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Functional structure of testate amoeba community at Trstenik peatbog
No full textCretovi predstavljaju prijelazna podruĉja izmeĊu vodenih i kopnenih ekosustava s bogatom bioraznolikońću. Na cretu Trstenik, ombrotrofnom cretu iz ledenog doba smjeńtenog iznad Klane u Gorskom kotaru, utvrĊene su sukcesijske promjene zbog ńirenja trave modre beskoljenke koje su dovele do isuńivanja creta. Okućene amebe su jednostaniĉni heterotrofni eukarioti ĉija je stanica zańtićena vanjskom kućicom. Zbog kozmopolitske rasprostranjenosti te kratkog ņivotnog vijeka, okućene amebe posebno su osjetljive na promjene u okolińu, a na cretovima sluņe kao bioindikatori promjene okolińnih uvjeta. Ciljevi ovog diplomskog rada su: i) odrediti funkcionalnu strukturu okućenih ameba; ii) usporediti funkcionalnu strukturu cretnog stanińta s rubnim stanińtima i sukcesijskim stanińtem; iii) povezati funkcionalnu strukturu s mikroklimatskim uvjetima na cretu Trstenik. Funkcionalne znaĉajke na temelju kojih je odreĊena funkcionalna struktura okućenih ameba su: tip pseudopodija, sastav kućice, spljońtenost kućice, oblik kućice, veliĉina otvora, poloņaj otvora, oblik otvora te miksotrofija. Rezultati ovog diplomskog rada potvrdili su sukcesijske promjene kojima je zahvaćen cret Trstenik te pokazali da struktura funkcionalnih znaĉajki okućenih ameba odraņava prilagodbe cretnom stanińtu za većinu funkcionalnih znaĉajki. Funkcionalna struktura okućenih ameba cretnog stanińta, razlikuje se od funkcionalne strukture okućenih ameba rubnog stanińta i sukcesijskog stanińta, a ista odraņava mikroklimatske uvjete na razliĉitim stanińtima creta Trstenik, posebice uvjete vlaņnosti supstrata.Peatbogs, which serve as transition zones between aquatic and terrestrial ecosystems, are characterized by great biodiversity. In Trstenik petabog, an ombrotrophic peatbog from the Ice Age above Klana in Gorski Kotar, successional changes were observed due to the spread of purple moor-grass, which led to the drying out of the peatbog. Testate amoebae, unicellular eukaryotes with a protective outer shell, are particularly sensitive to environmental changes and serve as bioindicators of changing environmental conditions in peatbogs. This study aims to: i) determine the functional structure of testate amoebae; ii) compare the functional structure in peatbog habitat with edge and successional habitat; iii) establish connections between functional structure with microclimatic conditions in the Trstenik peatbog. Functional traits, including pseudopod type, test composition, test flatness, test shape, aperture size, aperture position, aperture shape and mixotrophy, were analyzed. The results confirm changes in succession and show that the functional structure of testate amoebae reflects adaptations to habitat, with variations observed between typical peatbog habitat, edge and successional habitat, particularly in response to substrate moisture conditions
Effect of iron nanoparticles functionalization on Er(III) ion sorption from aqueous solutions
No full textErbijevi spojevi najviše se koriste u izradi optičkih vodiča, lasera i u nuklearnim industrijama te stoga dospijevaju u okoliš. Zbog ograničenih resursa, učinkovito odvajanje erbija predmet je brojnih istraživanja. U ovom radu provedeni su u vodenom mediju eksperimenti sorpcije iona Er3+ pri različitim početnim koncentracijama na neutralnim nanočesticama željeza (nZVI) i nanočesticama željeza modificiranim s dipikolinskom kiselinom (nZVI/PDCA). Nefunkcionalizirane neutralne nanočestice željeza nZVI pokazale su iznimnu efikasnost sorpcije iona erbija (97-99 %) dok su modificirane čestice nZVI/PDCA pri istim dodanim koncentracijama iona erbija u vodenoj otopini za 16 % manje efikasne. Morfološke značajke nefunkcionaliziranih i modificiranih nanočestica prije i nakon sorpcije iona Er3+ dobivene metodom SEM ukazuju na sferične lančaste strukture nemodificiranih nanočestica te sitnije eliptične nakupine modificiranih nanočestica. Utvrđene razlike u morfologiji kod obje vrste čestica pridonose razumijevanju mogućih mehanizama sorpcije.Erbium compounds are mostly used in the production of optical guides, lasers and in the nuclear industry, and therefore end up in the environment. Due to limited resources, the efficient separation of erbium is the subject of numerous investigations. In this work, Er3+ ion sorption experiments in an aqueous medium at different initial concentrations on neutral iron nanoparticles (nZVI) and particles modified