eKhSACIR інституційному репозитарії Харківської державної академії культури
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    7410 research outputs found

    Planification communale, concertation locale et préservation des espaces naturels et agricoles dans deux départements méditerranéens en France

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    Séance 2 - La reterritorialisation de l’agricultureInternational audienc

    Small non coding RNA from frozen bull sperm cells: A Biomarkers panel of male fertility

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    International audienceNew sperm function in embryo development have emerged recently, relying on their small noncoding RNAs (sncRNAs) content. Indeed, involvement of sperm-borne miRNAs in mouse epigenetic inheritance has been evidenced and paternal sncRNAs have been shown to be dispensable for fertilization but crucial for the development of zygotes and 2 cells-embryos. Our study was conducted to unravel the sncRNA content from bull frozen sperm cells and identify miRNA associated with fertility. Total RNA was extracted from 40 ejaculates originating from Holstein and Montbeliard bulls with contrasting fertility, using a novel enhanced protocol. The two quality controls were done on RNA to measure RNAs quantities (Qubit technology) and check whether a reference miRNA (miR125) could be detected by RTqPCR. NGS sequencing libraries were prepared using small RNA (<200 nucleotides) and sequenced at modest depth (40 million 50 bp single reads, Illumina HiSeq; Exiqon). Two thirds of the reads could be annotated as miRNA (16%), rRNA (13%), tRNA (7.5%), long non coding RNA (7.2%), as mitochondrial RNA (6.5%) and mRNA (17%). The remaining unknown sequences are consistent in terms of size with piRNA, which are known to be expressed spermatocytes, spermatids and sperm cells. By the use of miRDeep2 software, 3196 miRNA expressed in all samples have been identified, including 583 known and 2613 putative miRNAs. Statistical analysis carried out using the DESeq2 package, emphasized 47 differentially expressed miRNA between fertility groups. This study highlights the potential of sperm cells’ sncRNA as biomarkers for bull fertility

    Nouvelles controverses du développement territorial : quelle gouvernance et quelles innovations ?

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    Événement(s) lié(s) : - 15. Conference ERSA-GR; Athènes (GRC) - (2017-07-05 - 2017-07-07)Nouvelles controverses du développement territorial : quelle gouvernance et quelles innovations ?. 54. Colloque ASRDLF. 15. Conference ERSA-G

    Diversité des autonomies fourragères et alimentaires en exploitations agricoles laitières de polyculture-élevage du Pays de Caux et lien avec le degré d’intégration culture-élevage : approche exploratoire

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    Diversité des autonomies fourragères et alimentaires en exploitations agricoles laitières de polyculture-élevage du Pays de Caux et lien avec le degré d’intégration culture-élevage : approche exploratoire. Colloque Les polycultures-élevages, Valoriser leurs atouts pour la transition écologiqu

    The validation of analytical methods as an evaluation tool of research results reliability

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    The validation of analytical methods as an evaluation tool of research results reliability. Functional Ecology Conference AnaEE Franc

    Analysis of short food supply chains governance: innovative collective platforms supplying local produces

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    This paper addresses the issue of governance diversity of innovative semi wholesale SFSC such as multi stake-holder platforms acting as middlemen between local producers and buyers (individual and collective ones) wishing to procure local products. We apply the new institutional economics approach with special attention to ownership rights allocation (Hansmann, 1988), collective decision-making (Pozzobon and Zylbersztajn, 2013) and associated transaction costs (Williamson, 1991; Menard, 2004; Chaddad, 2012). Those concepts were already used in analysis of various agri-businesses, cooperatives and their vertical relations in supply chains but little has been done in terms of SFSC. We rely on this framework to provide a comparative analysis of their governance structures based on a detailed description of the “horizontal” relations among stake-holders and “vertical” relations between the platforms and their suppliers and customers

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    eKhSACIR інституційному репозитарії Харківської державної академії культури
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