Revista de la Facultad de Ciencias Agrarias
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    621 research outputs found

    In vitro micropropagation and physiological assessment of Senecio bonariensis

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    Senecio bonariensis is a plant native to South American wetlands. This plant has ecological importance, is used in traditional medicine, and is also popular for ornamental purposes. This study aimed to develop the first in vitro propagation protocol for S. bonariensis. Leaf explants were disinfected and placed on Murashige and Skoog (MS) agar medium supplemented with different combinations of growth regulators. We tested the effect of two different cytokinins: Kinetin (KIN) and 6-benzylaminopurine (BAP), in the presence of the auxin α-naphthalene acetic acid (NAA). All treatments with KIN resulted in root production, but only treatments with BAP induced shoot formation. As results, we determined the optimal concentration for maximum shoot production, achieving a 100% success in rustication while finding similar physiological traits among micro-propagated and wild-type plants. In conclusion, we developed a protocol for the large-scale production of S. bonariensis plants, providing an alternative source of bioactive compounds for medical and pharmaceutical purposes while preserving the natural habitat of this native plant. Highlights: In Vitro Propagation Protocol: The study successfully developed the first in vitro propagation protocol for Senecio bonariensis, a plant native to South American wetlands. Growth Regulators Tested: The research explored the effects of two cytokinins, Kinetin (KIN) and 6-benzylaminopurine (BAP), alongside the auxin α-naphthalene acetic acid (NAA), on leaf explants. Shoot and Root Formation: While Kinetin induced root production in all treatments, only BAP was effective in promoting shoot formation, leading to the determination of optimal concentrations for maximum shoot production. Successful Rustication: The protocol achieved a 100% success rate in rustication, with micro-propagated plants displaying similar physiological traits to their wild-type counterparts. Applications and Conservation: The large-scale propagation protocol provides an alternative source of bioactive compounds for medical and pharmaceutical uses while helping to preserve the natural habitat of S. bonariensis. It also offers potential for applications in ecological studies, medicinal research, and commercial landscaping.Senecio bonariensis is a plant native to South American wetlands. This plant has ecological importance, is used in traditional medicine, and is also popular for ornamental purposes. This study aimed to develop the first in vitro propagation protocol for S. bonariensis. Leaf explants were disinfected and placed on Murashige and Skoog (MS) agar medium supplemented with different combinations of growth regulators. We tested the effect of two different cytokinins: Kinetin (KIN) and 6-benzylaminopurine (BAP), in the presence of the auxin α-naphthalene acetic acid (NAA). All treatments with KIN resulted in root production, but only treatments with BAP induced shoot formation. As results, we determined the optimal concentration for maximum shoot production, achieving a 100% success in rustication while finding similar physiological traits among micro-propagated and wild-type plants. In conclusion, we developed a protocol for the large-scale production of S. bonariensis plants, providing an alternative source of bioactive compounds for medical and pharmaceutical purposes while preserving the natural habitat of this native plant. Highlights: In Vitro Propagation Protocol: The study successfully developed the first in vitro propagation protocol for Senecio bonariensis, a plant native to South American wetlands. Growth Regulators Tested: The research explored the effects of two cytokinins, Kinetin (KIN) and 6-benzylaminopurine (BAP), alongside the auxin α-naphthalene acetic acid (NAA), on leaf explants. Shoot and Root Formation: While Kinetin induced root production in all treatments, only BAP was effective in promoting shoot formation, leading to the determination of optimal concentrations for maximum shoot production. Successful Rustication: The protocol achieved a 100% success rate in rustication, with micro-propagated plants displaying similar physiological traits to their wild-type counterparts. Applications and Conservation: The large-scale propagation protocol provides an alternative source of bioactive compounds for medical and pharmaceutical uses while helping to preserve the natural habitat of S. bonariensis. It also offers potential for applications in ecological studies, medicinal research, and commercial landscaping

    Morphostructural composition and meat quality in local goat kids from the northeastern region of Mexico

