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Establishment of TH-EGFP human embryonic stem cell line for specific labeling of dopaminergic neurons
Get PDFDopaminergic (DA) neurons play critical roles in various neurological processes and disorders, particularly Parkinson's disease. To enable precise visualization and tracking of DA neurons, we generated TH-EGFP, a tyrosine hydroxylase (TH)-driven enhanced green fluorescent protein (EGFP)-expressing knock-in cell line, by employing CRISPR/Cas9 technology. We introduced EGFP into the targeted genomic region of human embryonic stem cells (hESCs) and successfully established a TH-EGFP hESC line. Differentiation of TH-EGFP hESCs into human midbrain organoids confirmed the accurate integration of EGFP into TH-positive cells. The TH-EGFP hESC line serves as a valuable reporter for studying the development, maturation, and function of DA neurons
Deep learning-based surgical phase recognition in laparoscopic cholecystectomy
Get PDFBackgrounds/Aims: Artificial intelligence (AI) technology has been used to assess surgery quality, educate, and evaluate surgical performance using video recordings in the minimally invasive surgery era. Much attention has been paid to automating surgical work-f low analysis from surgical videos for an effective evaluation to achieve the assessment and evaluation. This study aimed to design a deep learning model to automatically identify surgical phases using laparoscopic cholecystectomy videos and automatically assess the accuracy of recognizing surgical phases. Methods: One hundred and twenty cholecystectomy videos from a public dataset (Cholec80) and 40 laparoscopic cholecystectomy videos recorded between July 2022 and December 2022 at a single institution were collected. These datasets were split into training and testing datasets for the AI model at a 2:1 ratio. Test scenarios were constructed according to structural characteristics of the trained model. No pre-or post-processing of input data or inference output was performed to accurately analyze the effect of the label on model training. Results: A total of 98,234 frames were extracted from 40 cases as test data. The overall accuracy of the model was 91.2%. The most accurate phase was Calot’s triangle dissection (F1 score: 0.9421), whereas the least accurate phase was clipping and cutting (F1 score: 0.7761). Conclusions: Our AI model identified phases of laparoscopic cholecystectomy with a high accuracy
콕사키바이러스 B3에 의해 유발된 바이러스성 심근염에서 parkin과 PINK1의 역할에 대한 연구
No full textDoctorI. INTRODUCTION 1
A. Myocarditis and dilated cardiomyopathy (DCM) 1
B. Causes and clinical presentations of myocarditis 1
C. Coxsackievirus B3 (CVB3) 2
D. Parkinson's disease 3
E. Parkin 3
F. PINK1 4
G. Parkin and PINK1 in the defense mechanism against pathogens 5
H. Aims 5
II. MATERIALS AND METHODS 6
A. Antibodies and Reagents 6
B. Animals 6
C. Virus 7
D. Murine viral myocarditis model 7
E. Primary cardiomyocyte culture and infection with CVB3 8
F. Immunostaining 9
G. Inflammation scoring 10
H. EBD staining 10
I. RNA isolation and quantitative real-time PCR (qPCR) 10
J. Western blot analysis 13
K. IFN-γ treatment 13
L. Transmission electron microscopy (TEM) 13
M. Echocardiogram 14
N. Public RNA-seq data and variants calling 14
O. Statistical analysis 15
III. RESULTS 16
Part A. Effects of parkin and PINK1 on the progression of viral myocarditis 16
Part B. Effects of parkin and PINK1 on antiviral immunity 22
