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Discovering Dengue Virus Antibody Using Phage Display Technology
No full textDengue virus (DENV) is a type of flavivirus transmitted globally by the Aedes aegypti mosquito and is the causal agent of Dengue fever. Due to global warm-ing and rising temperatures worldwide, the rate of exposure to DENV is now reaching unprecedented levels, with over 400 million people getting infected each year. Despite worldwide attention to DENV, there is not currently an effective treatment or vaccine. Here, we focused on developing an antibody for the NS1 protein of the DENV, which enables earlier detection of disease and could further decrease the rate of death. Our study utilized a method more time-saving and cost-effective than the traditional method of extracting antibodies from the blood or B-cells of infected animals that show immu-nity: Phage Display. This technique allowed us to derive monoclonal antibodies (mAbs) from the genome library and select DENV antibodies that bind to antigen proteins. In this study, we used Phage Display to select ninety-six potential antibodies from three phases of panning, which then went through indirect ELISA screening for optical density (OD) confirmation (OD > 1.5). Sixteen of the high optical density antibodies were sent to a DNA sequencing analysis facility where fifteen of them were revealed to be the same 1G1 strain. By understanding how antibodies are discovered using phage display technology, we hope to advance the field with reevaluation of current vaccines and the development of novel ones
Effect of Mathematical Games on Senior Secondary School Students’ Achievement in Mathematics According to Gender: Effect of Mathematical Games on Students’ Achievement in Mathematics According to Gender
No full textPlaying mathematical games helps many senior secondary school students—especially girls—acquire basic mathematical skills, but it can be difficult. Thus, this study examined how gender-related mathematical gameplay affects secondary school students' performance. The design of the study was quasi-experimental. Purposively, a sample of fifty senior secondary school students from the Federal Capital Territory (FCT) of Nigeria's Abuja Council Area was chosen. Using a coin toss, the researcher randomly allocated intact classes to the experimental and control groups. An algebraic achievement test administered before and after the treatment determined the students' achievement level. T-tests, means, and standard deviation were used to analyze the data. According to the study, among students who played mathematics games, female students had a higher mean score than male students. The findings imply that mathematical games enhance mathematics teaching and learning and should thus be encouraged and used by teachers to introduce concepts in mathematics to students at different levels, irrespective of their gender
Can Induced Awe Reduce Anti-Gay Prejudice in Heterosexual Adults and Does the Need for Closure Moderate this Effect?
Get PDFWith sexual prejudice continuing to be widely prevalent and seriously harmful, there is a need to find ways to reduce anti-gay prejudice (AGP). This online experimental study examined if a novel intervention, induced awe, can reduce AGP; if those high in need for closure (NFC) show higher AGP; and if NFC moderates the effect of awe on AGP. In total, 154 heterosexual adults completed the Need for Closure Scale (Roets & Van Hiel, 2011) before being randomly assigned to one of three emotion inducement interventions – 1) watching a 4.43 min long video of the target emotion, awe, 2) watching the comparison emotion, amusement, or 3) watching a neutral emotion as a control. Post intervention, the participants completed the explicit measures Homosexuality Attitudes Scale (HAS; Kite & Deaux, 1986). Data were analysed using a 2 (NFC) x 3 (emotion type) independent factorial ANOVA. None of the three hypotheses were supported since there were no main effects of awe or NFC on AGP, and no interaction effect of awe and NFC on AGP. Key implications of these results were 1) awe inducement does not change prejudicial attitudes at an explicit level (Dale et al., 2020), 2) factors beyond NFC, like Right Wing Authoritarianism (RWA) and Social Dominance Orientation (SDO), may influence the effectiveness of awe in reducing prejudice, and 3) questions were raised about certain boundaries for awe’s effectiveness. Methodological modifications suggested for future research include using implicit measures or veiled elicitation methods for authentic measurement of AGP, employing more potent awe elicitors, and assessing the mediating role of RWA and SDO on the effect of NFC on AGP
Front matter: Letter from the Editors, Masthead, and Contents
No full textFront matter for the Columbia Journal of Undegraduate Mathematics Volume 1 Issue 1. Contains the Letter from the Editors, Masthead, and Contents
Indigenous Peoples in French Guiana: will France ever recognize their right to self-determination, land, territory, biodiversity and Free, Prior and Informed Consent?