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    Goat farming is an important activity in northern Mexico. In this sense, “cabrito” or kid goat is a typical regional dish with high economic and cultural value. However, information on the morphostructural composition and meat quality of these local specimens is scarce. Given this, the objective was to evaluate morphostructural characteristics, carcass and meat quality in local kids according to sex in the northeastern region of Mexico. For this purpose, 14 kids (7 males and 7 females) 57 days old were slaughtered. Morphostructural composition was evaluated with 22 zoomometric and phenotypic variables. Carcass characteristics were evaluated by considering different body structures, carcass yield and degree of fatness. Meat quality was determined by physicochemical characteristics, nutritional value and fatty acid profile. The sex effect was evaluated by t-test of independent means and Chi-square. Meat physicochemical characteristics, nutritional value and morphostructure of local kids were heterogeneous and showed no differences (P≥0.05) concerning sex. Carcass, kidneys, head, neck, rib and loin weights were higher in males than in females (P≤0.05). Fatty acids (FA) found in greater proportion were palmitic (C16:0), oleic (C18:1, n-9), stearic (C18:0), and myristic (C14:0). These FA comprised 80.85 % of the lipid profile of male meat and 76.83% of females. These results are the basis for future programs aimed to improve production systems. Differences found could shed light on future efforts on how to differentiate goat meat from this region of Mexico and enter new markets directly benefiting small producers. Highlights: “Cabrito” or goat kid is a typical regional dish with high economic and cultural vale in north of México. Carcass structures like head, neck, rib and loin were higher in males. Fatty acids found in greater proportion in kid meat were palmitic (C16:0), oleic (C18:1, n-9), stearic (C18:0), and myristic. These fatty acids comprised 80.85% of lipid profile in males and 76.83% in females. Kid meat contains Docosahexaenoic acid (DHA) (C22: n-3), in quantities of 0.174 to 0.225 g/ 100 g of fat. This fatty acid is very important for brain development, as well as the cognitive and visual systems.Goat farming is an important activity in northern Mexico. In this sense, “cabrito” or kid goat is a typical regional dish with high economic and cultural value. However, information on the morphostructural composition and meat quality of these local specimens is scarce. Given this, the objective was to evaluate morphostructural characteristics, carcass and meat quality in local kids according to sex in the northeastern region of Mexico. For this purpose, 14 kids (7 males and 7 females) 57 days old were slaughtered. Morphostructural composition was evaluated with 22 zoomometric and phenotypic variables. Carcass characteristics were evaluated by considering different body structures, carcass yield and degree of fatness. Meat quality was determined by physicochemical characteristics, nutritional value and fatty acid profile. The sex effect was evaluated by t-test of independent means and Chi-square. Meat physicochemical characteristics, nutritional value and morphostructure of local kids were heterogeneous and showed no differences (P≥0.05) concerning sex. Carcass, kidneys, head, neck, rib and loin weights were higher in males than in females (P≤0.05). Fatty acids (FA) found in greater proportion were palmitic (C16:0), oleic (C18:1, n-9), stearic (C18:0), and myristic (C14:0). These FA comprised 80.85 % of the lipid profile of male meat and 76.83% of females. These results are the basis for future programs aimed to improve production systems. Differences found could shed light on future efforts on how to differentiate goat meat from this region of Mexico and enter new markets directly benefiting small producers. Highlights: “Cabrito” or goat kid is a typical regional dish with high economic and cultural vale in north of México. Carcass structures like head, neck, rib and loin were higher in males. Fatty acids found in greater proportion in kid meat were palmitic (C16:0), oleic (C18:1, n-9), stearic (C18:0), and myristic. These fatty acids comprised 80.85% of lipid profile in males and 76.83% in females. Kid meat contains Docosahexaenoic acid (DHA) (C22: n-3), in quantities of 0.174 to 0.225 g/ 100 g of fat. This fatty acid is very important for brain development, as well as the cognitive and visual systems

    Trends and research hotspots in principal genera of Platypodinae-fungi association: a bibliometric analysis on Euplatypus, Megaplatypus and Platypus (Coleoptera: Platypodinae)

