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A Comprehensive Review on Cytoskeletal Organization and Its Interaction with Polysaccharides and Enzymes during Primary Cell Wall Development
No full textA well-coordinated process is required to construct a complicated structure like the cell wall, which consists of several elements that must be joined appropriately from various sources inside the cell. In order to successfully moderate dynamic responses to developmental and environmental signals, further complexity is necessary. The plasma membrane is continually and actively transporting sugars, enzymes, and other cell wall elements throughout diffused development. Actin filaments and microtubules make up the cytoskeletal pathways used to transport cell wall elements in vesicles during cell division. In addition to these elements, other proteins, vesicles and lipids are transported from and to the cell plate while cytokinesis occurs. Adding additional cell wall material or building a new cell wall requires a rearrangement of the cytoskeleton, which we examine in this review first. We next look at the commonalities between these two processes. Our next topic is the transport of cell wall-building polysaccharides and enzymes via motor proteins and other interactions with the cytoskeleton. Final thoughts on cytokinesis-generated cell walls include a look at some of their unique properties
Production of Fungal Pectin Lyase and Polygalacturonase from Fruit Wastes by Solid State Fermentation
No full textPolygalacturonase (PG or PGase) and Pectin lyase (PL) are depolymerase enzymes that split the α-1, 4-glycosidic linkages in the backbone of homogalacturonans. They are produced by microorganisms degrading pectin-containing substrates. PG and PL were produced in the solid-state fermentation (SSF) of beans testa (BT), mango peels (MP), Plantain peels (PP), and BT: MP: PP (1:1:1). Substrates were seeded with individual fungal strains namely Aspergillus tamarii, Aspergillus terreus, Aspergillus piperis, Aspergillus parasiticus and Mucor piriformis. PG production ranged from 0.0377 - 141.0095 U/g with A. tamarii, A. terreus and A. piperis producing the highest on mango peels. PL production ranged from 50.50 - 10,852.50 U/g with Mucor piriformis producing the highest on plantain peels. The pH of the fermentation medium changed during growth, metabolism, and pectinase production. The best pH for pectinase production falls within the acidic range. Unconventional substrates such as PP are viable for pectinase production, and PL yield in SSF is improved by compositing substrates
Serratia nevei 9rpt1, a Potential Microorganism for Phosphorus Recovery
No full textAims: Phosphorus (P) is an essential element for life, finite and irreplaceable. Its constant exploration on a global scale has motivated frequent alerts regarding an eventual crisis due to the shortage of this nutrient. However, it is possible to recycle it and reintroduce it into the ecological cycle. One viable alternative is the microbial recovery of phosphate.
Study design: This study is based on systematic bioprospection of bacteria in phosphate-deficient Amazon regions.
Place and Duration of Study: Bacteria were isolated from black water samples, collected in the Rio Pretinho, located at Serra do Aracá, Barcelos, Amazonas, Brazil, from January to July 2019.
Methodology: Microbial isolation was performed in Luria Bertani agar medium. For the genomic study, the isolate with the best performance in the phosphate uptake test was chosen. The WGS was carried out in a Illumina HiSeq 2500 System. The assembly of the draft genome was carried out with the SPAdes and the annotation by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP).
Results: Serratia nevei 9rpt1 recovers 90% of the phosphate available in the culture medium. Its draft genome comprises 5.4 MB, the GC content is 59.52% and 4,922 coding sequences were identified, among these, two pst operons: one complete, containing the five pst genes and one missing pstS, pstC and phoU genes.
