RIULS (Repository of Iași University of Life Sciences)
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Criminal prosecution activity and investigations on economic crimes assigned to the state fiscal service
The economic activity of the state, regardless of the social and political order of the state, is one of the main links of
existence and functioning of the state. It is in the conduct of external economic relations that the main financial sources
of the state are rotated. Article 126 of the Constitution, characterizing the economy of the Republic of Moldova as a
market economy, considers the national economy, in the same regulatory context, it is stated that the state must ensure,
among others: freedom of trade and entrepreneurship; protection of fair competition; creating a favorable framework for
capitalizing on all factors of production; protection of national interests in economic, financial and foreign exchange
activity, etc. For these reasons, it is absolutely necessary to create in this field the legal mechanism for regulating these
relations, which does not affect external economic relations, does not affect the economic potential of the state, does not
tarnish the image of the state in the world arena, etc. This article is the evidence of particularities, specific features in
the field of economic crimes, assigned to the State Tax Service, given that in this criminal sector, the administration of
evidence involves certain specific coordinates that are not found in the investigation of other crimes. The evolution of
the criminal legislation that provides for criminal liability for economic crimes is inextricably linked and conditioned by
the history of our state
Epidemiological and etiological investigations on hepatitis E virus infection in wild animals and assessment of zoonotic risk
Money laundering within the illegal wildlife trade: how can financial institutions play a role in combatting this negative phenomenon?
Each year wild animals are killed cruelly for their teeth, horn, furs and other body parts which are used for art,
medicine, jewellery, antiques style furniture, etc. The criminal profits gained from the illegal wild life trade (IWT)
being estimated between 23 billion. More and more, the IWT is considered a transnational organized
crime, generating high profits for criminals involved in this activity. The “dirty” money obtained from IWT is
introduced in financial institutions, moved from one jurisdiction to another, hiding their origin and laundering them
using the vulnerabilities of financial institutions. This scientific research has the purpose to highlight the risks
associated with IWT, to describe the IWT supply chain – the flow of illicit products from the source to the end
customer, to present the IWT red flags and reporting suspicious activities, to highlight the role of financial institutions
in combatting the phenomenon of IWT and to propose measures how to prevent money laundering connected to illegal
wildlife trade
Farmed cyprinids diseases from the Prut River Basin
The current paper aims to outline the most common fish diseases that affect farmed cyprinids in ponds from the Prut river basin,
in order to better understand and reduce fish health risks. After 2 field work expeditions to Rompescaris farm -Podu-Iloaiei
from Iasi county and to Dracșani fish farm from Piscicola-Botoșani county, biological samples were collected, in May
2021. Using net fishing the following fish species were harvested: carp (Cyprinus carpio), silver carp
(Hypophthalmichthys molitrix) and mirror carp (Cyprinus carpio var. specularis). the biological material, the moribund
specimens with visible lesions were isolated and the apparently healthy specimens were released. The collected specimens
were subjected to a clinical, a parasitological and a bacteriological investigation
Stress induced by pre-slaughter farm conditions in pigs
Cortisol is a steroid hormone, one of the glucocorticoids, made in the cortex of the adrenal glands and then released into the blood, which transports it in the entire body. Almost every cell contains receptors for cortisol and so cortisol can have lots of different actions depending on which sort of cells it is acting upon. These effects include controlling the body’s blood sugar levels and thus regulating metabolism, acting as an anti-inflammatory product, controlling salt and water balance and influencing blood pressure. The study was conducted over a period of 3 months, between March-August 2020, in 2 swine farms in Iasi county, Romania, on a total of 46 pigs, 3 to 4 months old, both males and females, in order to investigate stress levels in finishing facilities. The study revealed higher levels of cortisol while eosinophil counts severely decreased, changes which are associated with a strong reaction to stress for individuals that were housed in finishing facilities
