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    Staphylococcus species involved in pet abscesses diseases

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    Current research is important due to the frequency of cases caused by some pathologies of abscesses recorded in pets. During these researches, microbiological analyzes were taken from various abscesses of canines and cats by assessing the virulence and prevalence of the identified bacterial microflora species. Microbiological laboratory investigations of abscesses of different origins revealed the presence of a bacterial microflora consisting of the prevalent species Staphylococcus aureus and Staphylococcus epidermidis in all investigated samples. S.aureus and S.epidermidis strains isolated from various areas of body pathological abscesses in pets revealed significant bacterioscopic and bacteriological microbiological values in the abscesses of the mandible, paw, ears and oral cavity demonstrating phenotypic mechanisms of aggression

    Endocannabinoid Modulation in Neurodegenerative Diseases: In Pursuit of Certainty

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    Neurodegenerative diseases are an increasing cause of global morbidity and mortality. They occur in the central nervous system (CNS) and lead to functional and mental impairment due to loss of neurons. Recent evidence highlights the link between neurodegenerative and inflammatory diseases of the CNS. These are typically associated with several neurological disorders. These diseases have fundamental differences regarding their underlying physiology and clinical manifestations, although there are aspects that overlap. The endocannabinoid system (ECS) is comprised of receptors (type-1 (CB1R) and type-2 (CB2R) cannabinoid-receptors, as well as transient receptor potential vanilloid 1 (TRPV1)), endogenous ligands and enzymes that synthesize and degrade endocannabinoids (ECBs). Recent studies revealed the involvement of the ECS in different pathological aspects of these neurodegenerative disorders. The present review will explore the roles of cannabinoid receptors (CBRs) and pharmacological agents that modulate CBRs or ECS activity with reference to Alzheimer’s Disease (AD), Parkinson’s Disease (PD), Huntington’s Disease (HD) and multiple sclerosis (MS)

    Diversification as an alternative strategy for sustainable farm development

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    Structural changes in agriculture have drawn attention to new opportunities to use the farm's resources. In order to support rural development, the agricultural policy makers have consistently promoted multifunctionality and diversification of farms by encouraging new types of activities. Farmers get involved in diversification operations and developed on-farm and off-farm activities for various reasons: to face the challenges of the agricultural context, to obtain additional income, continuity of their agricultural activity, to improve their family's quality of life and to reduce the risk of the unstable agricultural market environment. The decision regarding farm diversification depends on a series of factors related to economic, geophysical and socio-demographic farm characteristics or to environmental conservation strategies. The proximity to urban areas fosters the process of farm diversification and it also influences the level of diversification. The sustainability of the farm involves not only economic, but also social and environmental aspects, but most of the farmers are more interested in the economic and social performance and less in the ecological performance. The main forms of farm diversification include: agritourism; non-traditional crops, livestock and practices; added value to existing agricultural products; new marketing and distribution channels; consulting or education service; conservation and restoration of historical buildings, equipment, artifacts from the farm

    Medicinal plant growth in heavy metals contaminated soils: responses to metal stress and induced risks to human health

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    Accelerating heavy metal pollution is a hot issue due to a continuous growth in consumerism and increased activities in various global industries. Soil contamination with heavy metals has resulted in their incorporation into the human food web via plant components. Accumulation and amplification of heavy metals in human tissues through the consumption of medicinal plants can have hazardous health outcomes. Therefore, in this critical review we aim to bring together published information on this subject, with a special highlight on the knowledge gaps related to heavy metal stress in medicinal plants, their responses, and human health related risks. In this respect, this review outlines the key contamination sources of heavy metals in plants, as well as the absorption, mobilization and translocation of metal ions in plant compartments, while considering their respective mechanisms of detoxification. In addition, this literature review attempts to highlight how stress and defensive strategies operate in plants, pointing out the main stressors, either biotic or abiotic (e.g., heavy metals), and the role of reactive oxygen species (ROS) in stress answers. Finally, in our research, we further aim to capture the risks caused by heavy metals in medicinal plants to human health through the assessment of both a hazard quotient (HQ) and hazard index (HI)