Get PDFFrench Guiana, located in South America, hosts over 10,000 Indigenous Peoples, principally the Kali’na Tileuyu, Lokono and Pahikweneh, Wayãpi, Teko and Wayana people. Despite making up around 4% of the total population, Indigenous Peoples in French Guiana continue to face systematic challenges in being able to fully enjoy their human rights and rights as Indigenous Peoples. As an overseas territory of France, French Guiana falls under the governance of France and is fully integrated as an overseas department, separate from other overseas territories such as New Caledonia. This full integration has classified Indigenous Peoples as French People, making it not only difficult to get estimates of the actual number of Indigenous Peoples in French Guiana as the French Constitution prohibits ethnic statistics, but also prevent the ability of Indigenous Peoples to obtain their right to self-determination and control over their own land as provided in the United Nations Declaration on the Rights of Indigenous Peoples (UNDRIP). Therefore, this paper will discuss the violations of the UN Declaration on the Rights of Indigenous Peoples committed by France that hinders their attainment to their fundamental freedoms and basic human rights. To do so, this paper will firstly begin by discussing the implementation of the UNDRIP in French Guiana in reference to several articles. In explaining the various articles, it will demonstrate how the French government’s recent actions continue to lead to the destruction of Indigenous territories and threaten their survival. Moreover, this paper will focus on articles 3, 10, 25, 29 which pertain to the right to self-determination, the right to land and territories, and the right to protect biodiversity. These rights alongside the right to Free, Prior and Informed Consent (FPIC) should allow Indigenous Peoples to exercise control over decisions that essentially affect not only their way of life but also their existence, particularly considering the history of Indigenous Peoples that have frequently been the “first victims of development activities.
Dementia Research: Responding to the Need Through Autonomous Choice
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ABSTRACT
Dementia is progressive and is characterized by fluctuating cognition, which presents challenges in the assessment of decision-making capacity and, ultimately, for informed consent. The responsibility for ethical research combined with the fluctuating cognition associated with dementia necessitates using a flexible decision-making and capacity assessment process that respects the autonomy of the participant, the uneven decline associated with the disease process, and responds to the urgent need for increased participation in studies.
INTRODUCTION
Dementia, a common neurodegenerative disorder, is a leading cause of death worldwide and is estimated to affect more than 55 million people.[1] In the United States, 1 in 9 (6.7 million) people aged 65 and older have Alzheimer’s disease—the most common type of dementia.[2] While dementia is a research priority and the National Institutes of Health funding for it exceeds $3.7 billion annually, further clinical research and research participants are needed to understand and identify new approaches to prevent, diagnose, and treat dementia.[3] A significant barrier to research is the recruitment of persons with dementia as participants, specifically because of challenges in evaluating decision-making capacity and obtaining truly informed consent.[4]
Continued successful advocacy for additional dementia research requires more research at all stages of the dementia disease process[5] to better understand how and why the brain changes.[6] To reduce the physical, psychological, and financial impact on individuals and society, this research should include drug trials, medical devices, diagnostic tools, and both behavioral and lifestyle changes.[7] A potential ethical solution is the use of a supported decision-making model for persons with dementia, which would not only reduce barriers to research participation but also promote individual autonomy.
BACKGROUND
While dementia is progressive, it is also characterized by fluctuating cognition, which presents challenges in the assessment of decision-making capacity and, ultimately, informed consent.[8] Meaningful consent requires that the person providing it has the capacity to do so, yet these capacities may be reduced by the cognitive impairment and fluctuation associated with dementia.[9] Cognitive fluctuation means that decision-making capacity not only changes during different situations and over time but also may be time and task-specific.[10] The responsibility for ethical research combined with the fluctuating cognition associated with dementia necessitates the use of a supported decision-making and capacity assessment process that respects the autonomy of the participant, the uneven decline associated with the disease process, and responds to the urgent need for increased participation in studies.
