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    5043 research outputs found

    Malignancy – aspects to consider in histopathological diagnosis

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    The histopathological exam is the most important tool when formulating a diagnosis in tumoral diseases. When we consider the examination of a tumor, the prognosis of the disease often depends of the character of that neoplasia (benign or malignant). To properly evaluate the future behavior of a neoplasia means to carefully observe and characterize several specific features. An abnormal differentiation degree of the cells can sometimes determine the presence of abnormal, monstruous cells, with little homogeneity between tumoral cells and a high rate of anisocytosis, anisokaryosis, anisonucleoliosis, multiple nuclei and various types of indentations or expansions of the structures of the cells. The mitotic index is another factor to take into consideration. A high mitotic index will always indicate a malignancy, and if it is associated with abnormal mitotic figures, the severity of the disease is even worse. Along with a multitude of other factors that need to be evaluated when establishing a diagnosis, from a histopathological point of view, the two mentioned criteria are the ones that need the most attention

    Effect of different boron application on cotton yield components and fiber quality properties

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    This study was carried out to determine the effect of different boron application methods on cotton yield, plant growth and fiber technological properties. The study was conducted at Siirt University Faculty of Agriculture Department of Field Crops experimental area as randomized complete block design with four replications in 2018. Stoneville 468 cotton variety and boron liquid foliar fertilizer were used as material. Seven different boron applications were performed as (Control, 1000 cc ha-1 at pre-flowering stage, 2000 cc ha-1 at preflowering stage, 1000 cc ha-1 at flowering stage, 2000 cc ha-1 at flowering stage and 1000 cc ha-1 at boll formation stage, 2000 cc ha-1 at boll formation stage). The results of statistical analysis showed that there were significant differences between applications methods in terms of plant height and number of monopodial branches. But there were non-significant differences in terms of number of sympodial branches, number of first node of sympodial branch, number of nodes, height/node ratio, number of bolls, boll weight, seed cotton weight of per boll, number of seeds per boll, first picking percentage, 100 seeds weight, ginning percentage, seed cotton yield and fiber technological characteristics. In conclusion different application methods of boron increased plant height and number of monopodial branches of cotton when applied as 1000 and 2000 cc ha-1 at preflowering stages

    Performance evaluation in the growing of the stellate sturgeon fry in 35 days from hatching under the circumstances of enrichening food with probiotics

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    The main objective of the present paper was to evaluate the influence of the commercial probiotic BetaPlus® Ultra on the growth performance of the stellate sturgeon after 35 days of hatching, but the specific objective was to evaluate the food retention efficiency in the transition period from feeding with natural feed to that with commercial feed. For this purpose, a 93-day experiment was carried out with 6 experimental variants (5 different probiotic concentrations : V 1-1.28x1013 CFU, V2-2.56 x1013 CFU, V3 -3.84x1013 CFU, V4 – 5.12x1013 CFU, V5 -6.4x1013 CFU and V6- control variant, in duplicates). The experiment started with a number of 6000 exemplars of 35-day post hatched larvae (500 specimens/rearing unit) with a mean individual weight (± SD) of 0.35 ± 0.02 g and a total length between 3-5 cm. According to the rearing technology, sorting was carried out throughout the experimental period which led to the division of the experiment into 9 different experimental stages according to feeding protocol. Feeding efficiency, expressed by FCR and protein retention, indicated by PER shows an insignificant increase (p> 0.05) in the V2 variant and an insignificant decrease in the case where the probiotic (V6) was not administered. Therefore, according to this experiment, a concentration of 2.56x1013 CFU / kg of commercial probiotic BetaPlus® Ultra represents an optimum value for promoting the growth, feeding efficiency and retention of proteins of trout after more than one gram

    Analiza unor compuşi volatili din vinurile de Aligoté, obţinute prin diferite volume de fermentaţie

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    Volatile compounds are an important factor in defining the quality of wines. Of these, the most important are: alcohols, acids, esters, ketones and terpenes. The purpose of this paper is the determination the aromatic profile of the wine obtained from the Aligoté variety, in Bucium vineyard, from Iasi. Processing of the samples was made by the stages specific to the production of quality white wines and the volatile compound determination was performed with Shimadzu HS20trap - GC 2010plus - MS040TQ, by gas chromatographic method