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    Ambrosia beetles of the subfamily Platypodinae are symbiotically associated with fungi, which provide them with food and benefit their establishment and growth. In the present study, our interest centers on the principal genera of Platypodinae: Euplatypus, Megaplatypus and Platypus, the most relevant symbionts being species of Fusarium, Graphium and Raffaelea. The objective of this work is to update the description of fungal associations on those species of interest to the scientific community, ONGs and funding institutions. An exhaustive search was performed to cover all scientific studies from 1900 to 2024 on the co-occurrence or relationship between members of the above-mentioned Platypodinae and fungi. Records of insect and fungal species, host plants and geographic locations were collected. A bibliometric analysis was conducted to characterize the overall status, general trends and current research hotspots of fungi associated with these ambrosia beetles. Eighty percent of the publications retrieved explored the association of Platypus spp. with different fungi. Raffaelea was the fungal genus showing the highest number of records and worldwide distribution. Five countries from four continents currently lead research on these associations. However, greater insights into these interactions would improve decision-making on managing these pests. Highlights: Raffaelea was the most frequently recorded fungal genus associated with Platypodinae, with a worldwide distribution. Reports of Fusarium associated with Platypodinae are concentrated in the Northern Hemisphere, indicating its significance within forest health. Argentina, Japan, Portugal, South Korea, and USA, among others -alphabetically- are the countries with the most publications of associations fungi-Platypodinae.Ambrosia beetles of the subfamily Platypodinae are symbiotically associated with fungi, which provide them with food and benefit their establishment and growth. In the present study, our interest centers on the principal genera of Platypodinae: Euplatypus, Megaplatypus and Platypus, the most relevant symbionts being species of Fusarium, Graphium and Raffaelea. The objective of this work is to update the description of fungal associations on those species of interest to the scientific community, ONGs and funding institutions. An exhaustive search was performed to cover all scientific studies from 1900 to 2024 on the co-occurrence or relationship between members of the above-mentioned Platypodinae and fungi. Records of insect and fungal species, host plants and geographic locations were collected. A bibliometric analysis was conducted to characterize the overall status, general trends and current research hotspots of fungi associated with these ambrosia beetles. Eighty percent of the publications retrieved explored the association of Platypus spp. with different fungi. Raffaelea was the fungal genus showing the highest number of records and worldwide distribution. Five countries from four continents currently lead research on these associations. However, greater insights into these interactions would improve decision-making on managing these pests. Highlights: Raffaelea was the most frequently recorded fungal genus associated with Platypodinae, with a worldwide distribution. Reports of Fusarium associated with Platypodinae are concentrated in the Northern Hemisphere, indicating its significance within forest health. Argentina, Japan, Portugal, South Korea, and USA, among others -alphabetically- are the countries with the most publications of associations fungi-Platypodinae

    First report of the causal agent of vine crown gall in Mendoza, Argentina

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    Crown gall is one widespread grapevine disease worldwide, caused by Allorhizobium vitis (syn. Agrobacterium vitis) and Agrobacterium tumefaciens (syn. Rhizobium radiobacter). All. Vitis, is the predominant species and primary cause of the disease. This study aimed to identify and characterize molecularly the agrobacteria in plants with crown gall symptoms in Mendoza vineyards. Diseased plants with trunk-based galls were sampled from different areas of Mendoza. Two multiplex PCRs were performed for bacterial identification and characterization, demonstrating that 91.6% of the strains obtained were agrobacteria (77% A. tumefaciens and 23% All. vitis). Fifty percent of All. vitis and 16% of A. tumefaciens identified strains were pathogenic. Pathogenicity tests were also conducted on Kalanchoe daigremontiana, with resulting tumorigenic symptoms. Highlights: Crown gall is one widespread grapevine disease in the world, caused by Allorhizobium vitis (syn. Agrobacterium vitis) and Agrobacterium tumefaciens (syn. Rhizobium radiobacter). This study aimed to identify and characterize molecularly the causal agent of crown gall in Mendoza vineyards. One hundred and forty-eight symptomatic plants were collected from various vine-growing areas of Mendoza. Allorhizobium vitis and Agrobacterium tumefaciens strains were molecularly identified as pathogenic and octopine/nopaline type. All vitis is the predominant pathogenic species in grapevines studied in Mendoza.Crown gall is one widespread grapevine disease worldwide, caused by Allorhizobium vitis (syn. Agrobacterium vitis) and Agrobacterium tumefaciens (syn. Rhizobium radiobacter). All. Vitis, is the predominant species and primary cause of the disease. This study aimed to identify and characterize molecularly the agrobacteria in plants with crown gall symptoms in Mendoza vineyards. Diseased plants with trunk-based galls were sampled from different areas of Mendoza. Two multiplex PCRs were performed for bacterial identification and characterization, demonstrating that 91.6% of the strains obtained were agrobacteria (77% A. tumefaciens and 23% All. vitis). Fifty percent of All. vitis and 16% of A. tumefaciens identified strains were pathogenic. Pathogenicity tests were also conducted on Kalanchoe daigremontiana, with resulting tumorigenic symptoms. Highlights: Crown gall is one widespread grapevine disease in the world, caused by Allorhizobium vitis (syn. Agrobacterium vitis) and Agrobacterium tumefaciens (syn. Rhizobium radiobacter). This study aimed to identify and characterize molecularly the causal agent of crown gall in Mendoza vineyards. One hundred and forty-eight symptomatic plants were collected from various vine-growing areas of Mendoza. Allorhizobium vitis and Agrobacterium tumefaciens strains were molecularly identified as pathogenic and octopine/nopaline type. All vitis is the predominant pathogenic species in grapevines studied in Mendoza