with dipicoline acid (nZVI/PDCA) were carried out. Non-functionalized nZVI nanoparticles showed exceptional erbium ion sorption efficiency (97-99 %), while modified nZVI/PDCA nanoparticles at the same added concentrations of erbium ions in water are 16% less efficient compared to non-functionalized nZVI particles. Morphological features of neutral iron nanoparticles and nanoparticles modified with dipicoline acid before and after Er3+ ion sorption obtained by the SEM method indicate spherical chain structures of bare particles and smaller elliptical clusters of modified particles. The determined differences in the morphology of both types of nanoparticles contribute to the understanding of possible sorption mechanisms
The origin and composition of the Mura River sediments
No full textU ovome radu analiziran je granulometrijski i modalni sastav te ukupna koncentracija metala u recentnim riječnim sedimentima. Uzorci su prikupljeni s 11 različitih lokacija duž dijela toka rijeke Mure koji prolazi Republikom Hrvatskom. Rezultati granulometrijskih analiza pokazali su da se većina sedimenata može determinirati kao srednje do vrlo loše sortirani prahoviti pijesak. Analizom modalnog sastava utvrđeno da je kvarc najzastupljeniji sastojak sedimenata. U zajednici teških minerala najzastupljeniji su granati, amfiboli i minerali iz skupine epidota. Utvrđena mineralna zajednica karakteristična je za sedimente porijeklom iz istočnih Alpa. Analizom ukupnih koncentracija 18 elemenata u prikupljenim sedimentima utvrđeno je da su koncentracije metala poput Cr, Al, Zn, Co, Ni, Sn povišene u odnosu na literaturne podatke. Smatra se da su glavni razlozi tome uglavnom antropogene prirode, a među njima ističu se nekadašnji rudnici ugljena na tome prostoru, velika farmaceutska industrija i poljoprivredne aktivnosti.In this study, the grain size and mineral composition as well as the total element concentration in recent river sediments were analysed. Samples were taken from 11 different locations along the part of the Mura River that flows through the Republic of Croatia. The results of the grain size analyses showed that most of the sediments can be determined as medium to very poorly sorted silty sand. The analysis of the mineral composition showed that quartz is the most abundant component of the sediments. The most abundant heavy minerals are garnets, amphiboles and minerals from the epidote group. The identified mineral assemblage is characteristic for sediments from the Eastern Alps. The analysis of the total concentrations of 18 elements in the collected sediments showed that the concentrations of metals such as Cr, Al, Zn, Co, Ni, Sn are elevated compared to literature data. It is assumed that the main reasons for this are of anthropogenic nature, such as former coal mines in the region, large pharmaceutical industry and agricultural activities
Nature based tourism in the slunjsko-plitvička area
No full textOvaj rad istražuje turizam temeljen na prirodi u slunjsko-plitvičkom kraju, s naglaskom na povezanost Nacionalnog parka Plitvička jezera s ostalim atrakcijama u blizini. Cilj istraživanja bio je analizirati motivaciju, aktivnosti, obilježja putovanja i zadovoljstvo posjetitelja, kao i ispitati turističku ponudu i infrastrukturu ovog područja. Podaci su prikupljeni putem anketnog istraživanja posjetitelja Nacionalnog parka i Rastoka. Rezultati istraživanja pokazuju visoko zadovoljstvo posjetitelja s prirodnim ljepotama i kulturnom baštinom, no također ukazuju na nedostatak koordinacije između različitih turističkih lokaliteta, što može rezultirati fragmentiranim iskustvom za posjetitelje. Iako postoje jasne prednosti u prirodnim resursima, infrastruktura nije dovoljno razvijena i usklađena s potrebama svih dionika, a povećan turistički pritisak stvara izazove u održavanju i očuvanju tih resursa. Istraživanje naglašava potrebu za integracijom svih ključnih atrakcija u jedinstvenu ponudu, koja bi omogućila lakšu povezanost između lokaliteta, te za održivim razvojem turizma koji poštuje prirodne i kulturne vrijednosti. Daljnji razvoj trebao bi uključivati bolju suradnju između lokalne zajednice, pružatelja usluga i turističkih dionika, čime bi se stvorila dugoročna održivost ovog područja.This thesis explores nature-based tourism in slunjsko-plitvička area, with a focus on the connection between Plitvice Lakes National Park and other nearby attractions. The aim of the research was to analyse visitor motivation, activities, travel characteristics, and satisfaction, as well as to