Part C. Effects of parkin and PINK1 on mitochondrial vulnerability 42
Part D. Pathogenic variants of parkin and PINK1 in patients 46
IV. DISCUSSION 54
V. CONCLUSION 62
VI. REFERENCES 63
국문요약 7
Egg White Food Ladder: Evaluation of Cooking Effect on Hen's Egg White
No full textDoctorⅠ. Introduction 1
Ⅱ. Materials and Methods 4
A. Part Ⅰ. Crude extract preparation of various cooked hen's egg white and setup experimental protocol 4
1. Materials 4
2. Crude extract preparation procedure 4
3. SDS-PAGE 7
4. Study sera population 7
5. Setup of Raw Egg White Immunoblot protocol 8
a. Protocol 1 8
b. Protocol 2 9
c. Protocol 3 9
d. Protocol 4 9
6. Setup of various Egg White protein Immunoblot protocol 10
a. Protocol 1 10
b. Protocol 2 10
7. ELISA protocol set-up 11
B. Part Ⅱ. Evaluation of immunoglobulin IgE binding capacities in egg ladder 12
1. Study sera population 12
2. IgE-Immunoblot 12
3. Individual IgE ELISA 13
4. Inhibition ELISA 14
5. Statistical analysis 14
Ⅲ. Results 15
A. PART Ⅰ. Crude extract preparation of various cooked hen's egg white and experimental protocol set-up 15
1. SDS-PAGE protein profiles of crude raw, scramble, boiled, short-baked and long-baked HEW 15
2. Study patient sera of setup protocol 15
3. Setup of Raw Egg White Immunoblot protocol 16
a. Protocol 1 16
b. Protocol 2 16
c. Protocol 3 16
d. Protocol 4 17
4. Setup of Various Egg White proteins Immunoblot protocol 17
a. Protocol 1 17
b. Protocol 2 17
B. PART Ⅱ. Evaluation of immunoglobulin IgE-binding capacities in egg ladder 28
1. Clinical and immunological characteristics of patients 28
2. Specific IgE levels in the tested sera 28
3. IgE Immunoblot 29
4. IgE Individual ELISA 29
5. IgE Inhibition ELISA of raw EW extract 30
IV. Discussion 37
V. Conclusion 40
References 41
국문요약 4
노인 만성폐쇄성폐질환자의 자가간호 영향요인
No full textMaster1. 서 론 1
A. 연구의 필요성 1
B. 연구의 목적 5
C. 용어의 정의 6
2. 문헌고찰 8
A. 노인 만성폐쇄성폐질환자의 자가간호 8
B. 노인 만성폐쇄성폐질환자의 자가간호 영향요인 11
3. 연구방법 14
A. 연구설계 14
B. 연구대상 14
C. 연구도구 15
D. 자료수집 방법 18
E. 자료분석 방법 19
F. 윤리적 고려 20
4. 연구결과 21
A. 대상자의 일반적 특성 및 질병 관련 특성 21
B. 대상자의 자가간호, 증상경험, 자기효능감, 사회적지지 수준 24
C. 대상자의 일반적 특성 및 질병 관련 특성에 따른 자가간호 수준의 차이 26
D. 대상자의 자가간호, 증상경험, 자기효능감, 사회적지지 간의 상관관계 29
E. 대상자의 자가간호 영향요인 30
5. 논의 32
6. 결론 및 제언 37
참고문헌 38
부록 46
ABSTRACT 6
알코올 사용장애 환자를 위한 자기결정성 이론기반 회복 증진 프로그램 개발 및 효과
No full textDoctorⅠ. 서론 1
A. 연구의 필요성 1
B. 연구의 목적 5
C. 연구의 가설 5
D. 용어의 정의 6
Ⅱ. 문헌고찰 8
A. 알코올 사용장애 환자 8
B. 알코올 사용장애 환자의 회복 증진을 위한 프로그램 13
C. 자기결정성 이론 16
Ⅲ. 개념적 기틀 20
Ⅳ. 연구방법 23
A. 자기결정성 이론 기반 회복 증진 프로그램 개발 23
B. 자기결정성 이론 기반 회복 증진 프로그램 효과 검증 37
Ⅴ. 연구결과 47
A. 실험군과 대조군의 동질성 검정 47
B. 연구의 가설 검정 50
Ⅵ. 논의 60
A. 자기결정성 이론 기반 회복 증진 프로그램 개발 60
B. 자기결정성 이론 기반 회복 증진 프로그램의 효과 63
C. 연구의 의의 72
Ⅶ. 결론 및 제언 73
A. 결론 73
B. 제언 74
참고문헌 75
부록 94
ABSTRACT 12
상급종합병원 중환자실 간호사의 신체보호대 간호수행 영향요인
No full textMasterⅠ. 서론 1
A. 연구의 필요성 1
B. 연구목적 5
C. 용어의 정의 6
Ⅱ. 문헌고찰 8
A. 중환자실 간호사의 신체보호대 간호수행 8
B. 중환자실 간호사의 신체보호대 간호수행 영향요인 10
Ⅲ. 연구방법 13
A. 연구설계 13
B. 연구대상 13
C. 연구도구 14
D. 자료수집방법 16
E. 윤리적 고려 17
F. 자료분석방법 18
Ⅳ. 연구결과 19
A. 대상자의 일반적 특성 19
B. 대상자의 인간중심간호, 신체보호대 적용에 대한 인식, 태도, 신체보호대 간호수행 21
C. 대상자의 일반적 특성에 따른 신체보호대 간호수행의 차이 23
D. 대상자의 인간중심간호, 신체보호대 적용에 대한 인식, 태도, 신체보호대 간호수행 간의 상관관계 25
E. 대상자의 신체보호대 간호수행의 영향요인 27
Ⅴ. 논의 29
Ⅵ. 결론 및 제언 34
참고문헌 35
부 록 43
ABSTRACT 5
Study on identification of RIPK1 inhibitor for therapeutic potential : Identification of novel inhibitor of receptor-interacting protein kinase 1 (RIPK1)
No full textMasterI. INTRODUCTION 1
II. MATERIALS AND METHODS 4
A. Antibodies and chemical reagents 4
B. Cell lines and culture conditions 5
