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    Criminal prosecution activity and investigations on economic crimes assigned to the state fiscal service

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    The economic activity of the state, regardless of the social and political order of the state, is one of the main links of existence and functioning of the state. It is in the conduct of external economic relations that the main financial sources of the state are rotated. Article 126 of the Constitution, characterizing the economy of the Republic of Moldova as a market economy, considers the national economy, in the same regulatory context, it is stated that the state must ensure, among others: freedom of trade and entrepreneurship; protection of fair competition; creating a favorable framework for capitalizing on all factors of production; protection of national interests in economic, financial and foreign exchange activity, etc. For these reasons, it is absolutely necessary to create in this field the legal mechanism for regulating these relations, which does not affect external economic relations, does not affect the economic potential of the state, does not tarnish the image of the state in the world arena, etc. This article is the evidence of particularities, specific features in the field of economic crimes, assigned to the State Tax Service, given that in this criminal sector, the administration of evidence involves certain specific coordinates that are not found in the investigation of other crimes. The evolution of the criminal legislation that provides for criminal liability for economic crimes is inextricably linked and conditioned by the history of our state

    Money laundering within the illegal wildlife trade: how can financial institutions play a role in combatting this negative phenomenon?

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    Each year wild animals are killed cruelly for their teeth, horn, furs and other body parts which are used for art, medicine, jewellery, antiques style furniture, etc. The criminal profits gained from the illegal wild life trade (IWT) being estimated between 7billionand7 billion and 23 billion. More and more, the IWT is considered a transnational organized crime, generating high profits for criminals involved in this activity. The “dirty” money obtained from IWT is introduced in financial institutions, moved from one jurisdiction to another, hiding their origin and laundering them using the vulnerabilities of financial institutions. This scientific research has the purpose to highlight the risks associated with IWT, to describe the IWT supply chain – the flow of illicit products from the source to the end customer, to present the IWT red flags and reporting suspicious activities, to highlight the role of financial institutions in combatting the phenomenon of IWT and to propose measures how to prevent money laundering connected to illegal wildlife trade

    Farmed cyprinids diseases from the Prut River Basin

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    The current paper aims to outline the most common fish diseases that affect farmed cyprinids in ponds from the Prut river basin, in order to better understand and reduce fish health risks. After 2 field work expeditions to Rompescaris farm -Podu-Iloaiei from Iasi county and to Dracșani fish farm from Piscicola-Botoșani county, biological samples were collected, in May 2021. Using net fishing the following fish species were harvested: carp (Cyprinus carpio), silver carp (Hypophthalmichthys molitrix) and mirror carp (Cyprinus carpio var. specularis). the biological material, the moribund specimens with visible lesions were isolated and the apparently healthy specimens were released. The collected specimens were subjected to a clinical, a parasitological and a bacteriological investigation

    Stress induced by pre-slaughter farm conditions in pigs

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    Cortisol is a steroid hormone, one of the glucocorticoids, made in the cortex of the adrenal glands and then released into the blood, which transports it in the entire body. Almost every cell contains receptors for cortisol and so cortisol can have lots of different actions depending on which sort of cells it is acting upon. These effects include controlling the body’s blood sugar levels and thus regulating metabolism, acting as an anti-inflammatory product, controlling salt and water balance and influencing blood pressure. The study was conducted over a period of 3 months, between March-August 2020, in 2 swine farms in Iasi county, Romania, on a total of 46 pigs, 3 to 4 months old, both males and females, in order to investigate stress levels in finishing facilities. The study revealed higher levels of cortisol while eosinophil counts severely decreased, changes which are associated with a strong reaction to stress for individuals that were housed in finishing facilities

    In vitro antifungal activity of silver nanoparticles biosynthesized with beech bark extract

