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162 research outputs found
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Subclinical myocardial dysfunction in patients recovered from COVID-19
No full textdoi: 10.1111/echo.15215
Myocardial injury (MI) can be detected during the acute phase of Coronavirus disease 19 (COVID-19) and is associated with a dismal prognosis. Recent imaging studies described the persistence of cardiac abnormalities after the recovery. The aim of the study was to investigate the spectrum of cardiac abnormalities at mid-term follow-up in patients recovered from COVID-19 using clinical assessment, laboratory tests and imaging evaluation with comprehensive echocardiography.1 The study included 123 patients divided into two groups according to the presence of MI during hospitalization. At 3 months follow-up patients with prior MI showed lower values of global longitudinale strain, higher frequency of8 impaired diastolic funcion and higher values of pulmonary pressure. Echocardiography with speckle-tracking analysis may be a usefull tool to identify subclinical myocardial dysfunction in patients recovered from COVID-19
Expanded circulating hematopoietic stem/progenitor cells as novel cell source for the treatment of TCIRG1 osteopetrosis
No full textPMID: 31949009 PMCID: PMC7776247 DOI: 10.3324/haematol.2019.238261
Abstract
Allogeneic hematopoietic stem cell transplantation is the treatment of choice for autosomal recessive osteopetrosis caused by defects in the TCIRG1 gene. Despite recent progress in conditioning, a relevant number of patients are not eligible for allogeneic stem cell transplantation because of the severity of the disease and significant transplant-related morbidity. We exploited peripheral CD34+ cells, known to circulate at high frequency in the peripheral blood of TCIRG1-deficient patients, as a novel cell source for autologous transplantation of gene corrected cells. Detailed phenotypical analysis showed that circulating CD34+ cells have a cellular composition that resembles bone marrow, supporting their use in gene therapy protocols. Transcriptomic profile revealed enrichment in genes expressed by hematopoietic stem and progenitor cells (HSPCs). To overcome the limit of bone marrow harvest/ HSPC mobilization and serial blood drawings in TCIRG1 patients, we applied UM171-based ex-vivo expansion of HSPCs coupled with lentiviral gene transfer. Circulating CD34+ cells from TCIRG1-defective patients were transduced with a clinically-optimized lentiviral vector (LV) expressing TCIRG1 under the control of phosphoglycerate promoter and expanded ex vivo. Expanded cells maintained long-term engraftment capacity and multi-lineage repopulating potential when transplanted in vivo both in primary and secondary NSG recipients. Moreover, when CD34+ cells were differentiated in vitro, genetically corrected osteoclasts resorbed the bone efficiently. Overall, we provide evidence that expansion of circulating HSPCs coupled to gene therapy can overcome the limit of stem cell harvest in osteopetrotic patients, thus opening the way to future gene-based treatment of skeletal diseases caused by bone marrow fibrosis
RAB39B-mediated trafficking of the GluA2-AMPAR subunit controls dendritic spine maturation and intellectual disability-related behaviour
No full textraw data supporting figures in the manuscript doi.org/10.1038/s41380-021-01155-
A PGE2-MEF2A axis enables context-dependent control of inflammatory gene expression
No full textProstaglandin E2 (PGE2) modulates macrophage activation during homeostasis and disease, but the underlying mechanisms remain incompletely characterized. Cilenti, Barbiera, et al. reveal that PGE2 suppresses inflammatory gene expression by targeting a set of inflammatory gene enhancers marked by MEF2A, a critical regulator of type I interferon induction.
DOI: https://doi.org/10.1016/j.immuni.2021.05.016
Raw and analyzed RNA-Seq data: This paper, ArrayExpress: E-MATB-9275
Raw and analyzed scRNA-Seq data: This paper, ArrayExpress: E-MATB-9253
Raw and analyzed ChIP-Seq data: This paper, ArrayExpress: E-MATB-9254
Raw and analyzed ATAC-Seq data: This paper, ArrayExpress: E-MATB-925
Neuropsychological deficits correlate with symptoms severity and cortical thickness in Borderline Personality Disorder
No full textNeuropsychological abnormalities have been proposed to contribute to the development and
maintenance of Borderline Personality Disorder (BPD). In this study we compared the performance of 24 BPD patients to 24 healthy controls obtained at the
neuropsychological battery “Brief Assessment and Cognition in Schizophrenia”, exploring the relationship between
the cognitive impairments and current symptomatology, brain grey matter volumes and cortical thickness,
controlling for medications load.