    Selection of Rhizobium leguminosarum strains via symbiotic and production variables in Pisum sativum L.

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    Field pea (Pisum sativum L.) is a winter symbiotic legume that associates with Rhizobium leguminosarum sv viciae. This work aimed to evaluate strains of R. leguminosarum for their infective ability and early-plant growth, BNF contribution, biomass and grain yield. Seventy-eight specific strains and four pea cultivars were evaluated in a growth chamber, five strains and three cultivars were evaluated in a greenhouse, and three strains and two cultivars were evaluated in a field experiment. Only 44-55% of all evaluated strains were infective in the four cultivars. In the greenhouse, D70 and D156 strains showed the best nodulation variables as well as higher N content and yield. The field experiment showed D156 and D70 yielded a similar behavior for N content in canopy biomass and individual nodule biomass, whereas D191 had a higher nodule number per plant, aerial biomass and grain yields. D70 provided good nodulation, N content in biomass, and yield in the growth chamber, greenhouse, and field experiments, whereas D156 had a like or superior behavior in the greenhouse and field experiments. Therefore, D156 could constitute a good candidate for bacterial single-strain inoculants, as well as for formulating microbial consortia. Highlights: Only 44-55% of all the strains tested presented nodules in the four commercial cultivars. The isolates from the genus Lathyrus had a higher proportion of infective and effective nodules (60%), whereas the isolates from Pisum had only 8%. The D156 strain exhibited similar or better behavior than the reference strain D70 in chamber growth, greenhouse, and field experiments. The D156 strain could be a good candidate for bacterial single-strain inoculants, as well as for a microbial consortium with D70 for Pisum sativum (L).Field pea (Pisum sativum L.) is a winter symbiotic legume that associates with Rhizobium leguminosarum sv viciae. This work aimed to evaluate strains of R. leguminosarum for their infective ability and early-plant growth, BNF contribution, biomass and grain yield. Seventy-eight specific strains and four pea cultivars were evaluated in a growth chamber, five strains and three cultivars were evaluated in a greenhouse, and three strains and two cultivars were evaluated in a field experiment. Only 44-55% of all evaluated strains were infective in the four cultivars. In the greenhouse, D70 and D156 strains showed the best nodulation variables as well as higher N content and yield. The field experiment showed D156 and D70 yielded a similar behavior for N content in canopy biomass and individual nodule biomass, whereas D191 had a higher nodule number per plant, aerial biomass and grain yields. D70 provided good nodulation, N content in biomass, and yield in the growth chamber, greenhouse, and field experiments, whereas D156 had a like or superior behavior in the greenhouse and field experiments. Therefore, D156 could constitute a good candidate for bacterial single-strain inoculants, as well as for formulating microbial consortia. Highlights: Only 44-55% of all the strains tested presented nodules in the four commercial cultivars. The isolates from the genus Lathyrus had a higher proportion of infective and effective nodules (60%), whereas the isolates from Pisum had only 8%. The D156 strain exhibited similar or better behavior than the reference strain D70 in chamber growth, greenhouse, and field experiments. The D156 strain could be a good candidate for bacterial single-strain inoculants, as well as for a microbial consortium with D70 for Pisum sativum (L)

    Traditional cow-calf systems of the northern region of Santa Fe, Argentina: current situation and improvement opportunities