I. Research Participants and Barriers to Dementia Research
Increased investment in dementia research has increased the number of treatment, prevention, and care studies and has produced an urgent need to recruit and enroll participants in studies.[11] For example, Alzheimer’s clinical trials are seeking more than 270,000 participants, with only one in ten persons screened being eligible for participation.[12] Research participation poses challenges, with the top barriers being the participant (study partner) burden and lack of awareness and resources among primary care physicians.[13] Many studies require participation by those who can provide informed consent and adhere to study procedures. Yet many caregivers of the patient, such as a spouse or family member, are reluctant to participate in studies due to extensive time commitments and unwillingness to authorize extensive screening tests that may not help the participant and may even cause discomfort or harm.[14] Furthermore, research participation may have risks of drug side effects. Additionally, physician offices may not be equipped to perform dementia-related diagnostic assessments and screening and may be unaware of opportunities for research participation.[15]
Informed consent and the recruitment of persons with dementia for research is complex because of fluctuating and progressively diminishing capacity of persons with dementia.[16] The inability to make one type of decision does not mean that there is an overall lack of ability to make decisions. Persons with dementia, for instance, may not have the capacity to agree to a complex treatment program or surgery, but they may have the ability to decide to take a new medication or to get a flu shot.[17]
II. Capacity Assessment and Supported Decision-making
Decision-making capacity varies along a continuum and is affected by factors such as mood and motivation, yet the research review and approval processes require a categorical, binary determination about a continuous phenomenon.[18] Decision-making capacity is not always linear and includes factors such as mood, trust, and timing.[19] Informed consent and the associated assessment of decision-making capacity for research is determined by the investigator where the exposure to the potential participant over time is intermittent and limited, and where the researcher's goal is generalizable knowledge rather than treatment of the patient. Therefore, while the principal investigator should have the most comprehensive knowledge about the potential risks and harms of any drugs or procedures being performed as part of the research, the principal investigator might have limited knowledge of potential participant values, preferences, and goals and how the participant might view risks and harms.[20] Current literature demonstrates that while researchers are willing to support the decision of persons with dementia to participate or not, researchers are not equipped to assist in decision assistance and concrete decision support measures.[21] Further, decision assistance by the researcher may not be appropriate and could create a conflict of interest.
Persons with dementia should participate in decision-making about research participation, although they may lack decisional capacity when it comes to other decisions, including other healthcare decisions, and they may need support in making those decisions. If they understand the risks and the responsibilities of the research study, then they should be able to decide, even if a surrogate decision-maker has been identified. Surrogate decision-makers can fail to incorporate the nuanced context related to fluctuating capacity. Clinicians, surrogates, or other legally authorized representatives are oftentimes “gatekeepers” to potential research participants and may not make a decision that reflects what the patient would want.[22] Institutional review boards provide inadequate protection from misaligned decisions by surrogates because they also lack knowledge of the participant’s current values and preferences.[23] Due to the slow, progressive nature of dementia and the need to assess decision-making capacity for medical treatments early in the disease process, the default position may be overreliance on surrogate decision-making throughout the disease process for all decisions, even when the individual has some capacity and desire to make decisions autonomously.[24]
Capacity assessment is not an all-or-nothing proposition, and supported decision-making could enable persons with dementia to retain their ability to make autonomous choices.[25] Supported decision-making is a structured yet flexible process that fosters independence. As of 2024, 18 states plus the District of Columbia have passed supported decision-making laws.[26] Trusted advisors, called supporters, often selected by the individual, help the person understand, consider, and communicate decisions about areas where decision-making assistance may be needed, such as research participation.[27] Supporters, for example, would gather relevant information, including from the researcher and care provider, and provide information to the potential participants, enabling them to make their own decisions. The research participants would make decisions about when, where, and how capacity tests would be performed in addition to decisions about their medical care and research participation. Ideally, the selected test would be relevant to the risk of the intervention, decisional complexity, and consequences of the intervention and should be performed under optimal circumstances and conditions.[28] Supported decision-making helps fulfill the researcher’s obligation to assure adequate consent with a process that promotes autonomous participant decision-making rather than defaulting to a proxy or surrogate.