Biological data are not uploaded due to ethical restrictions.
https://doi.org/10.1016/j.jad.2020.09.06
A virtuous cycle operated by ERp44 and ERGIC-53 guarantees proteostasis in the early secretory compartment
No full textThe composition of the secretome depends on the combined action of cargo receptors that facilitate protein transport and sequential checkpoints that restrict it to native conformers. Acting after endoplasmic reticulum (ER)-resident chaperones, ERp44 retrieves its clients from downstream compartments. To guarantee efficient quality control, ERp44 should exit the ER as rapidly as its clients, or more. Here, we show that appending ERp44 to different cargo proteins increases their secretion rates. ERp44 binds the cargo receptor ER-Golgi intermediate compartment (ERGIC)-53 in the ER to negotiate preferential loading into COPII vesicles. Silencing ERGIC-53, or competing for its COPII binding with 4-phenylbutyrate, causes secretion of Prdx4, an enzyme that relies on ERp44 for intracellular localization. In more acidic, zinc-rich downstream compartments, ERGIC-53 releases its clients and ERp44, which can bind and retrieve non-native conformers via KDEL receptors. By coupling the transport of cargoes and inspector proteins, cells ensure efficiency and fidelity of secretion.
DOI: 10.1016/j.isci.2021.10224
Data for "B1 lymphocytes develop independently of Notch signaling during mouse embryonic development"
No full textB1 lymphocytes are a small but unique component of the innate immune-like cells. However, their ontogenic origin is still a matter of debate. Although it is widely accepted that B1 cells originate early in fetal life, whether or not they arise from hematopoietic stem cells (HSCs) is still unclear. In order to shed light on the B1 cell origin, we set out to determine whether their lineage specification is dependent on Notch signaling, which is essential for the HSC generation and, therefore, all derivatives lineages. Using mouse embryonic stem cells (mESCs) to recapitulate murine embryonic development, we have studied the requirement for Notch signaling during the earliest B-cell lymphopoiesis and found that Rbpj-deficient mESCs are able to generate B1 cells. Their Notch independence was confirmed in ex vivo experiments using Rbpj-deficient embryos. In addition, we found that upregulation of Notch signaling induced the emergence of B2 lymphoid cells. Taken together, these findings indicate that control of Notch signaling dose is crucial for different B-cell lineage specification from endothelial cells and provides pivotal information for their in vitro generation from PSCs for therapeutic applications.
DOI: 10.1242/dev.19937
Predictive factors of radio-induced complications in 194 eyes undergoing gamma knife radiosurgery for uveal melanoma. DOI: 10.1111/aos.14814
No full textPredictive factors of radio-induced complications in 194 eyes undergoing gamma knife radiosurgery for uveal melanom
ACHIEVING VIROLOGICAL CONTROL IN PAN-RESISTANT HIV-1 INFECTION: A CASE SERIES
No full textWe report findings from heavily treatment-experienced PLWH with a pan-resistant HIV-1 infection, who achieved virological control once introduced injections of ibalizumab, that is free from cross-resistance with all the antiretroviral drugs available and ensures patient adherence due to a close monitoring attributable to the route of administration, combined with recycled enfuvirtide and an optimized background regimen, selected on the basis of an accurate evaluation of resistance mutations.
Please refer to DOI: https://doi.org/10.1016/j.ebiom.2022.10390
An introduction to Open Science and Research Data Management
No full text"An introduction to Open Science and Research Data Management" by the UniSR/OSR Open Science Team.
The presentation is divided in two parts:
- The first briefly discusses the core concepts and principles of Open Science, the benefits of this approach for all stakeholders, and the open science requirements from journals and funders, with a focus on the new policies adopted by the European Commission in the "Horizon Europe" programme (the EU's key funding programme for research and innovation, until 2027).
- The second part focuses on Research Data Management and provides practical information on Data Management Plans (DMPs), on how to effectively preserve and share research data, and on how to make research data FAIR (Findable, Accessible, Interoperable, Reusable).
All relevant references are collected in the last slide