Czasopisma Instytutu Hodowli i Aklimatyzacji Roślin
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Techniki metabolomiczne, oparte na LC-MS i NMR, w badaniach zmienności i różnorodności fitochemicznej roślin — na przykładzie roślin leczniczych
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Ocena reakcji odmian uprawnych i gatunków rodzaju Nicotiana na Tobacco mosaic virus oraz detekcja genu N warunkującego odporność typu nadwrażliwości
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Ocena zmienności i selekcja dawców fizjologicznej efektywności i tolerancji stresu w krajowej kolekcji grochu
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Aspekty prawne zachowania i stosowania roślinnych zasobów genetycznych w hodowli roślin i nasiennictwie
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Wykorzystanie zasobów genowych roślin ogrodniczych w badaniach nad kiełkowaniem, spoczynkiem i starzeniem się nasion
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Wykorzystanie allela S Capsicum spp. w badaniach genetycznych i w tworzeniu oryginalnego surowca przetwórczego
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COLORIMETRIC VS. CHROMATOGRAPHIC ANALYSES OF ALKALOIDS IN LUPIN SEEDS
Get PDFA characteristic trait of lupins is a production of alkaloids, which are a toxic and bitter taste compound ofseeds. Due to the lack of fast, sensitive and inexpensive screening techniques to identify and reject high alkaloidplant material, development of suitable tools is important challenges for lupins breeding and seed production.The aim of this study was to compare two alkaloid content estimation methods in Lupinus angustifoliusL. and Lupinus albus L.During the Wagner’s colorimetric test, which is recommended by the UPOV, seed halves were stained onfour colors depending on the alkaloid content but only the level of 0.5% – 0.6% showed clear color change.Gas chromatography allowed accurate quantification and qualification of alkaloid content.Since safe alkaloid content for consumption is 0.02% of seed dry weight, colorimetric method is less usefulfor dividing lupin cultivars into sweet and bitter, than gas chromatography but can be used as a screeningtechnique
GENETIC RESOURCES OF TRITICALE IN THE POLISH GENE BANK
Get PDFThe aim of the research, conducted at the Institute of Plant Genetics, Breeding and Biotechnology of the University of Life Sciences in Lublin since 1982, is the systematic gathering and multi-annual characterization of triticale accessions collected in the Polish gene bank, as well as regeneration of materials preserved in the seed stores in case of reduced germination capacity or a small sample of grains. Valorisation of accessions is carried out in a 4-year cycle of field experiments and includes botanical identification and a description of morphological and useful traits (agricultural and qualitative), conducted every year according to the same methodology. The valorisation data include both traits determined in field evaluation (assessment of emergence, overwintering, lodging, fungal disease resistance, and heading and full maturity dates) and measurements of biometric features (plant height, spike length, number of spikelets per spike, number and weight of grains per spike, 1,000 grain weight, protein content in grain). As part of the research, accessions have been made available for use in breeding programmes and have been donated to research and development institutes as a broad spectrum of variation for genetic similarity analysis and to provide genetic sources of such traits as resistance to pre-harvest sprouting, response to toxic aluminium ions, and disease resistance
INFLUENCE OF THE MAGNETIC FIELD ON THE GERMINATION PROCESS OF TOSCA BEAN SEEDS (PHASEOLUS VULGARIS L.)
Get PDFThe experiment was aimed at determining, whether exposure to static magnetic fields of intensities (1T, 2.5T, and 5T) stimulates the growth of Tosca bean seeds (Phaseolus vulgaris L.). Each treatment group in the experiment underwent exposure to the magnetic field for a specific period of time, that is: 900s, 1800s, or 3600s. The measurements of root volumes were taken every 24 hours. The first measurement was taken after 96 hours since the beginning of germination. The collected data was analyzed using statistical methods. The experiment allowed to determine that the magnetic field had a significant influence on the lowering of bean seeds germination rate. The extent of this influence was dependent on the combination of factors: magnetic field force- time of exposure
Badania odporności mieszańców międzygatunkowych z plemienia Brassiceae na porażenie powodowane przez patogeny Leptosphaeria sp. i Alternaria sp.
Get PDFThe paper presents the results of studies on resistance of interspecies hybrids from the Brassiceae caused by Leptosphaeria sp. and Alternaria sp. pathogens. 159 intercross hybrid genomes were obtained, of which 52 genotypes were cloned in vitro and in vivo. The genotypes were searched from the Brassiceae less resistant to infestation by Leptosphaeria sp. and Alternaria sp. 49 oilseed rape lines were tested, of which a small proportion showed increased resistance to both pathogens. DNA analyses of ten pathogens used for inoculation were also performed. The resistance of seedlings (infection index for the 12 objects) obtained from interspecific hybrids and also control and donor plants of oilseed rape were tested for infection caused by pathogens of the genus Leptosphaeria and Alternaria. Selected genotypes with low infection indices were transferred to resistance breeding.W pracy przedstawiono wyniki badań dotyczące odporności mieszańców międzygatunkowych z plemienia Brassiceae na porażenie powodowane przez patogeny Leptosphaeria sp. oraz Alternaria sp. Przedstawiono problematykę dotyczącą wytwarzania mieszańców międzygatunkowych techniką in vitro. Otrzymano 159 zarodków mieszańców międzygatunkowych, z których 52 genotypy klonowano in vitro i in vivo dla otrzymania roślin w większej wydajności. Poszukiwano genotypów z plemienia Brassiceae odpornych na porażenie powodowane przez Leptosphaeria sp. oraz Alternaria sp. Przebadano 49 obiektów, z których mała część wykazywała podwyższoną odporność na oba patogeny. Wykonano także analizy DNA dziesięciu patogenów użytych do inokulacji. Badano także odporność siewek (indeks porażenia dla 12 obiektów) otrzymanych z mieszańców międzygatunkowych oraz roślin kontrolnych i donorowych rzepaku, na porażenie powodowane przez patogeny z rodzaju Leptosphaeria oraz Alternaria. U wybranych genotypów odnotowano niski indeks porażenia (IP) i te formy przekazano do dalszej hodowli odpornościowej