examine the tourism offerings and infrastructure of the region. Data was collected through a survey conducted with visitors of Plitvice Lakes National Park and Rastoke. The findings show high visitor satisfaction with the natural beauty and cultural heritage, but also point to a lack of coordination between various tourist sites, which can result in a fragmented experience for visitors. While there are clear advantages in natural resources, the infrastructure is not sufficiently developed and aligned with the needs of all participants, and increased tourist pressure creates challenges in maintaining and preserving these resources. The research highlights the need to integrate all key attractions into a unified offering, which would improve connectivity between sites, and for sustainable tourism development that respects natural and cultural values. Further development should include better collaboration between the local community, service providers, and tourists, creating long-term sustainability for the area
Quantum memory in warm rubidium atomic vapor
No full textRazvoj kvantnih tehnologija−osobito kvantne komunikacije, računala i senzora ukazao je na potrebu za kontroliranim pohranjivanjem kvantne informacije radi distribucije isprepletenih stanja i sinkronizacije kvantnih mreža. To je potaknulo razvoj optičkih kvantnih memorija koje omogućuju pohranu kvantnih stanja svjetlosti i njihovo otpuštanje na zahtjev. Cilj ovog diplomskog rada je razvoj, karakterizacija i optimizacija ključnih komponenti za realizaciju kvantne memorije u toplim parama atoma rubidija, temeljene na efektu elektromagnetski inducirane transparencije (EIT). Rad započinje osvrtom u kojem se pomoću optičkih Blochovih jednadžbi opisuje kako pojava elektromagnetski inducirane transparencije omogućuje pohranjivanje informacije u atomski medij te se identificiraju parametri ključni za rad i
optimizaciju performansi kvantne memorije. U prvom dijelu eksperimentalnog rada, korištenjem klasične svjetlosti određeni su optimalni uvjeti za rad optičke memorije s obzirom na vrijeme i učinkovitost pohrane svjetlosti. Utvrđeno je da se najviša efikasnost pohrane fotona postiže u nerezonantnoj EIT shemi, koja je stoga i detaljno istražena. U završnom dijelu diplomskog rada osmišljena je i dizajnirana nadogradnja postojećeg eksperimentalnog postava s ciljem prelaska na kvantne memorije, a testirane su i osnovne komponente sustava nužne za rad s pojedinačnim fotonima.The development of quantum technologies, particularly quantum communication, computing, and sensing has highlighted the need for controlled storage of quantum information for the distribution of entangled states and synchronization of quantum networks. This has driven the development of optical quantum memories, which enable the storage of quantum states of light and their on-demand retrieval. The aim of this thesis is the development, characterization, and optimization of key components for the implementation of a quantum memory in warm rubidium vapor, based on the effect of electromagnetically induced transparency (EIT). The work begins with a theoretical overview using the optical Bloch equations to describe how the phenomenon of electromagnetically induced transparency enables the storage of information in an atomic medium, and it identifies the parameters critical for the operation and optimization of the quantum memory. In the first part of the experimental thesis, optimal conditions for the operation of the optical memory were determined with respect to the storage time and efficiency using classical light. It was found that the highest photon storage efficiency is achieved in an off-resonant EIT scheme, which was therefore investigated in detail. In the final part of the thesis, an upgrade to the existing experimental setup was designed and implemented with the goal of transitioning to quantum memory operation. Basic components necessary for working with single photons were also tested
Application of gold and selenium nanoparticles for improving L-dopa therapy for Parkinson's disease in Wistar rat
