HAL du Programme national de recherche environnement-santé-travail (PNR EST)
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Comparison of a radiofrequency electric and magnetic field source-based job-exposure matrix with personal radiofrequency exposure measurements
No full textInternational audienceOBJECTIVES: Assessing occupational exposure to radiofrequency electromagnetic fields (RF-EMF) presents significant challenges due to the considerable variability in exposure levels within and between occupations. This spatial and temporal variability complicates the reliable evaluation of potential health risks associated with RF-EMF exposure in the workplace. Accurate assessment methods are crucial to understand the extent of exposure and to evaluate potential health risks, especially given the potential for higher exposures in occupational settings compared to the general population. This study compares the historical RF-EMF exposure estimates in the INTEROCC RF-EMF job-exposure matrix (RF-JEM) with recent personal measurement data collected in 2 countries as part of the OccRF-Health study, to assess the broader applicability of the RF-JEM. METHODS: Weighted kappa (k w) coefficients and Spearman rank correlation tests were performed to assess the alignment between RF-JEM estimates and measurements for 8 h time-weighted average exposure intensity and prevalence estimates across various occupations. The comparisons were mainly based on 22 jobs having ≥5 measured workers in the OccRF-Health study. RESULTS: Poor agreement was found for both exposure prevalence and intensity between both methods (k w < 0.1). RF-JEM values likely overestimated exposure levels for both electric (E) and magnetic (H) fields (mean percentage difference >194%) compared to current personal measurements. CONCLUSIONS: Findings suggest that the INTEROCC-JEM likely overestimates current exposure intensity levels in the measured jobs. Adopting a semiquantitative JEM could also mitigate misclassification errors due to exposure variability, improving accuracy in exposure assessment. These findings indicate the need for more targeted personal measurements, including among highly exposed workers, and for potentially considering new exposure metrics to more accurately assess occupational RF-EMF exposures in occupational epidemiological research
Characterization of the metabolomic response of freshwater periphytic biofilm exposed to a serial dilution of glyphosate
No full textInternational audienceGlyphosate is a widely used herbicide detected ubiquitously in European freshwaters. However, there still is a lack of knowledge about its impact on aquatic ecosystems. To fill this gap, periphytic community playing a pivotal role in ecosystem functions, is a relevant model. Also, untargeted meta-metabolomics is an approach of choice through its ability to sensitively characterize the molecular/biochemical phenotype of the community. In this context, this study aims to characterize the meta-metabolome response of periphytic biofilm exposed during 14 days to different concentrations of glyphosate (0.1-150 µg.L-1). The meta-metabolome was characterized by an untargeted approach on a UPLC-HRMS-TOF system. Acquired data were processed in W4M and further analyzed with various chemometric methods. In particular, DROMICS was used to characterize trend (U, Bell, Increase, Decrease) and sensitivity (BMD1sd) of each metabolomic features as well as their aggregated response. Finally, the signals of interest were annotated by combining MS-DIAL and SIRIUS5. Chemometrics revealed that 7276 features reacted to glyphosate. Only 2288 could be annotated. Among them alkaloids (n=662) and amino acids and peptides (n=952) classes were the more reactive. Surprisingly, low effect of glyphosate was noted on the shikimates while this herbicide targets their biosynthesis. DROMICS showed no discrepancy in the proportion of each trend and the sensitivity to glyphosate between the classes. However, the strengh of the response (i.e. fold change) slightly change between the classes, as terpenoids and amino-acids were the most down-regulated classes. Altogether, our results higlighted a complex metabolic response of periphyton to glyphosate that questions potential impairment of ecological functions of these communities
Fetal and Infancy Exposure to Phenols, Parabens, and Phthalates and Anthropometric Measurements up to 36 Months, in the Longitudinal SEPAGES Cohort