    Effects of hybrid polymeric material based on polycaprolactone on the environment

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    Polymers are of great interest in areas such as agriculture, medicine and pharmacy, the food and cosmetic industries, and the chemical and construction industries. However, many polymers are nonbiodegradable and are not environmentally friendly. They are highly resistant to degradation and therefore can lead to waste disposal problems. In recent years, the interest in the microbial degradation of polymeric materials has grown due to the desire for less waste pollution in the environment. In this study, the biodegradable polymer that was obtained by the ring-opening polymerization of "-caprolactone (CL) using an aminopropyl-polydimethylsiloxane (APDMS) oligomer and the effects of the polymer towards the growth and development of tomato plants (Lypercosium esculentum) were investigated. The obtained product was characterized using FTIR spectroscopy, NMR spectroscopy, and energy dispersion spectroscopy (EDX) analysis, and the effects of this compound on the evolution of tomato plants (Lypercosium esculentum) were studied. We also studied the biological stability of the product by identifying some of the microorganisms that developed on the surface, given its susceptibility to biodegradation

    Occurrence of mycotoxins in different types of commercial dry dog food

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    The aim of this study was to determine the presence and the level of contamination of the most important mycotoxins (aflatoxin B1, deoxynivalenol, ochratoxin A and zearalenone) in 6 samples of extruded dry dog food found on the Romanian market. Analyses were performed by ELISA method using the test RIDASCREEN®. Although the concentrations of mycotoxin in all samples proved to respect the European legislation, with regards to animal feed, the analyses revealed a presence of aflatoxin B1, deoxynivalenol and zearalenone. These results revealed the need for further investigation into the potential risk derivind from chronic exposure to low doses of different types of mycotoxins that pet are currently exposed

    Selectivity and efficacy of thifensulfuron-methyl with adjuvant and without in control of broadleaf weeds in winter wheat

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    The research was conducted in 2020-2021, in 3 different locations (Constanța, Călărași and Teleorman) and aimed to evaluate the biological activity of the herbicide Thifensulfuron-methyl 75% WG with adjuvant and without in control of broadleaf weeds in winter wheat. The experiments were placed in randomized blocks, in 4 repetitions with a plot area of 100 m2 . The floristic composition of the winter wheat fields studied has been diversified, being present: Amaranthus retroflexus, Capsella bursa – pastoris, Centaurea cyanus, Chenopodium album, Erigeron annuus, Fumaria officinalis, Galium aparine, Lamium spp., Papaver rhoeas, Polygonum convolvulus, Sinapis arvensis, Stellaria media, Veronica spp., Viola arvensis etc. Herbicides were applied in post-emergence when weeds were in the early stages of growth and development. The herbicide Thifensulfuron-methyl 75% WG with adjuvant and without was applied at the doses of 20, 30 and 40 g/ha. The adjuvant (Trend 90 EC) was applied at 250 ml/ha. The assessments made at 10, 20 and 30 days after treatments focused the density of weeds, the percentage of soil cover, selectivity and the effectiveness compared to the untreated control. The results obtained showed that the efficacy depends on the dose applied, the type of weeds and their density on square meter. The Thifensulfuron-methyl 75% WG ensured a good efficacy in controlling of broadleaf weeds in winter wheat, the best results being obtained at the higher dose and when it was applied together with Trend 90 EC. Some weed species were insufficiently controlled at the dose of 20 g/ha: shepherd's purse, cleavers, blackbindweed, lamb's quarters etc

    The impact of salinity stress on antioxidant response and bioactive compounds of Nepeta cataria L.

