eData: the STFC Research Data Repository
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SMLM Data from "Reinforced optical cage systems enable drift-free single-molecule localization microscopy"
No full textThis dataset contains:
1) Five image series' of fluorescent beads and their subpixel localisations in each frame, acquired with a homebuilt microscope. .tif files for images, .csv files for localization data
2) Five image series' of fluorescent beads and their subpixel localisations in each frame, acquired with a commercial microscope. .czi files for images, .csv files for localization data
3) STORM image series of HeLa cells with alpha-tubulin immunolabelled with antibodies conjugated to Alexa Fluor 647 and localisation data, acquired with a homebuilt microscope. .tif files for images, .csv files for localization data
4) STORM image series of HeLa cells with alpha-tubulin immunolabelled with antibodies conjugated to Alexa Fluor 647 and localisation data, acquired with a commercial microscope. .czi files for images, .csv files for localization data
5) STORM image series of U2OS cells with Nup96-SNAP-AF647 and localisation data acquired with a homebuilt microscope. tif files for images, .csv files for localization data
6) STORM image series of U2OS cells with Nup96-SNAP-AF647 and localisation data acquired with a commercial microscope. .czi files for images, .csv files for localization dataThe Stoneygate Trust Seedcorn2022\100230, STFC Proof of Concept Fund: POCF2021-14, STFC Cancer Diagnosis Network+ ST/S005404/1, BBSRC BB/T008784/
Additional SMLM Data from "Reinforced optical cage systems enable drift-free single-molecule localization microscopy"
No full textThis dataset contains additional data for the paper titled 'Reinforced optical cage systems enable drift-free single-molecule localization microscopy' that has been generated to address reviewer comments. The dataset has the following:
1. Five image series' of fluorescent beads and their subpixel localisations in each frame, acquired with a homebuilt microscope. .tif files for images, .csv files for localization data. Used for axial drift and lateral drift quantification.
2. Five image series' of fluorescent beads and their subpixel localisations in each frame, acquired with a commercial microscope. .tif files for images, .csv files for localization data
3. STORM image series of HeLa cells with alpha-tubulin immunolabelled with antibodies conjugated to Alexa Fluor 647 acquired with a homebuilt microscope
4. STORM image series of HeLa cells with alpha-tubulin immunolabelled with antibodies conjugated to Alexa Fluor 647 acquired with a commercial microscope.
5. STORM image series of U2OS cells with Nup96-SNAP-AF647 and localisation data acquired with a homebuilt microscope.
6. STORM image series of U2OS cells with Nup96-SNAP-AF647 and localisation data acquired with a commercial microscope
STL file and evaluation data sets for "Additive manufacturing in aluminium of a primary mirror for a cubesat application: manufacture, testing and evaluation"
No full textCAD files, engineering drawings and evaluation data sets for "Additive manufacturing in aluminium of a primary mirror for a cubesat application: manufacture, testing and evaluation".
stl file of the final as-printed part as well as the as-printed and as-machined (post SPDT) engineering drawings
XCT scan tif and xtekc files (alternative file types are available upon request)
High resolution jpg and tif files of the images used in the reportMR/T042230/
Scanning electron microscopy images and energy dispersive X-ray spectroscopy of inclusions within AlSi10Mg mirrors created using additive manufacturing
No full textThe data provided herein describes the evaluation of inclusions within the surfaces of six aluminium mirrors created using additive manufacturing in the alloy AlSi10Mg. The inclusions are frequently observed to cause scratches on the mirror surface and by identification, routes for mitigation can be provided. The inclusions were evaluated using scanning electron microscopy and energy dispersive X-ray spectroscopy and subsequently were observed to be either aluminium oxides or contaminating metal alloys. The mirrors utilised different additive manufacturing machines with different operation environments, which allow the data to inform on routes where contamination has occurred.