Conclusion: Serratia nevei 9rpt1, isolated from an Amazonian environment poor in phosphate, is very efficient to uptake this nutrient in a Pi starvation condition. The genomic findings revealed this strain has an additional high affinity Pi uptake pst system containing the ATP-binding protein PstB, the canonical permease PstA, a putative permease other than PstC, upstream of the PstA and two essential enzymes in the polyphosphate metabolism: polyphosphate kinase 1 and exopolyphosphatase
Micromorphological Study of Plant Fragments in Some Powdered Medicinal Plants Commercially Sold in Enugu Nigeria
No full textPowdered samples of 10 medicinal plant species purchased from different herbal medicine sellers in markets across Enugu metropolis and Iwollo market in Ezeagu Local Government Area Enugu state Nigeria were studied anatomically in search of micomorphological characters to identify the original plants used in the preparation. Moistened head of the needle was used to transfer samples onto a labeled glass slide containing 1 - 2 drops of water and plant stains; covered with cover slip and warmed gently to remove air bubbles. Samples were observed under the microscope in search of intact whole tissues and cells which could be used to identify the species of plant. The main characteristics of the fragments recovered from the samples are, parenchyma cells, trichomes, sclereid tissue system and long, branching non-septate fibre as in E. sonchifolia, T. terrestis, P. santalinoides; stomata as in C. odorata; elaioplast/oil storing cells as in C. pallida and H. surattensis; and then sheath cells as in U. chamae. Two of the plant samples studied did not display structures clear enough to identify them; while one of the samples had some structures recovered but wasn’t helpful enough to identify the plant. Thus, the study suggested that an examination by microscopy can provide a form of identification of plants from processed plant materials
Hepatoprotective Activity of Aqueous Extract of Cashew Apple Cake (Anacardium occidentale L.) in Rats and Mice
No full textAims: The aqueous extract of cashew apple cakes, rich in molecules of pharmacological interest, could be used against many diseases. Thus, the aim of the present work is to evaluate its hepatoprotective activity in rats and mice.
Methodology: Two batches of rats weighing between 200 and 300 grams, with five rats per batch, were pretreated for eleven days with the aqueous extract of cashew apple cakes at concentrations ranging from 150 to 300 mg.Kg-1 bw, then intoxicated with paracetamol at 2 g.Kg-1 bw, for three days. The blood of these rats was collected and submitted to biochemical analyses.
Two batches of mice weighing between 20 and 30 grams, with five mice per batch, were pretreated with the same test substance at concentrations also ranging from 150 to 300 mg.Kg-1 bw, then intoxicated with paracetamol before receiving phenobarbital. Afterwards, their sleep time was evaluated.
Results: In rats, paracetamol intoxication materialized by the increase in serum ALT activity ranging from 109±5.19 to 571±20.28 IU.L-1 and that of AST ranging from 144±5.77 to 428±14.19 IU.L-1. Similarly, direct bilirubin increased from 0 to 1.08± 0.58 mg.dl-1. These increases in transaminase activity and bilirubin levels were significantly decreased in rats pretreated with the aqueous extract of cashew apple cake. In mice, the phenobarbital test showed a 29.82 to 38.59% decrease in sleep time in mice pretreated with the aqueous extract of cashew apple cakes.
Conclusion: The aqueous extract of cashew apple cakes influencing biochemical parameters such as ALT, AST, bilirubin and sleep time, could therefore be used in the prevention of liver diseases, in traditional medicine. 
Physico-chemical Characterization and Polyphenolic Composition of Red Wines Produced from Autochthonous Grapes Varieties (Vitis vinifera) in Turkey
No full textAims: Local varieties of “Hönüsü” and “Horozkarası” red grapes have been evaluated for the production of red wine and characterized33 for their chemical and sensory characteristics.
Study Design: This research was initiated by the Food microbiology researcher and Applications Unit of Fermentation Laboratory.
Place and Duration of Study: Laboratory of Fermentation of the Food Engineering Department in Gaziantep University, October 2019 to May 2021.
Methodology: All wines were produced by a standard procedure of vinification. Three types of red wines were produced from combinations of red grapes. Ten phenolics were quantitatively detected in the red wines during processing. Brix, alcohol, pH and free SO2 contents were also detected.
Results: The results showed that the type of technology affects significantly (p<0.05) the level of phenolic compounds formed during processing. Horozkarası” red grape contributed to the highest amount of phenolic compounds in red wines. Gaziantep wine provides valuable information about the production of red wine from Gaziantep red grapes.
Conclusion: The phenolic compounds of red wines were significantly (p<0.05) higher than that of musts. Many of the remarkable features of the phenolic profiles and Brix of grape varieties could help us to characterize Gaziantep wines. The mixture of must from “Hönüsü” and “Horozkarası” red grapes with 7:3 ratio contributes suitable sugar and phenolic compounds for red wine. The results from this study provide valuable information about the red wine produced from the ancient grape varieties of the Southeast region
Feasibility of Building a Biotech Industry
No full textoai:ojs.pkp.sfu.ca:article/1The performance of Cuban biotechnology from 2008 to 2021 has shown a growing pipeline of valuable biomedical solutions. As the transformation of this sector into an industrial group has changed its R&D productivity and bio manufacturing capacity, we summarize here the results of R&D projects with impact on public health, constraints found in Cuba, lessons, and opportunities to develop this science-based industry in developing countries
Molecular Identification of Bacillus Species during Spontaneous Fermentation of Lima Bean Flour (Phaseolus lunatus)
No full textAims: Bacillus species is used as starter culture to improve quality of the fermented product. Thus, the purpose of this study is to identify Bacillus species during the spontaneous fermentation of Phaseolus lunatus with prospective selection as starter cultures.