No full textInternational audienceBACKGROUND: Endocrine-disrupting chemicals may play a role in adiposity development during childhood. Until now literature in this scope suffers from methodologic limitations in exposure assessment using one or few urine samples and missing assessment during the infancy period.OBJECTIVES: We investigated the associations between early-life exposure to quickly metabolized chemicals and post-natal growth, relying on repeated within-subject urine collections over pregnancy and infancy.METHODS: We studied the associations of four phenols, four parabens, seven phthalates, and one nonphthalate plasticizer from weekly pooled urine samples collected from the mother during second and third trimesters (median 18 and 34 gestational weeks, respectively) and infant at2 and 12 months of age, and child growth until 36 months. We relied on repeated measures of height, weight and head circumference from study visits and thechild health booklet to predict growth outcomes at 3 and 36 months using the Jenss-Bayley nonlinear mixed model. We assessed associations with individual chemicals using adjusted linear regression and mixtures of chemicals using a Bayesian kernel machine regression model.RESULTS: The unipollutant analysis revealed few associations. BisphenolS (BPS) at second trimester was positively associated with all infant growth parameters at 3 and 36 months, with similar patterns between exposure at third trimester and all infant growth parameters at 3 months. Mono-n-butyl phthalate (MnBP) at 12 months was positively associated with body mass index (BMI), weight, and head circumference at 36 months. Mixture analysis revealed positive associations between exposure at 12 months and BMI and weight at 36 months, with MnBP showing the highest effect size within the mixture.CONCLUSIONS: This study suggests that exposure in early infancy may be associated with increased weight and BMI in early childhood, which are risk factors of obesity in later life. Furthermore, this study highlighted the impact of BPS, a compound replacing bisphenol A, which has never been studied in this context
238MO Long-term residential and workplace exposure to air pollution and breast cancer risk: A case-control study nested in the French E3N cohort from 1990 to 2011
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Bioaccumulation et effets des perturbateurs endocriniens sur la luzerne et la tomate: Bioaccumulation et effets des perturbateurs endocriniens sur la luzerne et la tomate Dynamique de 2 perturbateurs endocriniens chez 2 plantes cultivées et évaluation de leurs effets sur un insecte polyphage et la souris
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Identification des protéines cibles des perturbateurs endocriniens: Identification de protéines Partenaires de perturbateurs Endocriniens par protéolyse limitée et spectRométrie de masse
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Occupational exposure to aerosols in two French airports: multi-year lung function changes
No full textInternational audienceAbstract As differential exposure to airport-generated aerosols may affect employee lung function, the main objective of this study was to longitudinally evaluate spirometry measures among Air France employees. In addition, an exploratory exposure assessment to airport aerosol was performed in a small cohort of workers using personal monitoring devices. Change in lung function over a ~6.6-yr period was documented for office workers (n = 68) and mechanics (n = 83) at Paris-Roissy airport, France and terminal (n = 29), or apron (n = 35) workers at Marseille airport, France. Overall, an excessive decline in lung function was found for 24.75% of airport workers; excessive decline occurred more often for terminal workers (44.83%) as compared to mechanics (14.47%; P = 0.0056), with a similar tendency for apron workers (35.29%) as compared to mechanics (P = 0.0785). Statistically significant differences/tendencies were detected among the yearly rates of change for %-predicted values of forced expiratory volume in 1 s, forced vital capacity, peak expiratory flow, and from 25% to 75% forced expiratory flow. For the latter variables, the terminal and/or apron workers at Marseille generally had significantly faster lung function decline as compared to office workers and/or mechanics in Paris, although the latter were exposed to a higher level of elemental carbon. No relation between lung function decline and exposure to airport tarmac environments was evidenced. Multivariate exploration of individual variables representing sex, smoking, atopy, respiratory disease, residential PM2.5 pollution, the peak size of particles in lung exhalates or exhaled carbon monoxide at the time of follow-up failed to explain the observed differences. In conclusion, this study documents the first evidence of excessive lung function decline among certain airport workers in France, although the identification of emission sources (environmental factors, aircraft exhaust, etc) remains challenging
Phototoxicity of low doses of light and influence of the spectral composition on human RPE cells