This collaboration between supporters and research participants should enhance the participant’s understanding of the risks and benefits of the research. Working together, clinicians, researchers, research participants, and relatives can mitigate challenges such as stakeholder biases, role confusion, and paternalistic behaviors.[29] An estimated 75 percent of caregiving for a patient with dementia is provided informally by friends and family.[30] This already existing informal network provides the foundation for a more structured, supported decision-making model that recognizes the strengths and capacities of various stakeholders and trusted advisors, with an aim to promote participant autonomy. These trusted advisors should know the individual’s values and goals and should assist the potential participant to express, not construct, their decisions.
Structured, supported decision-making with trusted advisors can be implemented on a case-by-case basis for individuals determined to have dynamic functional impairments. Implementation begins with the identification of the areas of assistance needed specific to the research study under consideration. Next, the persons who could serve as supporters should be identified. The individual should discuss the role with each potential supporter and any agreement should be documented.[31]
Supporters are selected by the beneficiary and are usually friends, family members, or experts who know and respect the individual’s will and preferences. The role of the supporter is to provide information to the individual so that the individual can decide. For example, if the research is social or behavioral in nature, and involves physical activity or interaction with other individuals, a friend who has knowledge of the individual’s daily activities could provide guidance as to an optimal time of day for participation. If the research involves medical testing, a family member could gather and present pros and cons of the procedure to the individual. Most importantly, supporters are people who know and respect the values of the potential participant and commit to assisting the individual in making their own decision.
A supported decision-making model will not only increase much-needed research participation but will also promote the autonomous decision-making of the participant. This model for research participation decision-making shifts the process from a traditional assessment of decisional capacity to a study-specific assessment that engages trusted stakeholders earlier and throughout the participation period and assesses capacity with a relevant task at an optimal time.
III. Counterarguments to Supported Decision-making
Despite its benefits, supported decision-making has its limits and its risks. First, while dementia is characterized by fluctuating capacity, dementia is progressive and may require other surrogate approaches to decision-making, especially in the late stages of the disease. Surrogate or proxy decision-making may be appropriate for persons who consistently lack capacity, and the appropriateness of supported decision-making for persons with fluctuating capacity must be regularly evaluated and modified as needed. Second, no systematic method for determining whether a supporter has offered non-biased or non-controlling advice to the individual or if the individual’s decision has been properly represented or communicated has been identified. These challenges are not unique to supported decision-making, however. For example, surrogates may not easily identify or prioritize the relevant values associated with a particular decision, such as quality versus quantity of life. Further, a surrogate may make decisions based on personal desire for patient survival. Frameworks for value-based decision-making have been developed to support surrogates and to manage biases.[32] These frameworks could serve as a model for trusted stakeholders to adopt as part of the supported decision-making model. Third, the processes and roles of the stakeholders remain somewhat vague. The determination of which supporter is assigned to which decision or topic can be complex and requires good collaboration and communication among supporters and researchers. Through a supported decision-making model, an opportunity exists to blend the skills, expertise, and knowledge of all parties toward the benefit of appropriate research along with an effort to reinforce, rather than override, individual autonomy.
IV. Counterarguments to Encouraging Research Participation
There is a growing need to include persons with dementia in research. At the same time, many people may believe that research on individuals with diminished decisional capacity can place this vulnerable population at risk of physical, social, and psychological harm.[33] Further, this research may lead to drugs for future patients rather than the research subjects, many of whom would be nearing the end of life. Research undertaken with any vulnerable population raises concerns about how human dignity is both promoted and protected. However, failure to include this population in appropriate research can also contribute to the harm of individuals by slowing the development of evidence-based interventions.[34] Representation of the interests of individuals with dementia, along with the protection of their rights, is paramount when ensuring research is ethical. Researchers, institutional review boards, and individual decision-makers must adhere to ethical research practices. Rather than posing unacceptable risks to research participants, low-risk research performed ethically could benefit patients by allowing them to contribute to solutions. While research is generally for the good of others or society, some research would increase the possibility of new treatments during the research subject’s lifetime.
CONCLUSION
Research to prevent, diagnose, and cure dementia remains a national priority due to both the human and societal impact of dementia as well as the direct and indirect costs associated with dementia care. Research funding toward these ends continues to increase at a rate that exceeds available participants.[35] Research participation is always a choice. Informed consent is the gold standard by which researchers protect participant autonomy and prevent coercion and biases so that the potential participant can make an informed choice. The progressive and fluctuating capacity associated with dementia poses significant and unique challenges to informed consent and associated decisional capacity assessment. A principal investigator’s determination that a research protocol is minimal risk increases the likelihood that the research can ethically proceed, yet that determination has risks and challenges.
