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    3261 research outputs found

    Cytocompatibility of 3T3 fibroblast and HUVECs

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    Representative live/dead staining shows high viability of cells seeded on the dECMMA scaffold, with live cells stained green and minimal dead cells (red), indicating high cytocompatibility.Confocal microscopy imaging of actin filaments (green) and nuclei (DAPI, blue) demonstrates robust cellular spreading, adhesion, and cytoskeletal organization.Immunofluorescent staining of CD31 illustrates HUVEC organization and formation of vasa vasorum-like structures (indicated with yellow arrows); nuclei stained with DAPI (blue).Quantitative metabolic activity assessed by Alamar Blue assay comparing human umbilical vein endothelial cells (HUVECs) and fibroblasts (3T3) proliferation within dECM scaffolds over time, confirming favorable cellular viability and metabolic activity</p

    Data for publication entitled: Silica-coated gold nanoshells. Surface chemistry, optical properties and stability

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    Research data that was used to create the publication entitled: Silica-coated gold nanoshells: Surface chemistry, optical properties and stability. Gordel-Wójcik Marta, Pietrzak Michał, Kołkowski Radosław, Zych Eugeniusz. Journal of Luminescence, 2024, 270, 120565/1-120565/9. DOI: 10.1016/j.jlumin.2024.120565This study provides a detailed investigation into the optical characteristics of colloidal gold nanoshells (NSs) with varying gold layer thicknesses and an additional silica coating. Our findings indicate that the spectral position of the localized plasmon resonance in NSs is highly sensitive to these parameters. Additionally, we observe that the silica layer plays a crucial role in enhancing the stability of colloidal systems. To complement our experimental results, we perform theoretical calculations that illustrate how absorption and scattering contribute to the overall extinction as a function of gold layer thickness. These calculations also shed light on the mechanisms behind spectral broadening in clustered NSs. Our results offer valuable insights for designing hybrid materials with applications in refractive-index sensing, metal-enhanced fluorescence (MEF), and surface-enhanced Raman scattering (SERS).Please consult the readme.txt file for additional information.</p

    Date fruit textural features under freezing storage

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    An innovative approach based on image analysis was used to assess the external quality of fresh and frozen ‘Mejhoul’ and ‘Boufeggous’ date palm cultivars stored for 6 months at −10 °C and −18 °C. Their quality was evaluated, in a non-destructive manner, based on texture features extracted from images acquired using a digital camera and flatbed scanner.The whole process of image processing was carried out using MATLAB R2024a and Q-MAZDA 23.10 software. The extracted features are reported in the four datasets based on &#34;image acquisition device&#34;, &#34;date cultivar&#34;, and &#34;Storage temperature&#34;.</p

    Automated sequential SPME adressing the displacement effect in food samples

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    The research focuses on developing an automated sequential solid-phase microextraction (SPME) strategy to address the displacement effect in food samples, particularly for analyzing volatile compounds in beer. The study introduces a two-step extraction process using different SPME coatings to first remove high-affinity nonpolar compounds before extracting polar analytes, which improves extraction efficiency. This method, integrated with GC–MS analysis, eliminates the need for manual intervention, making it more practical for automated food analysis. The results show enhanced detection of polar compounds while maintaining quantification accuracy for nonpolar ones.Data contains a ReadMe.txt file.</p

    The influence of HIV sense and antisense transcripts on stochastic HIV gene expression and reactivation

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    In this study, we established dozens of single provirus-infected cellular clones offering various transcriptional phenotypes of HIV. We proposed that stochastic fluctuations in HIV transcription can appear at, at least, two levels: (1) the chromosomal landscape and (2) the in situ HIV integration site. In the former case, proviruses integrating at different genomic locations demonstrated a variety of transcriptional bursting and can be classified in noise space constructed based on the parameters associated with the coefficient of variation and using a mathematical model fitting a curve of exponential decay. In the latter case, stochastic HIV transcription can be unveiled through its phenotypic bifurcation and tended to be a pure epigenetic phenomenon: the identical provirus demonstrated fluctuations in its transcription with an elevated frequency. We observed similar expression patterns between sense and antisense RNA transcripts. Notably, both HIV long terminal repeats reacted to drug stimulation and may reveal distinct behaviors. Overall, our data suggest that HIV antisense transcripts could be involved in the stochastic nature of HIV transcription.</p