No full textInternational audienceLight is known to induce retinal damage affecting photoreceptors and retinal pigment epithelium. For polychromatic light, the blue part of the spectrum is thought to be the only responsible for photochemical damage, leading to the establishment of a phototoxicity threshold for blue light (445 nm). For humans it corresponds to a retinal dose of 22 J/cm2. Recent studies on rodents and non-human primates suggested that this value is overestimated. In this study, we aim at investigating the relevance of the current phototoxicity threshold and at providing new hints on the role of the different components of the white light spectrum on phototoxicity. We use an in vitro model of human induced pluripotent stem cells (hiPSC)-derived retinal pigment epithelial (iRPE) cells and exposed them to white, blue and red lights from LED devices at doses below 22 J/cm2. We show that exposure to white light at a dose of 3.6 J/cm2 induces an alteration of the global cellular structure, DNA damage and an activation of cellular stress pathways. The exposure to blue light triggers DNA damage and the activation of autophagy, while exposure to red light modulates the inflammatory response and inhibits autophagy
Development of an in vitro biofilm model for the study of the impact of fluoroquinolones on sewer biofilm microbiota
No full textInternational audienceSewer biofilms are likely to constitute hotspots for selecting and accumulating antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). This study aimed to optimize culture conditions to obtain in vitro biofilms, mimicking the biofilm collected in sewers, to study the impact of fluoroquinolones (FQs) on sewer biofilm microbiota. Biofilms were grown on coupons in CDC Biofilm Reactors®, continuously fed with nutrients and inoculum (1/100 diluted wastewater). Different culture conditions were tested: (i) initial inoculum: diluted wastewater with or without sewer biofilm, (ii) coupon material: concrete vs. polycarbonate, and (iii) time of culture: 7 versus 14 days. This study found that the biomass was highest when in vitro biofilms were formed on concrete coupons. The biofilm taxonomic diversity was not affected by adding sewer biofilm to the initial inoculum nor by the coupon material. Pseudomonadales, Burkholderiales and Enterobacterales dominated in the sewer biofilm composition, whereas in vitro biofilms were mainly composed of Enterobacterales . The relative abundance of qnrA, B, D and S genes was higher in in vitro biofilms than sewer biofilm. The resistome of sewer biofilm showed the highest Shannon diversity index compared to wastewater and in vitro biofilms. A PCoA analysis showed differentiation of samples according to the nature of the sample, and a Procrustes analysis showed that the ARG changes observed were linked to changes in the microbial community. The following growing conditions were selected for in vitro biofilms: concrete coupons, initial inoculation with sewer biofilm, and a culture duration of 14 days. Then, biofilms were established under high and low concentrations of FQs to validate our in vitro biofilm model. Fluoroquinolone exposure had no significant impact on the abundance of qnr genes, but high concentration exposure increased the proportion of mutations in gyr A (codons S83L and D87N) and par C (codon S80I). In conclusion, this study allowed the determination of the culture conditions to develop an in vitro model of sewer biofilm; and was successfully used to investigate the impact of FQs on sewer microbiota. In the future, this setup could be used to clarify the role of sewer biofilms in disseminating resistance to FQs in the environment
Experimental investigation of the effect of tebuconazole on three biomarkers of innate immunity in the house sparrow (Passer domesticus)
No full textInternational audienceTriazoles are among the most widely used fungicides in the world due to their efficacy against fungal crop diseases and their broad spectrum of action. Intensive use of triazoles has resulted in residual contamination in different compartments of agroecosystems and exposes non-target species to potential sublethal effects. Triazoles are known to be immunomodulators in medicine and therapeutic treatments, but very little data is available on their potential effect on immune parameters of non-target vertebrate species living in agroecosystems. In this study, we experimentally examined the impact of tebuconazole on three immune biomarkers (haemagglutination titre (HA), haemolysis titre (HL), and haptoglobin concentration (Hp)), as well as on the body condition of house sparrows (Passer domesticus). Our results suggest that tebuconazole had very little, if any, effect on the studied immune parameters. However, further studies are needed to better assess the effect of tebuconazole on bird immunity because (1) experimental individuals were kept under optimal conditions and the impact of tebuconazole on immunity may occur under suboptimal conditions, (2) only one concentration of tebuconazole was tested and its effect could be dose-dependent and (3) other complementary immunological biomarkers should be studied, given the complexity of the vertebrate immune system. Current knowledge on the potential effects of triazoles on the immunity of wild farmland vertebrates is still largely insufficient. Further physiological and immune studies should be conducted to better understand the effect of triazole fungicides on farmland birds