BIOREpository (Faculty of Biology, University of Belgrade)
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    Doubling genome size of energy willow affects woody stem cell wall structure, chemistry, and biogas yield

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    Effectiveness in woody biomass utilization is highly dependent on its genetics and physiology. We performed morpho-anatomical, chemical, and biomethane productivity characterizations of one-year-old woody stems in three shrub Salix viminalis genotypes: a diploid (Energo) and its two autotetraploid derivatives (PP-E7 and PP-E13). Tetraploidization affected changes in stem morpho-anatomy and corresponding improved chemical features and biomethane productivity, considerably more pronounced in tetraploid PP-E13, while PP-E7 was more similar to diploid Energo. Compared to diploid Energo, in tetraploid PP-E13 morphometric analysis showed increased stem diameter and higher wood fiber radial double wall thickness, while microscopic analysis suggested higher syringyl to guaiacyl (S:G) ratio of the wood fiber cell wall. Presented changes in stem morpho-anatomy of tetraploid PP-E13 compared to diploid Energo correspond to the improved chemical features: the lower Klason lignin content and higher S:G ratio, the higher cellulose and xylan content, and lower cellulose crystallinity (Crl). Presented improved chemical features, along with the increase in ash content, resulted in a 7.3% (10.3 CH4 mL/g VS) increase in biomethane productivity in tetraploid PP-E13, compared to diploid Energo, suggesting tetraploid PP-E13 as an optimal raw material for fermentation technologies. In addition, besides the well-known chemical markers of willow biomass quality, the presented results highlight key stem morpho-anatomical parameters, which can serve as additional markers in energy willow improvement.M21a3.11353137958

    Beneficial Effects of Ganoderma spp. on Neurodegenerative Diseases

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    The progress of medicine as well as the improvement of living conditions has increased human life expectancy. Thus, the average life span increased from 60 years at the beginning of the 20th century to even 90 years at the beginning of the 21st century. On the other hand, there are more and more patients suffering from numerous age-related neurodegenerative diseases and disorders. For example, one in three elderly people dies from some form of dementia, and millions of Alzheimer’s and Parkinson’s patients are registered worldwide. When patients with other neurodegenerative diseases are added to these numbers, it becomes clear that there is a need to improve existing therapies and find a new effective treatment. Since available conventional therapies only slow disease progression and have numerous side effects, there is a need for natural sources of anti-neurodegenerative agents. Species of the genus Ganoderma, valued as traditional remedies in the Far East, are a promising basis for new drugs as numerous studies have reported their exceptional neuroprotective potential. Numerous studies have reported the anti-neurodegenerative effects of Ganoderma spp. by inhibiting enzymes that cause Alzheimer’s and Parkinson’s diseases. Their antioxidant, anti-neuroinflammatory, anti-amnesic, anti-epileptic, antidepressant, and neuroprotective effects after stroke have also been documented.M1314816

    The first record of phoretic mite Sennertia cerambycina (Acari, Chaetodactylidae) in Serbia

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    The phoretic mite Sennertia cerambycina (Scopoli, 1763) (Acari, Chaetodactylidae) has been documented for the first time within the territory of Serbia. Deutonymphs of this species were identified on a heavily infested female violet carpenter bee, Xylocopa violacea (Linnaeus, 1758) (Hymenoptera, Apidae). The infested bee was collected on November 11th, 2023, in Vrčin, near Belgrade.M2442142467

    Enhancement of propolis food preservation and functional ingredient characteristics by natural eutectic solvents extraction of phytochemicals

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    Due to the promising applications of propolis as a natural food preservative, functional ingredient or supplement, on one side, and the inherent toxicity and high volatility of organic solvents used for extraction of phenolics from propolis, a range of natural eutectic solvents (NESs) was evaluated for the extraction of phenolic compounds from poplar type propolis to create “green-labelled” food products. The phenolic profile of novel green propolis extracts was evaluated using high-performance thin-layer chromatography, while 16 phenolic compounds were quantified by ultra-high-performance liquid chromatography hyphenated with mass spectrometry. Pinocembrin and chrysin were the most dominant phenolics with an average concentration of 3111 and 3039 mg/kg in lactic acid-based NES, respectively. Biological activity, such as antioxidative and antimicrobial activities, were determined using spectrophotometric and well diffusion methods. Considering all spectrophotometric assays lactic acid-based presented as the best extraction medium for phenolic extraction, with TPC, TFC and RSA values 150.0 mg GAE/g, 190.0 mg RUE/g, and 132.8 mg TE/g on average, respectively. From 38 studied NESs, choline-chloride:tartaric acid (1:1 n/n), glycerol:lactic acid (1:1 n/n) and glycine: lactic acid (1:3 n/n) were the most promising extraction medium for obtaining the propolis extracts enriched with bioactive compounds. Additionally, proposed green solvents were proved to be the designer solvents and showed potential to be tuned to target specific phenolic compounds. Improved, or at least maintained, biological activity of NES propolis extracts support its application in agriculture industry.M215.3181034675

    Feeding behaviour and food choice in three populations of the millipede Glomeris hexasticha Brandt, 1833 (Diplopoda: Glomerida: Glomeridae): intra- and interpopulation laboratory study

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    Foraging behaviour, determined by both internal and external factors, requires investment of time and energy. Optimizing the nutritional decisions also influences efficiency during the feeding process and fitness. Feeding behaviour has been poorly investigated in millipedes. Hence, the aims of this study were to test food choice and to explore intra- and interpopulation variability in several sequences of feeding behaviour in millipede Glomeris hexasticha Brandt, 1833 under laboratory conditions. Individuals of G. hexasticha and leaf litter were collected from three populations within Belgrade, Serbia. A feeding experiment was conducted using a cross-shaped experimental maze with oak leaves, small-leaved linden leaves, and moss as potential food sources. The individuals most often chose oak leaves, subsequently moss, while linden leaves were the least preferred. Sexual dimorphism was detected only for the average time spent in maze arms that contained food items in the Ada New Belgrade population and the Hyde Park population. Intrapopulation differences that depended on feeding status and interpopulation differences in some behavioural sequences were detected. We assume that sexual dimorphism in most of the analysed behavioural traits was not detected, because both sexes had to satisfy basic nutritive requirements after the same starvation period. However, research timing may be responsible, at least partially, for the obtained results. Repeating experiments in different periods of the year or using more fresh food or food sources in different phases of the decomposition may give different results. Further, in our experimental design, some unknown preference towards particular maze arms could not be completely excluded.M221.22711

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    BIOREpository (Faculty of Biology, University of Belgrade)
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