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    In this study, the ability of Nepeta cataria L. to grow and synthesize bioactive compounds on soil treated with different salt concentrations was tested to evaluate the opportunity of cultivating it in soils affected by salinization. N. cataria L. was grown in soil containing specific amounts of NaCl, Na2SO4, and their mixture. After harvesting, the plants were analyzed from the morphological and physiological point of view. Salinity stress inhibited the growth, with the highest decrease of the plant yield up to about 70% in the case of salt mixture, and smaller values for the separate salt treatments. In the same time, as a defense mechanism, there was an increase of granular trichomes’ density, as observed with the scanning electron microscope. For mild concentrations of salt, the amount of chlorophyll pigments was enhanced, while for stronger salinity stress, it decreased. The opposite behavior was evidenced for the polyphenol content, as antioxidant activity was used as a protective mechanism against reactive oxygen species produced under salinity stress. The antioxidant activity was considerably higher for separate NaCl and Na2SO4 treatments than for the salt mixture variants. The results showed that the species Nepeta cataria L. reacts well to high salinity levels, with an increased content of bioactive compounds and antioxidant activity even for the highest studied salinity conditions

    Potential valorisation of Protobind 1000 as adsorbent for Pb2+ AND Zn2+

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    The adsorption of metal ions from increasing concentrations in aqueous solutions by modified straw lignin Protobind 1000 was studied. The effect of metallic ion concentrations (from 20.72 to 207.2 mg·L-1 for Pb2+ and from 6.538 to 65.38 mg·L-1 for Zn2+) and contact time (30, 60 and 90 minutes) were studied at pH = 6 and 200C. Langmuir and Freundlich isotherm equations were applied to assess equilibrium data and the kinetics of the adsorption processes were analysed using Lagergren pseudo first order and Ho&McKay pseudo second order models. The results show that the adsorption processes reached equilibrium after 90 minutes, but similar values were registered after 60 minutes. The Freundlich isotherm described the process better, denoting chemisorption with the formation of ion-lignin complex structures. The Ho&McKay model fit the adsorption data better with regression coefficients equal to 1 compared to the Lagergren model, where the regression factors varied between 0.72 and 0.95. For the maximum concentration of lead solution and the longest adsorption time of 90 minutes, the Ho&McKay model predicted an equilibrium capacity qe of 13.1406 mg·g-1 compared to the 13.1398 mg·g-1 obtained. For zinc adsorption, the same maximum concentration and time were considered, and the pseudo-second order model predicted a qe of 12.6743 mg·g-1 compared to the obtained value of 12.6714 mg·g-1. The uptake of lead was greater on 0.15 g of adsorbent (a maximum of 27.23 mg·g-1) than the zinc uptake (a maximum of 8.28 mg·g-1), for all analysed concentrations

    Potential of continuous electric current on biometrical, physiological and quality characteristics of organic tomato

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    The tomato is one of the most important species in the food sector. For farmers, the increase in yield in greenhouse conditions by keeping a high quality of fruits represents a goal which is very difficult to achieve in these conditions. Therefore, the present study evaluates the influence that a continuous electric current might have on some biometrical, physiological and quality parameters of tomato fruits. The study was carried out in a greenhouse where tomato plants belonging to Qualitet F1 hybrid were treated with different continuous electric currents, under 5 DC sources, stabilized by the laboratory 0–30 V/0–5 A. During the research, the tomato plants were exposed to different electric current intensities or voltages on the plants or in the soil (T1-0.15 A; T2-0.30 A; T3-0.45 A; T4-1.5 V; T5-1.5 V-soil). The tomato plant samples were compared with an untreated control. In order to determine the influence of a continuous electric current, observations and determinations were made on tomato plants and fruits. The results highlighted significant differences between the treated and not treated plants, regarding the plant height, yield, firmness, acidity, total soluble solids, antioxidant activity, crude and dietary fibres, tannins, oxalates, saponins, -amylase inhibitors, K, Mg, Fe and Zn content. Depending on the intensity of the electric current and the manner of application, the biometrical, physiological and quality parameters of tomato fruits were differently influenced. Both positive and negative influences were registered. More experiments are needed in order to establish a relation between the electric current intensity and the manner of application which can lead to better and higher tomato yields and quality in greenhouse conditions

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