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Key ncRNAs in breast cancer subtypes associated with apoptosis and RNA methylation modification.
No full text(A). Key ncRNAs in breast cancer subtypes show correlation with apoptosis-related genes. (B) Key ncRNAs in breast cancer subtypes show correlation with m1A modification-related genes. Red represents positive correlation while blue represents negative correlation. (C) Key ncRNAs in breast cancer subtypes show correlation with m5C modification-related genes. Red represents positive correlation while darkblue represents negative correlation. (D) Key ncRNAs in breast cancer subtypes show correlation with m6A modification-related genes. Orange represents positive correlation while blue represents negative correlation.</p
A model based on the short noncoding RNA’s expression, and the age and sex of the patient for prediction of CD and UC development from IBDU.
No full text(A) PCA plot showing samples clustered based on the model into IBDU-CD (green) and IBDU-UC (pink). IBDU-IBDU samples shown in blue color show a tendency towards IBDU-CD or IBDU-UC. (B) ROC plot showing prediction based on 3 individual candidate’s expression and the model based on the relative expression values of miR-182-5p, miR-451a, and ENSG00000239080 (SnoU13) along with the age at diagnosis and sex of the patients.</p
Fig 4 -
No full textThe effect of FSH and LH at doses of 50–150 ng/ml on ITLN1 protein expression (A) and its concentrations (B) in the culture medium of coculture of ovarian Gc and Th from LW and MS pigs on days 10–12 of the oestrous cycle. The protein expression was detected by western blot assay; each protein abundance was evaluated densitometrically and expressed as the ratio relative to ACTB abundance. ITLN1 concentration was determined by ELISA assay. Results were reported as the mean ± SEM of five independent determinations. The lower case letter denotes differences between hormones doses whereas an upper case letter denotes differences between types of pig breeds (p < 0.05; one-way ANOVA followed by Tukey post hoc test). ACTB, actin beta; ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; FSH, follicle-stimulating hormone; LH, luteinizing hormone; LW, Large White; MS, Meishan; SEM, standard error of the mean; Tukey’s HSD, Tukey’s honest significant difference.</p
Standardised effect of two SDs of possession duration for the variability on collective tactical variables.
No full textFor the team on attack (filled circles) and defence (open circles). Circles towards the left indicate higher in short possession and the right higher in long possession.</p
Changes realtive to baseline (t = 0) in the concentration of biomarkers—intestinal fatty acid-binding protein (IFABP), syndecan-1, lipopolysaccharide-binding protein (LBP), soluble CD14 (sCD14) and LBP:sCD14 ratio—at 2, 6 and 24 hours after saline injection (Control) vs after a high fat high carbohydrate (HFHC) meal.
No full textIFABP levels were lower 6 hours after a HFHC meal intake compared with those following saline injection with a post HFHC IFABP median level of -224.8 (IQR -579.8 to -114.8) pg/ml vs post saline injection of 76.3 (-418 to 634.3) pg/ml (p = 0.04; Wilcoxon), although statistical significance was lost if the data were corrected for multiple comparisons. No other differences for biomarkers at any of the other time points were identified.</p
CONSORT 2010 checklist of information to include when reporting a randomised trial*.
