OHSU Digital Collections (Oregon Health and Science University)
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Immunopathogenesis of emerging arboviral infections and their impact on maternal–fetal health
No full textThis dissertation uses nonhuman primate models to define how emerging arboviruses disrupt placental function and threaten fetal health. Through comparative studies of Zika virus strains and evaluation of a virus-like particle vaccine, this work identifies key mechanisms underlying congenital disease and pregnancy loss. The development of a contemporary O’nyong-nyong virus model further expands these findings to support vaccine development and epidemic preparedness
From method to biological discovery: decoding cancer heterogeneity
No full textThis dissertation presents the development of an improved approach for single-cell whole genome sequencing that achieves the quantitative metrics of gold-standard assays—including uniform, high genome coverage and high cell throughput—while relying solely on commercially available reagents, chemistry, and instrumentation
Photocatalyst mediated subcellular protein compartment lableling
No full textCellular function relies on the spatial organization of proteins, and shifts in localization patterns are hallmarks of diseases. Modern proteomics is steadily advancing efforts to identify proteomes with their spatial contexts to understand disease onset and progression. Microscopy provides high-resolution insights into protein localizations, and mass spectrometry delivers wider coverage of the proteome. The integration of optical precision with mass spectrometry is thus crucial for uncovering how spatial localizations drive diseases or therapeutic response. In this review, we highlight cutting-edge photo- proteomic approaches such as μMap, STOMP, and optoproteomics that integrate photolabeling with proteome discovery to achieve a nanometer-scale interactome. These tools now enable high-resolution mapping of proteome organization and open new directions in in vitro, in vivo, FFPE tissue, and computationally integrated spatial proteomics. Developments in photocatalyst chemistry, automated photolabeling workflows, and computational frameworks for integrating imaging and proteomic data from cultured cells to complex tissues pave the way to transform spatial proteomics into a powerful platform for detecting disease and its trajectory
Documenting the discussion: improving shared decision-making through standardized documentation
No full textThis quality improvement initiative aimed to enhance the documentation of shared decision-making (SDM) in a faculty nurse-midwifery practice through the implementation of a standardized template. Over three Plan-Do-Study-Act (PDSA) cycles, midwives reported greater satisfaction (p = 0.035) and efficiency (p = 0.008) with the structured template compared to prior documentation methods, as well as improved ability to track SDM conversations over time (p = 0.003). While adoption varied, the findings highlight the potential for standardized SDM documentation to improve continuity of care without increasing workload, though better EHR integration, training, and workflow alignment may enhance usability and consistency
Identifying phenotype associated cell-cell co-occurrence by integrate single cell RNA-seq data and bulk RNA-seq data
No full textCell-cell co-occurrence patterns are critical in shaping biological and clinical phenotypes, but existing tools for analyzing single-cell RNA sequencing (scRNA-seq) data often lack phenotype annotations and face scalability limitations. To address this, we developed iPhenoChat, a cluster-free computational method that integrates bulk and single-cell RNA-seq data to identify phenotype-associated cell-cell interactions. Leveraging machine learning and optimized for speed using PyTorch and GPU acceleration, iPhenoChat bridges single-cell resolution with bulk-level phenotyping, enabling deeper insights into disease progression and clinical outcomes
Improving access to cervical cancer screening among Somali Refugees
No full textThis DNP project aimed to promote awareness and knowledge of CCS and its prevention through a culturally tailored educational workshop for Somali women in the Portland metropolitan area. Using the CBPR framework, the project emphasizes collaboration with community leaders and organizations to ensure cultural relevance, build trust, and address systemic barriers