C. Immunoblot analysis and immunoprecipitation 5
D. Plasmid construction and transfection 6
E. Cytotoxicity assays 6
F. Immunofluorescence staining 6
G. Primary culture and activation of BMDMs 6
H. Flow cytometry 7
I. Quantitative RT-PCR 7
J. Histology analysis 7
K. Serum biochemistry 8
L. LPS-induced SIRS model 8
M. Structural similarity calculation 8
N. Molecular docking and dynamics simulation study 8
O. Statistical analysis 9
III. RESULTS 10
A. Identification of phensuximide as a potential RIPK 1 inhibitor. 10
B. Phensuximide shows potent inhibitory effect on TNF-induced necroptotic cell death. 15
C. Inhibition of RIPK1 kinase activity by phensuximide treatment. 23
D. Phensuximide does not involved in receptor-mediated complex I signal. 33
E. Inhibition of RIPK1 kinase activity-dependent cell death and inflammatory response by phensuximide in macrophages. 38
F. Phensuximide is a potential therapeutic candidate for necroptosis related diseases. 46
IV. DISCUSSION 52
V. REFERENCES 54
국문요약 5
사업장 보건관리자의 심뇌혈관질환 예방관리 업무역량과 관련된 요인
No full textMaster제 1 장 서론 1
제 1 절 연구의 필요성 1
제 2 절 연구의 목적 5
제 3 절 용어의 정의 6
제 2 장 연구방법 9
제 1 절 연구설계 9
제 2 절 연구대상자 및 표본크기 10
제 3 절 자료수집과정 10
제 4 절 측정도구 11
제 5 절 분석방법 19
제 3 장 결과 20
제 4 장 고찰 33
제 5 장 결론 39
참고문헌 4
Hippocampal degeneration is associated with GFAP levels in individuals with vascular dementia independently of amyloidosis
No full textBACKGROUND: Vascular cognitive impairment/dementia (VD) is the second most prevalent cause of dementia following Alzheimer's disease (AD). VD is characterized by the progression of white matter hyperintensity burden (WMH) and associated neurodegeneration. GFAP, a biomarker for reactive astrogliosis, is associated with Aβ pathology and mediates tau-pathology in preclinical AD. However, the association of GFAP levels with the markers associated with VD is poorly understood. METHOD: We assessed 796 participants from a research cohort (TRIAD: 355) and a memory clinic cohort (BICWALZ: 441) that were divided into four groups according to their Aβ (Aβ+/Aβ-) and WMH status (WMH+/WMH-). WMH values were corrected by intracranial volume, and cutoffs were determined based on the highest WMH value within 50% of the individuals with the lowest WMH rate in the CU Aβ- group. Biomarker mean level differences between groups were estimated via Ancova Tukey's test, and linear regressions accounting for age, sex, and cognitive status were used to estimate the association. RESULT: For TRIAD, WMH+/Aβ+ individuals exhibit higher levels of plasma GFAP when compared to all groups except WMH-/Aβ+, with no differences in NfL levels and hippocampal volume between groups (Fig. 1A). In BICWALZ, only hippocampal volume differs between groups with lower levels observed in both WMH-/Aβ- and WMH-/Aβ+ (Fig. 1B''). The association of GFAP and NfL was observed in all groups (Fig. 2A, 2B'). On the other hand, hippocampal degeneration was associated with higher GFAP levels in individuals with abnormal WMH and absence of Aβ burden in both cohorts (Fig. 3A: TRIAD: β = -0.3036; p = 0.0195; Fig. 3B': BICWALZ: β = -0.1791; p = 0.0125). CONCLUSION: Our findings suggest that astrocyte reactivity, as indicated by plasma GFAP levels, plays a significant role in the hippocampal atrophy observed in patients with vascular disease. These results suggest that therapies targeting astrocyte reactivity could potentially alleviate progressive cognitive deficits commonly found in patients with chronic vasculopathy