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A new role for Wilms Tumor protein 1: differential activities of + KTS and -KTS variants to regulate LHβ transcription
No full textLuteinizing hormone (LH) is synthesized and secreted throughout the reproductive cycle from gonadotrope cells in the anterior pituitary, and is required for steroidogenesis and ovulation. LH contains an α-subunit common with FSH, and a unique LHβ subunit that defines biological activity. Basal LHß transcription is low and stimulated by hypothalamic GnRH, which induces synthesis of early growth response protein-1 (Egr1), and stimulates binding of transcription factors Egr1 and sterodogenic factor-1 (SF1) on the promoter. WT1 (Wilms tumor protein1) is a zinc finger transcription factor with an essential role in urogenital system development, and which regulates several reproductive genes via interactions with SF1 or binding to GC-rich elements such as Egr1 binding sites. We investigated a potential role for WT1 in LHβ transcription in clonal mouse gonadotrope LβT2 cells. WT1 was present in LβT2 and mouse pituitary cells, and protein bound to the endogenous LH promoter.
Interestingly, mRNAs for WT1, which contains a three amino-acid insertion between the 3rd and 4th zinc fingers, and the WT1 (-KTS) variant were both expressed at significant levels. WT1 mRNAs and protein were decreased approximately 50% by GnRH treatment, under conditions where Egr1 mRNA and protein, and LHß transcription, were stimulated. Decreasing expression of mRNA for WT1 (-KTS) decreased stimulation of LHß and Egr1 by GnRH, whereas decreasing both WT1 (- KTS) and (KTS) increased endogenous LH transcription, and prevented LH but not Egr1 stimulation by GnRH, suggesting differing biological activities for the WT1 isoforms. Overexpression of WT1 showed that WT1 enhanced LHß promoter GnRH stimulation 2-to-3-fold and required the 3’Egr1 site, but WT1 repressed both basal and GnRH-stimulated LHβ promoter activity by approximately 70%. Our
data suggest that WT1 can modulate LHβ transcription, with differential roles for the two WT1 variants; WT1 (-KTS) enhances and WT1 (KTS) suppresses transcriptio
Main ANSYS CFX file to produce transient thrust and input power for a pitching airfoil with NACA 0012 cross-section
No full textThe main ANSYS CFX file is attached. The code was used to produce cycle-averaged thrust and input power coefficients for a pitching foil with NACA 0012
Survey on Humanities Graduate Education and Alternative Academic Careers (Employer Survey)
No full textAs humanities scholars increasingly recognize the value of public engagement, and as the proportion of tenure-track faculty positions available to new graduates continues to decline, many humanities programs are focusing renewed attention on equipping graduate students for careers as scholars both within and beyond academe. To support those efforts, the Scholarly Communication Institute has carried out a study investigating perceptions about career preparation provided by humanities graduate programs. The survey results help to create a more solid foundation on which to base curricular reform and new initiatives by moving the conversation about varied career paths from anecdote to data.
The study consisted of two main phases: one public, one confidential. The first phase involved creating an exploratory public database of self-identified alternative academic practitioners. The database was built within the framework of the #Alt-Academy project (http://mediacommons.futureofthebook.org/alt-ac/) in order to leverage the energy of existing conversations. The second phase comprised two confidential surveys. The primary survey targeted people with advanced humanities degrees who self-identify as working in alternative academic careers, while a second targeted employers that oversee employees with advanced humanities degrees. Because we were working with a somewhat nebulous population, our subsequent distribution focused on “opt-in” strategies—especially social media, listervs, and traditional media coverage. While this method has limitations, we hoped to learn something not only from the content of the responses, but from the number and type of respondents.
The data obtained through this study represents an important step towards identifying and understanding the career preparation needs of humanities graduate students by examining particular issues facing the increasingly visible and vocal population of humanities graduates in alternative academic careers. Equipping graduate students with the skills and literacies needed for 21st century scholarly work—from technical fluency to an understanding of organizational structures—is critical to ensuring continued rigorous and creative research, scholarship, and teaching
McCormick Observatory
No full text3D laser scan data of the McCormick Observatory, Mount Jefferson aka Observatory Hill, University of Virginia; contains data from main survey of 2015-04-08 and 15; also follow up data collected on 2023-04-19 of the main dome without canvas covering; data collected with FARO Focus X130 laser scanners; data processed with FARO Scene v. 2023.0.1
Fralin - Euboean Hydria
No full textFralin 3D ; Laser scan data of greek vase from the Fralin Museum - Euboean Hydria; Creaform Zscanner 700 CX ; 3D mesh dat
VCAP Longitidunal Dataset
No full textVCAP Dataset is the product of "Short-term cognitive change in adults from 18 to 80", an NIH-funded project directed by Dr. Timothy Salthouse between 2001 and 2019. The dataset contains longitudinal assessments from about 6000 individuals aged 19-99 years old. The availability of the dataset is curated by project transition team after the retirement of Dr. Salthouse.
We request that the guestbook to be signed before downloading the datasets. It is also expected that users are committed (1) to using the data only for research purposes and not to identify any individual participant; (2) to securing the data using appropriate computer technology; and (3) to citing the VCAP appropriately if the use of data leads to new publications or other products