The data are organised by sample (mirror) number and the evaluated inclusion, with individual files describing topography and spectroscopy. The mirrors were approx. 50 mm in diameter and between 5 and 10 mm in height. The equipment used was a Zeiss Crossbeam 550 FIB-SEM located at the University of Edinburgh. Five detectors on the FIB-SEM were used in data collection: InLens, SESI, ESD, BSD4 and EDS.MR/T042230/
Data associated with manuscript "Demonstration of stable, long-term operation of a nanosecond pulsed DPSSL operating at 10 J, 100 Hz"
No full textData on the characterisation of DiPOLE 10 J, 100 Hz laser system.European Regional Development Fund (CZ.02.1.01/0.0/0.0/15 006/0000674);
Horizon 2020 Framework Programme (739573);
Engineering \& Physical Sciences Research Council (EPSRC) grants EP/L01596X/1 and EP/S022821/1
Characterisation and mitigation of RF knockout during beam stacking - ISIS experimental data
No full textData used in the paper titled - Characterisation and mitigation of RF knockout during beam stacking
The data consists of .tek and .npz binary files. The files have data keys "time" and "data".
The data can be read into Python using the example script provided.
The spreadsheet provides guide to each data file and its contents as well the relevant figure in the paper
Residual SORS spectra of Historical Wet Collections
No full textRaman spectra discussed in the pubblication "Characterizing Noninvasively Conservation Status of Historical Wet Collections using Spatially Offset Raman Spectroscopy".
The dataset consist in 3 different repetition for each sample. Data procesing is explained in detail in the linked paper. Briefly, the glass/container contribution (zero-displacement spectrum) was subtracted from the corresponding offset spectrum to isolate the preservation fluid signature (SORS spectrum). Each SORS spectrum was then compared to the appropriate reference standard fluid (e.g., ethanol 50%–70%, formaldehyde 4%–5%, see Table 1), and subtraction of the reference spectrum was performed to enhance minor spectral contributions. The so called "Residual" Raman spectra are here attached for the four example of historic samples discussed in the paper: (E1) a sample collected by Charles Darwin during the second voyage of HMS Beagle (1831–1836); (E2) a mid-20th century specimen from the former Wellcome Trust collection; (E3) a mixed fish sample from the museum’s “Tank Room,” from the late 20th century; and (E4) experimental preservation material of uncertain provenance and composition, dating from the early- to mid-20th century
Data for: The use of FLIM for characterising chromosomes and their structure in response to low-dose X-ray irradiation
No full textThe files in this repository are the raw and analysed data presented in the paper titled above. The experimental conditions are highlighted in the following published articles. We applied these methods to different DNA stains bound to chromosomes where only DAPI led to a clear lifetime difference between the chromosome arms (p,q). Our data showed that chromosomes of cells irradiated with 0.1 Gy and 1 Gy did not show a significant change in lifetimes. These findings indicate that DAPI FLT may be a useful tool to measure chromosomal structural changes and further suggests that chromosomes undergo distinct structural changes at the pericentromeric region following low-dose irradiation.
Note: this deposit is continued by: https://doi.org/10.5286/edata/962BBSRC grant BB/H022597/
Simulation study of suprathermal ion fusion in high density deuterium
No full textAll of the simulation data supporting (input deck, cross-section files, outputs) supporting PPCF-105027.R1. Code used was the SPINOSUS code.
run2 : contains baseline run
setA...setE correspond to the runs referred to in the manuscript textn/
Dataset for the experiment OMEAG_SAI-23A, written up in manuscript "Performance Enhancement of Direct-Drive Shock Augmented Ignition Inertial Fusion Implosions through Shock Timing Optimisation"
No full textData taken during the OMEAG_SAI-23A "Shock Augmented Ignition" shot day. Investigated the novel scheme of Shock Augmented Ignition (SAI) [10.1103/PhysRevLett.129.195001] for Inertial Confinement Fusion (ICF) [10.1063/1.4934714]. Data from multiple diagnostics were taken. Diagnostics include neutron, proton, and x-ray imagers. All were used to assess the performance of the implosion and the dependency on the timing of the central "augmenting shock".EPSRC grants No. EP/P023460/1, No. EP/P026796/1 and EP/L01663X/1.
Department of Energy [National Nuclear Security Administration] University of Rochester ``National Inertial Confinement Fusion Program" under Award Number DE-NA0004144