In vitro antifungal activity of silver nanoparticles biosynthesized with beech bark extract
Biosynthesis is a green method for the synthesis of silver nanoparticles (AgNPs). This
study aimed to assess the antifungal activity of two silver nanoparticle solutions, synthesized using
beech bark extract (BBE) and acetate and nitrate silver salts (AgNP Acetate BBE and AgNP Nitrate
BBE), their influence on biofilm production, their potential synergistic effects with fluconazole, on
different Candida spp., and their influence on virulence factors of C. albicans (germ tube production,
gene expression for ALS3, SAP2, HSP70). Both the AgNP BBEs presented different minimum
inhibitory concentrations for all the studied Candida spp., but biofilm production was inhibited
only for C. albicans and C. guilliermondii. The growth rates of all the studied Candida spp. were
inhibited in the presence of both AgNP BBEs, except for C. auris. Synergistic activity was observed
for C. parapsilosis and C. guilliermondii, for different combinations of fluconazole with both the AgNP
BBEs. The germ tube production of C. albicans was slightly inhibited by the AgNP BBEs. Only AgNP
Acetate BBE was able to down-regulate the expression of SAP2. Overall, we can conclude that, even
if more studies are necessary, AgNPs synthesized with beech bark extract might be an interesting
alternative to classic antifungal treatments
Seed dormancy breaking of an endangered medicinal tree species (Taxus baccata L.) using embryo culture
Natural regeneration of
Taxus baccata L. is constrained due to the
depth of seed dormancy requirements
(often taking two or more years) and low
seed germination. Further, the
conventional method of vegetative
propagation by cuttings is associated with
difficulties in rooting. Hence, for the first
time, this study describes an efficient and
reproducible in vitro protocol for breaking
the dormancy of seeds from the
endangered forest tree T. baccata L. via
zygotic embryo culture. Embryos isolated
from 100% sterile seeds were cultured on
DCR medium that contains sucrose (30 g/l),
agar (8 g/l), and activated charcoal (5 g/l),
fortified with different concentrations of
Plant Growth Regulators (PGRs), and
held at a temperature of 25 ± 2 ºC in a
growth room. The results revealed that the
in vitro embryo germination percentage
was mostly affected by gibberellic acid
(GA3) and thidiazuron (TDZ). Among the
nine treatments, the treatments with
0.5 mg/l TDZ and 1 mg/l GA3 showed
the highest germination (100%), while the
other treatments all increased the
germination percentages significantly
compared to the control (37.5%). The 1/2
DCR medium with the addition of 0.1
mg/l indole-3-butyric acid (IBA) resulted
in the highest rooting ratio (94%).
However, the greatest root and hypocotyl
elongation (59.37 ± 3.77 and 62.75 ±
4.43 mm, respectively) occurred when
seedlings were cultured on 1/2 DCR
medium containing 0.5 mg/l BA. Plantlets
were transplanted into plastic pots
containing an autoclaved garden soil,
sand, and vermiculite mixture (1:1:1) and
held at a temperature of 25 ± 2 ºC in a
growth room for 4 weeks before being
transplanted into the greenhouse. These
results indicated that the protocol
developed during the current study will be
useful to overcome seed dormancy and
for multiplication and conservation of the
species T. baccata L
Toxicity assessment of long-term exposure to non-thermal plasma activated water in mice
Non-thermal plasma activated water (PAW) has recently emerged as a powerful antimicrobial
agent. Despite numerous potential bio-medical applications, studies concerning toxicity in
live animals, especially after long-term exposure, are scarce. Our study aimed to assess the effects of
long-term watering with PAWon the health of CD1 mice. PAWwas prepared from distilled water
with a GlidArc reactor according to a previously published protocol. The pH was 2.78. The mice
received PAW(experimental group) or tap water (control group) daily for 90 days as the sole water
source. After 90 days, the following investigations were performed on the euthanatized animals:
gross necropsy, teeth mineral composition, histopathology, immunohistochemistry, hematology,
blood biochemistry, methemoglobin level and cytokine profile. Mice tolerated PAWvery well and
no adverse effects were observed during the entire period of the experiment. Histopathological
examination of the organs and tissues did not reveal any structural changes. Moreover, the expression
of proliferation markers PCNA and Ki67 has not been identified in the epithelium of the upper
digestive tract, indicating the absence of any pre- or neoplastic transformations. The results of our
study demonstrated that long-term exposure to PAWcaused no toxic effects and could be used as
oral antiseptic solution in dental medicine