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    Biosynthesis is a green method for the synthesis of silver nanoparticles (AgNPs). This study aimed to assess the antifungal activity of two silver nanoparticle solutions, synthesized using beech bark extract (BBE) and acetate and nitrate silver salts (AgNP Acetate BBE and AgNP Nitrate BBE), their influence on biofilm production, their potential synergistic effects with fluconazole, on different Candida spp., and their influence on virulence factors of C. albicans (germ tube production, gene expression for ALS3, SAP2, HSP70). Both the AgNP BBEs presented different minimum inhibitory concentrations for all the studied Candida spp., but biofilm production was inhibited only for C. albicans and C. guilliermondii. The growth rates of all the studied Candida spp. were inhibited in the presence of both AgNP BBEs, except for C. auris. Synergistic activity was observed for C. parapsilosis and C. guilliermondii, for different combinations of fluconazole with both the AgNP BBEs. The germ tube production of C. albicans was slightly inhibited by the AgNP BBEs. Only AgNP Acetate BBE was able to down-regulate the expression of SAP2. Overall, we can conclude that, even if more studies are necessary, AgNPs synthesized with beech bark extract might be an interesting alternative to classic antifungal treatments

    Seed dormancy breaking of an endangered medicinal tree species (Taxus baccata L.) using embryo culture

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    Natural regeneration of Taxus baccata L. is constrained due to the depth of seed dormancy requirements (often taking two or more years) and low seed germination. Further, the conventional method of vegetative propagation by cuttings is associated with difficulties in rooting. Hence, for the first time, this study describes an efficient and reproducible in vitro protocol for breaking the dormancy of seeds from the endangered forest tree T. baccata L. via zygotic embryo culture. Embryos isolated from 100% sterile seeds were cultured on DCR medium that contains sucrose (30 g/l), agar (8 g/l), and activated charcoal (5 g/l), fortified with different concentrations of Plant Growth Regulators (PGRs), and held at a temperature of 25 ± 2 ºC in a growth room. The results revealed that the in vitro embryo germination percentage was mostly affected by gibberellic acid (GA3) and thidiazuron (TDZ). Among the nine treatments, the treatments with 0.5 mg/l TDZ and 1 mg/l GA3 showed the highest germination (100%), while the other treatments all increased the germination percentages significantly compared to the control (37.5%). The 1/2 DCR medium with the addition of 0.1 mg/l indole-3-butyric acid (IBA) resulted in the highest rooting ratio (94%). However, the greatest root and hypocotyl elongation (59.37 ± 3.77 and 62.75 ± 4.43 mm, respectively) occurred when seedlings were cultured on 1/2 DCR medium containing 0.5 mg/l BA. Plantlets were transplanted into plastic pots containing an autoclaved garden soil, sand, and vermiculite mixture (1:1:1) and held at a temperature of 25 ± 2 ºC in a growth room for 4 weeks before being transplanted into the greenhouse. These results indicated that the protocol developed during the current study will be useful to overcome seed dormancy and for multiplication and conservation of the species T. baccata L

    Toxicity assessment of long-term exposure to non-thermal plasma activated water in mice

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    Non-thermal plasma activated water (PAW) has recently emerged as a powerful antimicrobial agent. Despite numerous potential bio-medical applications, studies concerning toxicity in live animals, especially after long-term exposure, are scarce. Our study aimed to assess the effects of long-term watering with PAWon the health of CD1 mice. PAWwas prepared from distilled water with a GlidArc reactor according to a previously published protocol. The pH was 2.78. The mice received PAW(experimental group) or tap water (control group) daily for 90 days as the sole water source. After 90 days, the following investigations were performed on the euthanatized animals: gross necropsy, teeth mineral composition, histopathology, immunohistochemistry, hematology, blood biochemistry, methemoglobin level and cytokine profile. Mice tolerated PAWvery well and no adverse effects were observed during the entire period of the experiment. Histopathological examination of the organs and tissues did not reveal any structural changes. Moreover, the expression of proliferation markers PCNA and Ki67 has not been identified in the epithelium of the upper digestive tract, indicating the absence of any pre- or neoplastic transformations. The results of our study demonstrated that long-term exposure to PAWcaused no toxic effects and could be used as oral antiseptic solution in dental medicine

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