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Tuning the porosity of biofabricated membrances in microfluidics with incorporated nanoparticles as template
No full textby Khanh Loan Ly.Typescript.Thesis (M.S.E.--Biomedical)--Catholic University of America, 2020.Bibliography: leaves 47-48.Also available in microfilm
Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens
No full textProceedings of the National Academy of Sciences of the United States of America, Volume 117, Issue 5, 4 February 2020, Pages 2613-2621.Tsetse-transmitted African trypanosomes must develop into mammalian-infectious metacyclic cells in the fly’s salivary glands (SGs) before transmission to a new host. The molecular mechanisms that underlie this developmental process, known as metacyclogenesis, are poorly understood. Blocking the few metacyclic parasites deposited in saliva from further development in the mammal could prevent disease. To obtain an in-depth perspective of metacyclogenesis, we performed single-cell RNA sequencing (scRNA-seq) from a pool of 2,045 parasites collected from infected tsetse SGs. Our data revealed three major cell clusters that represent the epimastigote, and pre- and mature metacyclic trypanosome developmental stages. Individual cell level data also confirm that the metacyclic pool is diverse, and that each parasite expresses only one of the unique metacyclic variant surface glycoprotein (mVSG) coat protein transcripts identified. Further clustering of cells revealed a dynamic transcriptomic and metabolic landscape reflective of a developmental program leading to infectious metacyclic forms preadapted to survive in the mammalian host environment. We describe the expression profile of proteins that regulate gene expression and that potentially play a role in metacyclogenesis. We also report on a family of nonvariant surface proteins (Fam10) and demonstrate surface localization of one member (named SGM1.7) on mature metacyclic parasites. Vaccination of mice with recombinant SGM1.7 reduced parasitemia early in the infection. Future studies are warranted to investigate Fam10 family proteins as potential trypanosome transmission blocking vaccine antigens. Our experimental approach is translationally relevant for developing strategies to prevent other insect saliva-transmitted parasites from infecting and causing disease in mammalian hosts
Determining the modification of parishes and changing the status of Church edifices inthe diocese of Grand Island: overview and recommendations
No full textby Vincent L. Parsons.Typescript.Thesis (J.C.L.)--Catholic University of America, 2019.Bibliography: leaves 48-51.Also available in microfilm.Also available in microfiche
DC Public Library staff member Biljana
No full textDC Public Library staff member Biljana wears a bicycle-themed mask and holds up her bicycle in front of the Cleveland Park Library
Two men steam crabs at American Ice Company
No full textTwo men steam crabs at American Ice Company on V Street NW. They both wear face coverings and gloves
WORKING MEMORY CAPACITY: HOW COGNITIVE ABILITIES INFLUENCE POST-MESSAGE ATTITUDES IN AN ADVERTISING CONTEXT
No full textWorking memory capacity (WMC) reflects natural variability in individuals’ ability to maintain attention on a goal state or task in the face of interference from non-task relevant stimuli. Individuals with a lower-WMC experience higher rates of mind-wandering and increased fluctuations in task engagement, when compared to higher-WMC individuals. Although the use of cognitive theories is common to explain the processes that underlie advertising effects, research focused on how cognitive abilities, such as working memory capacity, impact the processing and outcomes of persuasive communications remains scant. The current study uses a classic consumer behavior framework to assess advertising outcomes, such as brand attitude, as a result of exposure to a persuasive communication; additionally, I include a pupillometry analysis that provides additional considerations on differences between higher- vs lower-capacity individuals. The results indicate that the interaction between brand name placement (first or last in a commercial) and working memory capacity on brand attitudes was marginally significant. The pattern of effects is such that for lower-WMC individuals, brand attitudes were most favorable when the brand name was revealed at the beginning of a commercial; while, brand name placement was not significantly related to brand attitudes for average and higher-WMC individuals. The pupillometry results demonstrate that for higher-WMC individuals, pupil diameter was the largest when the brand name is revealed last, and brand name placement was not related to pupil diameter for average or lower-WMC individuals. While the brand name placement and working memory interaction was significant for pupil diameter, there was no significant interaction for pupil variability. There was, however, a main effect of working memory capacity on pupil variability, such that as working memory capacity increased, pupil variability decreased. This thesis is the first study to show these pupillometry results in an applied context. The discussion presents limitations of the current study and provides considerations for future research.PsychologyExperimental psychologyadvertising, cognitive abilities, intentions, pupillometry, working memory capacityPsychologyDegree Awarded: M.A. Psychology. American Universit