The fluctuating and, at times, impaired decisional capacity of persons with dementia makes recruiting participants difficult.[36] Different circumstances or conditions may demand different functional abilities—different tests of capacity based on risk.[37] Promoting autonomous decision-making in participants with impaired capacity does not necessarily require an alternate decision-maker but a supported decision-making structure that can adapt to the fluctuating capacity and risk of the decision.
A supported decision-making model that honors professional obligations and responsibilities, and brings together researchers, clinicians, caregivers, family, and friends in response to the fluctuating capacity of the potential participant is one way to increase research participation while respecting individual autonomy. The goal is to prioritize improved, autonomous decision-making for as long as possible so that persons with diminished decisional capacity can participate in dementia research—the right research at the right time with the right participant.
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[1] WHO. (2024). World Health Organization. https://www.who.int/news-room/fact-sheets/detail/dementia#:~:text=Key%20facts,nearly%2010%20million%20new%20cases.
[2] Association, A. s. (2024). Alzheimer's Association. https://www.alz.org/alzheimers-dementia/facts-figures
[3] NIH. (2022). National Institutes of Health, National Institute on Aging. Department of Health and Human Services. https://www.nia.nih.gov/research/blog/2022/07/looking-forward-nihs-alzheimers-disease-and-related-dementias-fy-2024-bypass#:~:text=For%20FY%202024%2C%20NIH%20estimates,dementias%20research%20totaling%20%243.87%20billion.
[4] Lepore, M. S., Sari B.;Wiener, Joshua M.;Gould, Elizabeth (2017). Challenges in Involving People with Dementia as Study Participants in Research on Care and Services. https://aspe.hhs.gov/reports/challenges-involving-people-dementia-study-participants-research-care-services-0
[5] Cohen-Mansfield, J. (2019). Who is informed and who uninformed? Addressing the legal barriers to progress in dementia research and care. Isr J Health Policy Res, 8(1), 17. https://doi.org/10.1186/s13584-018-0279-z
[6] NIA. (2020). The Urgent Need for Increased and Diverse Participation in Studies. United States Department of Health and Human Services Retrieved from https://www.nia.nih.gov/report-2019-2020-scientific-advances-prevention-treatment-and-care-dementia/urgent-need-increased
[7] Ibid.
[8] Trachsel, M., Hermann, H., & Biller-Andorno, N. (2015). Cognitive fluctuations as a challenge for the assessment of decision-making capacity in patients with dementia. Am J Alzheimers Dis Other Demen, 30(4), 360-363. https://doi.org/10.1177/1533317514539377
[9] Dunn, L. B., Nowrangi, M. A., Palmer, B. W., Jeste, D. V., & Saks, E. R. (2006). Assessing decisional capacity for clinical research or treatment: a review of instruments. Am J Psychiatry, 163(8), 1323-1334. https://doi.org/10.1176/ajp.2006.163.8.1323
[10] Trachsel, M., Hermann, H., & Biller-Andorno, N. (2015). Cognitive fluctuations as a challenge for the assessment of decision-making capacity in patients with dementia. Am J Alzheimers Dis Other Demen, 30(4), 360-363. https://doi.org/10.1177/1533317514539377
[11] NIA. (2020). The Urgent Need for Increased and Diverse Participation in Studies. United States Department of Health and Human Services Retrieved from https://www.nia.nih.gov/report-2019-2020-scientific-advances-prevention-treatment-and-care-dementia/urgent-need-increased
[12] NIA. (2018). Together We Make the Difference: National Strategy for Recruitment and Participation in Alzheimer’s and Related Dementias Clinical Research United States Government: United States Department of Health and Human Services Retrieved from https://www.nia.nih.gov/sites/default/files/2018-10/alzheimers-disease-recruitment-strategy-final.pdf
[13] Ibid.
[14] Ibid.
[15] Ibid.
