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CT영상을 이용한 외상성 흉요추 골절의 자동화된 진단 및 분류를 위한 심층신경망
No full textDoctorⅠ. Introduction 1
Ⅱ. Material and methods · 3
A. Study subjects 3
B. Preparation and Annotation of Ground Truth Labels · 4
C. Deep Learning-Based Algorithm 5
D. Statistical analyses 6
Ⅲ. Results 7
A. Data Characteristics · 7
B. Performance of the models 8
1. Per-vertebra level 8
2. Per-segment level 9
Ⅳ. Discussion 12
Ⅴ. Conclusions · 16
VI. References 1
Adherence to Cancer Screening Services Among Koreans with Diabetes and Hypertension Using the Korea National Health and Nutrition Examination Survey
No full textDoctorChapter 1. Introduction . 1
A. Background . 1
B. Objective 7
Chapter 2: Methodology. 8
A. Study design and data sources 8
B. Study population 9
C. Exposures 10
D. Independent variables 12
E. Dependent Variables 13
F. Statistical Analysis 14
Chapter 3: Results 16
A. Baseline characteristics 16
B. Cancer Screening Rates According to Diabetes and Hypertension. 18
C. Cancer screening rate according to age, education, and income levels with respect to diabetes and hypertension. 20
D. National and individual Cancer screening rate according to diabetes and hypertension. 25
Chapter 4: Discussion 26
A. Limitations 32
Chapter 5: Conclusion 33
Bibliographic References 34
Appendix 1: Cancer screening protocol issued by the National Cancer Screening Program in Korea. 4
대사 이상 관련 지방성 간질환의 진행에 기여하는 신종 변이 식별을 위한 유전체 및 전사체 통합 분석
No full textDoctorI. INTRODUCTION 1
II. MATERIALS AND METHODS 3
A. Patients and tissue specimens 3
B. Transcriptomic profiling 3
C. Genomic variants profiling 4
D. Data integration and molecular subtype analyses 5
E. Cell type analysis 6
F. Statistical analyses 6
III. RESULTS 7
A. Description of overall study design 7
B. The transcriptomic spectrum of MASLD 11
C. Identification of pathogenic variants contributing to the progression of MASLD 26
D. IFI16 expression is attributed to the infiltrated macrophages during the progression of MASLD 45
E. IFI16 variant aggravates mitochondrial-related inflammatory responses 59
IV. DISCUSSION 72
V. CONCLUSION 77
VI. REFERENCES 78
국문 요약 8
Investigation of the LKB1–TSSK1B axis and GPR54 signaling in the Hippo-YAP pathway-mediated tumorigenesis
No full textDoctorIntroduction 1
PART I. The LKB1-TSSK1B axis inhibits tumor growth through YAP phosphorylation 5
I. Introduction 6
II. Aims of study 9
III. Materials and methods 12
A. Cell culture and transfection 12
B. Virus production and infection 12
C. Western blotting and immuoprecipitation (IP) 12
D. Subcellular fractionation 13
E. Luciferase assay 13
F. Recombinant protein purification 13
G. In vitro kinase assay 14
H. Immunofluorescence staining 14
I. Mutagenesis 15
J. Generation of knockout cell lines 15
K. TA cloning 16
L. shRNA transduction 16
M. Sulforhodamine B (SRB) assy 16
N. Soft agar assay 17
O. Xenograft 17
P. Antibodies and Reagents 17
Q. RNA extraction and quantitative real-time polymerase chain reactions (qRT-PCR) 18
R. Statistical analysis 19
IV. Results 20
1. TSSK1B triggers phosphorylation of YAP, leading to the suppression of its transcriptional activity 20
2. Endogenous TSSK1B modulates YAP phosphorylation and target genes activation 23
3. Endogenous TSSK1B modulates YAP phosphorylation through the Hippo pathway stimulation 26
4. TSSK1B induces YAP phosphorylation through upregulation of LATS1/2 activity via MAP4K4/6/7- and MST1/2-dependent mechanism 29
5. LKB1, the upstream kinase of TSSK1B, induces TSSK1B phosphorylation and mediate subcellular localization 32
6. LKB1-mediated activation of TSSK1B induces phosphorylation of YAP and modifies its subcellular localization 36
7. TSSK1B phosphorylates YAP independent of AMPKα1/2 39
8. TSSK1B directly phosphorylates YAP at Ser94 to regulate its activity 42
9. TSSK1B suppresses tumorigenic potential of YAP without LATS1/2 dependency 45
V. Discussion 50
PART II. GPR54 signaling induces YAP activation through a positive feedback loop in breast cancer 53
I. Introduction 54
II. Aims of study 57
III. Materials and methods 61
A. Cell culture and transfection 61
B. Antibodies and Reagents 61
C. Virus production and transduction 62
D. Western blot and immuoprecipitation (IP) 62
E. In vitro kinase assay 62
F. Luciferase assay 63
G. RNA isolation and quantitative real-time polymerase chain reactions (qRT-PCR) 63
H. Immunofluorescence staining 64
I. Site-directed mutagenesis 64
