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移植免疫寛容霊長類モデルにおけるiPS細胞を用いた心筋再生療法の開発
No full text研究種目：基盤研究(B)研究期間：2014～2016課題番号：26293182研究代表者：柴 祐司研究者番号：70613503Other2014～2016年度科学研究費助成事業（基盤研究(B)）研究成果報告書 課題番号：26293182 研究代表者：柴 祐司research repor
血管障害後の新生内膜肥厚形成におけるマクロファージ由来のヒアルロン酸の重要性
No full text研究種目：若手研究(B)研究期間：2014～2016課題番号：26860550研究代表者：嘉嶋 勇一郎研究者番号：70545722Other2014～2016年度科学研究費助成事業（若手研究(B)）研究成果報告書 課題番号：26860550 研究代表者：嘉嶋 勇一郎research repor
メラノーマの腫瘍内および腫瘍間におけるBRAF変異の多様性の解析
No full text研究種目：若手研究(B)研究期間：2015～2016課題番号：15K19685研究代表者：境澤 香里研究者番号：20419352Other2015～2016年度科学研究費助成事業（若手研究(B)）研究成果報告書 課題番号：15K19685 研究代表者：境澤 香里research repor
Brown Nodule on the Lower Eyelid: A Quiz Lichenoid keratosis
No full textArticleACTA DERMATO-VENEREOLOGICA.95(8):1037-1038(2015)journal articl
Toll-Like Receptor 4 Signaling Promotes the Migration of Human Melanoma Cells
No full textImmune cell Toll-like receptors (TLRs) recognize conserved microbial components, leading to immune and inflammatory responses. However, TLRs are also expressed in cancer cells, including melanoma cells, which express TLR2-4. TLR4 ligands have received attention as immunotherapies; therefore, we assessed the expression of TLR4 in human melanoma specimens (29 primary lesions and 28 metastatic lesions) representing different types of melanoma. A high percentage (≥ 90%) of melanoma lesions expressed TLR4, as judged by immunohistochemistry. Next, the role of TLR4 in cell proliferation and migration was assessed using the TLR4-positive (TLR4+) melanoma cell lines 501mel and 888mel, and TLR4-negative (TLR4‒) 928mel melanoma cells. Lipopolysaccharide (LPS), a TLR4 agonist, increased the proliferation of TLR4+ melanoma cells but not of TLR4‒ 928mel cells. The proliferation-inducing effect of LPS in 888mel cells was abolished by blockade of TLR4 signaling via treatment with short interfering RNA (siRNA) targeting TLR4 or myeloid differentiation primary response gene 88 (MyD88), a molecule downstream of TLR4. However, knockdown of TLR4 or MyD88 expression did not affect the LPS-induced proliferation of 501mel cells, suggesting that residual TLR4 signaling is sufficient to maintain cell proliferation. By contrast, LPS increased the migration of TLR4+ melanoma cells, and this effect was substantially inhibited by TLR4 or MyD88 knockdown. Furthermore, TLR4 knockdown decreased cell migration even in the absence of LPS, suggesting the presence of an endogenous TLR4 ligand(s) in melanoma cells. TLR4 signaling may contribute to melanoma progression, and caution should be exercised when using TLR4 ligands as adjuvant therapy for cancer.ArticleTOHOKU JOURNAL OF EXPERIMENTAL MEDICINE.234(1):57-65(2014)journal articl
電動アシストエルゴメーターのペダルスピードによる筋電図解析と透析患者の自覚症状
No full text研究種目：挑戦的萌芽研究研究期間：2014～2016課題番号：26560304研究代表者：三好 圭研究者番号：30293509Other2014～2016年度科学研究費助成事業（挑戦的萌芽研究）研究成果報告書 課題番号：26560304 研究代表者：三好 圭research repor
Changes in synaptic transmission of substantia gelatinosa neurons after spinal cord hemisection revealed by analysis using in vivo patch-clamp recording
No full textBackgroundAfter spinal cord injury, central neuropathic pain develops in the majority of spinal cord injury patients. Spinal hemisection in rats, which has been developed as an animal model of spinal cord injury in humans, results in hyperexcitation of spinal dorsal horn neurons soon after the hemisection and thereafter. The hyperexcitation is likely caused by permanent elimination of the descending pain systems. We examined the change in synaptic transmission of substantia gelatinosa neurons following acute spinal hemisection by using an in vivo whole-cell patch-clamp technique.ResultsAn increased spontaneous action potential firings of substantia gelatinosa neurons was detected in hemisected rats compared with that in control animals. The frequencies