World Journal of Experimental Biosciences
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Outbreak of Echoviruses in middle and south Iraqi provinces
Get PDFoai:ojs2.journals.uniscipub.com:article/4This study included 210 patients with Enteroviral symptoms. All samples were collected from middle and south Iraqi provinces. The highest positive percentage was found in Baghdad (28.8 %). The percentages in the other provinces were 26.7 %, 20 % 20 % and 10 % in Basrah, maysan, Karbala and Wasat, respectively. The lowest percentage was reported in Thy-Kar (8 %). It was found that Echovirus type 11 responsible for 50 % (20 case infected with this type of virus) of infection with Echovirus and this percentage was declined for the following virus, Echovirus 1 (20 %), Echovirus 3 (17.5%), Echovirus 22 (7.7%) and Echovirus 5 (5 %)
Plaque Forming Cell Technique for Counting IgG and IgM antibody-secreting cells in peripheral blood of patients with rheumatoid arthritis
Get PDFAntibody Secreting cells are playing a central role in pathogenesis of several autoimmune diseases especially in rheumatoid arthritis. There are few reports describe a method can estimate the number of antibody secreting cells in vitro spontaneously. Here were modified the classical method of plaque forming assay to study the number of IgG and IgM secreting cells [Plaque forming cells (PFCs)] without external stimulators in vitro in peripheral blood of patients with rheumatoid arthritis. Moreover, the relationship between the IgG and IgM concentrations and number of PFC-IgG and IgM (without external stimulators) was studied. The significant increase of IgG as well as IgM antibody secreting cells [plaque forming cell (PFC) - IgG and IgM) was seen in peripheral blood of patients with rheumatoid arthritis (P<0.001) as compared with healthy control group. These findings were associated with high levels of IgG and IgM concentration in peripheral blood of patients. The current present study besides providing a modified method to detect IgG or IgM secreting cells (PFC) in vitro, it also proved that the high level of IgG and IgM in peripheral blood is associated with spontaneous activity of B-cells.
 
T-Lymphocytes Subsets in inactive carrier state of HBV
Get PDFThis study included nine patients with inactive carrier states of HBV and 14 healthy control groups. The number and the percentage of T-lymphocyte (CD3+ Cells) in the peripheral blood of these groups showed no significant difference. Similar trend was observed when number and percentages of T helper cells (CD4+ cells) and T cytotoxic lymphocytes (CD8+ cells). Moreover, no significant difference in CD4+ /CD8+ cells ratio (P > 0.05) in peripheral blood of patients with inactive carrier state of HBV as compared with healthy control group. The levels of total serum bilirubin (TSB) concentration and alanine aminotransferase (ALT) activity were similar to control group. The levels of immunoglobulin concentration (IgG and IgM) in patients group were similar to control group. No remarks of autoimmune phenomena were observed in patients group.
 
Thermoregulation of IL-1α production and phagocytic activity of Escherichia coli Lipopolysaccharide-induced mononuclear cells
Get PDFPeripheral blood mononuclear cells were stimulated with lipopolysaccharide and incubated at different temperatures (34, 37 and 39 oC). Interleukin-1 alpha (IL-1α) was measured. Phagocytic activity of LPS-stimulated monocytes in terms of bacterial uptake and intracellular bacterial killing was checked at different temperatures and time intervals in vitro. Early elevation IL-1α was found in LPS-stimulated monocytes that incubated at 39 oC followed by cells that incubated at 37 oC and lowest level was detected at 34 oC. Similar trend was reported in the phagocytic activity in terms of bacterial uptake and intracellular bacterial killing. The sharp decrease in IL-1α was observed 12 h post exposure to LPS in LPS-stimulated monocytes that incubated at 39 oC only. While, the decrease of IL-1α levels in other incubated temperatures (34 and 37 oC) was seen later than incubated at 39 oC. This report describes the striking effect of incubation temperature on activity of LPS-stimulated monocytes. This result explains clearly the important role of elevation temperature in modulating the immune response against external pathogens
Isolation and Identification of Sabin poliovirus in middle and southern Iraqi provinces
Get PDFThree hundred and sixty feces samples were collected from children and infants who have diarrhea and other symptoms associated with Acute Flaccid Paralysis (AFP). The samples were collected from the middle and southern provinces of Iraq. The clinical cases were equal or less than four years old. Nineteen positive poliovirus positive cases were found post-preliminary culture. Poliovirus type I was found in six cases, Poliovirus type II was found in three cases and Poliovirus type III was found in six cases. Four cases contain mix poliovirus types. All positive cases were screened in confirmation test and the results showed that 9 isolates of type I, 3 isolates of type II and 10 isolates of type III. All isolated viruses were sabin-type type and no wild-type poliovirus was identified in current study. It can be concluded that the poliovirus sabin maybe associated with Acute Flaccid Paralysis