Veterinaria Italiana (Journal)
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Etiological agents and bacterial sensitivity in subclinical mastitis in Brazil: a ten-year systematic review
Get PDFConsidering the high prevalence of subclinical mastitis and its impacts on milk production, thematic studies are need to provide strategic data for its control. This study aimed at investigating the most frequent microorganisms associated with subclinical mastitis in dairy cows in Brazil through compiling the occurrence of the etiological agents and their sensitivity to antibiotics. The systematic review includes articles published between 2009 and 2019. Fiftyseven articles evaluating 22,287 milk samples were selected. The number of publications and the sample size were not homogeneous among Brazilian regions. Most of the studies and sampling were conducted in Rio Grande do Sul, whereas no studies were found in some states in the north and mid‑west regions. The most frequent pathogen was Staphylococcus spp. It was isolated in all studies and had an average prevalence of 49% in the analyzed samples. Resistance to penicillin was the most frequent microbial resistance found in Brazil, with an average of 66% among the isolates evaluated. Moreover, bacterial resistance to cephalexin, cefoperazone, erythromycin, gentamicin, neomycin, penicillin, tetracycline, and trimethoprim increased over the research period. Given the territorial extension, the etiological diversity, and the lack of studies with a representative sample, the compilation of scientific data must be interpreted with caution. Regions where a greater number of studies were conducted and with numerous samples, such as the South, provided a comprehensive scenario that is closer to reality. Nevertheless, although decision making on the farm cannot be replaced by scientific studies, it can be supported by such efforts
Marek’s disease in genetically susceptible Cochin chickens in Italy: a case report
Get PDFThe present study investigates an outbreak of classical Marek’s disease (MD) in backyard Cochin chickens reared for hobby in Italy. Examined chickens showed spastic paralysis of the legs and at necropsy, enlargement and discoloration of the peripheral nerves and plexuses that matched microscopic A‑ and B‑ type MD lesions. Molecular analysis of the meq gene of the detected Gallid alphaherpesvirus 2 (GaHV‑2) strain, showed typical markers of low virulence and the strain shared the entire meq gene sequence with strains circulating in Italian backyard chickens. Furthermore, the haplotype B19 of the major histocompatibility complex (MHC) was defined in the affected chickens, showing that the birds possessed a genetic profile of high susceptibility to MD, allowing the appearance of a classical nervous clinical form after infection with an apparently low pathogenicity GaHV‑2 strain. Trade of live ornamental purebred chickens occurs frequently between hobby farmers and biosecurity practices, such as quarantine periods, should be applied to avoid the introduction of infected animals. Veterinarians should raise awareness of this issue and promote the use of vaccines against MD
Prevalence and molecular characterization of typhoidal and non-typhoidal Salmonella isolated from meat and environmental samples of retail shops of Lahore Punjab, Pakistan
Get PDFNon-typhoidal Salmonellae are important foodborne bacterial pathogens that can cause bacteremia, gastroenteritis, and subsequent infection. The aim of the study was to determine the prevalence of Salmonella in the live bird market and retail shops of Lahore (Pakistan). A total of 720 samples of chicken meat, chopping board, cages, hands, and transportation vans were collected. Salmonella was recovered from 103 (14.36%) samples. The highest prevalence (33.33%) was found in transportation van samples followed by chicken meat samples (17.26%). In the towns of Lahore, the highest prevalence was found in Samanabad Town (19%) followed by Data Ganj Bakhsh Town (17%) with the lowest in Gulberg Town (6.9%). Salmonella Typhimurium was most common (35.92%) followed by S. Enteritidis (25.24%), S. Dublin (14.56%), S. Gallinarum biovar Gallinarum (8.74%), and untyped Salmonella species (15.53%). This was the first baseline study of the prevalence of non-typhoidal Salmonella at the live bird market and retail shops of Lahore. Implementation of appropriate control measures is required at both the human side and poultry food production chain to reduce the burden and transmission of the zoonotic Salmonellae
An outbreak of duck hepatitis A virus infection in nomadic ducklings