    Evaluation of the conservative agriculture benefits on soil properties and harvests in crop rotation with legumes

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    The experimental results regarding the changes in the properties of degraded arable ordinary chernozem from southern zone of Moldova and agricultural crops harvests in 5-field crop rotation with incorporation into the soil of one and two harvests of vetch green mass as organic fertilizer are presented. The results carried out in 2015-2019 showed that the introduction in the first agricultural year by disking into the soil of two green mass of vetch, led to restoration of the physico-chemical properties of the arable layer 0-20 cm and increased crop productions. The bulk density of 0-20 cm soil layer from unfavorable state became very favorable, the resistance to penetration from high and very high, became extremely low and low, which contributed to the easy penetration into soil of plant roots. The soil structure became agronomical favorable. The hydrostability of soil aggregates not soil layers 0-10 and 0–20 cm did not change, that is explained by the texture peculiarities of ordinary chernozem (loamy-dusty with high fine sand content). The porosity values correlate with the bulk density are favorable for a normal regime of soil aeration. The humus content increased by 0.16-0.26% in five years. The money value of the 4-year harvest increase was 18 080 MDL or 1090$. The results conducted in 2015-2019 confirmed that the preventive restoration of the quality state of the degraded arable soils is absolutely necessary to be carried out until the implementation or in the process of using the conservative agriculture system, based on No-till or Minimum-till technologies

    A case of mixed granulomatous inflammation in a turkey hen

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    Granulomatous inflammation may be caused in birds either by various infectious diseases (with the formation of Langhans type giant cells) or by the presence in different tissues and organs of foreign bodies (with the formation of Muller type giant cells), the most common being uric acid crystals in gout. We examined the cadaver of a two year old turkey hen that had multiple yellowish-white nodular formations located in the liver. Histopathological examination showed that these were formed mainly through an infectious type granulomatous inflammation caused by a chronic colibacillosis with the presence of bacterial colonies and necrotic debris in the center and Langhans giant cells with foamy cytoplasm. The liver also had early stage foreign body granulomas caused by the precipitation of uric acid crystals in the parenchyma. We were then able to establish that the bird suffered from both coligranulomatosis and also visceral gout, as a consequence of improper microclimate and feeding parameters. It is uncommon to find both types of granulomatous inflammations in the same patient or even in the same organ. The tophy gout had not yet started to form Muller type giant cells, but still, the architecture of the granulomas was clear enough to be compared to the adiacent infectious type one

    Seed germination and seedling establishment of Cherimoya (Annona Cherimola mill.) at different temperatures

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    Cherimoya (Annona cherimola Mill.) has an exceptional flavor and aroma, which makes it a fruit with great potential. However, little is known about its propagation by seeds. According to the scientific literature, the germination of cherimoya seeds is affected much more by external conditions than by internal conditions. Germination of cherimoya variety ‘Concha Lisa’ were tested for germination at constant temperatures of 25, 30, 40°C, and at room temperature, varying from 20-25°C, coupled with total darkness. Seeds were sown in Petri dishes (0.8% agar water), for 25 days of incubation. The kinetics of germination was determined according to five closely related parameters, viz. final germination percentage (FGP), mean germination time (MGT), coefficient of velocity of germination (CVG), time to 50% germination (T50) and seedling length (SL). The temperature of 30°C was found optimally suitable with 70.8% FGP, 17.5 days MGT and 3.91 cm SL, while the room temperature of 20-25°C slightly improved germination with only 25% FGP. Furthermore, significant decrease in FGP and SL was observed at 25°C and 40°C of temperature in comparison to 30°C. The analysis also revealed that cherimoya seed germination, day 10-15 after seed sowing is suitable for final counts. An overview on the emergence of cherimoya seedlings, during a 12-week period in pots is presented

    Adaptive performance of garlic varieties under high Ganges River floodplain soil (calcareous) of Bangladesh