    The profile of amino acids in serum and urine of women with overweight and obesity after 8-week energy-restricted ketogenic diet

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    The dataset contains raw data obtained in a randomized, single-centre, controlled trial was conducted with adult, non-menopausal women with a BMI of 25.5-35 without any chronic diseases, which were following energy-restricted ketogenic diet for eight weeks. Data were collected at the baseline, after 4 weeks, after 8 weeks of the nutritional intervention.The study protocol details can be found here: https://doi.org/10.1371/journal.pone.0285283The study has been registered in ClinicalTrail.gov. Registration number: NCT05652972.Please consult the readme.txt file for additional information.</p

    TLS scans and BIM model of some Rzeszow University of Technology buildings

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    TLS scans and BIM model of some Rzeszow University of Technology buildings for testing the accuracy of terrestrial laser scanning and creating reverse BIM models of existing buildings.File 1 contains raw Faro scans. Scans can be accessed by commercial and open source software (e.g. Cloud Compare).File 2 and 3 contains Autodesk Revit 3D model and it&#39;s IFC export and can be accesed by Autodesk Revit or wieved by Autodesk Viewer a free Autodesk software or other BIM software.</p

    Bactericidal activity of selected inhA inhibitors - raw data

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    The aim of the research was to identify new inhibitors of mycobacterial cell wall biosynthesis that inactivate the FAS2 system through interaction with enoyl acyl carrier protein reductase (InhA).Raw data of biological part have been deposited including: (i) Assessment of minimum inhibitory concentration (MIC) for the growth of tuberculosis bacilli and Mycobacterium abscessus obtained using the MABA test (microplate alamar blue assay). Initial screening of antibacterial activity was conducted at a concentration of 125 μg/mL, and then MIC values were determined for compounds showing activity (numerical data in Excel, JPEG images of plates); (ii) Determination of bactericidal concentration of selected compounds against M. tuberculosis performed using colony forming unit (CFU) count assessment, comparing the number of viable bacterial cells before compound addition, after 168 and 336 hours of incubation in the presence of test compounds. Data are included in an Excel table. (iii) Assessment of cytotoxicity of test compounds against murine fibroblast cell line L929. Data are included in Excel format. (iv) Evaluation of the effectiveness of test compounds against tuberculosis bacilli forming biofilms. Raw data obtained and statistical significance analyses (Ordinary One-way ANOVA) are included in an Excel file. (v) Assessment of the activity of test compounds against tuberculosis bacilli located within human macrophages, human monocyte-derived macrophages (MDMs), was performed after 48-hour incubation in the presence of test compounds by plating on solid media (CFU). The obtained data and their statistical analysis (Ordinary One-way ANOVA) are presented in an Excel file.</p

    The fluctuation-driven dielectric properties of liquid crystalline 8OCB and its nanocolloids

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    The results of broadband dielectric spectroscopy (BDS) studies 4&#39;-(Octyloxy)-4-biphenylcarbonitrile (8OCB, CAS no: 52364-73-5) liquid crystal and its colloids doped with paraelectric BaTiO3 nanoparticles (diameter d &#61; 50 nm, the cubic crystal system, nanoparticles concentrations: 0.4, 0.5, 1 wt%). The data was collected on cooling from 373.2 K to 273.2 K. Complex dielectric permittivity measurements were performed using two analyzers: Novocontrol Alpha-A analyzer in the frequency range from 1 to 10MHz and Keisight analyzer in the range from 1 MHz to 3 GHz. The results include frequency changes in the real (ε’( f )) and the imaginary (ε’’( f )) part of the complex dielectric permittivity, temperature changes of dielectric constant, loss curve maximum, relaxation time distribution parameters, and relaxation times.The names of the individual files correspond to the numbering of the figures in the paper: Łoś, Joanna, et al. &#34;The fluctuation-driven dielectric properties of liquid crystalline 8OCB and its nanocolloids.&#34; Soft Matter 18.24 (2022): 4502-4512. DOI: https://doi.org/10.1039/D2SM00105EFiles included in this collection:Fig01 – dielectric spectra of 8OCB and colloids with 0.5% and 1% addition of BaTiO3 measured at 360 K, 345 K, and 330 K.Fig02_03 – the dielectric constant as a function of temperature.Fig04 – the loss curve maximum as a function of temperature.Fig05 – m and n parameters, describing relaxation time distribution, as a function of temperature.Fig06 – relaxation time as a function of reciprocal temperature.Fig07_08 – DC conductivity as a function of relaxation time.Fig09 – dielectric spectra measured in 8OCB&#43;0.5%BaTiO3 sample at 370 K, 353 K, and 330 K.</p