Thank you sign on 13th Street SE
No full textThank you sign on the front door of a house on 13th Street SE. The sign features a picture of a healthcare worker wearing a mask
Communitas Capax Legis Recipiendæ: The Parish Communitas of c. 515 §1 and Parochial Capacity in cc. 25 and 29
No full textThe Parish Communitas of c. 515 §1 and Parochial Capacity in cc. 25 and 29Canon lawCanon 25, Canon 29, Canon 515, Community capable of receiving law, Custom, Treatise on LawCanon LawDegree Awarded: J.C.L. Canon Law. The Catholic University of Americ
The Possibility of Moral Science in the Philosophy of William Ockham
No full textThe essential character of William of Ockham’s moral theory has long been an issue in the secondary literature due to the perceived difficulty of reconciling his claim that morals is a science based upon necessary principles with his equally strong claim that God can issue virtually any command which it would be our moral duty to obey. Most commentators have attempted to resolve this difficulty by focusing on a select group of texts in which Ockham makes these claims, but other texts in which he deals with these issues, sometimes more directly, often have been ignored. The author of the present dissertation attempts to resolve this issue by means of a close reading of all of the relevant texts in Ockham’s works. In the first chapter, the author presents the primary texts of Ockham which have been the focus of discussion; he then surveys the secondary literature, and offers criticisms of the dominant positions that have been taken. In the second chapter the author examines Ockham’s views concerning the nature and requirements for scientific knowledge, especially that it must be in the form of a demonstration; and in the third chapter, he more fully examines one of these requirements, that all of the propositions in a scientific demonstration must be necessary propositions. Having established the requirements for scientific knowledge in general, in the fourth chapter the author attempts to establish that Ockham’s moral theory can yield scientific knowledge of ethical matters even if such knowledge is limited to general propositions as opposed to propositions regarding particular actions. In the fifth chapter, he extends this analysis to Ockham’s natural law theory as presented in his political writings. He shows that, despite a significant terminological difference between Ockham’s earlier academic writings and his political writings, the same tensions are equally present in the latter as in the former, and so the resolution offered in the previous chapter can easily be extended to Ockham’s natural law theory in his political writings. The final chapter sums up the results of thePhilosophyMoral, Ockham, SciencePhilosophyDegree Awarded: Ph.D. Philosophy. The Catholic University of Americ
Investigating Japanese "Heritage for Diplomacy": An Analysis of Local Narratives at Controversial UNESCO World Heritage Sites in Japan
No full textThis thesis is a response to the dearth of research on the impact of subnational factors in heritage diplomacy by comparing local and national narratives at controversial UNESCO World Heritage Sites in Japan. In addition to expanding on existing literature, the stated research goals include developing a new theoretical framework through which to better understand and analyze the role of domestic actors and actions in heritage diplomacy practice. The research was conducted on-site in Japan and utilizes a qualitative methodology rooted in content and narrative analysis. Of the many conclusions drawn, the most significant finding stresses the importance of consistent heritage messaging at the national and subnational level as a key factor in establishing and maintaining credibility for heritage diplomacy strategies conducted on the international stage.International relationsCultural resources managementAsian studiesCultural heritage, Diplomacy, Japan, Narrative analysis, UNESCOSchool of International ServiceDegree Awarded: M.A. School of International Service. American Universit