No full textParkinsonova bolest druga je najčešća neurodegenerativna bolest koju karakterizira propadanje dopaminergičnih neurona u supstanciji nigri i, posljedično, manjak dopamina. Terapijski pristupi uglavnom su usmjereni na motoričke simptome i nažalost gube svoju učinkovitost kako neurodegeneracija i simptomi napreduju. Levodopa je trenutačni zlatni standard za liječenje Parkinsonove bolesti, no dugoročna primjena rezultira različitim nuspojavama. Liječenje neurodegenerativnih poremećaja još uvijek predstavlja veliki izazov zbog postojanja krvno-moždane barijere koja otežava efikasnu isporuku lijeka u mozak. Nanotehnologija nudi mogućnosti za poboljšanje terapijskih pristupa zbog sposobnosti nanomaterijala da budu nosači lijekova, da prolaze kroz krvno-moždanu barijeru, poboljšaju farmakološka svojstva i sigurnost lijekova te isporuče lijekove na ciljano mjesto. Nanočestice selena stabilizirane polivinilpirolidonom i nanočestice zlata stabilizirane peptidoglikanskim monomerom ispitane su kao nosači za isporuku levodope s ciljem postizanja neuroprotektivnog učinka. Određivana je njihova sposobnost smanjenja oksidativnog stresa uzrokovanog levodopom u in vivo modelu Parkinsonove bolesti. Štakori oba spola su podijeljeni u 7 skupina i tretirani testnim tvarima tijekom 28 dana. Raspodjela po skupinama bila je nasumična. Oksidativni stres istražen je mjerenjem reaktivnih kisikovih vrsta i koncentracije glutationa u mozgu, bubregu, jetri i serumu tretiranih i netretiranih štakora. Rezultati upućuju na neuroprotektivno djelovanje nanočestica selena. Nanočestice zlata ne pružaju zaParkinson’s disease is the second most common neurodegenerative disease characterized by deteriorating of dopaminergic neurons in the substance of nigra and, consequently, lack of dopamine. Present therapies are focused on reduction of motoric symptoms and sadly lose their efficiency as the neurodegeneration and symtpoms progress. Levodopa is currently a gold standard for curing Parkinson’s disease, but long-term application results in various side effects. Curing neurodegenerative disorders is still a major challenge because of existing blood-brain barrier that complicates efficient transmission of medication into the brain. Nanomaterials have a potential to upgrade therapeutic approaches because of their ability to pass through the blood-brain barrier, to improve pharmacological profile and safety of drugs and to transport drugs to the targeted area. Selenium nanoparticles stabilized by polyvinylpyrrolidone and gold nanoparticles stabilized by peptidoglycan monomer are examined as carriers for the levodopa transmission, with the goal of achieving the neuroprotective effect. Their ability to reduce oxidative stress caused by levodopa is evaluated in an in vivo model of Parkinson’s disease. Rats of both sexes were distributed in 7 groups and tested with test subtances for 28 days. The distribution of animals to the groups was random. Oxidative stress is examined by measuring reactive oxygen species and concentration of glutathione in brain, kidney, liver and serum of non-treated and treated rats. Results indicate a neuroprotective impact of selenium nanoparticles. Gold nanoparticles did not provide protective effect and it is possible that their application has a negative toxicological effects
The effect of cytokines TNF-alpha and IFN-gamma on REL, CXCL10 and PD-L1 gene expression in Farage and U2940 cell lines
No full textStanice imunosnog sustava posredstvom specifičnih receptora i signalnih molekula citokina reguliraju i aktiviraju imunosni odgovor. Aktivacijom raznih signalnih putova dolazi do aktivacije i diferencijacije imunosnih stanica u zrele i efektorske oblike. Faktor tumorske nekroze alfa (TNF-α, od engl. tumour necrosis factor) aktivira signalni put NF-κB, regulira ekspresiju posredničkih molekula i imunosnim stanicama omogućuje migraciju. Interferon gama (IFN-γ) aktivira signalni put JAK-STAT, utječe na ekspresiju kemokina i molekula glavnog sustava tkivne podudarnosti (MHC, od engl. major histocompatibility complex) te na aktivaciju imunosnih stanica. Procesi diferencijacije imunosne stanice čine tolerantnijima prema nakupljanju promjena u genomu i nepravilnoj transformaciji, koja može dovesti do nastanka limfoma. Primarni medijastinalni B-stanični limfom (PMBL) spada u skupinu ne-Hodgkinovih limfoma (NHL), a nastaje od B-limfocita smještenih u medijastinumu. U ovom radu istražen je utjecaj citokina TNF-α i IFN-γ na razinu ekspresije gena REL, CXCL10 i PDL1 u modelnim staničnim linijama. Gen REL kodira protein c-Rel, transkripcijski faktor signalnog puta NF-κB. Gen CXCL10 kodira istoimeni kemokin. Gen PDL1 kodira protein koji sudjeluje u mehanizmu koji regulira odgovor T-limfocita. Tretman citokinom IFN-γ povećavao je ekspresiju gena CXCL10 kod obje stanične linije, a ekspresiju gena PDL1 samo kod jedne stanične linije. Tretmani citokinom TNF-α pokazali su mješovite rezultate između staničnih linija za gen CXCL10 te