and amplitudes of spontaneous excitatory postsynaptic currents and of evoked excitatory postsynaptic currentss in response to non-noxious and noxious stimuli were not different between hemisected and control animals. On the contrary, the amplitude and frequency of spontaneous inhibitory postsynaptic currents of substantia gelatinosa neurons in hemisected animals were significantly smaller and lower, respectively, than those in control animals (P < 0.01). Large amplitude and high-frequency spontaneous inhibitory postsynaptic currents, which could not be elicited by mechanical stimuli, were seen in 44% of substantia gelatinosa neurons in control animals but only in 17% of substantia gelatinosa neurons in hemisected animals. In control animals, such large amplitude spontaneous inhibitory postsynaptic currents were suppressed by spinal application of tetrodotoxin (1 µM). Cervical application of lidocaine (2%, 10 µl) also inhibited such large amplitude of inhibitory postsynaptic currents. The proportion of multi-receptive substantia gelatinosa neurons, which exhibit action potential firing in response to non-noxious and noxious stimuli, was much larger in hemisected animals than in control animals.ConclusionsThese suggest that substantia gelatinosa neurons receive tonic inhibition by spinal inhibitory interneurons which generate persistent action potentials. Spinal hemisection results in hyperexcitation of substantia gelatinosa neurons at least in part by eliminating the tonic descending control of spinal inhibitory interneurons from supraspinal levelsArticleMOLECULAR PAIN.12:1744806916665820(2016)journal articl
Conscious Sedation and Awake Fiberoptic Intubation in a Patient with Difficult Mask Ventilation―A Case Report
No full textSince maxillofacial malignancy is a common cause of facial defects and disfigurement of the face that may make fitting of a mask difficult and cause air leakage from the side, thus making mask ventilation difficult. In addition, distorted anatomy of the airway and base of the skull in such patients may cause difficult intubation (DI). We experienced a case with a huge facial defect due to maxillary carcinoma, in which difficult mask ventilation (DMV) and DI were predicted. After evaluation by three-dimensional airway computed tomography, the airway was secured with conscious sedation using dexmedetomidine, and awake fiberoptic intubation was safely performed. Three-dimensional airway computed tomography seems to be a good tool for successful intubation when DMV and DI are predicted.ArticleOpen Journal of Anesthesiology.5(9):60079(2015)journal articl
Calciphylaxis in POEMS Syndrome: A Case Treated with Etidronate
No full textArticleACTA DERMATO-VENEREOLOGICA.91(1):98-99(2011)journal articl
Deletion of Lrp4 increases the incidence of microphthalmia
No full textMicrophthalmia is a malformation that reduces the size of the ocular globe. The etiologies of this anomaly are various, but the genetic background appears to have a predominant influence on its development through mutations of genes controlling ocular developmental processes. LRP4 is a type I single transmembrane protein that is essential for the formation of neuromuscular junctions. We created and experimented on homozygous Lrp4-deficient mice and found the microphthalmia phenotype in their eyes. The loss of Lrp4 resulted in an elevated incidence of microphthalmia and affected the mRNA expression of the members of bone morphogenetic protein, fibroblast growth factor, Sonic hedgehog, and WNT signaling pathways and of several pathogenic genes for microphthalmia. Moreover, the loss of Lrp4 enhanced the incidence of aberrant retinal folds, which appeared pleated and corrugated in the eyeball.ArticleBiochemical and Biophysical Research Communications. 506(2018)journal articl