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Fenretinide Causes Emphysema, Which Is Prevented by Sphingosine 1-Phoshate
No full textSphingolipids play a role in the development of emphysema and ceramide levels are increased in experimental models of emphysema; however, the mechanisms of ceramide-related pulmonary emphysema are not fully understood. Here we examine mechanisms of ceramide-induced pulmonary emphysema. Male Sprague-Dawley rats were treated with fenretinide (20 mg/kg BW), a synthetic derivative of retinoic acid that causes the formation of ceramide, and we postulated that the effects of fenretinide could be offset by administering sphingosine 1-phosphate (S1P) (100 µg/kg BW). Lung tissues were analyzed and mean alveolar airspace area, total length of the alveolar perimeter and the number of caspase-3 positive cells were measured. Hypoxia-inducible factor alpha (HIF-1α), vascular endothelial growth factor (VEGF) and other related proteins were analyzed by Western blot analysis. Immunohistochemical analysis of HIF-1α was also performed. Ceramide, dihydroceramide, S1P, and dihydro-S1P were measured by mass spectrometer. Chronic intraperitoneal injection of fenretinide increased the alveolar airspace surface area and increased the number of caspase-3 positive cells in rat lungs. Fenretinide also suppressed HIF-1α and VEGF protein expression in rat lungs. Concomitant injection of S1P prevented the decrease in the expression of HIF-1α, VEGF, histone deacetylase 2 (HDAC2), and nuclear factor (erythroid-derived 2)-like 2 (Nrf2) protein expression in the lungs. S1P injection also increased phosphorylated sphingosine kinase 1. Dihydroceramide was significantly increased by fenretinide injection and S1P treatment prevented the increase in dihydroceramide levels in rat lungs. These data support the concept that increased de novo ceramide production causes alveolar septal cell apoptosis and causes emphysema via suppressing HIF-1α. Concomitant treatment with S1P normalizes the ceramide-S1P balance in the rat lungs and increases HIF-1α protein expression via activation of sphingosine kinase 1; as a consequence, S1P salvages fenretinide induced emphysema in rat lungs.ArticlePLOS ONE.8(1):e53927(2013)journal articl
信州母子保健推進センターと保健福祉事務所の協働による乳幼児健康診査見直し支援の取組
No full text要旨：保健福祉事務所母子保健担当保健師と母子保健推進員が実際に乳幼児健診の現場に入る機会を得た。その中で乳幼児健診の実施方法等について実態を把握し外部からの視点で評価を行い、課題抽出を行った。また、その結果を村保健師等と共有し、乳幼児健診の持ち方、評価方法、健診票等について検討を行い健診全体の実施方法等の見直しにつなげることができた。Article信州公衆衛生雑誌 13(1): 46-47(2018)journal articl
Nutritional studies on improvement of phosphorus availability in chickens using buckwheat（ソバを活用したニワトリのリン利用性改善に関する栄養学的研究）
No full text信州大学(Shinshu university)博士（農学）ThesisRAKHI CHOWDHURY. Nutritional studies on improvement of phosphorus availability in chickens using buckwheat（ソバを活用したニワトリのリン利用性改善に関する栄養学的研究）. 信州大学, 2018, 博士論文. 博士（農学）, 甲第78号, 平成30年09月30日授与.doctoral thesi
A study on the immune effects of synergistic oligodeoxynucleotide from probiotics（プロバイオティクス由来相乗型オリゴDNAの免疫特性に関する研究）
No full text信州大学(Shinshu university)博士（農学）ThesisSHIREEN NIGAR. A study on the immune effects of synergistic oligodeoxynucleotide from probiotics（プロバイオティクス由来相乗型オリゴDNAの免疫特性に関する研究）. 信州大学, 2018, 博士論文. 博士（農学）, 甲第79号, 平成30年09月30日授与.doctoral thesi
マルハナバチ媒植物における花サイズおよび種多様性の標高に伴う変化 ～マルハナバチ種構成の標高間変異との関係～
No full text信州大学(Shinshu university)博士（理学）Thesis江川 信. マルハナバチ媒植物における花サイズおよび種多様性の標高に伴う変化 ～マルハナバチ種構成の標高間変異との関係～. 信州大学, 2018, 博士論文. 博士（理学）, 甲第125号, 平成30年09月30日授与.doctoral thesi
伝統食の生理機能顕在化とヒトにおける長期的な機能評価法確立のための基盤研究
No full text研究種目：基盤研究(C)研究期間：2014～2016課題番号：26350145研究代表者：横山 忠幸研究者番号：90592155Other2014～2016年度科学研究費助成事業（基盤研究(C)）研究成果報告書 課題番号：26350145 研究代表者：横山 忠幸research repor
医療的ケアを必要とする子どもの療養行動獲得支援の評価と普及に向けた研究
No full text研究種目：基盤研究(C)研究期間：2013～2016課題番号：25463524研究代表者：平林 優子研究者番号：50228813Other2013～2016年度科学研究費助成事業（基盤研究(C)）研究成果報告書 課題番号：25463524 研究代表者：平林 優子research repor
電子カルテ閲覧行為の業務関連度を示す定量的指標の作成
No full text研究種目：基盤研究(C)研究期間：2014～2016課題番号：26460859研究代表者：濱野 英明研究者番号：10447724Other2014～2016年度科学研究費助成事業（基盤研究(C)）研究成果報告書 課題番号：26460859 研究代表者：濱野 英明research repor
The Dispersion State of Tangled Multi-Walled Carbon Nanotubes Affects Their Cytotoxicity
No full textThe medical applications of carbon nanotubes (CNTs) have garnered much attention. However, evaluating the safety of CNTs remains difficult, and no consensus has been reached. Moreover, assessing the biosafety of multi-walled CNTs (MWCNTs), which can become tangled during manufacturing, is challenging because they do not readily disperse. We studied how the dispersion state of tangled MWCNTs affects their cytotoxicity, using three sonicators. Flotube 9110 (FT9110), tangled MWCNTs, were dispersed in two dispersants (fetal bovine serum and polysorbate 80) using a new type of sonicator (PR-1) and two conventional sonicators. The size and cytotoxicity of the dispersed FT9110 were measured using the BEAS-2B human bronchial epithelial cell line. The PR-1 dispersed the FT9110 to agglomerates 1000 nm in diameter, and cytotoxicity depended on the dispersant. We found that excluding cells adhered to agglomerated FT9110 before evaluating cytotoxicity can lead to false-positive results. The PR-1 sonicator dispersed tangled FT9110 to many single fibers, which showed lower cytotoxicity than conventionally-sonicated MWCNTs. We suggest that dispersion state should be accounted for when evaluating the cytotoxicity of MWCNTs.ArticleNANOMATERIALS.6(11):219(2016)journal articl