[16] Trachsel, M., Hermann, H., & Biller-Andorno, N. (2015). Cognitive fluctuations as a challenge for the assessment of decision-making capacity in patients with dementia. Am J Alzheimers Dis Other Demen, 30(4), 360-363. https://doi.org/10.1177/1533317514539377
[17] Barstow, C., Shahan, B., & Roberts, M. (2018). Evaluating Medical Decision-Making Capacity in Practice. Am Fam Physician, 98(1), 40-46.
[18] Rosenstein, D. L. M., Franklin G. (2008). Research Involving Those at Risk for Impaired Decision-Making Capacity. In The Oxford Textbook of Clinical Research Ethics (pp. 437-445). Oxford University Press.
[19] Ibid.
[20] NIH. (2023). Office of Human Subjects Research Protections. US Government Office of Intramural Research. https://irbo.nih.gov/confluence/display/ohsrp/Researchers
[21] Wied, T. S., Poth, A., Pantel, J., Oswald, F., & Haberstroh, J. (2021). How do dementia researchers view support tools for informed consent procedures of persons with dementia? Z Gerontol Geriatr, 54(7), 667-675. https://doi.org/10.1007/s00391-020-01779-2 (Wie bewerten Demenzforscher:innen Entscheidungsassistenz für die informierte Einwilligung zur Forschungsteilnahme von Menschen mit Demenz?)
[22] Lepore, M. S., Sari B.;Wiener, Joshua M.;Gould, Elizabeth (2017). Challenges in Involving People with Dementia as Study Participants in Research on Care and Services. https://aspe.hhs.gov/reports/challenges-involving-people-dementia-study-participants-research-care-services-0
[23] Harrison, R. R. (2023). Legally Effective but Ethically Inadequate: Institutional Review Board Policies for Consent from Legally Authorized Representatives. Ethics Hum Res, 45(2), 14-25. https://doi.org/10.1002/eahr.500158
[24] Wilkins, J. M. (2018). Narrative Interest Standard: A Novel Approach to Surrogate Decision-Making for People With Dementia. Gerontologist, 58(6), 1016-1020. https://doi.org/10.1093/geront/gnx107
[25] Mezey, M., Teresi, J., Ramsey, G., Mitty, E., & Bobrowitz, T. (2000). Decision-making capacity to execute a health care proxy: development and testing of guidelines. J Am Geriatr Soc, 48(2), 179-187. https://doi.org/10.1111/j.1532-5415.2000.tb03909.x ; Representation, C. f. P. (2024). Supported Decision-Making. https://supporteddecisions.org/about-supported-decision-making/
[26] Representation, C. f. P. (2024). Supported Decision-Making. h
Creation of a ‘New Species’: A Decolonial Reading of Frantz Fanon and B.R Ambedkar
Get PDFThis paper argues that Fanon’s critique of colonisation and Ambedkar’s evaluation of the caste system share similarities because they collectively present a psycho-social understanding of the oppressed human condition. Both Fanon and Ambedkar argue that colonial and caste oppression has introduced a loss of cultural legacy, identity and capacity for self-determination. This loss is imprinted so deeply in the minds of the colonised and stakeholders of the caste system that a new species with a different social, political and economic consciousness is needed to transform and facilitate the formation of radical new social beliefs. For such a decolonisation of the mind to occur, Fanon proposes that violence—the capacity to resist and question—is fundamental, which I argue is also the foundation of Ambedkar’s solution to annihilate caste. However, violence in terms of Fanon and Ambedkar does not translate to physical harm. It is the intellectual capacity and desire for self-reflection that ruptures existing hierarchical power structures and renews the meaning of being human in democratic civilisations
The One Who Sees Me: Finding Hagar through Literary Hermeneutics and Religious Interpretive Agency
No full textThe figure of Hagar is a critical example of a marginalized woman of color whose actions and relationship to God models the course of theological liberation for the oppressed; but her story must first be her own. Examining the under-explored details of Hagar’s story and voice renders them (scripturally and diasporically) significant to broadly understanding the marginalized lives of women of color. Placing the Hagaritic findings and interpretive moves of Delores Williams in dialogue with Arabic, African, and Africandescended literature and religious scholarship allows for a broader understanding of Hagar’s experience to inform the lives of those who draw connection to her story