Study Design: Spontaneous fermentation of Phaseolus lunatus flour was allowed to proceed at ambient temperature (29±2°C) for three days under anaerobic condition.
Methodology: The Bacillus counts were determined and 100 isolates were identified by PCR and the sequencing of 16S rDNA domain.
Results: In unfermented sample the Bacillus count was 3.14 log CFU/mL. During fermentation the count being between 2.68 and 2.88 log CFU/mL. Based on PCR and the sequencing of 16S rDNA domain, Bacillus isolates were assigned to four species Bacillus sp, Bacillus subtilis, Brevibacillus agri and Bacillus xiamensisis. Bacillus spp, Bacillus subtillis and Bacillus cereus were detected at all the fermentation times. Their frequencies were between 14.29 and 45.83%, 25 and 35.71%, 25 and 50% respectively.
Conclusion: Among these species Bacillus subtillis could be used as starter culture to improve quality of the fermented product
Ameliorative Effect of Vitamin E on Paraquat Induced Haematological Disorder in Male Albino Rats
No full textParaquat is a controversial herbicide that can increase reactive oxygen species levels by undergoing redox cycling and producing reactive oxygen species such superoxide anion. Vitamin E is a fat soluble vitamin that modulates oxidation processes in the body due to its particular antioxidant activity. It is a powerful chain-breaking antioxidant that limits the synthesis of reactive oxygen species molecules. The goal of the study was to see if vitamin E had a short-term therapeutic impact on paraquat-induced male albino rats. For the experiment, 200 male albino rats were employed. The 200 rats were separated into four primary groups (A, B, C, and D), each of which included 50 rats and was then subdivided into two subgroups, each with 25 rats. The "A" group was not induced paraquat, but the "B," "C," and "D" groups were induced 0.02g, 0.04g, and 0.06g of paraquat, respectively. The "A" group was divided into two subgroups: "A0" and "AVE," which represented the subgroups that were not given Vit E and those who were given Vit E (500 mg) respectively. This design was similar for groups "B," "C," and "D." The frequency of paraquat introduction was fortnightly for three months, then followed by vitamin E treatment weekly for two month. After the treatment with Vit E, the rats were sacrificed and blood was taken to analyze several hematological parameters (Hemoglobin concentration [Hb], Packed Cell volume [PCV], Total White blood cell count [T-WBC], Neutrophils and Lymphocytes). These hematological parameters differed significantly across the "Ao," "Bo," "Co," and "Do" groups, at p-value < 0.05. This was equally true for the "Ave," "Bve," "Cve," and "Dve," groups at p-value < 0.05. The findings also revealed that intra-group comparisons of Hb, PCV levels between B0 vs Bve, C0 vs Cve, and D0 vs Dve were significant different, p-value<0.05 whereas WBC, neutrophils and lymphocytes were not p-value>0.05. This study has shown that PQ insult on anaemia indicators in rats can be corrected by a weekly administration of the vitamin
Monkeypox and Monkey Fever: Basic Understanding for Better Community Participation in Disease Control
No full textCurrently, Monkeypox is a threatening viral disease terminology around the world that worries the global community after the Coronavirus pandemic. Beyond the woods of central Africa, where cases were first discovered, the geographic distribution of monkeypox cases has extended to other parts of the world including India, where cases have been imported. Under these circumstances, public confusion is noticed between Monkeypox and Monkey fever across southern parts of India. Monkeypox and Monkey fever (Kyasanur Forest Disease) are categorized as potential emerging or re-emerging Zoonotic diseases of humans and are fundamentally having clear-cut differentiation in their causative agent, clinical symptoms, and pathogenesis. Hence, it is important to clarify and eliminate the confusions which would have tremendous and dangerous public health impacts. As defined by the Global Burden of Disease Study, Emerging Infections (EIs) are those that have recently appeared in a population or previously existed but are now gaining in prevalence or geographic spread rapidly. A disease emerges from the interactions between rapidly evolving infectious agents and the environmental changes and changes in human behavior that provide these agents with favorable ecological niches. This review attempts to differentiate between the two emerging infections - Monkeypox and Monkey fever which are affecting a significant group of the population worldwide and provides a comparative picture of both diseases towards developing adequate awareness among the public and expects to complement the community-based future control measures