J. Generation of knockout cell lines using CRISPR/Cas9 system 65
K. TA cloning 65
L. siRNA transfection 66
M. shRNA transduction 66
N. Sulforhodamine B (SRB) assay 66
O. Soft agar assay 67
P. Clonogenic assay 67
Q. Chromatin immunoprecipitation (ChIP-IP) 67
R. Invasion assay 68
S. Migration assay 69
T. Statistical analysis 69
IV. Results 70
1. GPR54 signaling enhances YAP-TEAD transcriptional activity 70
2. Wild-type of GPR54 signaling facilitating YAP activation 73
3. GPR54 signaling enhances YAP activity through Gαq/11 and RhoA activation 76
4. GPR54 signaling inhibits LATS1/2 kinase activity 81
5. GPR54 signaling has a positive feedback loop with the Hippo-YAP pathway 84
6. GPR54 signaling facilitates cancer progression through the Hippo-YAP pathway 88
7. Verteporfin and p234 antagonist regulate YAP activity via GPR54 signaling 91
8. Deletion of YAP attenuates oncogenic potentials by GPR54 signaling 94
V. Discussion 97
Conclusion 100
Reference 102
국문요약 11
건강보험가입자와 의료급여자의 일개 지역응급의료센터 체류시간 비교
No full textMasterI. 서 론 1
1) 연구의 필요성 - 1
2) 연 구 목 적 - 5
3) 용 어 정 의 - 6
II. 문 헌 고 찰 9
1) 건강보장제도 - 9
2) 건 강 보 험 - 12
3) 의 료 급 여 - 14
4) 응급의료센터 체류시간과 과밀화 - 17
5) 일차보건의료 - 20
III. 연 구 방 법 22
1) 연 구 설 계 - 22
2) 연구대상자 및 표본크기 - 23
3) 자료수집과정 - 24
4) 자료분석방법 - 24
IV. 결 과 25
1) 대상자의 일반적 특성 - 25
2) 대상자의 내원 특성 - 26
3) 대상자의 진료 특성 - 27
4) 대상자의 일반적 특성에 따른 체류시간 - 28
5) 대상자 내원 특성에 따른 체류시간 - 29
6) 대상자 진료 특성에 따른 체류시간 - 30
7) 체류시간 결정 요인 - 31
8) 보험 유형에 따른 대상자 특성 차이 - 33
V. 논 의 37
VI. 결 론 44
참 고 문 헌 4
임상간호사의 노인간호수행에 영향을 미치는 요인
No full textMasterⅠ. 서론 1
A. 연구의 필요성 1
B. 연구목적 4
C. 용어정의 5
Ⅱ. 문헌고찰 7
A. 임상간호사의 노인간호수행 7
B. 노인간호수행의 영향요인 9
Ⅲ. 연구방법 13
A. 연구설계 13
B. 연구대상 13
C. 연구도구 15
D. 자료수집 방법 17
E. 윤리적 고려 17
F. 자료분석 방법 18
Ⅳ. 연구결과 19
A. 연구대상자의 일반적 특성 및 직무 관련 특성 19
B. 연구대상자의 연령주의, 공감역량, 노인간호수행 정도 21
C. 연구대상자의 일반적 특성 및 직무관련 특성에 따른 노인간호수행의 차이 22
D. 연구대상자의 노인간호수행과 연령, 연령주의, 공감역량 간의 상관관계 24
E. 연구대상자의 노인간호수행에 영향을 미치는 요인 26
Ⅴ. 논의 28
Ⅵ. 결론 및 제언 34
A. 결론 34
B. 제언 35
참고문헌 36
부록 45
ABSTRACT 5
대장 마크로파지에서의 CD206이 대장암에 미치는 영향
No full textMasterⅠ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 2
1. Bioinformatics analysis 2
2. Mice & Colitis and CAC model 2
3. Macrophage subset analysis 3
4. Foxp3+ Treg subset analysis 4
5. Culture for Bone marrow-derived macrophages 4
6. RNA extraction & Real-time PCR 5
7. Sorting for colon F4/80+ cells 6
8. Histology 6
9. Bacterial infection 7
10. Statistics 7
Ⅲ. RESULTS 11
A. Bioinformatics analysis of CD206 in colon adenocarcinoma. 11
B. Deficiency of CD206 increased inflammatory characteristic of macrophages in steady state. 14
C. Deficiency of CD206 can affect colon Treg population in steady state. 17
D. Deficiency of CD206 significantly affect colon inflammation in DSS state. 19
E. Deficiency of CD206 increased colonic tumor formation in AOM/DSS state. 23
F. Deficiency of CD206 in tissue induced inflammatory cytokines and tumor-related factors after AOM/DSS treatment. 26
G. Deficiency of CD206 in macrophage induced inflammatory cytokines and chemokine after AOM/DSS treatment. 28
H. Deficiency of CD206 showed higher defense against pathogenic bacterial infection. 30
Ⅳ. DISCUSSION 32
Ⅴ. CONCLUSION 35
REFERENCES 38
KOREAN ABSTRACT 4
Oncologic and Operative Outcomes of Robotic Staging Surgery Using Low Pelvic Port Placement in High-Risk Endometrial Cancer
No full textUpper para-aortic lymph node dissection (PALND) is one of the most challenging gynecologic robotic procedures. This study aimed to evaluate the oncologic and operative outcomes of robotic staging surgery, including upper PALND, using low pelvic port placement (LP3) in 22 patients with high-risk endometrial cancer. High-risk was defined as patients who showed deep myometrial invasion with grade III, cervical involvement, or high-risk histology. The mean patient age and body mass index were 58 years and 24 kg/m2. The mean operative time was 263 min. The mean number of total LNs and upper PALNs obtained was 31 and 10. Two patients received lymphangiography to reduce the amount of drained lymphatic fluid after surgery. The recurrence rate was 13.6% (3/22). There were two LN recurrences and one at the peritoneum in the intra-abdominal cavity. Robotic staging surgery using LP3 was feasible for performing PALND as well as procedures in the pelvic cavity simultaneously. It provides important techniques for performing optimal surgical procedures when surgeons decide to perform comprehensive PALND in instances of isolated recurrence or unexpected LN enlargement as well as high-risk endometrial cancer. Consequently, surgeons can achieve surgical consistency and reproducibility for PALND, leading to improved operative and survival outcomes in high-risk endometrial cancer
