes 21
3. ABT-737 and ABT-263 induced caspase activity 24
4. Caspase inhibitors restore apoptosis in senescent melanocytes 27
Ⅳ. DISCUSSION 31
Ⅴ. REFERENCES 33
Ⅵ. 국문 요약 3
Loss of NAMPT in intestinal epithelium promotes colitis-associated colorectal cancer development
No full textMasterI. INTRODUCTION 1
II. MATERIALS AND METHODS 3
A. Mice 3
B. Histology and Immunofluorescence 3
C. Isolation of colon lamina propria cells 4
D. Flow cytometric analysis 4
E. Measurement of NAD 5
F. Western blotting 5
G. Quantitative RT-PCR 5
H. AOM-induced murine colon cancer 6
I. Exome sequencing 6
III.RESULTS 8
A.Establishment of gut epithelium-specific NAMPT knockout mouse. 8
B. Deletion of NAMPT in the intestinal epithelium leads to severe colitis. 11
C. NMN administration rescues NAMPT deficiency-induced colitis. 14
D.NAMPT deficiency in intestinal epithelium leads to colon cancer. 19
E.NAMPT∆IEC mice are more susceptible to AOM 30
F.Early-life colitis plays a critical role in the development of colitis-associated colorectal cancer. 36
IV. DISCUSSION 39
V. REFERENCES 42
국문요약 4
뇌졸중 후 전기 자극을 통한 혈관 신생 촉진 및 전자약 파라미터 개발
No full textMasterI. 서론 1
II. 실험 방법 3
A. Cell culture 3
B. Primary rat brain endothelial cell culture 3
C. Electrical stimulation 4
D. Migration assay 5
E. Cell proliferation assay 5
F. Tube formation assay 6
G. Immunocytochemistry 6
H. Photothrombosis stroke model 7
III. 실험 결과 8
1. 전기 자극 매개 변수 설정 8
2. 40Hz 전기 자극이 혈액-뇌 장벽 관련 단백질에 미치는 영향 10
3. 전기 자극이 내피세포의 이동성에 미치는 영향 13
4. 전기 자극이 내피세포의 증식에 미치는 영향 16
5. Tube formation assay에서의 배지 조성 19
6. Tube formation assay에서의 적절한 HUVECs 밀도 21
7. 전기 자극이 내피세포의 혈관 신생에 미치는 영향 23
8. 전기 자극이 내피세포의 혈액-뇌 장벽 관련 단백질 발현에 미치는 영향 26
9. Primary endothelial cell에서 전기 자극에 따른 최적 배양 조건 확인 29
10. Primary endothelial cell culture 이후 전기 자극 줬을 때 차이 32
11. Photothrombosis stroke model 제작 34
IV. 논의 및 결론 36
V. 참고 문헌 42
Abstract 4
중·고령자의 경제활동과 건강노화의 종단적 연관성
No full textDoctor제 1장 서 론 1
제 1절 연구배경 1
제 2절 연구목적 및 연구문제 4
1. 연구목적 4
2. 연구문제 5
제 2장 이론적 배경 6
제 1절 건강노화 6
1. 건강노화의 정의 6
2. 건강노화의 다차원적 개념 6
3. 건강노화 개념의 발전과 적용 8
4. 건강노화의 중요성 9
5. 건강노화의 영역 10
제 2절 건강노화와 경제활동 12
제 3절 경제활동과 거주지역 13
제 3장 연구방법 14
제 1절 분석자료 및 연구대상 14
1. 분석자료 14
2. 연구대상 15
제 2절 변수의 정의 및 측정 17
1. 인구사회학적 특성 17
2. 건강노화지수 18
3. 경제활동 20
4. 거주지역 20
제 3절 연구모형 및 분석방법 21
1. 분석절차 21
2. 연구모형 및 분석방법 21
3. 모형평가 30
제 4장 연구결과 32
제 1절 연구대상자 및 주요변인의 일반적 특성 32
1. 인구사회학적 특성 32
2. 연령별 인구사회학적 특성 34
3. 거주지역에 따른 경제활동 여부 36
4. 연구대상자의 연령집단별 및 거주지역별 경제활동 여부 37
5. 건강노화지수의 분포 39
6. 연령별 건강노화지수의 분포(5차, 6차, 7차) 39
7. 연령별 건강노화 영역별 평균(5차, 6차, 7차) 43
8. 인구사회학적 특성에 따른 건강노화 46
9. 연령군별 경제활동에 따른 건강노화 48
제 2절 연구모형의 분석결과 50
1. 자기회귀교차지연모형 분석결과 50
2. 다집단 분석결과 56
제 5장 논의 및 결론 61
제 1절 논의 61
1. 인구사회학적 특성 61
2. 연령집단 및 거주지역과 경제활동 62
3. 건강노화지수 62
4. 경제활동과 건강노화지수 63
5. 자기회귀교차지연 모형 64
6. 연령별 다집단 분석 65
7. 연구의 제한점 66
제 2절 결론 및 제언 67
참고문헌 70
부록 7