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    Cow-calf systems are at the core of Argentina´s significant national beef industry. The objectives were: i) to characterize the productive state of traditional cow-calf systems, named BASE, from the northern region of Santa Fe province, ii) to identify technologies for the productive improvement of the BASE system, and iii) to quantify the productive and economic impact of the adoption of the identified technologies. To characterize the BASE system, the available published data were systematized and validated in a workshop with leading regional experts in the field. To identify the technologies for improvement, a survey was conducted among regional farm advisors. Finally, to quantify the impact of adopting improvements in the BASE system, a modelling study was conducted. The results showed that traditional cow-calf systems have low productive and reproductive efficiency (45 kg LW ha-1 year-1 and 48% weaning rate) and little adoption of herd management and forage production technologies. The technologies identified were grazing management, training of farmers and farm staff, and seasonal mating. The modelling study showed that improvements in the production and use of forage and herd management practices would increase beef production and the gross margin of the BASE system by 70% and 96%, respectively. Highlights: Participatory modelling was used to characterize and evaluate the traditional cow-calf system from northern region of Santa Fe province and prioritize improvement technologies for potential adoption. Cow-calf systems from the northern region of Santa Fe province have little adoption of technologies and low productive and reproductive efficiency (45 kg LW ha-1 year-1 and 48% weaning rate). Technologies prioritized by regional farm advisors of cow-calf systems were grazing management, training of farmers and farm staff, and seasonal mating. The combination of herd and feeding strategies would increase beef production and gross margin of traditional cow-calf system by up to 70% and 96%, respectively.Cow-calf systems are at the core of Argentina´s significant national beef industry. The objectives were: i) to characterize the productive state of traditional cow-calf systems, named BASE, from the northern region of Santa Fe province, ii) to identify technologies for the productive improvement of the BASE system, and iii) to quantify the productive and economic impact of the adoption of the identified technologies. To characterize the BASE system, the available published data were systematized and validated in a workshop with leading regional experts in the field. To identify the technologies for improvement, a survey was conducted among regional farm advisors. Finally, to quantify the impact of adopting improvements in the BASE system, a modelling study was conducted. The results showed that traditional cow-calf systems have low productive and reproductive efficiency (45 kg LW ha-1 year-1 and 48% weaning rate) and little adoption of herd management and forage production technologies. The technologies identified were grazing management, training of farmers and farm staff, and seasonal mating. The modelling study showed that improvements in the production and use of forage and herd management practices would increase beef production and the gross margin of the BASE system by 70% and 96%, respectively. Highlights: Participatory modelling was used to characterize and evaluate the traditional cow-calf system from northern region of Santa Fe province and prioritize improvement technologies for potential adoption. Cow-calf systems from the northern region of Santa Fe province have little adoption of technologies and low productive and reproductive efficiency (45 kg LW ha-1 year-1 and 48% weaning rate). Technologies prioritized by regional farm advisors of cow-calf systems were grazing management, training of farmers and farm staff, and seasonal mating. The combination of herd and feeding strategies would increase beef production and gross margin of traditional cow-calf system by up to 70% and 96%, respectively

    Food labeling in Argentina. Decoding impacts on the Argentine Food Code

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    This work aims to analyze the characteristics of the legislative codification technique in Argentina, and whether, since the enactment of the Food Code to the present, this technique has been affected by a decoding normative evolution, considering the food labeling regime a particular study case. As a result, three lines of legislative alteration or modification distorting the mentioned technique re identified. This generates inconsistencies and ambiguities in the legislation, and consequent negative effects on the application, compliance and understanding of food regulations in the industry. Highlights: The Argentine Food Regime was based on the legislative technique of codification. It can be stated that the Argentine Food Code has legislative action lines in tension with the codification legislative technique. Since the enactment the Food Code there has been a decoding evolution, with negative effects on the application, compliance and understanding of the regulations in the food industry.This work aims to analyze the characteristics of the legislative codification technique in Argentina, and whether, since the enactment of the Food Code to the present, this technique has been affected by a decoding normative evolution, considering the food labeling regime a particular study case. As a result, three lines of legislative alteration or modification distorting the mentioned technique re identified. This generates inconsistencies and ambiguities in the legislation, and consequent negative effects on the application, compliance and understanding of food regulations in the industry. Highlights: The Argentine Food Regime was based on the legislative technique of codification. It can be stated that the Argentine Food Code has legislative action lines in tension with the codification legislative technique. Since the enactment the Food Code there has been a decoding evolution, with negative effects on the application, compliance and understanding of the regulations in the food industry