većinom uzrokovali smanjenje ekspresije gena PDL1 i REL. Varijabilnost ekspresije istraživanih gena ukazuje na veliku heterogenost primarnog medijastinalnog limfoma.Immune cells regulate the immune response by interacting with specific receptors expressed on their surface and by secreting cytokines. Tumour necrosis factor alpha (TNF-α) activates the NF-κB signalling pathway, regulates the expression of mediators and enables the migration of immune cells. Interferon-gamma (IFN-γ) activates the JAK-STAT signalling pathway and influences the expression of chemokines and proteins of the major histocompatibility complex (MHC) and the activation of immune cells. The processes that immune cells undergo during their differentiation can contribute to abnormal transformations and the formation of lymphomas. Primary mediastinal large B-cell lymphoma (PMBL), a form of non-Hodgkin's lymphoma (NHL), arises from B-cells located in the mediastinum. The aim of this study was to investigate the effect of cytokines TNF-α and IFN-γ on the expression of REL, CXCL10 and PDL1 genes in PMBL model cell lines. The REL gene encodes the c-REL transcription factor of the NF-κB signalling pathway. The CXCL10 gene codes for a chemokine. The PDL1 gene codes for a protein that plays a decisive role in the regulatory mechanism of the T-cell immune response. IFN-γ caused an increase in CXCL10 gene expression in both cell lines and PDL1 gene expression in one cell line. TNF-α, on the other hand, showed ambiguous results for the CXCL10 gene and predominately caused a decrease in gene expression of PDL1 and REL genes. The variability of the expression of the investigated genes indicates the significant heterogeneity of primary mediastinal lymphoma
Synthesis of new aromatic C-nitroso derivatives and their adsorption on gold surface
No full textU ovom radu provedena je detaljna optimizacija sintetskih postupaka za pripravu novih aromatskih C-nitrozo derivata, koji sadrže funkcijsku skupinu sa sumporom koja im omogućuje adsorpciju na površinu zlata. U nekoliko reakcijskih koraka uspješno su sintetizirani i okarakterizirani bis(4-nitrozobenzil)disulfid i 1,2-bis(4-nitrozobifenil)disulfid. Aromatski C- nitrozo produkti su u posljednjem koraku sinteze pripravljeni redukcijom odgovarajućeg nitro derivata do N-hidroksilamina, koji je potom bez izolacije oksidiran do nitrozo spoja. Provedena su preliminarna istraživanja morfoloških i topografskih karakteristika molekularnih slojeva sintetiziranih aromatskih C-nitrozo derivata na površini zlata(111) uz pomoć mikroskopije atomskih sila. Dobiveni rezultati ukazuju na utjecaj specifičnih strukturnih karakteristika adsorbiranih nitrozo spojeva na njihovo samoudruživanje i dimerizaciju ili polimerizaciju na površini zlata.In this thesis, synthetic procedures for preparation of new aromatic C-nitroso derivatives containing a sulfur functional group for the adsorption on gold surface, were optimized in detail. In several reaction steps, bis(4-nitrosobenzyl)disulfide and 1,2-bis(4-nitrosobiphenyl)disulfide were successfully synthesized and characterized. The aromatic C-nitroso products were prepared in the final step of synthesis pathway by reducing the corresponding nitro derivative to N-hydroxylamine, which was then oxidized to the nitroso compound without isolation. Preliminary investigations of the morphological and topographical characteristics of molecular layers of synthesized aromatic C-nitroso derivatives on gold(111) surface were conducted using atomic force microscopy. The obtained results indicate the influence of specific structural characteristics of adsorbed nitroso compounds on their self-assembly and dimerization or polymerization on the gold surface
Položaj kromosoma 1 i 9, obilježenih pomoću tehnologije CRISPR/Cas9, u odnosu na jezgrinu ovojnicu i centrosome
No full textHuman chromosomes occupy specific territories in the cell nucleus. Chromosomes that are
localized at the periphery of the cell nucleus at the onset of mitosis show increased missegregation
rates, in compromised mitosis conditions, than the chromosomes that are
localized centrally. I specifically labeled subtelomeric region of chromosome 1 and
pericentromeric region of chromosome 9 using technology CRISPR/Cas9 (clustered regularly
interspaced short palindromic repeats and CRISPR-associated protein 9). I determined their
position in the cell nucleus, during early prometaphase, with respect to the nuclear envelope
and centrosomes. Chromosome 1 is localized at the periphery, whereas chromosome 9 is