Plasma high‐density lipoprotein levels is associated with preserved hippocampal volume in Alzheimer`s disease individuals without vascular co‐pathology
No full textBACKGROUND: Dysregulation of cholesterol metabolism contributes to the increase of cerebral vascular diseases, favoring the development of dementia. In this sense, high levels of high-density lipoprotein (HDL) are considered protective against the outcomes associated with cardiovascular diseases. However, little is known about the effect of plasma HDL levels to alterations of cerebral volume in non-vascular AD and vascular AD individuals. Here we evaluated the association of plasma HDL levels with alterations in brain volume and with plasma neurofilament light chain (NfL) levels. METHOD: We analyzed 135 AD individuals from the Biobank Innovations for Chronic Cerebrovascular Disease with Alzheimer's Disease Study [BICWALZS] cohort. Based on Fazekas scale for brain white matter lesions, we stratified AD individuals into two groups: non-vascular AD [Fazekas score = 1 and amyloid-β (Aβ) positive/n = 76], and vascular AD [Fazekas score = 2, 3 and Aβ positive/n = 59] (Figure 1A). Linear regression models accounting for vascular co-pathology APOEε4 carriership, age and sex were used to test associations of plasma HDL with the variables of interest. RESULT: We observed a significant interaction between plasma HDL levels and presence of vascular pathology on total brain white matter (b = 0.375, p = 0.019, Fig. 1B; Table 2). Analyzing the hippocampal atrophy through the global Schelten`s scale, we observed that high plasma HDL levels was negatively associated with hippocampal atrophy only in non-vascular AD individuals (b = -0.405, p < 0.001, Fig. 1C). However, no effect of plasma HDL levels on total gray matter and hippocampal volume was observed (Table 1). Non-vascular AD individuals with high hippocampal atrophy scores showed reduced plasma HDL levels compared to non-vascular AD individuals with low hippocampal atrophy scores (p < 0.01, Fig. 1D). Furthermore, no associations of plasma HDL with NfL levels were detected, regardless of the brain vascular status (Fig. 1E). CONCLUSION: We showed that high plasma HDL levels is associated with less white matter pathology and reduced hippocampal atrophy scores, without changing plasma NfL levels. Together our findings indicate that high HDL levels seem to be associated with preserved brain volume only in AD individuals with no vascular co-pathology
Correlation between individual thigh muscle volume and grip strength in relation to sarcopenia with automated muscle segmentation
No full textIntroduction: Grip strength serves as a significant marker for diagnosing and assessing sarcopenia, particularly in elderly populations. The study aims to explore the relationship between individual thigh muscle volumes and grip strength, leveraging advanced AI-based UNETR segmentation techniques for accurate muscle volume assessment.
Methods: The study included 49 participants from a cohort of 478 patients diagnosed with hip fractures at Gyeongsang National University Hospital. We recorded Grip strength and height and utilized UNETR-based segmentation techniques on CT scans, to calculate individual thigh muscle volumes. Point-biserial correlation was employed to explore the relationship between sarcopenia and thigh muscle volumes. The research also included a quantile analysis of grip strength.
Results: Our findings revealed a strong statistical significance in specific thigh muscles like Rectus femoris, Vastus lateralis, and Vastus intermedius, particularly in males, in relation to sarcopenia. The male cohort displayed a trend where higher thigh muscle volumes correlated with better grip strengths. Meanwhile no such relationship was found within the female group.
Conclusion: The findings indicate that stronger grip strength correlates with larger thigh muscles in males but not in females, with specific muscles like the Rectus femoris and Vastus lateralis linked to sarcopenia in men only. The study's small sample size calls for further research with more diverse and gender-balanced groups to verify these results