No full textCONSORT 2010 checklist of information to include when reporting a randomised trial*.</p
3D Gaussian Geometric Moment Invariants
No full text3D moment invariants are important tools for 3D image feature representation. In this paper, we introduced a novel approach for constructing 3D moment invariants using Gaussian geometric moments. Our proposed method demonstrated invariance under translation, rotation, and scale transformations. The numerical experiments validate the invariance and robustness of the proposed method, comparing it with traditional 3D geometric moments and revealing superior performance in the presence of noise and transformations. Additionally, the method is applied to content-based 3D image retrieval, exhibiting promising results through Minkowski distance-based retrieval on the Princeton Shape Benchmark (PSB) database.</p
MDSC-targeted liposomal all-trans retinoic acid suppresses mMdscs and improves immunotherapy in HBV infection
No full textMyeloid-derived suppressor cells (MDSCs) are evolving as a prominent determinant in cancer occurrence and development and are functionally found to suppress T cells in cancer. Not much research is done regarding its involvement in viral infections. This research was designed to investigate the role of MDSCs in hepatitis B virus (HBV) infection and how targeting these cells with our novel all-trans retinoic acid encapsulated liposomal formulation could improve immunotherapy in C57BL/6 mice. Ten micrograms (10 μg) of plasmid adeno-associated virus (pAAV/HBV 1.2, genotype A) was injected hydrodynamically via the tail vein of C57BL/6 mice. An all-trans retinoic acid encapsulated liposomal formulation (L-ATRA) with sustained release properties was used in combination with tenofovir disoproxil fumarate (TDF), a nucleotide analog reverse transcriptase inhibitor (nRTI) to treat the HBV infection. The L-ATRA formulation was given at a dose of 5 mg/kg intravenously (IV) twice a week. The TDF was given orally at 30 mg/kg daily. Our results revealed that L-ATRA suppresses MDSCs in HBV infected mice and enhanced T-cell proliferation in vitro. In vivo studies showed higher and improved immunotherapeutic effect in mice that received L-ATRA and TDF concurrently in comparison with the groups that received monotherapy. Lower HBV DNA copies, lower concentrations of HBsAg and HBeAg, lower levels of ALT and AST and less liver damage were seen in the mice that received the combination therapy of L-ATRA + TDF. In effect, targeting MDSCs with the combination of L-ATRA and TDF effectively reduced mMDSC and improved immunotherapy in the HBV infected mice. Targeting MDSCs could provide a breakthrough in the fight against hepatitis B virus infection.</p
Videos 01-14
No full textVideo 1Human myotubes differentiated with IGF1 representative for best contraction at 10x magnificationVideo 2Human myotubes differentiated with IGF1 representative for best contraction at 10x magnificationVideo 3Human myotubes differentiated with IGF1 representative for average contraction at 10x magnificationVideo 4Human myotubes differentiated with IGF1 representative for average contraction at 10x magnificationVideo 5Human myotubes differentiated with IGF1 representative for worst contraction at 10x magnificationVideo 6Human myotubes differentiated with IGF1 representative for worst contraction at 10x magnificationVideo 7Human myotubes differentiated with IGF1 and visible striation at 30x magnificationVideo 8Human myotubes differentiated without IGF1 representative for average at 10x magnificationVideo 9Human myotubes differentiated without IGF1 representative for average at 10x magnificationVideo 10Human myotubes differentiated without IGF1 representative for best at 10x magnificationVideo 11Human myotubes differentiated with IGF1 showing spontaneous contraction at 10x magnificationVideo 12Human myotubes differentiated with IGF1 showing spontaneous contraction at 10x magnificationVideo 13Human myotubes differentiated with IGF1 spontaneously contracting followed by EPS at 10x magnificationVideo 14Human myotubes differentiated with IGF1 showing tetanic contraction at 30x magnification</p
Monolayer Arrays of Au Nanoparticles on Block Copolymer Brush Films for Optical Devices and Biosensors
No full textGold nanoparticle (AuNP) monolayers possess unique optical
properties
and are widely used in optical devices and biosensors, sometimes necessitating
dense but nonclose-packed monolayers. Current self-assembly methods
are generally limited to small surfaces and are often plagued by AuNP
aggregation. Here, we use a facile method, suitable for substrates
of any size or form, to produce dense, unaggregated, randomly packed
AuNP monolayers on brushlike films of polystyrene-block-poly(4-vinylpyridine) (PS-P4VP) obtained by dip-coating flat surfaces
from very dilute solutions, where PS forms the brush, and the citrate-stabilized
AuNPs interact with the P4VP anchoring layer when the template is
incubated in an AuNP colloid. By investigating the effect of molecular
weight (Mn), dip-coating solvent, and
colloid pH on the characteristics of the adsorbed films and the subsequent
deposition of AuNPs, we constructed a morphology map in terms of PS Mn and chain grafting density under favorable
pH conditions, showing the region that yields the desired dense well-dispersed
AuNP monolayers. Above an upper PS Mn boundary
(around 50–60 kg/mol), the PS brushcontinuous or in
the form of patchesis too thick and rigid for the AuNPs to
access the underlying P4VP and is also subject to kinetic effects,
thus causing little or nonuniform, even aggregated, AuNP deposition.
These findings have important implications for the future employment
of dense, unaggregated AuNP monolayers in miniature sensors and as
optical coatings in a wide range of optical devices