    Green manuring and fertilization on rice (Oryza sativa L.): a peruvian Amazon study

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    The study was conducted in Juan Guerra district, province and region of San Martin, Peru; it assessed two treatment sets: (1) nitrogen fertilizer dose (FN75, FN100); (2) green manure Crotalaria juncea (CroJ), Canavalia ensiformis (CanE), and without green manure. It was arranged in a split-plot design with four replications. During the experiment, we observed an important fluctuation in soil parameters. Notably, there was a decrease in soil carbon and nitrogen levels, likely attributed to microorganism metabolism. On the other hand, we observed that CanE significantly reduced the diseased tillers through “White Leaf Virus” (RHBV) by 2.82% compared to the control, and significant panicle fertility was achieved by CroJ (91.88%). No significant differences were obtained in yields during this first campaign; however, the highest reported yield was 8.36 t ha-1 with the CanE - FN100 treatment. Additionally, the nutritional quality of the rice was not affected by either green manuring or the application of chemical nitrogen fertilization. These findings allow deeper studies to consider strategic alternatives to reducing dependency on inorganic fertilizers among the poorest communities. Highlights: A reduced incidence of White Leaf Virus (RHBV) was observed with the use of green manures. Rice variety INIA 507 "La Conquista'' reached 8.36 t ha-1 under tropical climate conditions. Canavalia ensiformis accompanied by nitrogen fertilization produced the highest yield. Replacing 25% of chemical nitrogen fertilization with Canavalia or Crotalaria green manures did not significantly affect rice yield. Replacing chemical nitrogen fertilization with green manure did not affect the nutritional quality of rice.The study was conducted in Juan Guerra district, province and region of San Martin, Peru; it assessed two treatment sets: (1) nitrogen fertilizer dose (FN75, FN100); (2) green manure Crotalaria juncea (CroJ), Canavalia ensiformis (CanE), and without green manure. It was arranged in a split-plot design with four replications. During the experiment, we observed an important fluctuation in soil parameters. Notably, there was a decrease in soil carbon and nitrogen levels, likely attributed to microorganism metabolism. On the other hand, we observed that CanE significantly reduced the diseased tillers through “White Leaf Virus” (RHBV) by 2.82% compared to the control, and significant panicle fertility was achieved by CroJ (91.88%). No significant differences were obtained in yields during this first campaign; however, the highest reported yield was 8.36 t ha-1 with the CanE - FN100 treatment. Additionally, the nutritional quality of the rice was not affected by either green manuring or the application of chemical nitrogen fertilization. These findings allow deeper studies to consider strategic alternatives to reducing dependency on inorganic fertilizers among the poorest communities. Highlights: A reduced incidence of White Leaf Virus (RHBV) was observed with the use of green manures. Rice variety INIA 507 "La Conquista'' reached 8.36 t ha-1 under tropical climate conditions. Canavalia ensiformis accompanied by nitrogen fertilization produced the highest yield. Replacing 25% of chemical nitrogen fertilization with Canavalia or Crotalaria green manures did not significantly affect rice yield. Replacing chemical nitrogen fertilization with green manure did not affect the nutritional quality of rice

    Nutritional quality of amaranth (Amaranthus) silage in response to forage airing and addition of lactic bacteria

