Czasopisma Instytutu Hodowli i Aklimatyzacji Roślin
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Prowadzenie regeneracji zasobów genowych traw w trzyleciu w ramach Programu Wieloletniego 1.2 MRiRW, IHAR — PIB w ZDOO w Lisewie
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SAMPLING, MAINTENANCE AND PATHOTYPE IDENTIFICATION OF SYNCHYTRIUM ENDOBIOTICUM (SCHILB.) PERC.
Get PDFPlant Breeding and Acclimatization Institute – National Research Institute (IHAR-PIB) is responsible for pathotype identification of Synchytrium endobioticum isolates collecting from Poland. Pathogenicity tests are carried out using the Glynne-Lemmerzahl method, according to EPPO Standard PM 7/28. Pathotypes are defined based on their reaction to a range of well-characterized differential cultivars of potato. Assessment of one isolate of the fungus requires more than 20 differential cultivars. All pathotypes of S. endobioticum from Polish reference collection are multiplied and maintained (fresh warts) on tubers of extremely susceptible cultivars continuously. For all references pathotypes and isolates of S. endobioticum, the compost with winter sporangia are prepared for long-term of maintenance
ASSESSMENT OF POTATO RESISTANCE TO SYNCHYTRIUM ENDOBIOTICUM
Get PDFIn Poland the Plant Breeding and Acclimatization Institute - National Research Institute (IHAR-PIB) is responsible for officially assessing the resistance of potato breeding lines and cultivars to Synchytrium endobi-oticum. In the official assessment of wart resistance the modified Glynne-Lemmerzahl method is used. A full cycle of assessment of the wart disease resistance requires 42 - 45 tubers per cultivar/breeding line. Forty two tubers are used in laboratory tests. To complete the laboratory tests, another 10 tubers are inoculated with winter sporangia of the fungus, using ring test. The final results are available after 3 years of investigation. If necessary, the full cycle of resistance tests to S. endobioticum can be performed during one year on 15 tubers in each of the 3 replications (45 tubers in total).Molecular verification of potato genotypes resistance to pathotype 1(D1) is possible with the use of SCAR marker Nl25-1400. Nevertheless, an official phenotypical assessment of advanced breeding lines, as the final verification of their resistance, is required
PHYTOPHTHORA INFESTANS: ISOLATION OF PURE CULTURES, STORAGE AND INOCULUM PREPARATION
Get PDFPhytophthora infestans causes potato and tomato late blight, economically the most important disease of these plant species. The Oomycete pathogen is frequently sampled, isolated to pure cultures, stored, and char-acterized. The knowledge of its diversity, migrations and evolution is essential for breeding resistant plants and for designing appropriate control strategies. The article presents methods for collection, storage and prep-aration of P. infestans isolates for inoculation of plant tissues, based on the publication by Zarzycka (2001), later updated and modified
IN VITRO PRESERVATION OF POTATO PLANTS
No full textPotato plants free of viruses, bacteria and viroid could be maintained in vitro for a long time. Proper prepa-ration of potato plants for in vitro culture provide its long-term storage in good condition. First step is to es-tablish in vitro culture from young greenhouse grown plants in early developmental stage. Explants are main-tained and propagated by nodal subculture on hormone-free Murashige and Skoog (MS) medium at tempera-ture 20-22°C. After rooting, for longer preservation plants are maintained at temperature 8-10°C
VIRUS ELIMINATION FROM IN VITRO POTATO PLANTS
Get PDFThe routine and the most effective method of viruses elimination from potato plants is in vitro culture of apical shoots combined with thermo- and/or chemotherapy. At times electrotherapy or cryotherapy is also applied. Elimination of potato viruses by thermotherapy required treatment of infected plants with high tem-perature (35°C - 45°C) for about two weeks. Thermotherapy is useful for elimination of PLRV and PVY from potato plants. In chemotherapy antimetabolite, like ribavirin is used. Chemotherapy is dedicated for elimina-tion of PVM, PVS and PVX. Very often the successful virus elimination require a few cycles of thermo- and/or chemotherapy
EVALUATION OF TENDENCY TO BLACKSPOT BRUISING AND ENZYMATIC DISCOLORATION OF POTATO TUBERS
Get PDFEnzymatic discoloration of potato tubers initiated by mechanical impact bruising and the discoloration which appears after tuber cutting are crucial quality traits of the cultivated potato. The presented determina-tion of blackspot bruise susceptibility includes evaluation of the trait with two impact methods – the rotating drum and the falling bolt. These two methods affect both the physical and the biochemical properties of tu-bers. Evaluation of the discoloration potential after tuber cutting (ED) is also discussed. Evaluating the discol-oration potential of tubers is one of the most important points in determining potato quality
METHODS TO STUDY THE PVY POPULATION IN THE POTATO
Get PDFThe PVY population in the potato has been studied continuously using tobacco bait plants in potato fields at Młochów since 1980 at two-year intervals and in potato tuber samples collected from different regions of Poland since 2001 yearly. The paper presents the combined biological, serological and molecular assays for PVY identification and strain classification. Biologically, PVY strains are defined with respect to their ability to elicit hypersensitive resistance (HR) mediated by N genes in differential potato cultivars (King Edward, Desiree and Pentland Ivory) and to symptoms in the tobacco (cultivar Samsun). Serologically, an ELISA assay based on polyclonal or monoclonal cocktail antibodies recognizes all PVY strain types, while the specif-ic monoclonal antibodies help to recognize PVYN or PVYO/PVYC strains. Multiplex RT-PCR, Real-time RT-qPCR and sequencing-based assays are used to define the PVY genome structure. In the Polish population of PVY, the strains PVYO, PVYNTN, PVYN-Wi, PVYZ-NTN and PVYE were identified, while the PVYC strain was not detected
ISOLATION, IDENTIFICATION AND PRESERVATION OF PECTINOLYTIC BACTERIA PATHOGENIC TO POTATO
Get PDFBlackleg of potato plants and soft rot of tubers are caused by several species of pectinolytic bacte-ria from genera Pectobacterium and Dickeya. The text describes simple methods of isolating bacteria from symptomatic and symptomless organs of potato plants, their identification using Polymerase Chain Reaction (PCR) and preservation