Naresuan University Journal
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Integrated multi-omics analyses reveal the pleiotropic nature of the control of gene expression by Puf3p.
No full textThe PUF family of RNA-binding proteins regulate gene expression post-transcriptionally. Saccharomyces cerevisiae Puf3p is characterised as binding nuclear-encoded mRNAs specifying mitochondrial proteins. Extensive studies of its regulation of COX17 demonstrate its role in mRNA decay. Using integrated genome-wide approaches we define an expanded set of Puf3p target mRNAs and quantitatively assessed the global impact of loss of PUF3 on gene expression using mRNA and polysome profiling and quantitative proteomics. In agreement with prior studies, our sequencing of affinity-purified Puf3-TAP associated mRNAs (RIP-seq) identified mRNAs encoding mitochondrially-targeted proteins. Additionally, we also found 720 new mRNA targets that predominantly encode proteins that enter the nucleus. Comparing transcript levels in wild-type and puf3∆ cells revealed that only a small fraction of mRNA levels alter, suggesting Puf3p determines mRNA stability for only a limited subset of its target mRNAs. Finally, proteomic and translatomic studies suggest that loss of Puf3p has widespread, but modest, impact on mRNA translation. Taken together our integrated multi-omics data point to multiple classes of Puf3p targets, which display coherent post-transcriptional regulatory properties and suggest Puf3p plays a broad, but nuanced, role in the fine-tuning of gene expression
Optimal cooperative relaying and jamming for secure communication
No full textThis letter considers joint cooperative beamforming and jamming schemes in a cooperative relay network where distributed relay nodes perform cooperative beamforming. The problem of interest is to keep the eavesdropper’s signal to interference and noise ratio (SINR) as low as possible while maintaining the legitimate receiver’s SINR requirement. Applying semidefinite relaxation (SDR) technique, it is shown that a rank-one optimal solution always exists if perfect channel information is available. An optimal relay beamforming scheme has also been proposed for the scenario where only the second-order statistics of the eavesdropper’s channel is available
Rapid selective separation of americium/curium from simulated nuclear forensic matrices using triazine ligands
No full textIn analysis of complex nuclear forensic samples containing lanthanides, actinides and matrix elements, rapid selective extraction of Am/Cm for quantification is challenging, in particular due the difficult separation of Am/Cm from lanthanides. Here we present a separation process for Am/Cm(III) which is achieved using a combination of AG1-X8 chromatography followed by Am/Cm extraction with a triazine ligand. The ligands tested in our process were CyMe4-BTPhen, CyMe4-BTBP, CA-BTP and CA-BTPhen. Our process allows for purification and quantification of Am and Cm (recoveries 80%–100%) and other major actinides in <2d without the use of multiple columns or thiocyanate. The process is unaffected by high level Ca(II)/Fe(III)/Al(III) (10mg mL−1) and thus requires little pre-treatment of samples
Quantitative determination of the spatial orientation of graphene by polarized Raman spectroscopy
No full textPolarized Raman spectroscopy has been employed to characterize transverse sections of graphene monolayers upon both a copper substrate and a polyester film. Well-defined Raman spectra can be obtained from the one atom thick transverse sections of graphene because of the strong resonance Raman scattering. The intensity of Raman 2D band (I-2D) is independent of the axis of laser polarization when the laser beam is perpendicular to the surface of the graphene monolayer but I-2D is found to vary as approximately the 4th power of the cosine of the angle between the axis of laser polarization and the plane of graphene when the direction of laser propagation is parallel to the graphene sheet. It is demonstrated that a generalized spherical expanded harmonics orientation distribution function (ODF) can be used to quantify the spatial orientation of the graphene. The roughness of the graphene, evaluated using atomic force microscopy, shows a good correlation with the ODF determined using polarized Raman spectroscopy, showing how the Raman technique may be employed to quantify the spatial orientation of graphene. It is also demonstrated how the technique can be used to quantify the orientation of graphene in high-ordered pyrolytic graphite and graphene paper. (c) 2015 The Authors. Published by Elsevier Ltd
Distinct clinical and pathological phenotypes in frontotemporal dementia associated with MAPT, PGRN and C9orf72 mutations.
No full textOur objective was to compare the clinical and pathological characteristics of frontotemporal dementia patients with MAPT, GRN and C9orf72 gene mutations. We carried out a cross-sectional comparative study of 74 gene-positive patients (15 MAPT, 17 GRN and 42 C9orf72). Thirty had post mortem pathological data permitting clinico-pathological correlation. MAPT patients were younger than other groups, and showed more frequent behavioural disinhibition, repetitive and stereotyped behaviours, semantic impairment and temporal predominance of atrophy. GRN patients were older at death and more likely to present with non-fluent aphasia. C9orf72 patients alone showed a co-occurrence of ALS. They showed more psychotic symptoms and irrational behaviour, yet were more often reported clinically as socially appropriate and warm. They showed less dietary change than other groups. C9orf72 patients with and without ALS differed only in frequency of psychosis. Greater clinical overlap was observed between GRN and C9orf72 compared to MAPT cases. MAPT cases had tau and GRN and C9orf72, with one exception, TDP-43 pathology. Non-fluent aphasia was linked to TDP subtype A in both GRN and C9orf72 cases and ALS with subtype B. In conclusion, the findings reinforce clinical heterogeneity in FTD and strengthen evidence that genotype influences clinical presentation. Clinical features may inform targeted genetic testing