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    Climate change is reducing forage availability for ruminants. Previous studies in Northern Patagonia, Argentina, have demonstrated the adaptation of the amaranth crop to these agroclimatic conditions under irrigation. Moreover, this crop is used as forage in marginal areas of the world, given its outstanding productive and nutritional qualities. The objective of this study was to evaluate the nutritional quality of amaranth silage in response to previous wilting and the addition of lactic acid bacteria. The crop was harvested at the milky grain stage and ensiled in experimental microsilos for 60 days. Before ensiling, different treatments (wilting and addition of lactic acid bacteria) were applied. Parameters related to nutritional quality were evaluated, including ash, crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), dry matter digestibility (DMD), and metabolizable energy (ME). Simultaneous treatment with air and the addition of lactic acid bacteria before ensiling resulted in the best nutritional quality  characteristics of the silage. The most significant results were protein value of 12.7%, 41.1% NDF and 19.1% FDA. The DM and ME were 74% and 2.67 Mcal/kg, respectively. Thus, amaranth silage can be considered an alternative conserved forage for animal feed in this region. Highlights: Amaranth crops as a resilient forage option for regions facing climatic challenges such as northern Patagonia, Argentina. The dry matter reduction of the ensiled plant material was <1% and the high protein content of amaranth did not affect the ensiling process. Simultaneous treatment such as wilting and addition of lactic acid bacteria to the plant material prior to ensiling resulted in the best nutritional quality characteristics of the silage obtained. A synergistic effect was observed among the treatments evaluated, achieving a silage with a digestibility of 74.02% comparable to that of corn silage.Climate change is reducing forage availability for ruminants. Previous studies in Northern Patagonia, Argentina, have demonstrated the adaptation of the amaranth crop to these agroclimatic conditions under irrigation. Moreover, this crop is used as forage in marginal areas of the world, given its outstanding productive and nutritional qualities. The objective of this study was to evaluate the nutritional quality of amaranth silage in response to previous wilting and the addition of lactic acid bacteria. The crop was harvested at the milky grain stage and ensiled in experimental microsilos for 60 days. Before ensiling, different treatments (wilting and addition of lactic acid bacteria) were applied. Parameters related to nutritional quality were evaluated, including ash, crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), dry matter digestibility (DMD), and metabolizable energy (ME). Simultaneous treatment with air and the addition of lactic acid bacteria before ensiling resulted in the best nutritional quality  characteristics of the silage. The most significant results were protein value of 12.7%, 41.1% NDF and 19.1% FDA. The DM and ME were 74% and 2.67 Mcal/kg, respectively. Thus, amaranth silage can be considered an alternative conserved forage for animal feed in this region. Highlights: Amaranth crops as a resilient forage option for regions facing climatic challenges such as northern Patagonia, Argentina. The dry matter reduction of the ensiled plant material was <1% and the high protein content of amaranth did not affect the ensiling process. Simultaneous treatment such as wilting and addition of lactic acid bacteria to the plant material prior to ensiling resulted in the best nutritional quality characteristics of the silage obtained. A synergistic effect was observed among the treatments evaluated, achieving a silage with a digestibility of 74.02% comparable to that of corn silage

    Pseudocereals dietary fiber. Amaranth, quinoa, and buckwheat fiber composition and potential prebiotic effect

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    Amaranth (Amaranthus), buckwheat (Fagopyrum esculentum), and quinoa (Chenopodium quinoa) crops have limited production and agro-industrial development both in Argentina and globally. As the demand for functional ingredients and foods grows, developing products from these pseudocereals could offer substantial economic benefits. This study aims to analyze the dietary fiber content and composition of amaranth, quinoa, and buckwheat, and to investigate the relationship between dietary fiber structure and its potential prebiotic effects. Gaining insights into these aspects would provide valuable information for developing foods based on these pseudocereals and could enhance their future applications in the food industry. Highlights: Pseudocereals have TDF levels similar to wheat, with varying fiber compositions. Amaranth and quinoa have high IDF/SDF ratios, close to the ideal 3:1 balance. Studies reveal pseudocereal fiber boosts beneficial microbes and SCFA production. Pseudocereals offer evidenced prebiotic benefits but need more research for validation. Amaranth, quinoa, and buckwheat are promising functional food ingredients.Amaranth (Amaranthus), buckwheat (Fagopyrum esculentum), and quinoa (Chenopodium quinoa) crops have limited production and agro-industrial development both in Argentina and globally. As the demand for functional ingredients and foods grows, developing products from these pseudocereals could offer substantial economic benefits. This study aims to analyze the dietary fiber content and composition of amaranth, quinoa, and buckwheat, and to investigate the relationship between dietary fiber structure and its potential prebiotic effects. Gaining insights into these aspects would provide valuable information for developing foods based on these pseudocereals and could enhance their future applications in the food industry. Highlights: Pseudocereals have TDF levels similar to wheat, with varying fiber compositions. Amaranth and quinoa have high IDF/SDF ratios, close to the ideal 3:1 balance. Studies reveal pseudocereal fiber boosts beneficial microbes and SCFA production. Pseudocereals offer evidenced prebiotic benefits but need more research for validation. Amaranth, quinoa, and buckwheat are promising functional food ingredients

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