Get PDFDuring winter of the year 2020, a flock of 9 day‑old 5000 non‑descript ducklings was affected with huge daily mortality, dullness, depression and opisthotonus. Clinically, there was severe depression, spasmodic paddling and opisthotonus. On post‑mortem, liver was enlarged and pale with patchy ecchymoses. Presence of perihepatitis and pericardititis during post‑mortem examination of one duckling might be attributed to secondary bacterial infection. Upon completion of disease episode, there was 80 percent mortality in eight days and only less than 20 percent weak ducklings survived. Liver homogenate which was subjected for molecular confirmation through one‑step reverse transcriptase polymerase chain reaction (RT‑PCR) using primers for RNA dependent RNA polymerase (3D) gene yielded positivity for duck hepatitis A virus (DHAV‑1). Histological observation of liver revealed hepatocyte degeneration and necrosis. It is clear that DHAV‑1 which is epornitic in nature causes a major devastating disease endangering duck farming
Prevalence of urinary shedders and characterization of pathogenic Leptospira among cattle population in Tamil Nadu ‑ Implications for control
Get PDFBovine leptospirosis causes jaundice, mastitis, infertility, abortion, and death of infected animals. This research aimed to study the status of urinary shedders of pathogenic Leptospira among the cattle population and identify the infecting serogroup circulating in the state of Tamil Nadu (India). A total of 305 blood and 305 urine samples were collected from organized farms (n = 44), individually housed animals (n = 81) and animals from the slaughterhouse (n = 180). Microscopic agglutination test was carried out to detect anti‑leptospiral antibodies. Dark‑field microscopic examination and culture of urine were done to detect and isolate the Leptospira. The isolated Leptospira were identified by cross‑agglutination test and gene sequencing. PCR and real‑time PCR were carried out to detect leptospiral genomic DNA in urine samples to detect the shedders. The anti‑leptospiral antibodies were detected in 6.2% of animals. The Leptospira genomic DNA was detected in 9.2% (28/305) of urine samples. Of the 28 Leptospira positive urine samples, 39.2% were from animals with clinical signs suggestive of leptospirosis and 60.8% Leptospira positive samples were from slaughterhouse animals. The Leptospira isolated were identified as Leptospira interrogans serogroup Sejroe and Hebdomadis. The present study demonstrates the need to include leptospirosis in cattle health surveillance programmes to prevent leptospirosis and renal carriage by vaccination
Molecular characterization of Ornithobacterium rhinotracheale strains isolated from chickens in the Northwestern Iran
Get PDFOrnithobacterium rhinotracheale is the etiological agent of chickens and turkeys’ respiratory diseases, reduction of eggs, growth retardation, and death. Present research aimed to conduct the isolation, recognition, and molecular investigations of the bacterium in commercial broiler chicken flocks in East Azerbaijan province, Northwest of Iran, by the partial sequencing of 16S rRNA gene, ERIC‑PCR, and RAPD‑PCR with the OPG11 and M13 primers. We obtained 330 specimens from tracheal swabs of 33 slaughtered broiler flocks, of which we found 14 isolates (4.24%) of five flocks (15.15%) to be O. rhinotracheale. Typing by RAPD assay with the OPG11 primer, and ERIC‑PCR, classified the isolates in two types of 1 and 2 molecular patterns, most of which belonged to type 1. However, the M13 primer‑based RAPD technique was inappropriate for distinguishing and categorizing the isolates and generating all of them in the same pattern. In a phylogenetic analysis of O. rhinotracheale based on 16S rRNA sequences, the strains generated three clusters (I‑III), in which all of the studied isolates fell in one cluster (cluster I). Based on the results obtained from the RAPD and ERIC‑PCR assays, the genetic patterns of broiler‑chicken‑isolated O. rhinotracheale strains in Northwestern Iran had no significant differences
Study of fear and fear evoking stimuli in a population of domestic dogs in Iran: a questionnaire‑based study
Get PDFGeneral and social acceptance, condition, and rules for dog ownership in Iran are different from western countries. This phenomenon leads to dissimilar fear and fear evoking stimuli in dogs. Stress and fear-related factors and dog’s behavioral problems are not fully studied in Iran. Thus, the aim of the current study was to measuring fear-related factors in dogs in Iran. In the first part of this study, demographic information of the owners and dogs was collected using questionnaire from clinics and veterinary hospitals in Tehran. In the second part, behavioral tests were conducted. Questionnaire data were recorded based on 4 items as neuroticism, fear caused by other dogs, fear caused by human and separation behavior. The social contact test was used to determine dog’s experience for greeting, cooperation, and handling. According to the results, 69.3% of