equally distributed in the cell nucleus during early prometaphase. Furthermore, chromosome 1
and chromosome 9 are both equally polar (closing the angle of >90º with the spindle pole)
during early prometaphase. Also, there is no difference in the distance between chromosome 1
and chromosome 9 from the centrosome in early prometaphase and the average distance of
the centrosome to the former nuclear envelope in early prometaphase is 2.79 μm. The position
of centrosomes during early prometaphase is important from the point of mitotic fidelity. The
results of this thesis further support future research of specific chromosome position during
early prometaphase.Kromosomi ljudskih stanica zauzimaju specifične teritorije u jezgri. Kromosomi koji se
nalaze na periferiji jezgre prije početka mitoze skloniji su pogrešnoj podjeli, u uvjetima
narušene mitoze, u odnosu na kromosome koji su pozicionirani centralno. Specifično sam
obilježila subtelomernu regiju kromosoma 1 i pericentromernu regiju kromosoma 9
upotrebom tehnologije CRISPR/Cas9 (engl. clustered regularly interspaced short palindromic
repeats and CRISPR-associated protein 9). Odredila sam položaj navedenih kromosoma u
jezgri, tijekom rane prometafaze, u odnosu na jezgrinu ovojnicu i centrosome. Kromosom 1
zauzima periferni položaj, dok je kromosom 9 podjednako distribuiran u svim dijelovima
jezgre tijekom rane prometafaze. Nadalje, kromosom 1 i kromosom 9 su podjednako polarni
(zatvaraju kut >90º s polom diobenog vretena) tijekom rane prometafaze. Također, ne postoji
razlika u udaljenosti kromosoma 1 i kromosoma 9 od centrosoma u ranoj prometafazi te
prosječna udaljenost centrosoma od nekadašnje jezgrine ovojnice tijekom rane prometafaze
iznosi 2,79 μm. Pozicija centrosoma tijekom rane prometafaze važna je za pravilnu diobu.
Rezultati proizašli iz ovoga rada podupiru buduća istraživanja položaja specifičnih
kromosoma tijekom rane prometafaze
The development of cultural and creative industries as a catalyst for urban regeneration in Trnje
No full textUrbana regeneracija vrlo je kompleksan proces koji zauzima sve važnije mjesto u razvoju postindustrijskih gradova. Ona nastoji dovesti do poboljšanja gospodarskih, društvenih, fizičkih i okolišnih uvjeta u zapuštenim područjima. Postoje različiti pristupi urbanoj regeneraciji, a u ovom radu je istražen pristup kroz razvoj i djelovanje kulturnih i kreativnih industrija. Kulturne i kreativne industrije važan su sektor u novoj ekonomiji koji nudi različite usluge i mogućnosti za razvoj gradova – bilo to u kontekstu povećanja gospodarske konkurentnosti, poticanju društvenog i kulturnog razvoja ili oblikovanju fizičkih elemenata u prostoru. Prostorni obuhvat istraživanja je administrativno područje zagrebačke gradske četvrti Trnje, s fokusom na tzv. brownfield područjima. U radu su istražene prilike koje nudi urbana regeneracija temeljena na razvoju kulturnih i kreativnih industrija, uz identifikaciju izazova koji se pojavljuju u njenom planiranju. Konzultacijom relevantne znanstvene i stručne literature te istraživačkom metodom intervjua dobiveni su rezultati prema kojima su određena načela i uvjeti za kvalitetno planiranje urbane regeneracije u GČ Trnje, s naglaskom na kulturnu komponentu.Urban regeneration is a highly complex process that is becoming increasingly important in the development of post-industrial cities. It aims to improve the economic, social, physical, and environmental conditions in neglected areas. There are various approaches to urban regeneration, and this paper explores the approach based on the development and operation of cultural and creative industries. Cultural and creative industries are a key sector in the new economy, offering a range of services and opportunities for urban development – whether through enhancing economic competitiveness, fostering social and cultural growth, or shaping the physical elements of urban space. The spatial scope of the research is the administrative area of the Trnje district in Zagreb, with a focus on so-called brownfield sites. The paper examines the opportunities offered by urban regeneration based on the development of cultural and creative industries, while also addressing the challenges that arise in its planning. Through a review of relevant scientific and professional literature and the use of the interview research method, the findings provided a basis for identifying principles and conditions for high-quality urban regeneration planning in the Trnje district, with an emphasis on the cultural component