Saitama Medical University Repository
Not a member yet
720 research outputs found
Sort by
A highly specific and sensitive massive parallel sequencer-based test for somatic mutations in non-small cell lung cancer.
Get PDF埼玉医科大学博士（医学）平成29年度Molecular targeting therapy for non-small cell lung cancer (NSCLC) has clarified the importance of mutation testing when selecting treatment regimens. As a result, multiple-gene mutation tests are urgently needed. We developed a next-generation sequencer (NGS)-based, multi-gene test named the MINtS for investigating driver mutations in both cytological specimens and snap-frozen tissue samples. The MINtS was used to investigate the EGFR, KRAS, BRAF genes from DNA, and the ERBB2, and the ALK, ROS1, and RET fusion genes from RNA. We focused on high specificity and sensitivity (≥0.99) and even included samples with a cancer cell content of 1%. The MINtS enables testing of more than 100 samples in a single run, making it possible to process a large number of samples submitted to a central laboratory, and reducing the cost for a single sample. We investigated 96 cytological samples and 190 surgically resected tissues, both of which are isolated in daily clinical practice. With the cytological samples, we compared the results for the EGFR mutation between the MINtS and the PNA-LNA PCR clamp test, and their results were 99% consistent. In the snap-frozen tissue samples, 188/190 (99%) samples were successfully analyzed for all genes investigated using both DNA and RNA. Then, we used 200 cytological samples that were serially isolated in clinical practice to assess RNA quality. Using our procedure, 196 samples (98%) provided high-quality RNA suitable for analysis with the MINtS. We concluded that the MINtS test system is feasible for analyzing "druggable" genes using cytological samples and snap-frozen tissue samples. The MINtS will fill a needs for patients for whom only cytological specimens are available for genetic testing
Grading system for blood vessel tumor emboli of invasive ductal carcinoma of the breast
No full text埼玉医科大学博士（医学）平成28年
Diagnosis and potential management of gestational diabetes mellitus using the International Association of Diabetes and Pregnancy Study Groups criteria
No full text埼玉医科大学博士（医学）平成28年
Correlation Between Tumor Mesothelin Expression and Serum Mesothelin in Patients with Epithelial Ovarian Carcinoma: A Potential Noninvasive Biomarker for Mesothelin-targeted Therapy
No full text埼玉医科大学博士（医学）平成28年
Placental (pro)renin receptor expression and plasma soluble (pro)renin receptor levels in preeclampsia
No full text埼玉医科大学博士（医学）平成28年
