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”Tämän päivän yhteiskunnasta säilyy vain satunnaisia tietoja tuleville tutkijasukupolville” – Vähimmäissäilytysaikojen muutokset sekä sähköisen ja paperisen säilytyksen vastakkainasettelu Liikearkistoyhdistyksen julkaisuissa 1994–2019
No full textSuositukset jakamistalouden edistämiseksi. Jaa Se! – Parhaat vinkit ja lempeä tuuppaus jakamistalouteen -hanke
No full textKuluttajien arjen valinnoilla on keskeinen rooli kuluttamisen ilmasto- ja ympäristövaikutusten hillinnässä. Kestävään elämäntapaan siirtymisen tulisi olla kaikille saavutettava, houkutteleva ja sujuvasti omaksuttava vaihtoehto.
Jakamistalous, joka perustuu kuluttajien yhteistoimintaan, tavaroiden tehokkaampaan kiertoon ja käyttöasteen nostamiseen, tarjoaa konkreettisen keinon kestävämmän arjen rakentamiseen. Jakamistalouden potentiaalin hyödyntäminen edellyttää kuitenkin pitkäjänteistä työtä, jossa tunnistetaan ja puretaan rakenteellisia esteitä, asenteellisia haasteita sekä eriarvoisuutta lisääviä tekijöitä
Toxicological Effects of Anabolic-Androgenic Steroids: Whole-Body Impact and Health Risks
No full textMicroRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution
No full textBackground
Regulatory networks controlling aging and disease trajectories remain incompletely understood. MicroRNAs (miRNAs) are a class of regulatory non-coding RNAs that contribute to the regulation of tissue homeostasis by modulating the stability and abundance of their target mRNAs. MiRNA genes are transcribed similarly to protein-coding genes which has facilitated their annotation and quantification from bulk transcriptomes. Here, we show that droplet, spatial, and plate-based single-cell RNA-sequencing platforms can be used to decipher miRNA gene signatures at cellular resolution to reveal their expression dynamics in vivo.
Methods
We first benchmarked the approach examining concordance between platforms, species, and cell type-specific bulk expression data. To discover changes in miRNA gene expression that could contribute to the progressive loss of cellular homeostasis during aging and disease development, we annotated the comprehensive aging mouse dataset, Tabula Muris Senis, with cell type-specific miRNA expression and acquired transcriptome and translatome profiles from an atherosclerosis disease model.
Results
We generated an openly available workflow and aging-profile resource to characterize miRNA expression from single-cell genomics studies. Comparing immune cells in spleen tissue between young and old mice revealed concordance with previous functional studies, highlighting the upregulation of mmu-mir-146a, mmu-mir-101a, and mmu-mir-30 family genes involved in senescence and inflammatory pathways. Atherosclerosis progression is reflected within adipose tissue as expansion of the myeloid compartment, with elevated pro-inflammatory mmu-mir-511 expression in several macrophage subtypes. Upregulation of the immunosuppressive mmu-mir-23b ~ mir-24–2 ~ mir-27b locus was specific to Trem2 + lipid-associated macrophages, prevalent at late disease. Accordingly, ribosome-associated RNA profiling from myeloid cells in vivo validated significant mmu-mir-23b target gene enrichment in disease-regulated translatomes. Prominent tissue infiltration of monocytes led to upregulated mmu-mir-1938 and mmu-mir-22 expression and in classical monocytes activated mmu-mir-221 ~ 222, mmu-mir-511, and mmu-mir-155 gene loci, confirmed by bulk nascent transcriptomics data from ex vivo macrophage cultures. Overall, the monocyte-associated changes in miRNA expression represented the most significant target gene associations in the disease-trajectory translatome profiles.
Conclusions
We demonstrate that miRNA gene transcriptional activity is widely impacted in immune cells by aging and during disease development and further identify the corresponding translatome signature of inflamed adipose tissue
Engineering biomimetic zonal properties in fibre-reinforced hydrogels for functional cartilage tissue engineering in vitro
No full textThe functional regeneration of human articular cartilage is hampered by a lack of biomaterials and tissue engineering strategies that adequately capture the physiological depth-dependent compression properties of native tissue. Here, we demonstrate that photocrosslinkable gelatin-hyaluronic acid hydrogels reinforced with multiphasic polycaprolactone microfibre scaffolds form biomimetic soft network composites that serve as in vitro models mimicking the compressive and depth-dependent deformation characteristics of human cartilage. Mono- and multi-phasic gradient scaffolds with fibre spacings of 200, 400, and 800 μm were manufactured using melt electrowriting and embedded in the photocrosslinkable hydrogel system. Mechanical testing combined with finite element analysis revealed how defined microfibre architecture is altered and influences compressive moduli and interstitial fluid load support to mimic the loading response of native articular cartilage in our in vitro model. Digital image and volume correlations demonstrated depth-dependent strain fields in the fibre-reinforced constructs in response to compression, demonstrating biomimetic depth-dependent behaviour. Lastly, we demonstrate that these fibre-reinforced hydrogels support high cell viability, chondrogenic redifferentiation and hyaline-like tissue formation by expanded human articular chondrocytes in vitro. Together, this study demonstrates that, in vitro, these hydrogels reinforced with gradient scaffolds successfully recapitulate key biomechanical traits of native articular cartilage, toward the development of improved models for functional cartilage tissue engineering
Yliopisto-opettajien näkökulma kulttuurisesti responsiivisen pedagogiikan (CRP) toteuttamiseen suomalaisissa luokkahuoneissa: haasteet ja mahdollisuudet
No full textDevelopment and Management of Digitalisation in the Evangelical Lutheran Church of Finland
No full text
