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Conception de systèmes automatiques
No full textCet ouvrage propose une large introduction à toutes les connaissances nécessaires à la conception des systèmes automatiques, qu'il s'agisse de l'emploi des moyens analogiques et digitaux, des méthodes d'analyse et de synthèse théoriques ainsi que des processus à automatiser.LEI558 pages, 16x24 cm, 330 figures et tableau
The cysteine desulfurase IscS of Mycobacterium tuberculosis is involved in iron sulfur cluster biogenesis and oxidative stress defence
No full textThe complex multiprotein systems for the assembly of protein-bound iron sulfur (Fe S) clusters are well defined in Gram-negative model organisms. However, little is known about Fe S cluster biogenesis in other bacterial species. The ISC (iron sulfur cluster) operon of Mycobacterium tuberculosis lacks several genes known to be essential for the function of this system in other organisms. However, the cysteine desulfurase IscSMtb (Rv number Rv3025c; Mtb denotes M. tuberculosis) is conserved in this important pathogen. The present study demonstrates that deleting iscSMtb renders the cells microaerophilic and hypersensitive to oxidative stress. Moreover, the Delta iscSMtb mutant shows impaired Fe S cluster-dependent enzyme activity, clearly indicating that IscSMtb is associated with Fe S cluster assembly. An extensive interaction network of IscSMtb with Fe S proteins was identified, suggesting a novel mechanism of sulfur transfer by direct interaction with apoproteins. Interestingly, the highly homologous IscS of Escherichia coli failed to complement the Delta iscSMtb mutant and showed a less diverse protein-interaction profile. To identify a structural basis for these observations we determined the crystal structure of IscSMtb, which mirrors adaptations made in response to an ISC operon devoid of IscU-like Fe S cluster scaffold proteins. We conclude that in M. tuberculosis IscS has been redesigned during evolution to compensate for the deletion of large parts of the ISC operon.UPCO
Surface-grafting of hydrophilic polymer brushes on silicon substrates and their stability in aqueous media
No full textSCGCGR-53 1027; EPFL STI IMX LP. Consultable sur demande à la Bibliothèque de l'EPFL / Offered in consultation at the EPFL library
NMR spectroscopy and DFT calculations of a self-assembled arene ruthenium rectangle obtained from a combination of coordination and hydrogen bonds
No full textThe hydrogen-bonded arene ruthenium metalla-rectangle, [(p-cymene)(2)Ru-2(OOnOO)(UPy)(2)](2)(4+), obtained from 1-(4-oxo-6-undecyl-1,4-dihydropyrimidin-2-yl)-3-(pyridin-4-ylmethyl)urea (UPy) and the dinuclear arene ruthenium clip (p-cymene)(2)Ru-2(OOnOO)Cl-2 (OOnOO = 2,5-dioxido-1,4-benzoquinonato), is investigated by means of solution-phase NMR spectroscopy. Rotating frame nuclear Overhauser effect measurements are used to probe the H-bond network that drives the UPy self-assembly as well as the full rectangular supramolecular system. An effective distance that takes into account both intra-and intermolecular polarization-transfer pathways is utilised for data analysis. The experimental findings are corroborated by DFT calculations of NMR parameters and internuclear distances, thus confirming the formation of a very stable tetranuclear metalla-assembly.LRM
Screening and biophysical characterization of small-molecule stabilizers of WT-p53
No full textSCGCGR-53 623; EPFL - SB - ISIC - LIP; University of Cambridge - Centre for Protein Engineering. Consultable sur demande à la Bibliothèque de l'EPFL / Offered in consultation at the EPFL library