the dog owners were women and 30.7% men. 84.1% of the dogs were small breed and 15.9% large-breed dogs. 63.6% of the owner’s complained for impact of laws and prohibitions on their dogs' access to outdoor. The score for greeting, cooperation and handling were 1.76 ± 0.93, 3.06 ± 1.25 and 2.44 ± 1.21, respectively. Mean score for neuroticism, fear caused by other dogs, fear caused by human and separation behavior were 29.69 ± 9.24, 14.75 ± 5.38, 9.84 ± 4.05 and 6.57 ± 3.05, respectively. Principal component analysis (PCA) revealed that fear using neuroticism, fear caused by other dogs and human were 1st fear related factor while separation-related behavior was the 2nd priority (P = 0.001). Small-breed dogs had more separation behavior and fear caused by other dogs compared to the large-breed dogs (P = 0.001). These findings suggested keeping the dogs indoor with limit access to a yard had negative impact on dog’s behaviors. Legal restrictions have adverse effect on fear behavior in dogs. Determination of fear-related factors might prove useful for dog’s behavioral intervention in Iran
English
No full textLeishmaniasis is a widespread disease caused by species of the genus Leishmania. In Colombia, this zoonosis is endemic in rural areas with a high prevalence in the departments of Antioquia, Santander, Meta, Tolima and Nariño. Dogs are the most important domestic reservoirs of the pathogen, given the epidemiological importance of dogs in the control of leishmaniasis is needed to determine the prevalence of Leishmania spp. in canine population of the rural area of Ibagué and to identify potential risk factors related to the presence of this parasite. A cross-sectional study was carried out in 173 dogs from the rural area of Ibagué. Leishmania spp. was detected by amplifying the Internal Transcribed Spacer (ITS-1) and two regions of the hsp70 gene through PCR. Factor associations were calculated through the Chisquare and odds ratio. Prevalence of Leishmania spp. infection in dogs was of 91.33% (158/173), where 36.71% (58/158) of the Leishmania spp. positive dogs showed one or more clinical signs of canine leishmaniasis and 63.29% (100/158) of the dogs were asymptomatic. Factors associated with the presence of the parasite did not show significance. In addition, hsp70D-PCR was proved to be highly efficient for the detection of Leishmania spp
Examination of organs and tissues of adult sheep grazed in an area with possible intoxication with rocket fuel, Kazakhstan
Get PDFThe authors have conducted experiments to study the pathoanatomical and histological pattern of organs and tissues of adult sheep affected by unsymmetric dimethylhydrazine (UDMH). This highly toxic fuel was spilled on the territory of the Karsakpay and Ulytau districts of Karaganda region, Kazakhstan, because of the fall of the rocket ‘Proton‑M’ after an unsuccessful launch from the Baikonur cosmodrome in 2007. In the experiment, the study group was consisted of 7 adult sheep that grazed in the area of possible intoxication with rocket fuel UDMH. The main objects of the study were histological preparations obtained from fixed structures. As the structures have a flat contrast and are poorly detected in the ordinary light microscope, the specially processed preparations were used. After preparing, the authors studied organs and tissues using a microscope, which allowed to reveal in detail the level of damage caused by intoxication and to establish the negative effect of UDMH on the internal organs. The group of sheep showed a high index of macroscopic signs of interstitial pneumonia (85.7 ± 14.3%), and histologically quite high index was granulomatous inflammation of liver (71.4 ± 18.4%). Kidneys also showed a high level of abnormalities
Assessment of a new microsatellites panel for traceability in Italian inbreed pigs using parentage test
Get PDFThe origin of meat and meat products can be traced by verifying the identity of an offspring from its parents’ genotypes. Although there are many microsatellite panels applicable to swine population, efficiency of parental testing decreases when the population consists of consanguineous animals. The aims of the present study were to develop a new microsatellite panel for traceability using parentage test in inbreed pig population and to assess how hybridization can influence the efficiency of parental testing. A new genotyping assay, based on 20‑microsatellite assay, was performed in 304 individuals consisting of related and unrelated animals. The results showed that the microsatellites used in this study display high level of polymorphism ensuring a parentage assignment of 100%. This genotyping panel can be a useful tool to test a ’parent‑to‑fork’ traceability system based on 20 microsatellite loci and can overcome technical limitations in inbreed population