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    Any variety needs to evaluate at different locations, along with variable soils for asses their yield potentiality after its releasing. Thus, an experiment was conducted at Multi Location Testing (MLT) site, Kushtia Sadar Upazila, under High Ganges River Floodplain (AEZ-11) in Bangladesh, during three consecutive Rabi seasons of 2013-2014 to 2015-2016. The objective of this study was to evaluate the performances of garlic varieties and economic profitability of these varieties at farmer’s field. There were three garlic varieties, namely BARI Rashun-1, BARI Rashun-2 and a local cultivar, which were evaluated at six dispersed locations as replications. Results observed that the highest yield (8.34-9.80 t ha-1) was obtained from BARI Rashun-1, which was followed by BARI Rashun-2 (7.43-9.48 t ha-1) and the local one (7.12-9.15 t ha-1). BARI Rashun-1 produced 3-12% higher yield over BARI Rashun-2 and 7-20% over the local cultivar, respectively in three consecutive years. Correlation analysis showed that there were positive and significant correlation among bulb yields with bulb length and individual bulb weight. Regarding the functional relationship, the traits like crop duration, plant population, plant height, bulb length, bulb width, individual bulb weight had positive contribution on the bulb yield. It was dependent on those traits, and accounted for 12, 36, 0.05, 45, 41 and 55% of the total bulb yield variation, respectively. The highest gross return (Tk. 3, 63,700 ha-1) and gross margin (Tk. 2,19,425 ha-1) were obtained from BARI Rashun-1. As such, this variety performed as the best, in respect of higher bulb yield potential, as well as the highest economic return among the tested varieties, which was followed by BARI Rashun-2. Therefore, the result of this study could be helpful for improving bulb production of garlic under High Ganges River Floodplain soil (calcareous soils) in Bangladesh

    Broomrape (Orobanche cumana wallr.) control, by developing genetic resistant genotypes in sunflower

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    Sunflower broomrape (Orobanche cumana Wallr.) is a parasitic plant which has a significant negative impact on seed yield. The parasite is spread in large areas of Europe, Asia and it has identified recently, in North Africa. Breeding for resistance is regarded as the most effective, feasible and environmentally friendly solution to control sunflower broomrape. However, breeding for resistance is challenging as new races of the parasite have evolved. The use of resistant hybrids of monogenic resistance type, is followed by the appearance of new more virulent races that overcome the existing resistance genes. So, it is necessary to develop sunflower hybrids which can accumulate qualitative and quantitative resistance in a single one, in order to have a durable resistance. Among this, by developing Clearfield Production System in sunflower it could have an important control strategy and complemented the genetic resistance against the parasite

    Diagnosis of Pneumocystis jirovecii Pneumonia in Pediatric Patients in Serbia, Greece, and Romania. Current Status and Challenges for Collaboration

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    Pneumocystis jirovecii can cause fatal Pneumocystis pneumonia (PcP). Many children have been exposed to the fungus and are colonized in early age, while some individuals at high risk for fungal infections may develop PcP, a disease that is difficult to diagnose. Insufficient laboratory availability, lack of knowledge, and local epidemiology gaps make the problem more serious. Traditionally, the diagnosis is based on microscopic visualization of Pneumocystis in respiratory specimens. The molecular diagnosis is important but not widely used. The aim of this study was to collect initial indicative data from Serbia, Greece, and Romania concerning pediatric patients with suspected PcP in order to: find the key underlying diseases, determine current clinical and laboratory practices, and try to propose an integrative future molecular perspective based on regional collaboration. Data were collected by the search of literature and the use of an online questionnaire, filled by relevant scientists specialized in the field. All three countries presented similar clinical practices in terms of PcP prophylaxis and clinical suspicion. In Serbia and Greece the hematology/oncology diseases are the main risks, while in Romania HIV infection is an additional risk. Molecular diagnosis is available only in Greece. PcP seems to be under-diagnosed and regional collaboration in the field of laboratory diagnosis with an emphasis on molecular approaches may help to cover the gaps and improve the practices

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