    Raw data for the original article: D. Bury et al. "Photocatalytic MXene-Magnetite Capsules for Industrial Dye Wastewater Treatment"

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    Załączone dane prezentują:Pliki:&#34;Adsorbcja po 3 min - Dane z absorbancji światła z czytnika płytek&#34;&#34;Adsorbcja po 6 min - Dane z absorbancji światła z czytnika płytek&#34;&#34;Adsorbcja po 15 min - Dane z absorbancji światła z czytnika płytek&#34;zapisane w formacie xlsx, stanowią zbiór wyników procesu adsorpcji przeprowadzonego z enkapsulowanymi materiałami 2D - węglikami tytanu Ti3C2 oraz węglikami niobu Nb2C tzw. MXenes wraz z magnetytem. Wynik zostały uzyskane z pomiarów prezentujących zmiany absorbancji światła przez próbkę, wskazującą na stopień oczyszczenia barwników modelowych oraz przemysłowych. Pomiary wykonywano przy użyciu czytnika płytek.2. Pozostałe pliki stanowią zbiór danych z charakteryzacji materiałów MXenes, magnetytu i barwników, obejmujący:Właściwości optyczne (spektrofotometria UV-Vis): Analiza ta skupia się na właściwościach optycznych barwników. Analiza opiera się na absorpcji energii przez elektrony w cząsteczkach, prowadząc do przejść elektronowych – głównie z orbitalu podstawowego (np. HOMO) na orbital wzbudzony (np. LUMO). Spektroskopia UV-Vis jest powszechnie stosowana do badania stężenia i degradacji barwników organicznych w roztworach wodnych.Charakteryzacja wiązań - spektroskopia FTIR (Fourier Transform Infrared Spectroscopy): to technika analityczna służąca do identyfikacji związków chemicznych oraz analizy struktury cząsteczkowej materiałów na podstawie ich charakterystycznych drgań molekularnych. Pomiar polega na rejestrowaniu widma pochłaniania promieniowania podczerwonego przez próbkę, co pozwala określić obecność określonych grup funkcyjnych. Metoda ta jest szeroko stosowana w inżynierii materiałowej do badania polimerów, kompozytów, nanomateriałów, cienkich warstw czy produktów degradacji chemicznej. Analiza FTIR pozwala m.in. na ocenę czystości materiału, identyfikację produktów reakcji chemicznych oraz monitorowanie zmian zachodzących w strukturze powierzchni w wyniku modyfikacji lub starzenia się próbki.Czystość materiału (XRF - fluorescencja rentgenowska): Technika ta została użyta do określenia czystości materiału MXenes poprzez analizę jego składu pierwiastkowego. XRF to metoda nieniszcząca, która pozwala na dokładne określenie, jakie pierwiastki znajdują się w próbcePliki graficzne w formacie .tif &#34;Kapsulki_ALG_MAG_MXene&#34; (_1, _2 i _3) zawiera zdjęcia ze skaningowego mikroskopu elektronowego zrobione dla kapsułek zawierających różne stężenia poszczególnych elementów kompozytów, z których składają się kapsułki. Zdjęcia SEM umożliwiają dokładną obserwację morfologii i struktur kapsułek, w tym ich powierzchni, wielkości oraz jednorodności materiału. Obrazy te dostarczają cennych informacji na temat interakcji między poszczególnymi komponentami materiału w kontekście ich zastosowania w procesach fotokatalitycznych.Załączone pliki stanowią kompleksową analizę syntezy, charakteryzacji i wydajności materiałów fotokatalitycznych opartych na MXenes, szczególnie w kontekście zmian absorbancji światła oraz czystości materiału. Zdjęcia SEM pozwalają na wizualizację struktur kapsułek, a pozostałe dane pomagają w zrozumieniu ich właściwości optycznych oraz skuteczności w procesie oczyszczania barwników. Całość stanowi ważny krok w kierunku opracowywania bardziej efektywnych, bezpiecznych i trwałych materiałów, które mogą odegrać istotną rolę w zrównoważonym rozwoju technologii ochrony środowiska.</p

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